Homologs of GFF7320 from Variovorax sp. SCN45

tRNA-i(6)A37 methylthiotransferase (EC 2.8.4.3)
SEED: tRNA-i(6)A37 methylthiotransferase
KEGG: bifunctional enzyme involved in thiolation and methylation of tRNA

Top 100 hits from BLASTp (E < 0.01)

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Orth Species Gene Name Description Fitness %Id Cov
  Variovorax sp. SCN45 GFF7320 tRNA-i(6)A37 methylthiotransferase (EC 2.8.4.3) insig. 100 100
o Variovorax sp. OAS795 ABID97_RS06475 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 94 100
o Acidovorax sp. GW101-3H11 Ac3H11_4008 tRNA-i(6)A37 methylthiotransferase insig. 85 100
o Hydrogenophaga sp. GW460-11-11-14-LB1 GFF613 tRNA-i(6)A37 methylthiotransferase cofit 81 98
o Ralstonia solanacearum PSI07 RPSI07_RS21380 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 75 98
o Ralstonia solanacearum GMI1000 RS_RS02645 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 75 98
o Paraburkholderia sabiae LMG 24235 QEN71_RS02195 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 75 98
o Ralstonia solanacearum IBSBF1503 RALBFv3_RS11825 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 74 98
o Ralstonia solanacearum UW163 UW163_RS01760 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 74 98
o Ralstonia sp. UNC404CL21Col ABZR87_RS07780 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 74 98
o Cupriavidus basilensis FW507-4G11 RR42_RS02855 dimethylallyladenosine tRNA methylthiotransferase insig. 71 98
o Paraburkholderia bryophila 376MFSha3.1 H281DRAFT_04239 tRNA-i(6)A37 thiotransferase enzyme MiaB insig. 73 98
o Burkholderia phytofirmans PsJN BPHYT_RS16535 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase sig. 73 98
o Dechlorosoma suillum PS Dsui_1194 tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB cofit 70 98
o Herbaspirillum seropedicae SmR1 HSERO_RS03615 (dimethylallyl)adenosine tRNA methylthiotransferase insig. 71 98
o Castellaniella sp019104865 MT123 ABCV34_RS15345 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 69 98
o Rhodanobacter denitrificans MT42 LRK55_RS13465 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 69 97
o Dyella japonica UNC79MFTsu3.2 ABZR86_RS09895 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB sig. 69 98
o Lysobacter sp. OAE881 ABIE51_RS15455 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 66 98
o Rhodanobacter sp000427505 FW510-R12 LRK53_RS14850 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 67 99
o Rhodanobacter sp. FW510-T8 OKGIIK_02990 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 67 98
o Xanthomonas campestris pv. campestris strain 8004 Xcc-8004.2224.1 tRNA-i(6)A37 methylthiotransferase strong 67 98
o Rhodanobacter denitrificans FW104-10B01 LRK54_RS13750 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB sig. 66 99
o Alteromonas macleodii MIT1002 MIT1002_01480 (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB insig. 64 99
o Serratia liquefaciens MT49 IAI46_06025 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 64 99
o Kangiella aquimarina DSM 16071 B158DRAFT_0099 tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB sig. 63 98
o Rahnella sp. WP5 EX31_RS23205 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 63 99
o Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 GFF4579 tRNA-i(6)A37 methylthiotransferase strong 63 99
o Escherichia coli ECRC62 BNILDI_21610 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 63 99
o Escherichia coli BL21 ECD_00629 tRNA-i(6)A37 methylthiotransferase strong 63 99
o Escherichia coli ECOR27 NOLOHH_23605 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB strong 63 99
o Escherichia coli ECOR38 HEPCGN_01005 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB strong 63 99
o Escherichia coli ECRC100 OKFHMN_07225 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB strong 63 99
o Escherichia coli ECRC101 MCAODC_26440 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB strong 63 99
o Escherichia coli ECRC102 NIAGMN_05280 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 63 99
o Escherichia coli ECRC98 JDDGAC_10870 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB strong 63 99
o Escherichia coli ECRC99 KEDOAH_20745 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB no data 63 99
o Escherichia coli HS(pFamp)R (ATCC 700891) OHPLBJKB_03051 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase cofit 63 99
o Escherichia coli BW25113 b0661 miaB isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) sig. 63 99
o Escherichia fergusonii Becca EFB2_03438 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase insig. 63 99
o Vibrio cholerae E7946 ATCC 55056 CSW01_04915 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 65 99
o Escherichia coli Nissle 1917 ECOLIN_RS03555 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB no data 63 99
o Enterobacter asburiae PDN3 EX28DRAFT_2310 tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB insig. 62 99
o Klebsiella michiganensis M5al BWI76_RS08135 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB no data 62 99
o Pantoea sp. MT58 IAI47_13945 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 62 99
o Pantoea agglomerans CFBP13505 P0401 PagCFBP13505_RS03360 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 62 99
o Enterobacter sp. TBS_079 MPMX20_01311 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase insig. 62 99
o Shewanella amazonensis SB2B Sama_2575 tRNA-i(6)A37 modification enzyme MiaB (RefSeq) strong 62 99
o Klebsiella pneumoniae MKP103 KDGMDA_16495 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB sig. 62 99
o Pectobacterium carotovorum WPP14 HER17_RS15165 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 62 99
o Shewanella sp. ANA-3 Shewana3_1008 tRNA-i(6)A37 thiotransferase enzyme MiaB (RefSeq) strong 62 99
o Shewanella oneidensis MR-1 SO1181 miaB tRNA-i(6)A37 modification enzyme MiaB (NCBI ptt file) strong 62 99
o Pseudomonas aeruginosa MRSN321 DY961_RS12950 miaB;go_function=transferase tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 62 98
o Pseudomonas aeruginosa PA14 IKLFDK_20540 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB sig. 62 98
o Pseudomonas aeruginosa PUPa3 DQ20_RS48275 miaB;go_function=transferase tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 62 98
o Dickeya dianthicola 67-19 HGI48_RS06295 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 61 99
o Marinobacter adhaerens HP15 HP15_2466 tRNA-i(6)A37 thiotransferase enzyme MiaB strong 61 98
o Dickeya dianthicola ME23 DZA65_RS06640 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 60 99
o Pseudomonas segetis P6 ACVTMO_RS05310 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 61 98
o Shewanella loihica PV-4 Shew_2923 tRNA-i(6)A37 thiotransferase enzyme MiaB (RefSeq) strong 61 99
o Pseudomonas sp. S08-1 OH686_09215 tRNA-i(6)A37 thiotransferase enzyme MiaB insig. 62 98
o Dickeya dadantii 3937 DDA3937_RS06250 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 60 99
o Erwinia amylovora T8 OLJFJH_17145 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 60 99
o Erwinia tracheiphila HP pepo 2.2 IJEDHG_02360 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 60 99
o Erwinia tracheiphila SCR3 LU632_RS16305 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 60 99
o Pseudomonas syringae pv. syringae B728a Psyr_4345 tRNA-i(6)A37 thiotransferase enzyme MiaB sig. 61 98
o Pseudomonas syringae pv. syringae B728a ΔmexB Psyr_4345 tRNA-i(6)A37 thiotransferase enzyme MiaB insig. 61 98
o Pseudomonas fluorescens FW300-N2E2 Pf6N2E2_5018 tRNA-i(6)A37 methylthiotransferase sig. 60 98
o Pseudomonas stutzeri RCH2 Psest_0532 tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB sig. 62 98
o Pseudomonas sp. DMC3 GFF3900 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase insig. 60 98
o Pseudomonas fluorescens FW300-N2C3 AO356_15410 (dimethylallyl)adenosine tRNA methylthiotransferase insig. 60 98
o Pseudomonas orientalis W4I3 QF045_RS26910 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 60 98
o Pseudomonas sp. RS175 PFR28_03848 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase insig. 60 98
o Pseudomonas fluorescens FW300-N2E3 AO353_06375 (dimethylallyl)adenosine tRNA methylthiotransferase sig. 60 98
o Pseudomonas simiae WCS417 PS417_24625 (dimethylallyl)adenosine tRNA methylthiotransferase sig. 60 98
o Pseudomonas lactucae CFBP13502 GEMAOFIL_05078 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase insig. 60 98
o Pseudomonas fluorescens FW300-N1B4 Pf1N1B4_2712 tRNA-i(6)A37 methylthiotransferase sig. 60 98
o Pseudomonas fluorescens SBW25 PFLU_RS26555 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 60 98
o Pseudomonas fluorescens SBW25-INTG PFLU_RS26555 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB sig. 60 98
o Pseudomonas fluorescens GW456-L13 PfGW456L13_1380 tRNA-i(6)A37 methylthiotransferase insig. 60 98
o Methylophilus sp. DMC18 GFF2710 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase insig. 62 92
o Pseudomonas sp. SVBP6 COO64_RS15510 miaB tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 59 97
o Acinetobacter radioresistens SK82 MPMX26_02667 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase cofit 58 99
o Acinetobacter baumannii LAC-4 RR41_RS02450 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB cofit 57 99
  Paraburkholderia graminis OAS925 ABIE53_003479 tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB insig. 72 65
o Azospirillum sp. SherDot2 MPMX19_02511 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase insig. 49 98
o Magnetospirillum magneticum AMB-1 AMB_RS22585 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase insig. 51 95
o Agrobacterium fabrum C58 Atu0356 miaB protein sig. 48 97
o Azospirillum brasilense Sp245 AZOBR_RS01475 (dimethylallyl)adenosine tRNA methylthiotransferase insig. 48 98
o Rhizobium sp. OAE497 ABIE40_RS01705 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB strong 48 97
o Rhodospirillum rubrum S1H Rru_A3770 tRNA-i(6)A37 modification enzyme MiaB (NCBI) strong 48 99
o Dinoroseobacter shibae DFL-12 Dshi_1019 RNA modification enzyme, MiaB family (RefSeq) insig. 49 98
o Bosea sp. OAE506 ABIE41_RS04495 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 48 97
o Sinorhizobium meliloti 1021 SMc01115 (dimethylallyl)adenosine tRNA methylthiotransferase insig. 47 98
o Xanthobacter sp. DMC5 GFF3719 tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase sig. 47 100
o Caulobacter crescentus NA1000 CCNA_00054 tRNA 2-methylthioadenosine synthase strong 46 97
o Caulobacter crescentus NA1000 Δfur CCNA_00054 tRNA 2-methylthioadenosine synthase cofit 46 97
o Phaeobacter inhibens DSM 17395 PGA1_c04710 (dimethylallyl)adenosine tRNA methylthiotransferase MiaB sig. 47 98
o Brevundimonas sp. GW460-12-10-14-LB2 A4249_RS14720 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 45 98
o Rhodopseudomonas palustris CGA009 TX73_002325 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB insig. 44 98