| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_07895 | | class II fumarate hydratase | insig. | 100 | 100 |
| o | Pseudomonas sp. BP01 | JOY50_RS11090 | | lyase family protein | insig. | 68 | 99 |
| o | Pseudomonas putida KT2440 | PP_0944 | | fumarate hydratase (class 2) | insig. | 68 | 100 |
| o | Pseudomonas simiae WCS417 | PS417_04305 | | aspartate ammonia-lyase | insig. | 65 | 98 |
| o | Pseudomonas sp. SVBP6 | COO64_RS04245 | | lyase family protein | no data | 67 | 98 |
| o | Pseudomonas sp. RS175 | PFR28_00165 | | Fumarate hydratase class II | insig. | 65 | 98 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS04360 | | class II fumarate hydratase | insig. | 66 | 98 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS04360 | | class II fumarate hydratase | sig. | 66 | 98 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01067 | | Fumarate hydratase class II | insig. | 66 | 98 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_3276 | | Fumarate hydratase class II (EC 4.2.1.2) | insig. | 66 | 98 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_1088 | | Fumarate hydratase class II (EC 4.2.1.2) | no data | 65 | 98 |
| o | Pseudomonas aeruginosa MRSN321 | DY961_RS24125 | fumC;inference=COORDINATES | class II fumarate hydratase FumC | insig. | 65 | 99 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_01730 | | Fumarate hydratase class II 2 | sig. | 65 | 99 |
| o | Pseudomonas aeruginosa PUPa3 | DQ20_RS56585 | fumC;inference=COORDINATES | class II fumarate hydratase FumC | insig. | 65 | 99 |
| o | Pseudomonas sp. DMC3 | GFF1820 | | Fumarate hydratase class II | cofit | 65 | 100 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_5022 | | Fumarate hydratase class II (EC 4.2.1.2) | insig. | 66 | 98 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_07075 | | aspartate ammonia-lyase | insig. | 65 | 98 |
| o | Rhodanobacter sp. FW510-T8 | OKGIIK_06345 | aspA | aspartate ammonia-lyase | insig. | 65 | 99 |
| o | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS05855 | | class II fumarate hydratase | sig. | 66 | 98 |
| o | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS00885 | | class II fumarate hydratase | insig. | 65 | 99 |
| o | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS09230 | | class II fumarate hydratase | insig. | 65 | 99 |
| o | Rhodanobacter denitrificans MT42 | LRK55_RS00660 | | class II fumarate hydratase | insig. | 65 | 99 |
| o | Pseudomonas orientalis W4I3 | QF045_RS07295 | | class II fumarate hydratase | cofit | 65 | 98 |
| o | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.3403.1 | | Fumarate hydratase class II (EC 4.2.1.2) | insig. | 62 | 99 |
| o | Shewanella amazonensis SB2B | Sama_0822 | | fumarate hydratase (RefSeq) | insig. | 62 | 98 |
| o | Lysobacter sp. OAE881 | ABIE51_RS09015 | | class II fumarate hydratase | insig. | 63 | 98 |
| o | Shewanella loihica PV-4 | Shew_0068 | | fumarate lyase (RefSeq) | insig. | 60 | 99 |
| o | Marinobacter adhaerens HP15 | HP15_3132 | | fumarate hydratase class II | insig. | 58 | 99 |
| o | Alteromonas macleodii MIT1002 | MIT1002_03167 | | Fumarate hydratase class II | insig. | 59 | 98 |
| o | Kangiella aquimarina DSM 16071 | B158DRAFT_1200 | | fumarase, class II (EC 4.2.1.2) | insig. | 56 | 98 |
| o | Acinetobacter radioresistens SK82 | MPMX26_01446 | | Fumarate hydratase class II | insig. | 50 | 97 |
| o | Acinetobacter baumannii LAC-4 | RR41_RS07970 | | class II fumarate hydratase | insig. | 49 | 98 |
| o | Pseudomonas segetis P6 | ACVTMO_RS14195 | | class II fumarate hydratase | insig. | 48 | 99 |
| o | Paraburkholderia graminis OAS925 | ABIE53_003084 | | fumarate hydratase class II | strong | 49 | 93 |
| o | Escherichia fergusonii Becca | EFB2_02381 | | Fumarate hydratase class II | insig. | 46 | 97 |
| o | Escherichia coli ECRC100 | OKFHMN_27650 | fumC | class II fumarate hydratase | cofit | 46 | 97 |
| o | Escherichia coli ECRC101 | MCAODC_17760 | fumC | class II fumarate hydratase | insig. | 46 | 97 |
| o | Escherichia coli ECRC102 | NIAGMN_00425 | fumC | class II fumarate hydratase | insig. | 46 | 97 |
| o | Escherichia coli ECRC62 | BNILDI_16945 | fumC | class II fumarate hydratase | insig. | 46 | 97 |
| o | Escherichia coli ECRC98 | JDDGAC_02240 | fumC | class II fumarate hydratase | insig. | 46 | 97 |
| o | Escherichia coli ECRC99 | KEDOAH_00455 | fumC | class II fumarate hydratase | no data | 46 | 97 |
| o | Escherichia coli ECOR27 | NOLOHH_18155 | fumC | class II fumarate hydratase | insig. | 46 | 97 |
| o | Escherichia coli Nissle 1917 | ECOLIN_RS08995 | | class II fumarate hydratase | no data | 46 | 97 |
| o | Escherichia coli BL21 | ECD_01580 | | fumarate hydratase (fumarase C),aerobic Class II | no data | 46 | 97 |
| o | Escherichia coli BW25113 | b1611 | fumC | fumarate hydratase (NCBI) | strong | 46 | 97 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_04066 | | Fumarate hydratase class II | cofit | 51 | 97 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS18630 | | class II fumarate hydratase | insig. | 48 | 93 |
| o | Escherichia coli ECOR38 | HEPCGN_06025 | fumC | class II fumarate hydratase | insig. | 46 | 97 |
| o | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_02115 | | Fumarate hydratase class II | insig. | 46 | 97 |
| o | Erwinia amylovora T8 | OLJFJH_14300 | | class II fumarate hydratase | cofit | 45 | 97 |
| o | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF1806 | | Fumarate hydratase class II (EC 4.2.1.2) | insig. | 48 | 92 |
| o | Herbaspirillum seropedicae SmR1 | HSERO_RS07610 | | fumarate hydratase | insig. | 46 | 98 |
| o | Pseudomonas syringae pv. syringae B728a | Psyr_3661 | | Fumarate hydratase, class II | insig. | 48 | 98 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3661 | | Fumarate hydratase, class II | insig. | 48 | 98 |
| o | Synechocystis sp000284455 PCC 6803 | SGL_RS13465 | | class II fumarate hydratase | strong | 45 | 98 |
| | Pseudomonas simiae WCS417 | PS417_19200 | | fumarate hydratase | insig. | 49 | 97 |
| o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_04314 | | fumarase, class II | insig. | 49 | 93 |
| o | Klebsiella michiganensis M5al | BWI76_RS16270 | | fumarate hydratase, class II | sig. | 48 | 92 |
| o | Enterobacter asburiae PDN3 | EX28DRAFT_1608 | | fumarase, class II (EC 4.2.1.2) | strong | 46 | 97 |
| o | Enterobacter sp. TBS_079 | MPMX20_02022 | | Fumarate hydratase class II | insig. | 46 | 97 |
| o | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS05795 | | class II fumarate hydratase | cofit | 45 | 97 |
| o | Pectobacterium carotovorum WPP14 | HER17_RS10755 | | class II fumarate hydratase | cofit | 46 | 97 |
| o | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS17680 | | class II fumarate hydratase | no data | 46 | 97 |
| o | Ralstonia solanacearum UW163 | UW163_RS21470 | | class II fumarate hydratase | insig. | 46 | 97 |
| o | Mycobacterium tuberculosis H37Rv | Rv1098c | | Probable fumarase Fum (fumarate hydratase) | no data | 50 | 96 |
| o | Dickeya dadantii 3937 | DDA3937_RS10655 | | class II fumarate hydratase | sig. | 47 | 92 |
| o | Serratia liquefaciens MT49 | IAI46_11655 | | class II fumarate hydratase | strong | 48 | 92 |
| o | Rahnella sp. WP5 | EX31_RS06965 | | class II fumarate hydratase | insig. | 47 | 92 |
| o | Paraburkholderia sabiae LMG 24235 | QEN71_RS04360 | | class II fumarate hydratase | insig. | 48 | 90 |
| o | Dickeya dianthicola ME23 | DZA65_RS11125 | | class II fumarate hydratase | insig. | 47 | 92 |
| o | Pseudomonas sp. S08-1 | OH686_21015 | | fumarate hydratase, class II | insig. | 49 | 98 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS08220 | | class II fumarate hydratase | sig. | 48 | 98 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS44600 | | class II fumarate hydratase | sig. | 48 | 98 |
| o | Pantoea sp. MT58 | IAI47_10335 | | class II fumarate hydratase | strong | 45 | 97 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_03770 | | class II fumarate hydratase | insig. | 48 | 98 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_02150 | | fumarate hydratase | insig. | 48 | 98 |
| o | Dechlorosoma suillum PS | Dsui_0926 | | fumarate hydratase, class II | insig. | 47 | 97 |
| o | Dickeya dianthicola 67-19 | HGI48_RS10655 | | class II fumarate hydratase | insig. | 47 | 92 |
| | Pseudomonas orientalis W4I3 | QF045_RS21155 | | class II fumarate hydratase | insig. | 49 | 98 |
| o | Ralstonia solanacearum PSI07 | RPSI07_RS05900 | | class II fumarate hydratase | strong | 46 | 97 |
| o | Klebsiella pneumoniae MKP103 | KDGMDA_20940 | | class II fumarate hydratase | insig. | 45 | 98 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_03545 | | fumarate hydratase | no data | 49 | 98 |
| | Pseudomonas sp. DMC3 | GFF4693 | | Fumarate hydratase class II | cofit | 48 | 98 |
| o | Ralstonia solanacearum GMI1000 | RS_RS23320 | | class II fumarate hydratase | cofit | 45 | 97 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS03655 | | class II fumarate hydratase | insig. | 49 | 89 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS14615 | | fumarate hydratase | insig. | 48 | 90 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS21170 | | class II fumarate hydratase | insig. | 49 | 97 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS21170 | | class II fumarate hydratase | insig. | 49 | 97 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_367 | | Fumarate hydratase class II (EC 4.2.1.2) | sig. | 48 | 98 |
| o | Magnetospirillum magneticum AMB-1 | AMB_RS18480 | | class II fumarate hydratase | insig. | 46 | 97 |
| o | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_23250 | fumC | class II fumarate hydratase | cofit | 45 | 97 |
| o | Erwinia tracheiphila SCR3 | LU632_RS12335 | fumC | class II fumarate hydratase | insig. | 44 | 97 |
| | Pseudomonas sp. BP01 | JOY50_RS16210 | | class II fumarate hydratase | insig. | 48 | 98 |
| | Pseudomonas putida KT2440 | PP_1755 | | fumarate hydratase (class 2) | insig. | 48 | 98 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_2536 | | Fumarate hydratase class II (EC 4.2.1.2) | insig. | 48 | 98 |
| | Pseudomonas sp. RS175 | PFR28_00889 | | Fumarate hydratase class II | insig. | 48 | 98 |
| o | Pseudomonas stutzeri RCH2 | Psest_2682 | | fumarate hydratase, class II | strong | 47 | 98 |
| | Pseudomonas sp. SVBP6 | COO64_RS06820 | | class II fumarate hydratase | insig. | 48 | 98 |
| o | Variovorax sp. SCN45 | GFF479 | | Fumarate hydratase class II (EC 4.2.1.2) | insig. | 45 | 98 |
| o | Dinoroseobacter shibae DFL-12 | Dshi_2334 | | fumarate hydratase, class II (RefSeq) | no data | 43 | 97 |