| Organism | Ratio1 | Gene1 | Name1 | Description1 | Ratio2 | Gene2 | Name2 | Description2 | Cofit | Rank |
|---|
| Escherichia coli BW25113 | 1.0 | b2020 | hisD | histidinol dehydrogenase (NCBI) | 1.0 | b2025 | hisF | imidazole glycerol phosphate synthase subunit HisF (NCBI) | 0.96 | 8 |
| Escherichia coli ECOR27 | 0.99 | NOLOHH_15830 | hisD | Histidinol dehydrogenase | 0.99 | NOLOHH_15805 | hisF | Imidazole glycerol phosphate synthase subunit HisF | low | > 57 |
| Escherichia coli BL21 | 0.99 | ECD_01922 | | bifunctional histidinal dehydrogenase/ histidinol dehydrogenase | 0.99 | ECD_01927 | | imidazole glycerol phosphate synthase, catalytic subunit with HisH | — | — |
| Escherichia coli ECRC62 | 0.99 | BNILDI_14740 | hisD | Histidinol dehydrogenase | 1.00 | BNILDI_14715 | hisF | Imidazole glycerol phosphate synthase subunit HisF | — | — |
| Escherichia coli Nissle 1917 | 0.99 | ECOLIN_RS11945 | | histidinol dehydrogenase | 1.00 | ECOLIN_RS11970 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Escherichia coli ECOR38 | 0.99 | HEPCGN_03220 | hisD | Histidinol dehydrogenase | 0.99 | HEPCGN_03195 | hisF | Imidazole glycerol phosphate synthase subunit HisF | low | > 85 |
| Escherichia coli HS(pFamp)R (ATCC 700891) | 0.98 | OHPLBJKB_01680 | | Histidinol dehydrogenase | 0.99 | OHPLBJKB_01675 | | Imidazole glycerol phosphate synthase subunit HisF | low | > 73 |
| Escherichia fergusonii Becca | 0.98 | EFB2_01836 | | Histidinol dehydrogenase | 1.00 | EFB2_01831 | | Imidazole glycerol phosphate synthase subunit HisF | low | > 86 |
| Escherichia coli ECRC101 | 0.98 | MCAODC_20425 | hisD | Histidinol dehydrogenase | 1.00 | MCAODC_20450 | hisF | Imidazole glycerol phosphate synthase subunit HisF | — | — |
| Escherichia coli ECRC99 | 0.98 | KEDOAH_03635 | hisD | Histidinol dehydrogenase | 1.00 | KEDOAH_03660 | hisF | Imidazole glycerol phosphate synthase subunit HisF | — | — |
| Escherichia coli ECRC98 | 0.98 | JDDGAC_28625 | hisD | Histidinol dehydrogenase | 1.00 | JDDGAC_28600 | hisF | Imidazole glycerol phosphate synthase subunit HisF | — | — |
| Escherichia coli ECRC102 | 0.98 | NIAGMN_22460 | hisD | Histidinol dehydrogenase | 1.00 | NIAGMN_22435 | hisF | Imidazole glycerol phosphate synthase subunit HisF | — | — |
| Escherichia coli ECRC101 | 0.98 | OKFHMN_24515 | hisD | Histidinol dehydrogenase | 1.00 | OKFHMN_24490 | hisF | Imidazole glycerol phosphate synthase subunit HisF | — | — |
| Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | 0.93 | GFF3912 | | Histidinol dehydrogenase (EC 1.1.1.23) | 0.95 | GFF3907 | | Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-) | low | > 78 |
| Enterobacter asburiae PDN3 | 0.89 | EX28DRAFT_0539 | | histidinol dehydrogenase | 0.96 | EX28DRAFT_0534 | | imidazoleglycerol phosphate synthase, cyclase subunit | 0.91 | 12 |
| Enterobacter sp. TBS_079 | 0.89 | MPMX20_03002 | | Histidinol dehydrogenase | 0.97 | MPMX20_03007 | | Imidazole glycerol phosphate synthase subunit HisF | 0.97 | 5 |
| Klebsiella michiganensis M5al | 0.88 | BWI76_RS18925 | | histidinol dehydrogenase | 0.96 | BWI76_RS18950 | | imidazole glycerol phosphate synthase cyclase subunit | 0.90 | 6 |
| Serratia liquefaciens MT49 | 0.85 | IAI46_08210 | | histidinol dehydrogenase | 0.95 | IAI46_08185 | | imidazole glycerol phosphate synthase subunit HisF | 0.83 | 9 |
| Pantoea sp. MT58 | 0.82 | IAI47_07090 | | histidinol dehydrogenase | 0.90 | IAI47_07065 | | imidazole glycerol phosphate synthase subunit HisF | 0.98 | 4 |
| Erwinia tracheiphila SCR3 | 0.81 | LU632_RS08945 | hisD | histidinol dehydrogenase | 0.93 | LU632_RS08920 | hisF | imidazole glycerol phosphate synthase subunit HisF | 0.93 | 4 |
| Dickeya dadantii 3937 | 0.80 | DDA3937_RS09735 | | histidinol dehydrogenase | 0.93 | DDA3937_RS09710 | | imidazole glycerol phosphate synthase subunit HisF | 0.97 | 3 |
| Pectobacterium carotovorum WPP14 | 0.80 | HER17_RS12230 | | histidinol dehydrogenase | 0.91 | HER17_RS12255 | | imidazole glycerol phosphate synthase subunit HisF | 0.97 | 8 |
| Dickeya dianthicola 67-19 | 0.79 | HGI48_RS09745 | | histidinol dehydrogenase | 0.92 | HGI48_RS09720 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Rahnella sp. WP5 | 0.79 | EX31_RS11440 | | histidinol dehydrogenase | 0.91 | EX31_RS11465 | | imidazole glycerol phosphate synthase subunit HisF | 0.95 | 28 |
| Dickeya dianthicola ME23 | 0.79 | DZA65_RS10055 | | histidinol dehydrogenase | 0.92 | DZA65_RS10030 | | imidazole glycerol phosphate synthase subunit HisF | 0.84 | 14 |
| Vibrio cholerae E7946 ATCC 55056 | 0.63 | CSW01_05750 | | histidinol dehydrogenase | 0.87 | CSW01_05775 | | imidazole glycerol phosphate synthase cyclase subunit | 0.57 | 46 |
| Shewanella amazonensis SB2B | 0.59 | Sama_1942 | hisD | histidinol dehydrogenase (RefSeq) | 0.77 | Sama_1947 | | imidazole glycerol phosphate synthase subunit HisF (RefSeq) | 0.99 | 3 |
| Shewanella loihica PV-4 | 0.57 | Shew_2198 | | histidinol dehydrogenase (RefSeq) | 0.75 | Shew_2203 | | imidazole glycerol phosphate synthase subunit HisF (RefSeq) | 0.91 | 31 |
| Shewanella oneidensis MR-1 | 0.57 | SO2073 | hisD | histidinol dehydrogenase (NCBI ptt file) | 0.75 | SO2068 | hisF | hisF protein (cyclase) (NCBI ptt file) | 0.98 | 7 |
| Shewanella sp. ANA-3 | 0.56 | Shewana3_1853 | hisD | histidinol dehydrogenase (RefSeq) | 0.75 | Shewana3_1848 | | imidazole glycerol phosphate synthase subunit HisF (RefSeq) | 0.94 | 22 |
| Alteromonas macleodii MIT1002 | 0.54 | MIT1002_02633 | | Histidinol dehydrogenase | 0.76 | MIT1002_02638 | | Imidazole glycerol phosphate synthase subunit HisF | 0.97 | 26 |
| Rhodanobacter denitrificans FW104-10B01 | 0.53 | LRK54_RS01605 | | histidinol dehydrogenase | 0.65 | LRK54_RS01580 | | imidazole glycerol phosphate synthase subunit HisF | 0.58 | 48 |
| Rhodanobacter denitrificans MT42 | 0.53 | LRK55_RS01385 | | histidinol dehydrogenase | 0.64 | LRK55_RS01360 | | imidazole glycerol phosphate synthase subunit HisF | 0.59 | 41 |
| Dyella japonica UNC79MFTsu3.2 | 0.53 | ABZR86_RS06730 | | histidinol dehydrogenase | 0.63 | ABZR86_RS06705 | | imidazole glycerol phosphate synthase subunit HisF | 0.96 | 25 |
| Rhodanobacter sp. FW510-T8 | 0.53 | OKGIIK_04950 | hisD | histidinol dehydrogenase | 0.64 | OKGIIK_04975 | hisF | imidazole glycerol phosphate synthase subunit HisF | low | > 52 |
| Bacteroides ovatus ATCC 8483 | 0.53 | BACOVA_01234 | | histidinol dehydrogenase | 0.44 | BACOVA_00219 | | imidazoleglycerol phosphate synthase, cyclase subunit | 0.98 | 5 |
| Bacteroides thetaiotaomicron VPI-5482 | 0.52 | BT0201 | | histidinol dehydrogenase (NCBI ptt file) | 0.44 | BT1378 | | imidazole glycerol phosphate synthase subunit hisF (NCBI ptt file) | 0.87 | 31 |
| Phocaeicola vulgatus CL09T03C04 | 0.51 | HMPREF1058_RS15875 | | histidinol dehydrogenase | 0.46 | HMPREF1058_RS06175 | | imidazole glycerol phosphate synthase subunit HisF | 0.95 | 11 |
| Bacteroides stercoris CC31F | 0.50 | HMPREF1181_RS04860 | | histidinol dehydrogenase | 0.42 | HMPREF1181_RS11070 | | imidazole glycerol phosphate synthase subunit HisF | 0.97 | 4 |
| Phocaeicola dorei CL03T12C01 | 0.50 | ABI39_RS11835 | | histidinol dehydrogenase | 0.46 | ABI39_RS19930 | | imidazole glycerol phosphate synthase subunit HisF | 0.77 | 33 |
| Lysobacter sp. OAE881 | 0.49 | ABIE51_RS13325 | | histidinol dehydrogenase | 0.65 | ABIE51_RS13300 | | imidazole glycerol phosphate synthase subunit HisF | 0.90 | 54 |
| Parabacteroides merdae CL09T00C40 | 0.48 | HMPREF1078_RS02450 | | histidinol dehydrogenase | 0.43 | HMPREF1078_RS13565 | | imidazole glycerol phosphate synthase subunit HisF | 0.98 | 11 |
| Xanthomonas campestris pv. campestris strain 8004 | 0.48 | Xcc-8004.2948.1 | | Histidinol dehydrogenase (EC 1.1.1.23) | 0.63 | Xcc-8004.2943.1 | | Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-) | 0.88 | 11 |
| Echinicola vietnamensis KMM 6221, DSM 17526 | 0.47 | Echvi_2459 | | histidinol dehydrogenase | 0.41 | Echvi_2516 | | imidazoleglycerol phosphate synthase, cyclase subunit | 0.97 | 3 |
| Pontibacter actiniarum KMM 6156, DSM 19842 | 0.45 | CA264_05030 | | histidinol dehydrogenase | 0.47 | CA264_05110 | | imidazole glycerol phosphate synthase cyclase subunit | 0.43 | 11 |
| Mucilaginibacter yixingensis YX-36 DSM 26809 | 0.44 | ABZR88_RS06880 | | histidinol dehydrogenase | 0.43 | ABZR88_RS06855 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Brevundimonas sp. GW460-12-10-14-LB2 | 0.38 | A4249_RS10695 | | histidinol dehydrogenase | 0.57 | A4249_RS10715 | | imidazole glycerol phosphate synthase subunit HisF | 0.99 | 13 |
| Methanococcus maripaludis JJ | 0.38 | MMJJ_RS00120 | | histidinol dehydrogenase | 0.38 | MMJJ_RS05490 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Methanococcus maripaludis S2 | 0.37 | MMP_RS05015 | | histidinol dehydrogenase | 0.38 | MMP_RS08865 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Sinorhizobium meliloti 1021 | 0.35 | SMc02307 | | histidinol dehydrogenase | 0.36 | SMc02569 | | imidazole glycerol phosphate synthase subunit HisF | 0.94 | 5 |
| Cupriavidus basilensis FW507-4G11 | 0.35 | RR42_RS18915 | | histidinol dehydrogenase | 0.42 | RR42_RS18885 | | imidazole glycerol phosphate synthase | — | — |
| Rhizobium sp. OAE497 | 0.34 | ABIE40_RS02725 | | histidinol dehydrogenase | 0.36 | ABIE40_RS19440 | | imidazole glycerol phosphate synthase subunit HisF | 0.99 | 3 |
| Pseudomonas sp. S08-1 | 0.34 | OH686_08470 | | Histidinol dehydrogenase | 0.42 | OH686_14595 | | Imidazole glycerol phosphate synthase cyclase subunit | 0.73 | 37 |
| Agrobacterium fabrum C58 | 0.34 | Atu0537 | | histidinol dehydrogenase | 0.37 | Atu0039 | | imidazoleglycerol-phosphate synthase cyclase | 0.87 | 20 |
| Pseudomonas stutzeri RCH2 | 0.34 | Psest_3298 | | histidinol dehydrogenase | 0.42 | Psest_0156 | | imidazoleglycerol phosphate synthase, cyclase subunit | — | — |
| Magnetospirillum magneticum AMB-1 | 0.34 | AMB_RS16905 | | histidinol dehydrogenase | 0.37 | AMB_RS22925 | | imidazole glycerol phosphate synthase cyclase subunit | — | — |
| Dinoroseobacter shibae DFL-12 | 0.34 | Dshi_0979 | | Histidinol dehydrogenase (RefSeq) | 0.37 | Dshi_2576 | | imidazoleglycerol phosphate synthase, cyclase subunit (RefSeq) | — | — |
| Herbaspirillum seropedicae SmR1 | 0.33 | HSERO_RS20345 | | histidinol dehydrogenase | 0.39 | HSERO_RS20315 | | imidazole glycerol phosphate synthase | 0.89 | 9 |
| Phaeobacter inhibens DSM 17395 | 0.33 | PGA1_c31190 | | histidinol dehydrogenase HisD | 0.34 | PGA1_c09240 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Pseudomonas fluorescens SBW25 | 0.33 | PFLU_RS04470 | | histidinol dehydrogenase | 0.40 | PFLU_RS01625 | | imidazole glycerol phosphate synthase subunit HisF | low | > 109 |
| Pseudomonas simiae WCS417 | 0.33 | PS417_04420 | | histidinol dehydrogenase | 0.40 | PS417_01580 | | imidazole glycerol phosphate synthase | 0.83 | 26 |
| Pseudomonas fluorescens FW300-N1B4 | 0.33 | Pf1N1B4_1066 | | Histidinol dehydrogenase (EC 1.1.1.23) | 0.40 | Pf1N1B4_1623 | | Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-) | — | — |
| Pseudomonas fluorescens SBW25-INTG | 0.33 | PFLU_RS04470 | | histidinol dehydrogenase | 0.40 | PFLU_RS01625 | | imidazole glycerol phosphate synthase subunit HisF | 0.69 | 21 |
| Variovorax sp. OAS795 | 0.33 | ABID97_RS23160 | | histidinol dehydrogenase | 0.41 | ABID97_RS23105 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Variovorax sp. SCN45 | 0.33 | GFF5345 | | Histidinol dehydrogenase (EC 1.1.1.23) | 0.39 | GFF5357 | | Imidazole glycerol phosphate synthase cyclase subunit | — | — |
| Pseudomonas fluorescens FW300-N2C3 | 0.33 | AO356_06965 | | histidinol dehydrogenase | 0.40 | AO356_09795 | | imidazole glycerol phosphate synthase subunit HisF | 0.79 | 38 |
| Pseudomonas fluorescens FW300-N2E2 | 0.33 | Pf6N2E2_3252 | | Histidinol dehydrogenase (EC 1.1.1.23) | 0.40 | Pf6N2E2_3838 | | Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-) | — | — |
| Pseudomonas syringae pv. syringae B728a ΔmexB | 0.33 | Psyr_4133 | | histidinol dehydrogenase | 0.40 | Psyr_4893 | | imidazole glycerol phosphate synthase subunit hisF | — | — |
| Dechlorosoma suillum PS | 0.33 | Dsui_1411 | | histidinol dehydrogenase | 0.38 | Dsui_1418 | | imidazoleglycerol phosphate synthase, cyclase subunit | — | — |
| Marinobacter adhaerens HP15 | 0.33 | HP15_2428 | | histidinol dehydrogenase | 0.39 | HP15_3009 | | protein containing histidine biosynthesis, HisF domain | — | — |
| Pseudomonas syringae pv. syringae B728a | 0.33 | Psyr_4133 | | histidinol dehydrogenase | 0.40 | Psyr_4893 | | imidazole glycerol phosphate synthase subunit hisF | 0.83 | 22 |
| Pseudomonas fluorescens GW456-L13 | 0.33 | PfGW456L13_5000 | | Histidinol dehydrogenase (EC 1.1.1.23) | 0.40 | PfGW456L13_358 | | Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-) | 0.96 | 7 |
| Pseudomonas fluorescens FW300-N2E3 | 0.33 | AO353_05110 | | histidinol dehydrogenase | 0.40 | AO353_12090 | | imidazole glycerol phosphate synthase subunit HisF | 0.86 | 22 |
| Pseudomonas sp. RS175 | 0.33 | PFR28_00187 | | Histidinol dehydrogenase | 0.40 | PFR28_04933 | | Imidazole glycerol phosphate synthase subunit HisF | 0.66 | 21 |
| Synechococcus elongatus PCC 7942 | 0.33 | Synpcc7942_1519 | hisD | histidinol dehydrogenase | 0.37 | Synpcc7942_2087 | hisF | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Rhodopseudomonas palustris CGA009 | 0.32 | TX73_023540 | | histidinol dehydrogenase | 0.33 | TX73_001625 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Pseudomonas putida KT2440 | 0.32 | PP_0966 | | Histidinol dehydrogenase | 0.41 | PP_0293 | | Imidazole glycerol phosphate synthase subunit HisF | 0.85 | 27 |
| Ralstonia solanacearum PSI07 | 0.32 | RPSI07_RS10390 | | histidinol dehydrogenase | 0.42 | RPSI07_RS10420 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Acinetobacter radioresistens SK82 | 0.32 | MPMX26_02587 | | Histidinol dehydrogenase | 0.40 | MPMX26_02854 | | Imidazole glycerol phosphate synthase subunit HisF | 0.98 | 9 |
| Ralstonia sp. UNC404CL21Col | 0.32 | ABZR87_RS02500 | | histidinol dehydrogenase | 0.42 | ABZR87_RS02470 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Acidovorax sp. GW101-3H11 | 0.32 | Ac3H11_4724 | | Histidinol dehydrogenase (EC 1.1.1.23) | 0.34 | Ac3H11_4733 | | Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-) | — | — |
| Ralstonia solanacearum IBSBF1503 | 0.32 | RALBFv3_RS06845 | | histidinol dehydrogenase | 0.43 | RALBFv3_RS06815 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Desulfovibrio vulgaris Hildenborough JW710 | 0.32 | DVU0796 | hisD | histidinol dehydrogenase (TIGR) | 0.37 | DVU0286 | hisF | imidazoleglycerol phosphate synthase, cyclase subunit (TIGR) | 0.93 | 3 |
| Ralstonia solanacearum UW163 | 0.32 | UW163_RS06770 | | histidinol dehydrogenase | 0.43 | UW163_RS06800 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Ralstonia solanacearum GMI1000 | 0.32 | RS_RS14790 | | histidinol dehydrogenase | 0.42 | RS_RS14760 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Azospirillum sp. SherDot2 | 0.32 | MPMX19_05543 | | Histidinol dehydrogenase | 0.40 | MPMX19_02542 | | Imidazole glycerol phosphate synthase subunit HisF | — | — |
| Bosea sp. OAE506 | 0.32 | ABIE41_RS24015 | | histidinol dehydrogenase | 0.34 | ABIE41_RS03810 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Sphingomonas koreensis DSMZ 15582 | 0.31 | Ga0059261_1281 | | histidinol dehydrogenase | 0.35 | Ga0059261_1048 | | imidazoleglycerol phosphate synthase, cyclase subunit | 0.99 | 2 |
| Rhodospirillum rubrum S1H | 0.31 | Rru_A2771 | | Histidinol dehydrogenase (NCBI) | 0.36 | Rru_A3594 | | Histidine biosynthesis protein HisF (NCBI) | low | > 58 |
| Burkholderia phytofirmans PsJN | 0.31 | BPHYT_RS17710 | | histidinol dehydrogenase | 0.41 | BPHYT_RS17680 | | imidazole glycerol phosphate synthase subunit HisF | — | — |
| Paraburkholderia graminis OAS925 | 0.31 | ABIE53_003701 | | histidinol dehydrogenase | 0.41 | ABIE53_003695 | | cyclase | — | — |
| Paraburkholderia sabiae LMG 24235 | 0.31 | QEN71_RS27865 | | histidinol dehydrogenase | 0.40 | QEN71_RS27835 | | imidazole glycerol phosphate synthase subunit HisF | 0.96 | 2 |
| Hydrogenophaga sp. GW460-11-11-14-LB1 | 0.31 | GFF1314 | | Histidinol dehydrogenase (EC 1.1.1.23) | 0.38 | GFF1309 | | Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-) | — | — |
| Castellaniella sp019104865 MT123 | 0.31 | ABCV34_RS11230 | | histidinol dehydrogenase | 0.37 | ABCV34_RS11250 | | imidazole glycerol phosphate synthase subunit HisF | 0.85 | 47 |
| Paraburkholderia bryophila 376MFSha3.1 | 0.31 | H281DRAFT_05652 | | histidinol dehydrogenase | 0.41 | H281DRAFT_05659 | | imidazole glycerol phosphate synthase subunit hisF | — | — |
| Desulfovibrio vulgaris Miyazaki F | 0.30 | DvMF_2814 | hisD | histidinol dehydrogenase (RefSeq) | 0.36 | DvMF_2304 | | imidazole glycerol phosphate synthase subunit HisF (RefSeq) | 0.95 | 9 |
| Bifidobacterium breve UCC2003 | 0.28 | BBR_RS17055 | | histidinol dehydrogenase | 0.40 | BBR_RS16095 | | imidazole glycerol phosphate synthase subunit HisF | 0.80 | 3 |
| Caulobacter crescentus NA1000 Δfur | 0.27 | CCNA_02431 | | histidinol dehydrogenase | 0.41 | CCNA_03853 | | imidazole glycerol phosphate synthase, cyclase subunit | low | > 67 |
| Caulobacter crescentus NA1000 | 0.27 | CCNA_02431 | | histidinol dehydrogenase | 0.41 | CCNA_03853 | | imidazole glycerol phosphate synthase, cyclase subunit | 0.99 | 1 |
| Mycobacterium tuberculosis H37Rv | 0.27 | Rv1599 | | Probable histidinol dehydrogenase HisD (HDH) | 0.37 | Rv1605 | | Probable cyclase HisF | — | — |
| Kangiella aquimarina DSM 16071 | 0.25 | B158DRAFT_2293 | | histidinol dehydrogenase | 0.39 | B158DRAFT_2291 | | imidazoleglycerol phosphate synthase, cyclase subunit | low | > 40 |
Not shown: 1 genomes with orthologs for b2020 only; 1 genomes with orthologs for b2025 only