| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Pseudomonas syringae pv. syringae B728a | Psyr_0033 | | tryptophan synthase, alpha chain | strong | 100 | 100 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_0033 | | tryptophan synthase, alpha chain | strong | 100 | 100 |
| o | Pseudomonas sp. BP01 | JOY50_RS21315 | trpA | tryptophan synthase subunit alpha | strong | 89 | 100 |
| o | Pseudomonas putida KT2440 | PP_0082 | | tryptophan synthase alpha chain | strong | 89 | 100 |
| o | Pseudomonas sp. SVBP6 | COO64_RS02270 | trpA | tryptophan synthase subunit alpha | strong | 87 | 100 |
| o | Pseudomonas simiae WCS417 | PS417_00180 | | tryptophan synthase subunit alpha | strong | 79 | 100 |
| o | Pseudomonas sp. DMC3 | GFF677 | | Tryptophan synthase alpha chain | strong | 79 | 100 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS00175 | | tryptophan synthase subunit alpha | strong | 78 | 100 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS00175 | | tryptophan synthase subunit alpha | strong | 78 | 100 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_565 | | Tryptophan synthase alpha chain (EC 4.2.1.20) | strong | 79 | 100 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_00241 | | Tryptophan synthase alpha chain | strong | 78 | 100 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_10705 | | tryptophan synthase subunit alpha | strong | 78 | 100 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_10910 | | tryptophan synthase subunit alpha | strong | 78 | 100 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_4047 | | Tryptophan synthase alpha chain (EC 4.2.1.20) | strong | 78 | 100 |
| o | Pseudomonas sp. RS175 | PFR28_04716 | | Tryptophan synthase alpha chain | no data | 77 | 100 |
| o | Pseudomonas sp. S08-1 | OH686_12430 | | tryptophan synthase, alpha subunit | strong | 77 | 100 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_1830 | | Tryptophan synthase alpha chain (EC 4.2.1.20) | strong | 76 | 100 |
| o | Pseudomonas segetis P6 | MPMX49_00374 | | Tryptophan synthase alpha chain | no data | 75 | 100 |
| o | Pseudomonas stutzeri RCH2 | Psest_0062 | | tryptophan synthase, alpha subunit | strong | 76 | 100 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_25380 | | tryptophan synthase subunit alpha | insig. | 75 | 100 |
| o | Bosea sp. OAE506 | ABIE41_RS03930 | | tryptophan synthase subunit alpha | no data | 60 | 100 |
| o | Phaeobacter inhibens DSM 17395 | PGA1_c04930 | | tryptophan synthase alpha chain | no data | 59 | 99 |
| o | Rhodopseudomonas palustris CGA009 | TX73_000365 | | tryptophan synthase subunit alpha | no data | 60 | 100 |
| o | Xanthobacter sp. DMC5 | GFF3884 | | Tryptophan synthase alpha chain | no data | 61 | 100 |
| o | Magnetospirillum magneticum AMB-1 | AMB_RS20300 | | tryptophan synthase subunit alpha | no data | 62 | 100 |
| o | Dinoroseobacter shibae DFL-12 | Dshi_0952 | | tryptophan synthase, alpha subunit (RefSeq) | no data | 58 | 99 |
| o | Azospirillum sp. SherDot2 | MPMX19_02737 | | Tryptophan synthase alpha chain | strong | 62 | 99 |
| o | Rhizobium sp. OAE497 | ABIE40_RS19340 | | tryptophan synthase subunit alpha | no data | 56 | 99 |
| o | Sinorhizobium meliloti 1021 | SMc02765 | | tryptophan synthase subunit alpha | strong | 58 | 99 |
| o | Azospirillum brasilense Sp245 | AZOBR_RS00520 | | tryptophan synthase subunit alpha | strong | 62 | 99 |
| o | Agrobacterium fabrum C58 | Atu0019 | | tryptophan synthase alpha subunit | no data | 55 | 99 |
| o | Rhodospirillum rubrum S1H | Rru_A3428 | | Tryptophan synthase (NCBI) | strong | 61 | 89 |
| o | Caulobacter crescentus NA1000 | CCNA_03657 | | tryptophan synthase alpha chain | strong | 59 | 99 |
| o | Caulobacter crescentus NA1000 Δfur | CCNA_03657 | | tryptophan synthase alpha chain | cofit | 59 | 99 |
| o | Sphingobium sp. HT1-2 | GFF1627 | | Tryptophan synthase alpha chain (EC 4.2.1.20) | strong | 56 | 89 |
| o | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS11320 | | tryptophan synthase subunit alpha | no data | 52 | 99 |
| o | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0239 | | tryptophan synthase, alpha chain (EC 4.2.1.20) | cofit | 57 | 89 |
| o | Dechlorosoma suillum PS | Dsui_3191 | | tryptophan synthase, alpha subunit | no data | 49 | 100 |
| o | Marinobacter adhaerens HP15 | HP15_1826 | | tryptophan synthase, alpha subunit | no data | 49 | 95 |
| o | Herbaspirillum seropedicae SmR1 | HSERO_RS16470 | | tryptophan synthase subunit alpha | strong | 50 | 94 |
| o | Acinetobacter radioresistens SK82 | MPMX26_02611 | | Tryptophan synthase alpha chain | no data | 48 | 98 |
| o | Acidovorax sp. GW101-3H11 | Ac3H11_1535 | | Tryptophan synthase alpha chain (EC 4.2.1.20) | strong | 47 | 99 |
| o | Ralstonia solanacearum PSI07 | RPSI07_RS14740 | | tryptophan synthase subunit alpha | no data | 49 | 90 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS14490 | | tryptophan synthase subunit alpha | strong | 49 | 90 |
| o | Acinetobacter baumannii LAC-4 | RR41_RS03140 | | tryptophan synthase subunit alpha | cofit | 47 | 98 |
| o | Ralstonia solanacearum GMI1000 | RS_RS09955 | | tryptophan synthase subunit alpha | cofit | 48 | 93 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF3354 | | Tryptophan synthase alpha chain (EC 4.2.1.20) | strong | 49 | 88 |
| o | Paraburkholderia graminis OAS925 | ABIE53_004811 | | tryptophan synthase alpha chain | strong | 45 | 99 |
| o | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS02575 | | tryptophan synthase subunit alpha | no data | 48 | 90 |
| o | Ralstonia solanacearum UW163 | UW163_RS11150 | | tryptophan synthase subunit alpha | no data | 48 | 90 |
| o | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS07995 | | tryptophan synthase subunit alpha | strong | 47 | 93 |
| o | Variovorax sp. SCN45 | GFF3882 | | Tryptophan synthase alpha chain (EC 4.2.1.20) | no data | 47 | 89 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS14350 | | tryptophan synthase alpha chain | no data | 47 | 90 |
| o | Variovorax sp. OAS795 | ABID97_RS08425 | | tryptophan synthase subunit alpha | strong | 48 | 89 |
| o | Lysobacter sp. OAE881 | ABIE51_RS08265 | | tryptophan synthase subunit alpha | strong | 47 | 91 |
| o | Rhodanobacter sp. FW510-T8 | OKGIIK_04850 | trpA | tryptophan synthase subunit alpha | strong | 46 | 93 |
| o | Rhodanobacter denitrificans MT42 | LRK55_RS01460 | | tryptophan synthase subunit alpha | strong | 47 | 90 |
| o | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS01680 | | tryptophan synthase, alpha subunit (EC 4.2.1.20) (from data) | strong | 47 | 90 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS33865 | | tryptophan synthase alpha chain | strong | 48 | 88 |
| o | Paraburkholderia sabiae LMG 24235 | QEN71_RS17655 | | tryptophan synthase subunit alpha | strong | 47 | 93 |
| o | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS08355 | | tryptophan synthase subunit alpha | insig. | 47 | 90 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS08430 | | tryptophan synthase subunit alpha | no data | 47 | 90 |
| o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06065 | | tryptophan synthase, alpha chain | strong | 44 | 99 |
| o | Methylophilus sp. DMC18 | GFF657 | | Tryptophan synthase alpha chain | no data | 47 | 80 |
| o | Synechococcus elongatus PCC 7942 | Synpcc7942_0411 | trpA | tryptophan synthase subunit alpha | no data | 43 | 98 |
| o | Synechocystis sp000284455 PCC 6803 | SGL_RS03395 | | tryptophan synthase subunit alpha | no data | 42 | 98 |
| o | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.1967.1 | | Tryptophan synthase alpha chain (EC 4.2.1.20) | strong | 46 | 90 |
| o | Methanococcus maripaludis JJ | MMJJ_RS09195 | | tryptophan synthase subunit alpha | no data | 41 | 93 |
| o | Methanococcus maripaludis S2 | MMP_RS05185 | | tryptophan synthase subunit alpha | no data | 40 | 93 |
| | Pseudomonas sp. BP01 | JOY50_RS13095 | trpA | tryptophan synthase subunit alpha | insig. | 38 | 90 |
| o | Desulfovibrio vulgaris Miyazaki F | DvMF_1740 | trpA | tryptophan synthase subunit alpha (RefSeq) | cofit | 43 | 92 |
| o | Desulfovibrio vulgaris Hildenborough JW710 | DVU0471 | trpA | tryptophan synthase, alpha subunit (TIGR) | strong | 41 | 88 |
| o | Shewanella oneidensis MR-1 | SO3024 | trpA | tryptophan synthase, alpha subunit (NCBI ptt file) | strong | 39 | 99 |
| o | Shewanella sp. ANA-3 | Shewana3_1515 | trpA | tryptophan synthase subunit alpha (RefSeq) | strong | 37 | 99 |
| o | Escherichia coli ECRC100 | OKFHMN_02675 | trpA | tryptophan synthase subunit alpha | cofit | 40 | 81 |
| o | Escherichia coli ECRC101 | MCAODC_15590 | trpA | tryptophan synthase subunit alpha | strong | 40 | 81 |
| o | Escherichia coli ECRC102 | NIAGMN_26845 | trpA | tryptophan synthase subunit alpha | cofit | 40 | 81 |
| o | Escherichia coli ECRC98 | JDDGAC_05220 | trpA | tryptophan synthase subunit alpha | strong | 40 | 81 |
| o | Escherichia coli ECRC99 | KEDOAH_26075 | trpA | tryptophan synthase subunit alpha | no data | 40 | 81 |
| o | Escherichia coli BL21 | ECD_01234 | | tryptophan synthase, alpha subunit | cofit | 40 | 81 |
| o | Escherichia coli ECOR27 | NOLOHH_20335 | trpA | tryptophan synthase subunit alpha | strong | 40 | 81 |
| o | Escherichia coli ECRC62 | BNILDI_18480 | trpA | tryptophan synthase subunit alpha | strong | 40 | 81 |
| o | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_02423 | | Tryptophan synthase alpha chain | insig. | 40 | 81 |
| o | Escherichia coli BW25113 | b1260 | trpA | tryptophan synthase subunit alpha (NCBI) | strong | 40 | 81 |
| o | Escherichia coli Nissle 1917 | ECOLIN_RS07595 | | tryptophan synthase subunit alpha | no data | 39 | 81 |
| o | Shewanella loihica PV-4 | Shew_2256 | trpA | tryptophan synthase subunit alpha (RefSeq) | strong | 40 | 93 |
| o | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF2726 | | Tryptophan synthase alpha chain (EC 4.2.1.20) | insig. | 37 | 99 |
| o | Alteromonas macleodii MIT1002 | MIT1002_01425 | | Tryptophan synthase alpha chain | no data | 35 | 99 |
| o | Shewanella amazonensis SB2B | Sama_2134 | trpA | tryptophan synthase subunit alpha (RefSeq) | strong | 40 | 81 |
| o | Enterobacter asburiae PDN3 | EX28DRAFT_0932 | | tryptophan synthase, alpha chain (EC 4.2.1.20) | strong | 35 | 99 |
| o | Enterobacter sp. TBS_079 | MPMX20_02514 | | Tryptophan synthase alpha chain | strong | 35 | 99 |
| o | Escherichia coli ECOR38 | HEPCGN_25515 | trpA | tryptophan synthase subunit alpha | strong | 39 | 81 |
| o | Escherichia fergusonii Becca | EFB2_02666 | | Tryptophan synthase alpha chain | insig. | 38 | 81 |
| o | Bacteroides ovatus ATCC 8483 | BACOVA_03785 | | tryptophan synthase, alpha subunit | strong | 36 | 93 |
| o | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_2511 | | tryptophan synthase, alpha subunit | no data | 34 | 91 |
| o | Klebsiella pneumoniae MKP103 | KDGMDA_19545 | | tryptophan synthase subunit alpha | insig. | 40 | 81 |
| o | Parabacteroides merdae CL09T00C40 | HMPREF1078_RS00120 | | tryptophan synthase subunit alpha | strong | 37 | 90 |
| o | Bacteroides thetaiotaomicron VPI-5482 | BT0527 | | tryptophan synthase alpha chain (NCBI ptt file) | strong | 35 | 93 |
| o | Klebsiella michiganensis M5al | BWI76_RS11840 | | tryptophan synthase subunit alpha | strong | 40 | 81 |
| o | Mucilaginibacter yixingensis YX-36 DSM 26809 | ABZR88_RS10660 | | tryptophan synthase subunit alpha | no data | 34 | 91 |