| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Pseudomonas simiae WCS417 | PS417_03095 | | ATPase | sig. | 100 | 100 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS03175 | | CpaF family protein | insig. | 99 | 100 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS03175 | | CpaF family protein | strong | 99 | 100 |
| o | Pseudomonas orientalis W4I3 | QF045_RS06130 | | CpaF family protein | cofit | 93 | 99 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_1278 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 90 | 100 |
| o | Pseudomonas sp. DMC3 | GFF3341 | | hypothetical protein | cofit | 90 | 100 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_13820 | | ATPase | insig. | 89 | 100 |
| o | Pseudomonas syringae pv. syringae B728a | Psyr_4393 | | type II secretion system protein E | sig. | 87 | 98 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_4393 | | type II secretion system protein E | sig. | 87 | 98 |
| o | Pseudomonas sp. SVBP6 | COO64_RS18465 | | CpaF family protein | insig. | 83 | 99 |
| o | Pseudomonas segetis P6 | ACVTMO_RS20025 | | CpaF family protein | insig. | 77 | 98 |
| o | Pseudomonas aeruginosa MRSN321 | DY961_RS24995 | tadA;inference=COORDINATES | type 4b pilus Flp biogenesis ATPase TadA | insig. | 72 | 97 |
| o | Pseudomonas aeruginosa PUPa3 | DQ20_RS57450 | tadA;inference=COORDINATES | type 4b pilus Flp biogenesis ATPase TadA | sig. | 72 | 97 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_02600 | | type 4b pilus Flp biogenesis ATPase TadA | sig. | 72 | 97 |
| o | Marinobacter adhaerens HP15 | HP15_p187g9 | | type II secretion system protein E | strong | 53 | 89 |
| o | Ralstonia solanacearum PSI07 | RPSI07_RS05045 | | CpaF family protein | insig. | 49 | 94 |
| o | Ralstonia solanacearum GMI1000 | RS_RS22355 | | CpaF family protein | insig. | 49 | 94 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS10645 | | CpaF family protein | insig. | 49 | 93 |
| o | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS18510 | | CpaF family protein | insig. | 48 | 94 |
| o | Ralstonia solanacearum UW163 | UW163_RS20620 | | CpaF family protein | insig. | 48 | 94 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS19545 | | CpaF family protein | strong | 48 | 93 |
| | Variovorax sp. OAS795 | ABID97_RS24290 | | CpaF family protein | no data | 50 | 88 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4121 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | no data | 49 | 89 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS08185 | | pilus assembly protein CpaF | insig. | 48 | 94 |
| | Variovorax sp. SCN45 | GFF2208 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 50 | 88 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS22920 | | pilus assembly protein CpaF | insig. | 49 | 89 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS35985 | | pilus assembly protein CpaF | sig. | 49 | 90 |
| o | Paraburkholderia graminis OAS925 | ABIE53_002438 | | pilus assembly protein CpaF | cofit | 48 | 94 |
| | Ralstonia solanacearum GMI1000 | RS_RS03280 | | CpaF family protein | insig. | 51 | 88 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS04100 | | pilus assembly protein CpaF | insig. | 49 | 90 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06368 | | pilus assembly protein CpaF | no data | 50 | 90 |
| | Paraburkholderia graminis OAS925 | ABIE53_004998 | | pilus assembly protein CpaF | sig. | 48 | 94 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS08500 | | CpaF family protein | insig. | 51 | 88 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS08110 | | CpaF family protein | insig. | 52 | 80 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS12510 | | CpaF family protein | insig. | 48 | 91 |
| | Ralstonia solanacearum UW163 | UW163_RS01055 | | CpaF family protein | insig. | 48 | 91 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2738 | | type II secretion system protein E (RefSeq) | insig. | 53 | 78 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS12005 | | CpaF family protein | insig. | 49 | 83 |
| | Rhodopseudomonas palustris CGA009 | TX73_019075 | | CpaF family protein | insig. | 48 | 88 |
| | Caulobacter crescentus NA1000 | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 51 | 80 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 51 | 80 |
| | Shewanella loihica PV-4 | Shew_0644 | | FHA domain-containing protein (RefSeq) | insig. | 51 | 78 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_496 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 50 | 89 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_01570 | | pilus assembly protein CpaF | insig. | 49 | 91 |
| | Agrobacterium fabrum C58 | Atu0218 | | component of type IV pilus | insig. | 48 | 87 |
| | Rhizobium sp. OAE497 | ABIE40_RS00885 | | CpaF family protein | insig. | 47 | 88 |
| | Bosea sp. OAE506 | ABIE41_RS03685 | | CpaF family protein | insig. | 47 | 88 |
| | Sinorhizobium meliloti 1021 | SMc02820 | | pilus assembly protein | insig. | 47 | 91 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS18950 | | CpaF family protein | insig. | 45 | 91 |
| | Pseudomonas sp. RS175 | PFR28_03044 | | hypothetical protein | insig. | 48 | 92 |
| | Pseudomonas sp. SVBP6 | COO64_RS23430 | | CpaF family protein | insig. | 51 | 89 |
| | Shewanella amazonensis SB2B | Sama_0708 | | type II/IV secretion system protein E (RefSeq) | insig. | 49 | 77 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_19980 | | pilus assembly protein CpaF | insig. | 48 | 92 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS20760 | | CpaF family protein | insig. | 49 | 91 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5915 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 52 | 82 |
| | Variovorax sp. OAS795 | ABID97_RS09105 | | CpaF family protein | insig. | 45 | 93 |
| | Pseudomonas sp. S08-1 | OH686_19900 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 50 | 84 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c06110 | | Flp pilus assembly protein, ATPase CpaF | insig. | 50 | 80 |
| | Pectobacterium carotovorum WPP14 | HER17_RS17705 | | CpaF family protein | insig. | 47 | 92 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS34040 | | hypothetical protein | insig. | 50 | 78 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2070 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 49 | 88 |
| | Sinorhizobium meliloti 1021 | SMa1568 | | CpaF2 pilus assembly protein | insig. | 48 | 80 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS17490 | | ATPase, T2SS/T4P/T4SS family | insig. | 45 | 91 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2122 | cpaF | type II/IV secretion system protein (TIGR) | insig. | 53 | 77 |
| | Pseudomonas sp. S08-1 | OH686_02520 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 54 | 81 |
| | Dinoroseobacter shibae DFL-12 | Dshi_1126 | | type II secretion system protein E (RefSeq) | insig. | 47 | 90 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS04365 | | ATPase, T2SS/T4P/T4SS family | insig. | 52 | 77 |
| | Pseudomonas stutzeri RCH2 | Psest_2619 | | Flp pilus assembly protein, ATPase CpaF | insig. | 53 | 81 |
| | Sphingobium sp. HT1-2 | GFF4554 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | cofit | 46 | 83 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0975 | | Flp pilus assembly protein, ATPase CpaF | insig. | 42 | 91 |
| | Mycobacterium tuberculosis H37Rv | Rv3659c | | Conserved hypothetical protein | insig. | 42 | 69 |
| | Methanococcus maripaludis JJ | MMJJ_RS05235 | | Flp pilus assembly complex ATPase component TadA | insig. | 38 | 84 |
| | Methanococcus maripaludis S2 | MMP_RS00250 | | Flp pilus assembly complex ATPase component TadA | strong | 38 | 84 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0389 | | P-type conjugative transfer ATPase TrbB | insig. | 40 | 71 |
| | Caulobacter crescentus NA1000 | CCNA_02505 | | type IV secretory pathway, VirB11 ATPase | sig. | 35 | 68 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_02505 | | type IV secretory pathway, VirB11 ATPase | cofit | 35 | 68 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2912 | | P-type conjugative transfer ATPase TrbB | insig. | 37 | 71 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_3072 | | P-type conjugative transfer ATPase TrbB | insig. | 37 | 71 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2572 | | P-type conjugative transfer ATPase TrbB | insig. | 37 | 68 |
| | Rhodopseudomonas palustris CGA009 | TX73_021370 | | P-type conjugative transfer ATPase TrbB | insig. | 35 | 78 |
| | Sphingobium sp. HT1-2 | GFF188 | | Conjugative transfer protein TrbB | cofit | 35 | 71 |
| | Marinobacter adhaerens HP15 | HP15_p42g52 | | type II secretion system protein E | cofit | 33 | 70 |
| | Xanthobacter sp. DMC5 | GFF3638 | | hypothetical protein | insig. | 34 | 71 |
| | Agrobacterium fabrum C58 | Atu6041 | | conjugal transfer protein | insig. | 32 | 72 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS01870 | | P-type DNA transfer ATPase VirB11 | insig. | 36 | 66 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS43755 | | ATPase, T2SS/T4P/T4SS family | insig. | 32 | 73 |
| | Xanthobacter sp. DMC5 | GFF2635 | | hypothetical protein | insig. | 34 | 71 |
| | Bifidobacterium breve UCC2003 | BBR_RS10950 | | pilus biosynthesis protein | insig. | 37 | 68 |
| | Rhodopseudomonas palustris CGA009 | TX73_011525 | | P-type conjugative transfer ATPase TrbB | insig. | 36 | 68 |
| | Rhodospirillum rubrum S1H | Rru_A0808 | | type II secretion system protein E (NCBI) | insig. | 36 | 60 |
| | Rhodopseudomonas palustris CGA009 | TX73_021310 | | P-type conjugative transfer ATPase TrbB | insig. | 38 | 60 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4435 | | Conjugative transfer protein TrbB | insig. | 33 | 70 |
| | Sinorhizobium meliloti 1021 | SMa1302 | | VirB11-like ATPase | insig. | 33 | 70 |
| | Dechlorosoma suillum PS | Dsui_2579 | | Flp pilus assembly protein, ATPase CpaF | cofit | 32 | 77 |
| | Methanococcus maripaludis JJ | MMJJ_RS05745 | | type II/IV secretion system ATPase subunit | cofit | 30 | 78 |
| | Methanococcus maripaludis S2 | MMP_RS08625 | | type II/IV secretion system ATPase subunit | cofit | 30 | 78 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS00155 | | P-type conjugative transfer ATPase TrbB | insig. | 34 | 71 |
| | Variovorax sp. SCN45 | GFF4849 | | Conjugative transfer protein TrbB | insig. | 35 | 71 |
| | Dickeya dadantii 3937 | DDA3937_RS03995 | | P-type conjugative transfer ATPase TrbB | insig. | 35 | 73 |
| | Variovorax sp. SCN45 | GFF6579 | | Conjugative transfer protein TrbB | insig. | 33 | 71 |
| | Agrobacterium fabrum C58 | Atu5172 | | type IV secretion protein AvhB11 | insig. | 32 | 69 |
| | Shewanella sp. ANA-3 | Shewana3_1282 | | type II secretion system protein E (RefSeq) | cofit | 33 | 71 |
| | Dickeya dianthicola 67-19 | HGI48_RS03925 | | P-type conjugative transfer ATPase TrbB | insig. | 35 | 70 |
| | Dickeya dianthicola 67-19 | HGI48_RS08420 | | P-type conjugative transfer ATPase TrbB | insig. | 34 | 73 |
| | Agrobacterium fabrum C58 | Atu6177 | | component of type IV secretion system | insig. | 32 | 64 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11415 | | P-type DNA transfer ATPase VirB11 | insig. | 28 | 67 |
| | Ralstonia solanacearum GMI1000 | RS_RS12955 | | P-type conjugative transfer ATPase TrbB | insig. | 33 | 70 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_12825 | | P-type conjugative transfer ATPase TrbB | no data | 32 | 70 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4176 | | Conjugative transfer protein TrbB | insig. | 32 | 70 |
| | Variovorax sp. SCN45 | GFF4414 | | Conjugative transfer protein TrbB | insig. | 32 | 70 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF3453 | | Conjugative transfer protein TrbB | insig. | 34 | 66 |
| | Acinetobacter radioresistens SK82 | MPMX26_03199 | | Type IV secretion system protein VirB11 | insig. | 29 | 65 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS32155 | | type IV secretion system protein VirB11 | sig. | 31 | 66 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS06740 | | P-type DNA transfer ATPase VirB11 | insig. | 31 | 66 |
| | Escherichia coli ECOR38 | HEPCGN_00330 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 28 | 65 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS03320 | | P-type DNA transfer ATPase VirB11 | insig. | 31 | 65 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.2046.1 | | hypothetical protein | insig. | 27 | 71 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c22920 | | putative type IV secretion system protein VirB11 | insig. | 31 | 66 |
| | Ralstonia solanacearum UW163 | UW163_RS23875 | | P-type DNA transfer ATPase VirB11 | insig. | 32 | 58 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS00810 | | type IV secretion system protein VirB11 | insig. | 29 | 65 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3983 | | type II secretion system protein E (RefSeq) | insig. | 36 | 50 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3648 | | type II secretion system protein E (RefSeq) | insig. | 35 | 50 |
| | Dickeya dadantii 3937 | DDA3937_RS13875 | | P-type DNA transfer ATPase VirB11 | insig. | 28 | 64 |
| | Escherichia coli ECOR27 | NOLOHH_00295 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 27 | 68 |
| | Dickeya dianthicola ME23 | DZA65_RS14430 | | P-type DNA transfer ATPase VirB11 | insig. | 30 | 55 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS40910 | | P-type DNA transfer ATPase VirB11 | no data | 28 | 63 |
| | Escherichia coli ECOR27 | NOLOHH_00210 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 27 | 65 |
| | Dickeya dianthicola 67-19 | HGI48_RS07090 | | P-type DNA transfer ATPase VirB11 | insig. | 28 | 64 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS36345 | | hypothetical protein | insig. | 31 | 37 |
| | Azospirillum brasilense Sp245 | AZOBR_RS33110 | | hypothetical protein | insig. | 27 | 55 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS30925 | | GspE/PulE family protein | insig. | 37 | 35 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS04100 | | type II/IV secretion system protein | insig. | 38 | 32 |
| | Ralstonia solanacearum UW163 | UW163_RS09570 | | type II/IV secretion system protein | insig. | 38 | 32 |
| | Ralstonia solanacearum GMI1000 | RS_RS11580 | | type II/IV secretion system protein | insig. | 37 | 33 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_02465 | | twitching motility protein PilT | insig. | 36 | 30 |
| | Alteromonas macleodii MIT1002 | MIT1002_03059 | | Twitching mobility protein | insig. | 32 | 50 |
| | Pseudomonas orientalis W4I3 | QF045_RS01595 | | GspE/PulE family protein | insig. | 31 | 66 |
| | Shewanella amazonensis SB2B | Sama_2475 | | twitching motility protein PilU (RefSeq) | insig. | 40 | 30 |
| | Shewanella amazonensis SB2B | Sama_0457 | | MSHA biogenesis protein MshE (RefSeq) | cofit | 34 | 35 |
| | Shewanella sp. ANA-3 | Shewana3_1196 | | twitching motility protein (RefSeq) | insig. | 40 | 30 |
| | Shewanella oneidensis MR-1 | SO3350 | pilU | twitching motility protein PilU (NCBI ptt file) | strong | 40 | 30 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS22670 | | type II/IV secretion system protein | insig. | 39 | 29 |
| | Ralstonia solanacearum GMI1000 | RS_RS17860 | | type II/IV secretion system protein | no data | 39 | 29 |
| | Ralstonia solanacearum UW163 | UW163_RS23265 | | type II/IV secretion system protein | insig. | 39 | 29 |
| | Shewanella loihica PV-4 | Shew_1139 | | twitching motility protein (RefSeq) | cofit | 40 | 30 |
| | Lysobacter sp. OAE881 | ABIE51_RS13600 | | GspE/PulE family protein | insig. | 41 | 28 |
| | Pseudomonas sp. S08-1 | OH686_14715 | | MSHA biogenesis protein MshE | insig. | 34 | 28 |
| | Marinobacter adhaerens HP15 | HP15_416 | | type IV-A pilus assembly ATPase PilB | cofit | 26 | 68 |
| | Synechococcus elongatus PCC 7942 | Synpcc7942_0847 | pilT | twitching motility protein | sig. | 31 | 66 |
| | Shewanella sp. ANA-3 | Shewana3_3660 | | type II secretion system protein E (RefSeq) | insig. | 33 | 35 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS27305 | | ATPase, T2SS/T4P/T4SS family | insig. | 38 | 28 |
| | Methylophilus sp. DMC18 | GFF1853 | | Type II secretion system protein E | insig. | 35 | 33 |
| | Shewanella oneidensis MR-1 | SO4109 | mshE | MSHA biogenesis protein MshE (NCBI ptt file) | strong | 34 | 33 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF66 | | Predicted secretion system X protein GspE-like | no data | 35 | 38 |
| | Shewanella loihica PV-4 | Shew_0392 | | type II secretion system protein E (RefSeq) | strong | 33 | 33 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS13950 | | GspE/PulE family protein | insig. | 38 | 28 |
| | Methylophilus sp. DMC18 | GFF6 | | Type II secretion system protein E | insig. | 35 | 33 |
| | Marinobacter adhaerens HP15 | HP15_2409 | | ATPase, type IV, pilus assembly, PilB | cofit | 32 | 45 |
| | Pseudomonas sp. BP01 | JOY50_RS06170 | | GspE/PulE family protein | insig. | 31 | 39 |
| | Pseudomonas putida KT2440 | PP_3483 | | putative Type II secretion system protein | insig. | 31 | 39 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_05365 | | hypothetical protein | cofit | 29 | 48 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_3847 | | Predicted secretion system X protein GspE-like | insig. | 35 | 33 |
| | Dechlorosoma suillum PS | Dsui_2710 | | pilus retraction protein PilT | insig. | 33 | 38 |
| | Escherichia coli BL21 | ECD_03177 | | general secretory pathway component, cryptic | insig. | 38 | 28 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00377 | | Putative type II secretion system protein E | insig. | 38 | 28 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS19065 | | type II secretion system ATPase GspE | insig. | 38 | 28 |
| | Escherichia coli BW25113 | b3326 | gspE | general secretory pathway component, cryptic (NCBI) | insig. | 38 | 28 |
| | Pantoea sp. MT58 | IAI47_15855 | | type II secretion system protein GspE | insig. | 35 | 30 |
| | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS13145 | | type II secretion system protein GspE | insig. | 35 | 30 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_2078 | | type II secretion system protein E | insig. | 31 | 53 |
| | Escherichia fergusonii Becca | EFB2_00497 | | Putative type II secretion system protein E | cofit | 38 | 28 |
| | Pectobacterium carotovorum WPP14 | HER17_RS03240 | | type II secretion system protein GspE | insig. | 36 | 34 |
| | Paraburkholderia graminis OAS925 | ABIE53_003594 | | type IV pilus assembly protein PilB | insig. | 34 | 33 |
| | Pseudomonas segetis P6 | ACVTMO_RS11710 | | PilT/PilU family type 4a pilus ATPase | insig. | 33 | 36 |
| | Pseudomonas sp. SVBP6 | COO64_RS00940 | | GspE/PulE family protein | insig. | 32 | 43 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS17980 | | general secretion pathway protein GspE | insig. | 37 | 32 |
| | Serratia liquefaciens MT49 | IAI46_03520 | | type II secretion system protein GspE | insig. | 36 | 30 |
| | Methylophilus sp. DMC18 | GFF1666 | | Type II secretion system protein E | cofit | 35 | 28 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS17145 | | type II secretion system protein E | insig. | 34 | 34 |
| | Acinetobacter radioresistens SK82 | MPMX26_02406 | | Twitching mobility protein | insig. | 35 | 29 |
| | Alteromonas macleodii MIT1002 | MIT1002_03060 | | Twitching mobility protein | insig. | 29 | 41 |
| | Pseudomonas simiae WCS417 | PS417_26720 | | twitching motility protein PilT | insig. | 29 | 41 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_3700 | | Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | insig. | 32 | 39 |
| | Alteromonas macleodii MIT1002 | MIT1002_00210 | | Type II traffic warden ATPase | cofit | 27 | 61 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_05845 | gspE | type II secretion system protein GspE | cofit | 36 | 30 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_02135 | | MSHA biogenesis protein MshE | cofit | 35 | 28 |
| | Enterobacter sp. TBS_079 | MPMX20_01516 | | Putative type II secretion system protein E | insig. | 35 | 31 |
| | Pseudomonas sp. S08-1 | OH686_00770 | | Twitching motility protein PilT | insig. | 33 | 36 |
| | Rahnella sp. WP5 | EX31_RS02680 | | type IV pilus twitching motility protein PilT | insig. | 31 | 37 |
| | Dickeya dadantii 3937 | DDA3937_RS17965 | | type II secretion system protein GspE | insig. | 30 | 36 |
| | Erwinia tracheiphila SCR3 | LU632_RS03030 | gspE | type II secretion system protein GspE | insig. | 36 | 30 |
| | Dechlorosoma suillum PS | Dsui_2996 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | insig. | 37 | 28 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06257 | | type IV pilus assembly protein PilB | insig. | 36 | 28 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_2906 | | Pilus retraction protein PilT | sig. | 34 | 39 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_2906 | | Pilus retraction protein PilT | insig. | 34 | 39 |
| | Pseudomonas putida KT2440 | PP_5190 | | Type II secretion system protein | insig. | 39 | 28 |
| | Variovorax sp. OAS795 | ABID97_RS23430 | | type IV-A pilus assembly ATPase PilB | strong | 35 | 28 |
| | Variovorax sp. SCN45 | GFF2510 | | Type IV fimbrial assembly, ATPase PilB | strong | 35 | 28 |
| | Pseudomonas sp. S08-1 | OH686_10965 | | Type IV pilus assembly ATPase component PilU | insig. | 33 | 40 |
| | Mucilaginibacter yixingensis YX-36 DSM 26809 | ABZR88_RS02570 | | GspE/PulE family protein | insig. | 37 | 34 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS11060 | | type IV-A pilus assembly ATPase PilB | cofit | 35 | 28 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU1272 | gspE | general secretion pathway protein E, putative (TIGR) | strong | 34 | 32 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS17345 | | twitching motility protein PilT | insig. | 33 | 29 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS28880 | | type II/IV secretion system protein | insig. | 31 | 64 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS28880 | | type II/IV secretion system protein | insig. | 31 | 64 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS06180 | | type IV-A pilus assembly ATPase PilB | sig. | 35 | 28 |
| | Ralstonia solanacearum GMI1000 | RS_RS14130 | | type IV-A pilus assembly ATPase PilB | insig. | 35 | 28 |
| | Ralstonia solanacearum UW163 | UW163_RS07445 | | type IV-A pilus assembly ATPase PilB | insig. | 35 | 28 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_4766 | | Type IV fimbrial assembly, ATPase PilB | insig. | 34 | 30 |
| | Pseudomonas orientalis W4I3 | QF045_RS19490 | | ATPase, T2SS/T4P/T4SS family | cofit | 33 | 28 |
| | Dickeya dianthicola ME23 | DZA65_RS19045 | | type II secretion system protein GspE | insig. | 30 | 37 |
| | Pseudomonas sp. BP01 | JOY50_RS22395 | | GspE/PulE family protein | insig. | 39 | 28 |
| | Methylophilus sp. DMC18 | GFF275 | | hypothetical protein | insig. | 36 | 28 |
| | Shewanella amazonensis SB2B | Sama_0369 | | type IV pilus biogenesis protein PilB (RefSeq) | insig. | 34 | 30 |
| | Shewanella loihica PV-4 | Shew_3438 | | type IV-A pilus assembly ATPase PilB (RefSeq) | strong | 32 | 33 |
| | Dickeya dianthicola 67-19 | HGI48_RS17900 | | type II secretion system protein GspE | insig. | 30 | 37 |
| | Enterobacter sp. TBS_079 | MPMX20_03808 | | putative protein YggR | insig. | 30 | 36 |
| | Pseudomonas orientalis W4I3 | QF045_RS01120 | | type IV pilus twitching motility protein PilT | insig. | 28 | 41 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS01845 | | type IV-A pilus assembly ATPase PilB | strong | 35 | 28 |
| | Acinetobacter baumannii LAC-4 | RR41_RS14805 | | PilT/PilU family type 4a pilus ATPase | insig. | 35 | 29 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS21960 | pilU;inference=COORDINATES | type IV pilus ATPase PilU | strong | 33 | 40 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_23440 | | twitching motility protein PilU | sig. | 33 | 40 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS37340 | pilU;inference=COORDINATES | type IV pilus ATPase PilU | strong | 33 | 40 |
| | Pseudomonas stutzeri RCH2 | Psest_0286 | | pilus retraction protein PilT | insig. | 33 | 40 |
| | Pseudomonas segetis P6 | ACVTMO_RS03030 | | PilT/PilU family type 4a pilus ATPase | sig. | 32 | 40 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_08710 | | type II secretion system protein E | insig. | 34 | 28 |
| | Dechlorosoma suillum PS | Dsui_1320 | | type IV-A pilus assembly ATPase PilB | insig. | 34 | 28 |
| | Lysobacter sp. OAE881 | ABIE51_RS04520 | | type II secretion system ATPase GspE | strong | 32 | 30 |
| | Dechlorosoma suillum PS | Dsui_1279 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | strong | 31 | 33 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_05583 | | hypothetical protein | cofit | 30 | 65 |
| | Marinobacter adhaerens HP15 | HP15_1264 | | type IV-A pilus assembly ATPase PilB | insig. | 30 | 43 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS28365 | | type IV pilus twitching motility protein PilT | insig. | 27 | 41 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS28365 | | type IV pilus twitching motility protein PilT | insig. | 27 | 41 |
| | Dechlorosoma suillum PS | Dsui_0605 | | pilus retraction protein PilT | insig. | 34 | 29 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_2297 | | Twitching motility protein PilT | insig. | 27 | 51 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS01890 | | GspE/PulE family protein | insig. | 36 | 28 |
| | Pseudomonas sp. S08-1 | OH686_08925 | | Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 40 | 30 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS03825 | | GspE/PulE family protein | insig. | 33 | 28 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_13445 | | type II secretion system protein | insig. | 33 | 28 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS34860 | | GspE/PulE family protein | insig. | 33 | 28 |
| | Variovorax sp. SCN45 | GFF3742 | | Type IV pilus assembly ATPase component PilU | no data | 35 | 29 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS10040 | | type IV-A pilus assembly ATPase PilB | cofit | 33 | 30 |
| | Erwinia amylovora T8 | OLJFJH_08535 | | type IV pili twitching motility protein PilT | insig. | 38 | 28 |
| | Methylophilus sp. DMC18 | GFF866 | | Twitching mobility protein | insig. | 34 | 29 |
| | Acinetobacter baumannii LAC-4 | RR41_RS06140 | | Flp pilus assembly complex ATPase component TadA | insig. | 33 | 39 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_05481 | | Twitching mobility protein | cofit | 28 | 41 |
| | Rhodospirillum rubrum S1H | Rru_A0113 | | type II secretion system protein E (NCBI) | insig. | 37 | 28 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1279 | | Type IV fimbrial assembly, ATPase PilB | cofit | 35 | 28 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_2988 | | pilus retraction protein PilT | insig. | 33 | 28 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.4432.1 | | General secretion pathway protein E | strong | 33 | 28 |