| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Variovorax sp. SCN45 | GFF4849 | | Conjugative transfer protein TrbB | insig. | 100 | 100 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4435 | | Conjugative transfer protein TrbB | insig. | 90 | 100 |
| | Variovorax sp. SCN45 | GFF6579 | | Conjugative transfer protein TrbB | insig. | 93 | 100 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4176 | | Conjugative transfer protein TrbB | insig. | 93 | 95 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_12825 | | P-type conjugative transfer ATPase TrbB | no data | 88 | 100 |
| | Variovorax sp. SCN45 | GFF4414 | | Conjugative transfer protein TrbB | insig. | 91 | 94 |
| | Ralstonia solanacearum GMI1000 | RS_RS12955 | | P-type conjugative transfer ATPase TrbB | insig. | 84 | 100 |
| | Shewanella sp. ANA-3 | Shewana3_1282 | | type II secretion system protein E (RefSeq) | cofit | 84 | 100 |
| | Dickeya dianthicola 67-19 | HGI48_RS03925 | | P-type conjugative transfer ATPase TrbB | insig. | 85 | 99 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS00155 | | P-type conjugative transfer ATPase TrbB | insig. | 82 | 99 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF3453 | | Conjugative transfer protein TrbB | insig. | 80 | 95 |
| o | Dickeya dadantii 3937 | DDA3937_RS03995 | | P-type conjugative transfer ATPase TrbB | insig. | 82 | 89 |
| | Dickeya dianthicola 67-19 | HGI48_RS08420 | | P-type conjugative transfer ATPase TrbB | insig. | 77 | 96 |
| | Rhodospirillum rubrum S1H | Rru_A0808 | | type II secretion system protein E (NCBI) | insig. | 63 | 86 |
| o | Rhodopseudomonas palustris CGA009 | TX73_021370 | | P-type conjugative transfer ATPase TrbB | insig. | 62 | 88 |
| o | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2572 | | P-type conjugative transfer ATPase TrbB | insig. | 63 | 88 |
| | Rhodopseudomonas palustris CGA009 | TX73_011525 | | P-type conjugative transfer ATPase TrbB | insig. | 59 | 92 |
| | Xanthobacter sp. DMC5 | GFF2635 | | hypothetical protein | insig. | 59 | 91 |
| | Xanthobacter sp. DMC5 | GFF3638 | | hypothetical protein | insig. | 59 | 91 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0389 | | P-type conjugative transfer ATPase TrbB | insig. | 58 | 89 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2912 | | P-type conjugative transfer ATPase TrbB | insig. | 58 | 89 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_3072 | | P-type conjugative transfer ATPase TrbB | insig. | 58 | 88 |
| | Rhodopseudomonas palustris CGA009 | TX73_021310 | | P-type conjugative transfer ATPase TrbB | insig. | 64 | 77 |
| | Sphingobium sp. HT1-2 | GFF188 | | Conjugative transfer protein TrbB | cofit | 57 | 83 |
| o | Agrobacterium fabrum C58 | Atu6041 | | conjugal transfer protein | insig. | 49 | 88 |
| o | Marinobacter adhaerens HP15 | HP15_p42g52 | | type II secretion system protein E | cofit | 45 | 90 |
| | Paraburkholderia graminis OAS925 | ABIE53_004998 | | pilus assembly protein CpaF | sig. | 38 | 83 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06368 | | pilus assembly protein CpaF | no data | 37 | 83 |
| | Shewanella amazonensis SB2B | Sama_0708 | | type II/IV secretion system protein E (RefSeq) | insig. | 32 | 92 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS22920 | | pilus assembly protein CpaF | insig. | 35 | 83 |
| | Pseudomonas sp. RS175 | PFR28_03044 | | hypothetical protein | insig. | 37 | 81 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_01570 | | pilus assembly protein CpaF | insig. | 36 | 81 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_496 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 36 | 81 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_19980 | | pilus assembly protein CpaF | insig. | 36 | 81 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5915 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 36 | 81 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS08500 | | CpaF family protein | insig. | 36 | 83 |
| | Variovorax sp. SCN45 | GFF2208 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 38 | 86 |
| | Ralstonia solanacearum GMI1000 | RS_RS03280 | | CpaF family protein | insig. | 35 | 83 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS10645 | | CpaF family protein | insig. | 34 | 83 |
| | Variovorax sp. OAS795 | ABID97_RS24290 | | CpaF family protein | no data | 38 | 86 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2070 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 36 | 81 |
| | Shewanella loihica PV-4 | Shew_0644 | | FHA domain-containing protein (RefSeq) | insig. | 31 | 92 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS12510 | | CpaF family protein | insig. | 36 | 83 |
| | Ralstonia solanacearum UW163 | UW163_RS01055 | | CpaF family protein | insig. | 36 | 83 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS04100 | | pilus assembly protein CpaF | insig. | 35 | 83 |
| | Pectobacterium carotovorum WPP14 | HER17_RS17705 | | CpaF family protein | insig. | 35 | 86 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4121 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | no data | 39 | 83 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS08110 | | CpaF family protein | insig. | 35 | 83 |
| | Pseudomonas sp. SVBP6 | COO64_RS23430 | | CpaF family protein | insig. | 34 | 81 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS19545 | | CpaF family protein | strong | 39 | 83 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS35985 | | pilus assembly protein CpaF | sig. | 39 | 83 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS18950 | | CpaF family protein | insig. | 36 | 87 |
| | Sphingobium sp. HT1-2 | GFF4554 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | cofit | 34 | 81 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS18510 | | CpaF family protein | insig. | 38 | 83 |
| | Ralstonia solanacearum UW163 | UW163_RS20620 | | CpaF family protein | insig. | 38 | 83 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2738 | | type II secretion system protein E (RefSeq) | insig. | 33 | 86 |
| | Ralstonia solanacearum GMI1000 | RS_RS22355 | | CpaF family protein | insig. | 39 | 83 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS05045 | | CpaF family protein | insig. | 39 | 83 |
| | Variovorax sp. OAS795 | ABID97_RS09105 | | CpaF family protein | insig. | 34 | 87 |
| | Rhizobium sp. OAE497 | ABIE40_RS00885 | | CpaF family protein | insig. | 35 | 80 |
| | Rhodopseudomonas palustris CGA009 | TX73_019075 | | CpaF family protein | insig. | 35 | 83 |
| | Agrobacterium fabrum C58 | Atu0218 | | component of type IV pilus | insig. | 37 | 70 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2122 | cpaF | type II/IV secretion system protein (TIGR) | insig. | 32 | 81 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS43755 | | ATPase, T2SS/T4P/T4SS family | insig. | 32 | 87 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS17490 | | ATPase, T2SS/T4P/T4SS family | insig. | 33 | 92 |
| | Sinorhizobium meliloti 1021 | SMa1568 | | CpaF2 pilus assembly protein | insig. | 35 | 80 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS04365 | | ATPase, T2SS/T4P/T4SS family | insig. | 33 | 91 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS12005 | | CpaF family protein | insig. | 35 | 83 |
| | Paraburkholderia graminis OAS925 | ABIE53_002438 | | pilus assembly protein CpaF | cofit | 36 | 83 |
| | Sinorhizobium meliloti 1021 | SMc02820 | | pilus assembly protein | insig. | 38 | 70 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS20760 | | CpaF family protein | insig. | 35 | 83 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS08185 | | pilus assembly protein CpaF | insig. | 35 | 83 |
| | Pseudomonas sp. S08-1 | OH686_19900 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 34 | 83 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0975 | | Flp pilus assembly protein, ATPase CpaF | insig. | 32 | 81 |
| | Bosea sp. OAE506 | ABIE41_RS03685 | | CpaF family protein | insig. | 37 | 70 |
| | Caulobacter crescentus NA1000 | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 35 | 80 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 35 | 80 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_13820 | | ATPase | insig. | 35 | 83 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS34040 | | hypothetical protein | insig. | 32 | 86 |
| | Pseudomonas segetis P6 | ACVTMO_RS20025 | | CpaF family protein | insig. | 33 | 85 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_1278 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 34 | 85 |
| | Pseudomonas stutzeri RCH2 | Psest_2619 | | Flp pilus assembly protein, ATPase CpaF | insig. | 38 | 70 |
| | Pseudomonas sp. S08-1 | OH686_02520 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 35 | 81 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS03175 | | CpaF family protein | insig. | 35 | 79 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS03175 | | CpaF family protein | strong | 35 | 79 |
| | Pseudomonas sp. DMC3 | GFF3341 | | hypothetical protein | cofit | 34 | 83 |
| | Pseudomonas orientalis W4I3 | QF045_RS06130 | | CpaF family protein | cofit | 34 | 83 |
| | Pseudomonas simiae WCS417 | PS417_03095 | | ATPase | sig. | 34 | 79 |
| | Pseudomonas sp. SVBP6 | COO64_RS18465 | | CpaF family protein | insig. | 34 | 85 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS24995 | tadA;inference=COORDINATES | type 4b pilus Flp biogenesis ATPase TadA | insig. | 36 | 81 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_02600 | | type 4b pilus Flp biogenesis ATPase TadA | sig. | 36 | 81 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS57450 | tadA;inference=COORDINATES | type 4b pilus Flp biogenesis ATPase TadA | sig. | 36 | 81 |
| | Caulobacter crescentus NA1000 | CCNA_02505 | | type IV secretory pathway, VirB11 ATPase | sig. | 32 | 79 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_02505 | | type IV secretory pathway, VirB11 ATPase | cofit | 32 | 79 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c06110 | | Flp pilus assembly protein, ATPase CpaF | insig. | 34 | 84 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS01870 | | P-type DNA transfer ATPase VirB11 | insig. | 35 | 74 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_4393 | | type II secretion system protein E | sig. | 33 | 85 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_4393 | | type II secretion system protein E | sig. | 33 | 85 |
| | Marinobacter adhaerens HP15 | HP15_p187g9 | | type II secretion system protein E | strong | 38 | 64 |
| | Dechlorosoma suillum PS | Dsui_2579 | | Flp pilus assembly protein, ATPase CpaF | cofit | 31 | 86 |
| | Dinoroseobacter shibae DFL-12 | Dshi_1126 | | type II secretion system protein E (RefSeq) | insig. | 33 | 83 |
| | Escherichia coli ECOR38 | HEPCGN_00330 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 24 | 87 |
| | Agrobacterium fabrum C58 | Atu5172 | | type IV secretion protein AvhB11 | insig. | 31 | 79 |
| | Sinorhizobium meliloti 1021 | SMa1302 | | VirB11-like ATPase | insig. | 30 | 79 |
| | Acinetobacter radioresistens SK82 | MPMX26_03199 | | Type IV secretion system protein VirB11 | insig. | 26 | 75 |
| | Mycobacterium tuberculosis H37Rv | Rv3659c | | Conserved hypothetical protein | insig. | 32 | 77 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3983 | | type II secretion system protein E (RefSeq) | insig. | 30 | 75 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3648 | | type II secretion system protein E (RefSeq) | insig. | 30 | 75 |
| | Escherichia coli ECOR27 | NOLOHH_00295 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 27 | 70 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c22920 | | putative type IV secretion system protein VirB11 | insig. | 28 | 67 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.2046.1 | | hypothetical protein | insig. | 24 | 80 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS06740 | | P-type DNA transfer ATPase VirB11 | insig. | 29 | 79 |
| | Bifidobacterium breve UCC2003 | BBR_RS10950 | | pilus biosynthesis protein | insig. | 38 | 59 |
| | Methanococcus maripaludis JJ | MMJJ_RS05235 | | Flp pilus assembly complex ATPase component TadA | insig. | 30 | 82 |
| | Methanococcus maripaludis S2 | MMP_RS00250 | | Flp pilus assembly complex ATPase component TadA | strong | 30 | 82 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS32155 | | type IV secretion system protein VirB11 | sig. | 30 | 79 |
| | Dickeya dianthicola 67-19 | HGI48_RS07090 | | P-type DNA transfer ATPase VirB11 | insig. | 30 | 62 |
| | Agrobacterium fabrum C58 | Atu6177 | | component of type IV secretion system | insig. | 28 | 80 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11415 | | P-type DNA transfer ATPase VirB11 | insig. | 25 | 80 |
| | Dickeya dadantii 3937 | DDA3937_RS13875 | | P-type DNA transfer ATPase VirB11 | insig. | 29 | 62 |
| | Ralstonia solanacearum UW163 | UW163_RS23875 | | P-type DNA transfer ATPase VirB11 | insig. | 29 | 70 |
| | Methanococcus maripaludis JJ | MMJJ_RS05745 | | type II/IV secretion system ATPase subunit | cofit | 28 | 73 |
| | Methanococcus maripaludis S2 | MMP_RS08625 | | type II/IV secretion system ATPase subunit | cofit | 27 | 73 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS40910 | | P-type DNA transfer ATPase VirB11 | no data | 35 | 50 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS00810 | | type IV secretion system protein VirB11 | insig. | 35 | 42 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS03320 | | P-type DNA transfer ATPase VirB11 | insig. | 36 | 37 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS36345 | | hypothetical protein | insig. | 28 | 69 |
| | Dickeya dianthicola ME23 | DZA65_RS14430 | | P-type DNA transfer ATPase VirB11 | insig. | 28 | 62 |
| | Escherichia coli ECOR27 | NOLOHH_00210 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 27 | 88 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS22670 | | type II/IV secretion system protein | insig. | 32 | 34 |
| | Ralstonia solanacearum GMI1000 | RS_RS17860 | | type II/IV secretion system protein | no data | 32 | 34 |
| | Ralstonia solanacearum UW163 | UW163_RS23265 | | type II/IV secretion system protein | insig. | 32 | 34 |
| | Methylophilus sp. DMC18 | GFF6 | | Type II secretion system protein E | insig. | 32 | 33 |
| | Pseudomonas putida KT2440 | PP_3483 | | putative Type II secretion system protein | insig. | 30 | 39 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_3847 | | Predicted secretion system X protein GspE-like | insig. | 31 | 39 |
| | Azospirillum brasilense Sp245 | AZOBR_RS33110 | | hypothetical protein | insig. | 28 | 70 |
| | Pseudomonas sp. BP01 | JOY50_RS06170 | | GspE/PulE family protein | insig. | 30 | 39 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF66 | | Predicted secretion system X protein GspE-like | no data | 29 | 53 |
| | Ralstonia solanacearum GMI1000 | RS_RS19385 | | type II/IV secretion system protein | insig. | 32 | 38 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS13920 | | type II/IV secretion system protein | insig. | 33 | 32 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_1886 | | General secretion pathway protein E | no data | 32 | 32 |
| | Methylophilus sp. DMC18 | GFF1853 | | Type II secretion system protein E | insig. | 28 | 39 |
| | Pseudomonas sp. S08-1 | OH686_08925 | | Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 35 | 36 |
| | Shewanella sp. ANA-3 | Shewana3_3660 | | type II secretion system protein E (RefSeq) | insig. | 28 | 53 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS13950 | | GspE/PulE family protein | insig. | 31 | 33 |
| | Shewanella amazonensis SB2B | Sama_0457 | | MSHA biogenesis protein MshE (RefSeq) | cofit | 27 | 45 |
| | Pseudomonas sp. S08-1 | OH686_16505 | | type IV-A pilus assembly ATPase PilB | insig. | 31 | 43 |
| | Variovorax sp. SCN45 | GFF103 | | General secretion pathway protein E | insig. | 30 | 37 |
| | Shewanella oneidensis MR-1 | SO4109 | mshE | MSHA biogenesis protein MshE (NCBI ptt file) | strong | 28 | 39 |
| | Pseudomonas simiae WCS417 | PS417_18085 | | general secretion pathway protein GspE | insig. | 28 | 54 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_16015 | | general secretion pathway protein GspE | insig. | 32 | 43 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5142 | | Type IV fimbrial assembly, ATPase PilB | insig. | 32 | 43 |
| | Shewanella sp. ANA-3 | Shewana3_1196 | | twitching motility protein (RefSeq) | insig. | 31 | 43 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS04100 | | type II/IV secretion system protein | insig. | 30 | 33 |
| | Ralstonia solanacearum UW163 | UW163_RS09570 | | type II/IV secretion system protein | insig. | 30 | 33 |
| | Shewanella loihica PV-4 | Shew_0392 | | type II secretion system protein E (RefSeq) | strong | 28 | 39 |
| | Ralstonia solanacearum GMI1000 | RS_RS11580 | | type II/IV secretion system protein | insig. | 30 | 33 |
| | Rhodospirillum rubrum S1H | Rru_A0118 | | type II secretion system protein E (NCBI) | insig. | 38 | 34 |
| | Pseudomonas sp. RS175 | PFR28_03731 | | Type II secretion system protein E | insig. | 32 | 43 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_02135 | | MSHA biogenesis protein MshE | cofit | 31 | 32 |
| | Dechlorosoma suillum PS | Dsui_1393 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 31 | 46 |
| | Escherichia coli BL21 | ECD_03177 | | general secretory pathway component, cryptic | insig. | 34 | 34 |
| | Escherichia fergusonii Becca | EFB2_00497 | | Putative type II secretion system protein E | cofit | 34 | 34 |
| | Mucilaginibacter yixingensis YX-36 DSM 26809 | ABZR88_RS02570 | | GspE/PulE family protein | insig. | 31 | 39 |
| | Shewanella oneidensis MR-1 | SO3350 | pilU | twitching motility protein PilU (NCBI ptt file) | strong | 30 | 43 |
| | Caulobacter crescentus NA1000 | CCNA_00173 | | type II secretion pathway protein E | cofit | 35 | 36 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_00173 | | type II secretion pathway protein E | cofit | 35 | 36 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00377 | | Putative type II secretion system protein E | insig. | 34 | 34 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS19065 | | type II secretion system ATPase GspE | insig. | 34 | 34 |
| | Escherichia coli BW25113 | b3326 | gspE | general secretory pathway component, cryptic (NCBI) | insig. | 34 | 34 |
| | Pectobacterium carotovorum WPP14 | HER17_RS06690 | | type II secretion system ATPase GspE | insig. | 34 | 34 |
| | Azospirillum sp. SherDot2 | MPMX19_00280 | | hypothetical protein | insig. | 33 | 38 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS09600 | | type II secretion system ATPase GspE | cofit | 32 | 32 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_14330 | | general secretion pathway protein GspE | insig. | 32 | 43 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS30925 | | GspE/PulE family protein | insig. | 29 | 39 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_05365 | | hypothetical protein | cofit | 28 | 39 |
| | Acinetobacter baumannii LAC-4 | RR41_RS15895 | | type II secretion system ATPase GspE | sig. | 32 | 34 |
| | Acinetobacter radioresistens SK82 | MPMX26_00578 | | Type II secretion system protein E | insig. | 31 | 39 |
| | Lysobacter sp. OAE881 | ABIE51_RS13600 | | GspE/PulE family protein | insig. | 30 | 32 |
| | Pseudomonas sp. DMC3 | GFF4018 | | hypothetical protein | cofit | 31 | 44 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS09960 | | type II secretion system ATPase GspE | insig. | 32 | 32 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS02730 | | type II secretion system ATPase GspE | cofit | 32 | 32 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS23835 | pilB;go_function=ATP | type IV-A pilus assembly ATPase PilB | strong | 31 | 43 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_01445 | | type 4 fimbrial biogenesis protein PilB | strong | 31 | 43 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS56295 | pilB;go_function=ATP | type IV-A pilus assembly ATPase PilB | strong | 31 | 43 |
| | Pseudomonas sp. S08-1 | OH686_14715 | | MSHA biogenesis protein MshE | insig. | 29 | 39 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_2842 | | Type IV fimbrial assembly, ATPase PilB | insig. | 32 | 37 |
| | Dickeya dianthicola 67-19 | HGI48_RS15455 | | Flp pilus assembly complex ATPase component TadA | insig. | 28 | 39 |
| | Dickeya dianthicola ME23 | DZA65_RS16275 | | Flp pilus assembly complex ATPase component TadA | insig. | 28 | 39 |
| | Pseudomonas sp. SVBP6 | COO64_RS04780 | | GspE/PulE family protein | insig. | 34 | 40 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_3700 | | Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | insig. | 32 | 45 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU1272 | gspE | general secretion pathway protein E, putative (TIGR) | strong | 35 | 34 |
| | Shewanella loihica PV-4 | Shew_3618 | | general secretory pathway protein E (RefSeq) | strong | 34 | 34 |
| | Alteromonas macleodii MIT1002 | MIT1002_03059 | | Twitching mobility protein | insig. | 31 | 33 |
| | Pseudomonas stutzeri RCH2 | Psest_3288 | | type IV-A pilus assembly ATPase PilB | strong | 30 | 43 |
| | Escherichia coli ECOR38 | HEPCGN_08520 | gspE | type II secretion system protein GspE | insig. | 29 | 33 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS00570 | | type II secretion system protein GspE | insig. | 29 | 33 |
| | Shewanella sp. ANA-3 | Shewana3_0152 | | type II secretion system protein E (GspE) (RefSeq) | strong | 31 | 34 |
| | Shewanella loihica PV-4 | Shew_1139 | | twitching motility protein (RefSeq) | cofit | 30 | 43 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_0798 | | type II secretion system protein E:General secretory system II, protein E, N-terminal | insig. | 30 | 43 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_0798 | | type II secretion system protein E:General secretory system II, protein E, N-terminal | insig. | 30 | 43 |
| | Fusobacterium nucleatum SB010 | HUW76_00165 | | type II/IV secretion system protein | strong | 26 | 40 |
| | Escherichia fergusonii Becca | EFB2_03942 | | Type II secretion system protein E | insig. | 33 | 33 |
| | Escherichia coli BL21 | ECD_02837 | | putative type II secretion protein GspE | strong | 31 | 34 |
| | Escherichia fergusonii Becca | EFB2_00846 | | Type II secretion system protein E | insig. | 31 | 34 |
| | Escherichia coli ECOR27 | NOLOHH_10980 | gspE | general secretion pathway protein E | sig. | 31 | 34 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00740 | | Type II secretion system protein E | cofit | 31 | 34 |
| | Shewanella oneidensis MR-1 | SO0167 | gspE | general secretion pathway protein E (NCBI ptt file) | strong | 31 | 34 |
| | Escherichia coli BL21 | ECD_00106 | | T2SE secretion family protein; P-loop ATPase superfamily protein | insig. | 29 | 33 |
| | Escherichia coli ECRC100 | OKFHMN_10540 | gspE | type II secretion system protein GspE | cofit | 29 | 33 |
| | Escherichia coli ECRC101 | MCAODC_01055 | gspE | type II secretion system protein GspE | insig. | 29 | 33 |
| | Escherichia coli ECRC102 | NIAGMN_08390 | gspE | type II secretion system protein GspE | cofit | 29 | 33 |
| | Escherichia coli ECRC98 | JDDGAC_14135 | gspE | type II secretion system protein GspE | insig. | 29 | 33 |
| | Escherichia coli ECRC99 | KEDOAH_17615 | gspE | type II secretion system protein GspE | no data | 29 | 33 |
| | Escherichia coli BW25113 | b0107 | hofB | conserved protein with nucleoside triphosphate hydrolase domain (NCBI) | insig. | 29 | 33 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS27305 | | ATPase, T2SS/T4P/T4SS family | insig. | 28 | 50 |
| | Dickeya dadantii 3937 | DDA3937_RS12695 | | Flp pilus assembly complex ATPase component TadA | insig. | 28 | 39 |
| | Dechlorosoma suillum PS | Dsui_1279 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | strong | 28 | 39 |
| | Escherichia coli ECOR38 | HEPCGN_18055 | gspE | general secretion pathway protein E | strong | 31 | 34 |
| | Erwinia tracheiphila SCR3 | LU632_RS25235 | gspE | type II secretion system ATPase GspE | insig. | 28 | 48 |
| | Dechlorosoma suillum PS | Dsui_1455 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 32 | 32 |
| | Alteromonas macleodii MIT1002 | MIT1002_03977 | | Type II traffic warden ATPase | strong | 32 | 41 |
| | Shewanella amazonensis SB2B | Sama_2475 | | twitching motility protein PilU (RefSeq) | insig. | 30 | 43 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_09695 | gspE | type II secretion system ATPase GspE | cofit | 28 | 48 |
| | Pseudomonas segetis P6 | ACVTMO_RS19350 | pilB | type IV-A pilus assembly ATPase PilB | cofit | 30 | 43 |
| | Escherichia coli ECOR27 | NOLOHH_02885 | gspE | type II secretion system protein GspE | insig. | 29 | 33 |
| | Escherichia coli ECRC62 | BNILDI_08725 | gspE | type II secretion system protein GspE | insig. | 29 | 33 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_03554 | | Type II secretion system protein E | insig. | 29 | 33 |
| | Enterobacter sp. TBS_079 | MPMX20_01516 | | Putative type II secretion system protein E | insig. | 33 | 34 |
| | Rahnella sp. WP5 | EX31_RS16170 | | type II secretion system ATPase GspE | insig. | 33 | 34 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_3151 | | type II secretion system protein E (GspE) | insig. | 26 | 54 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3151 | | type II secretion system protein E (GspE) | insig. | 26 | 54 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1028 | | type II secretion system protein E (RefSeq) | cofit | 36 | 32 |
| | Marinobacter adhaerens HP15 | HP15_2290 | | type IV-A pilus assembly ATPase PilB | cofit | 33 | 33 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS13115 | | type II secretion system ATPase GspE | strong | 31 | 32 |
| | Rahnella sp. WP5 | EX31_RS03525 | | type II secretion system protein GspE | insig. | 29 | 34 |
| | Shewanella amazonensis SB2B | Sama_0170 | | general secretion pathway protein E (RefSeq) | strong | 32 | 34 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS11735 | | twitching motility protein PilT | insig. | 27 | 51 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_03878 | | Type II secretion system protein E | cofit | 26 | 54 |
| | Enterobacter sp. TBS_079 | MPMX20_00798 | | Type II secretion system protein E | insig. | 33 | 39 |
| | Dickeya dadantii 3937 | DDA3937_RS14795 | | type II secretion system ATPase GspE | cofit | 32 | 34 |
| | Dickeya dianthicola 67-19 | HGI48_RS14730 | | type II secretion system ATPase GspE | insig. | 32 | 34 |
| | Acinetobacter baumannii LAC-4 | RR41_RS17340 | | type IV-A pilus assembly ATPase PilB | insig. | 30 | 43 |
| | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS13145 | | type II secretion system protein GspE | insig. | 29 | 39 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_2078 | | type II secretion system protein E | insig. | 33 | 34 |
| | Klebsiella pneumoniae MRSN742743 | KFA93_RS11785 | gspE;go_component=type | type II secretion system ATPase GspE | insig. | 33 | 34 |
| | Dickeya dianthicola ME23 | DZA65_RS15675 | | type II secretion system ATPase GspE | cofit | 32 | 34 |
| | Paraburkholderia graminis OAS925 | ABIE53_003594 | | type IV pilus assembly protein PilB | insig. | 32 | 32 |
| | Acinetobacter radioresistens SK82 | MPMX26_00246 | | hypothetical protein | cofit | 30 | 43 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_02355 | | type II secretion system protein E (GspE) | cofit | 28 | 46 |