| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Agrobacterium fabrum C58 | Atu0218 | | component of type IV pilus | insig. | 100 | 100 |
| o | Rhizobium sp. OAE497 | ABIE40_RS00885 | | CpaF family protein | insig. | 83 | 100 |
| o | Sinorhizobium meliloti 1021 | SMc02820 | | pilus assembly protein | insig. | 87 | 89 |
| o | Bosea sp. OAE506 | ABIE41_RS03685 | | CpaF family protein | insig. | 81 | 87 |
| o | Rhodopseudomonas palustris CGA009 | TX73_019075 | | CpaF family protein | insig. | 73 | 99 |
| o | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS12005 | | CpaF family protein | insig. | 73 | 89 |
| | Sinorhizobium meliloti 1021 | SMa1568 | | CpaF2 pilus assembly protein | insig. | 70 | 97 |
| o | Caulobacter crescentus NA1000 | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 74 | 87 |
| o | Caulobacter crescentus NA1000 Δfur | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 74 | 87 |
| o | Magnetospirillum magneticum AMB-1 | AMB_RS18950 | | CpaF family protein | insig. | 66 | 90 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS08110 | | CpaF family protein | insig. | 50 | 86 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2738 | | type II secretion system protein E (RefSeq) | insig. | 51 | 83 |
| o | Sphingobium sp. HT1-2 | GFF4554 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | cofit | 54 | 83 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS04100 | | pilus assembly protein CpaF | insig. | 50 | 83 |
| o | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0975 | | Flp pilus assembly protein, ATPase CpaF | insig. | 51 | 93 |
| o | Ralstonia solanacearum GMI1000 | RS_RS03280 | | CpaF family protein | insig. | 50 | 83 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS08500 | | CpaF family protein | insig. | 49 | 83 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS22920 | | pilus assembly protein CpaF | insig. | 50 | 83 |
| o | Paraburkholderia sabiae LMG 24235 | QEN71_RS10645 | | CpaF family protein | insig. | 50 | 83 |
| o | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS12510 | | CpaF family protein | insig. | 49 | 83 |
| o | Ralstonia solanacearum UW163 | UW163_RS01055 | | CpaF family protein | insig. | 49 | 83 |
| o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06368 | | pilus assembly protein CpaF | no data | 48 | 87 |
| o | Paraburkholderia graminis OAS925 | ABIE53_004998 | | pilus assembly protein CpaF | sig. | 49 | 83 |
| o | Ralstonia solanacearum PSI07 | RPSI07_RS20760 | | CpaF family protein | insig. | 49 | 84 |
| o | Dinoroseobacter shibae DFL-12 | Dshi_1126 | | type II secretion system protein E (RefSeq) | insig. | 46 | 97 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_01570 | | pilus assembly protein CpaF | insig. | 49 | 90 |
| o | Phaeobacter inhibens DSM 17395 | PGA1_c06110 | | Flp pilus assembly protein, ATPase CpaF | insig. | 51 | 85 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_496 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 51 | 83 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_19980 | | pilus assembly protein CpaF | insig. | 52 | 81 |
| o | Pseudomonas sp. RS175 | PFR28_03044 | | hypothetical protein | insig. | 52 | 81 |
| o | Desulfovibrio vulgaris Hildenborough JW710 | DVU2122 | cpaF | type II/IV secretion system protein (TIGR) | insig. | 47 | 93 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5915 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 51 | 81 |
| o | Variovorax sp. OAS795 | ABID97_RS09105 | | CpaF family protein | insig. | 48 | 84 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2070 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 48 | 90 |
| o | Pseudomonas sp. SVBP6 | COO64_RS23430 | | CpaF family protein | insig. | 51 | 81 |
| | Shewanella amazonensis SB2B | Sama_0708 | | type II/IV secretion system protein E (RefSeq) | insig. | 43 | 93 |
| o | Pseudomonas stutzeri RCH2 | Psest_2619 | | Flp pilus assembly protein, ATPase CpaF | insig. | 49 | 87 |
| o | Pectobacterium carotovorum WPP14 | HER17_RS17705 | | CpaF family protein | insig. | 44 | 96 |
| o | Shewanella loihica PV-4 | Shew_0644 | | FHA domain-containing protein (RefSeq) | insig. | 45 | 89 |
| o | Pseudomonas sp. S08-1 | OH686_02520 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 52 | 81 |
| | Pseudomonas sp. S08-1 | OH686_19900 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 46 | 86 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS34040 | | hypothetical protein | insig. | 46 | 81 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS17490 | | ATPase, T2SS/T4P/T4SS family | insig. | 47 | 80 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS08185 | | pilus assembly protein CpaF | insig. | 44 | 84 |
| | Paraburkholderia graminis OAS925 | ABIE53_002438 | | pilus assembly protein CpaF | cofit | 44 | 84 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS35985 | | pilus assembly protein CpaF | sig. | 43 | 85 |
| | Ralstonia solanacearum GMI1000 | RS_RS22355 | | CpaF family protein | insig. | 46 | 79 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS05045 | | CpaF family protein | insig. | 45 | 82 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS19545 | | CpaF family protein | strong | 47 | 73 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4121 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | no data | 41 | 83 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS18510 | | CpaF family protein | insig. | 45 | 79 |
| | Ralstonia solanacearum UW163 | UW163_RS20620 | | CpaF family protein | insig. | 45 | 79 |
| | Variovorax sp. OAS795 | ABID97_RS24290 | | CpaF family protein | no data | 43 | 82 |
| o | Variovorax sp. SCN45 | GFF2208 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 43 | 82 |
| | Pseudomonas segetis P6 | ACVTMO_RS20025 | | CpaF family protein | insig. | 48 | 70 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_4393 | | type II secretion system protein E | sig. | 44 | 79 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_4393 | | type II secretion system protein E | sig. | 44 | 79 |
| | Pseudomonas orientalis W4I3 | QF045_RS06130 | | CpaF family protein | cofit | 47 | 77 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_13820 | | ATPase | insig. | 47 | 77 |
| | Pseudomonas sp. DMC3 | GFF3341 | | hypothetical protein | cofit | 47 | 77 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS24995 | tadA;inference=COORDINATES | type 4b pilus Flp biogenesis ATPase TadA | insig. | 46 | 77 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_02600 | | type 4b pilus Flp biogenesis ATPase TadA | sig. | 46 | 77 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS57450 | tadA;inference=COORDINATES | type 4b pilus Flp biogenesis ATPase TadA | sig. | 46 | 77 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_1278 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 46 | 79 |
| | Marinobacter adhaerens HP15 | HP15_p187g9 | | type II secretion system protein E | strong | 46 | 73 |
| | Pseudomonas sp. SVBP6 | COO64_RS18465 | | CpaF family protein | insig. | 50 | 69 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS04365 | | ATPase, T2SS/T4P/T4SS family | insig. | 42 | 88 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS03175 | | CpaF family protein | insig. | 48 | 71 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS03175 | | CpaF family protein | strong | 48 | 71 |
| | Pseudomonas simiae WCS417 | PS417_03095 | | ATPase | sig. | 49 | 69 |
| | Mycobacterium tuberculosis H37Rv | Rv3659c | | Conserved hypothetical protein | insig. | 40 | 62 |
| | Methanococcus maripaludis JJ | MMJJ_RS05235 | | Flp pilus assembly complex ATPase component TadA | insig. | 40 | 63 |
| | Methanococcus maripaludis S2 | MMP_RS00250 | | Flp pilus assembly complex ATPase component TadA | strong | 40 | 63 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS43755 | | ATPase, T2SS/T4P/T4SS family | insig. | 37 | 60 |
| | Caulobacter crescentus NA1000 | CCNA_02505 | | type IV secretory pathway, VirB11 ATPase | sig. | 35 | 59 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_02505 | | type IV secretory pathway, VirB11 ATPase | cofit | 35 | 59 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0389 | | P-type conjugative transfer ATPase TrbB | insig. | 38 | 59 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2912 | | P-type conjugative transfer ATPase TrbB | insig. | 38 | 59 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS01870 | | P-type DNA transfer ATPase VirB11 | insig. | 36 | 57 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_3072 | | P-type conjugative transfer ATPase TrbB | insig. | 38 | 59 |
| | Sphingobium sp. HT1-2 | GFF188 | | Conjugative transfer protein TrbB | cofit | 36 | 60 |
| | Marinobacter adhaerens HP15 | HP15_p42g52 | | type II secretion system protein E | cofit | 36 | 61 |
| | Rhodospirillum rubrum S1H | Rru_A0808 | | type II secretion system protein E (NCBI) | insig. | 36 | 58 |
| | Rhodopseudomonas palustris CGA009 | TX73_021370 | | P-type conjugative transfer ATPase TrbB | insig. | 36 | 58 |
| | Methanococcus maripaludis JJ | MMJJ_RS05745 | | type II/IV secretion system ATPase subunit | cofit | 31 | 62 |
| | Methanococcus maripaludis S2 | MMP_RS08625 | | type II/IV secretion system ATPase subunit | cofit | 31 | 62 |
| | Xanthobacter sp. DMC5 | GFF2635 | | hypothetical protein | insig. | 35 | 60 |
| | Rhodopseudomonas palustris CGA009 | TX73_011525 | | P-type conjugative transfer ATPase TrbB | insig. | 34 | 63 |
| | Bifidobacterium breve UCC2003 | BBR_RS10950 | | pilus biosynthesis protein | insig. | 34 | 61 |
| | Agrobacterium fabrum C58 | Atu6041 | | conjugal transfer protein | insig. | 34 | 59 |
| | Xanthobacter sp. DMC5 | GFF3638 | | hypothetical protein | insig. | 33 | 60 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4435 | | Conjugative transfer protein TrbB | insig. | 38 | 51 |
| | Sinorhizobium meliloti 1021 | SMa1302 | | VirB11-like ATPase | insig. | 32 | 58 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2572 | | P-type conjugative transfer ATPase TrbB | insig. | 33 | 58 |
| | Acinetobacter radioresistens SK82 | MPMX26_03199 | | Type IV secretion system protein VirB11 | insig. | 30 | 55 |
| | Rhodopseudomonas palustris CGA009 | TX73_021310 | | P-type conjugative transfer ATPase TrbB | insig. | 34 | 50 |
| | Dickeya dianthicola 67-19 | HGI48_RS08420 | | P-type conjugative transfer ATPase TrbB | insig. | 34 | 60 |
| | Agrobacterium fabrum C58 | Atu5172 | | type IV secretion protein AvhB11 | insig. | 30 | 58 |
| | Dickeya dadantii 3937 | DDA3937_RS03995 | | P-type conjugative transfer ATPase TrbB | insig. | 35 | 60 |
| | Dechlorosoma suillum PS | Dsui_2579 | | Flp pilus assembly protein, ATPase CpaF | cofit | 30 | 62 |
| | Shewanella sp. ANA-3 | Shewana3_1282 | | type II secretion system protein E (RefSeq) | cofit | 38 | 51 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS00155 | | P-type conjugative transfer ATPase TrbB | insig. | 34 | 61 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11415 | | P-type DNA transfer ATPase VirB11 | insig. | 27 | 61 |
| | Variovorax sp. SCN45 | GFF4849 | | Conjugative transfer protein TrbB | insig. | 37 | 51 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c22920 | | putative type IV secretion system protein VirB11 | insig. | 36 | 44 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4176 | | Conjugative transfer protein TrbB | insig. | 38 | 51 |
| | Variovorax sp. SCN45 | GFF4414 | | Conjugative transfer protein TrbB | insig. | 38 | 51 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_12825 | | P-type conjugative transfer ATPase TrbB | no data | 38 | 51 |
| | Ralstonia solanacearum GMI1000 | RS_RS12955 | | P-type conjugative transfer ATPase TrbB | insig. | 34 | 59 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3983 | | type II secretion system protein E (RefSeq) | insig. | 32 | 57 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS03320 | | P-type DNA transfer ATPase VirB11 | insig. | 30 | 57 |
| | Variovorax sp. SCN45 | GFF6579 | | Conjugative transfer protein TrbB | insig. | 37 | 51 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF3453 | | Conjugative transfer protein TrbB | insig. | 37 | 51 |
| | Dickeya dianthicola 67-19 | HGI48_RS03925 | | P-type conjugative transfer ATPase TrbB | insig. | 36 | 51 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3648 | | type II secretion system protein E (RefSeq) | insig. | 30 | 57 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.2046.1 | | hypothetical protein | insig. | 27 | 60 |
| | Escherichia coli ECOR38 | HEPCGN_00330 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 27 | 56 |
| | Ralstonia solanacearum UW163 | UW163_RS23875 | | P-type DNA transfer ATPase VirB11 | insig. | 32 | 46 |
| | Agrobacterium fabrum C58 | Atu6177 | | component of type IV secretion system | insig. | 28 | 55 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS36345 | | hypothetical protein | insig. | 27 | 55 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS00810 | | type IV secretion system protein VirB11 | insig. | 26 | 56 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS06740 | | P-type DNA transfer ATPase VirB11 | insig. | 25 | 57 |
| | Escherichia coli ECOR27 | NOLOHH_00295 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 27 | 58 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS32155 | | type IV secretion system protein VirB11 | sig. | 27 | 57 |
| | Escherichia coli ECOR27 | NOLOHH_00210 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 27 | 55 |
| | Dickeya dianthicola ME23 | DZA65_RS14430 | | P-type DNA transfer ATPase VirB11 | insig. | 26 | 52 |
| | Azospirillum brasilense Sp245 | AZOBR_RS33110 | | hypothetical protein | insig. | 29 | 51 |
| | Dickeya dadantii 3937 | DDA3937_RS13875 | | P-type DNA transfer ATPase VirB11 | insig. | 27 | 56 |
| | Dickeya dianthicola 67-19 | HGI48_RS07090 | | P-type DNA transfer ATPase VirB11 | insig. | 27 | 56 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS40910 | | P-type DNA transfer ATPase VirB11 | no data | 28 | 47 |
| | Methylophilus sp. DMC18 | GFF275 | | hypothetical protein | insig. | 30 | 43 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS17980 | | general secretion pathway protein GspE | insig. | 35 | 31 |
| | Dechlorosoma suillum PS | Dsui_1320 | | type IV-A pilus assembly ATPase PilB | insig. | 26 | 65 |
| | Xanthobacter sp. DMC5 | GFF3108 | | Type II secretion system protein E | insig. | 33 | 37 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_4766 | | Type IV fimbrial assembly, ATPase PilB | insig. | 29 | 43 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS06180 | | type IV-A pilus assembly ATPase PilB | sig. | 34 | 31 |
| | Ralstonia solanacearum UW163 | UW163_RS07445 | | type IV-A pilus assembly ATPase PilB | insig. | 34 | 31 |
| | Ralstonia solanacearum GMI1000 | RS_RS14130 | | type IV-A pilus assembly ATPase PilB | insig. | 34 | 31 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS11060 | | type IV-A pilus assembly ATPase PilB | cofit | 34 | 31 |
| | Lysobacter sp. OAE881 | ABIE51_RS13600 | | GspE/PulE family protein | insig. | 30 | 44 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS01845 | | type IV-A pilus assembly ATPase PilB | strong | 34 | 31 |
| | Variovorax sp. SCN45 | GFF2510 | | Type IV fimbrial assembly, ATPase PilB | strong | 30 | 43 |
| | Shewanella sp. ANA-3 | Shewana3_3660 | | type II secretion system protein E (RefSeq) | insig. | 25 | 74 |
| | Variovorax sp. OAS795 | ABID97_RS23430 | | type IV-A pilus assembly ATPase PilB | strong | 30 | 43 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1279 | | Type IV fimbrial assembly, ATPase PilB | cofit | 30 | 43 |
| | Rahnella sp. WP5 | EX31_RS16170 | | type II secretion system ATPase GspE | insig. | 26 | 55 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_13785 | | type II secretion system protein GspE | no data | 25 | 61 |
| | Acinetobacter baumannii LAC-4 | RR41_RS15895 | | type II secretion system ATPase GspE | sig. | 26 | 50 |
| | Dickeya dianthicola 67-19 | HGI48_RS14730 | | type II secretion system ATPase GspE | insig. | 27 | 54 |
| | Shewanella sp. ANA-3 | Shewana3_0152 | | type II secretion system protein E (GspE) (RefSeq) | strong | 26 | 55 |
| | Acinetobacter radioresistens SK82 | MPMX26_00578 | | Type II secretion system protein E | insig. | 26 | 50 |
| | Dickeya dadantii 3937 | DDA3937_RS14795 | | type II secretion system ATPase GspE | cofit | 27 | 54 |
| | Pseudomonas sp. BP01 | JOY50_RS06170 | | GspE/PulE family protein | insig. | 36 | 24 |
| | Methylophilus sp. DMC18 | GFF865 | | Twitching mobility protein | insig. | 26 | 54 |
| | Klebsiella pneumoniae MRSN742743 | KFA93_RS11785 | gspE;go_component=type | type II secretion system ATPase GspE | insig. | 26 | 56 |
| | Shewanella oneidensis MR-1 | SO0167 | gspE | general secretion pathway protein E (NCBI ptt file) | strong | 25 | 55 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS30925 | | GspE/PulE family protein | insig. | 37 | 24 |
| | Dickeya dianthicola ME23 | DZA65_RS15675 | | type II secretion system ATPase GspE | cofit | 27 | 54 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_13340 | | type II secretion system ATPase GspE | insig. | 26 | 56 |
| | Pseudomonas putida KT2440 | PP_3483 | | putative Type II secretion system protein | insig. | 36 | 24 |
| | Marinobacter adhaerens HP15 | HP15_2409 | | ATPase, type IV, pilus assembly, PilB | cofit | 32 | 37 |
| | Klebsiella michiganensis M5al | BWI76_RS05085 | | general secretion pathway protein GspE | insig. | 26 | 56 |
| | Shewanella amazonensis SB2B | Sama_0369 | | type IV pilus biogenesis protein PilB (RefSeq) | insig. | 26 | 47 |
| | Shewanella sp. ANA-3 | Shewana3_0419 | | type IV-A pilus assembly ATPase PilB (RefSeq) | sig. | 25 | 47 |
| | Shewanella oneidensis MR-1 | SO4109 | mshE | MSHA biogenesis protein MshE (NCBI ptt file) | strong | 24 | 74 |
| | Shewanella oneidensis MR-1 | SO0416 | | type IV pilus biogenesis protein PilB (NCBI ptt file) | insig. | 25 | 47 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_09340 | | type II secretion system protein GspE | insig. | 31 | 33 |
| | Alteromonas macleodii MIT1002 | MIT1002_03060 | | Twitching mobility protein | insig. | 27 | 55 |
| | Pseudomonas sp. S08-1 | OH686_00770 | | Twitching motility protein PilT | insig. | 33 | 26 |
| | Acinetobacter radioresistens SK82 | MPMX26_00246 | | hypothetical protein | cofit | 27 | 45 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_3847 | | Predicted secretion system X protein GspE-like | insig. | 36 | 24 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_4473 | | Twitching motility protein PilT | strong | 29 | 41 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_2138 | | type IV-A pilus assembly ATPase PilB | strong | 27 | 43 |
| | Methylophilus sp. DMC18 | GFF6 | | Type II secretion system protein E | insig. | 37 | 24 |
| | Escherichia coli BL21 | ECD_02837 | | putative type II secretion protein GspE | strong | 25 | 62 |
| | Escherichia fergusonii Becca | EFB2_00846 | | Type II secretion system protein E | insig. | 25 | 62 |
| | Escherichia coli ECOR27 | NOLOHH_10980 | gspE | general secretion pathway protein E | sig. | 25 | 62 |
| | Escherichia coli ECOR38 | HEPCGN_18055 | gspE | general secretion pathway protein E | strong | 25 | 62 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF66 | | Predicted secretion system X protein GspE-like | no data | 27 | 49 |
| | Methylophilus sp. DMC18 | GFF1853 | | Type II secretion system protein E | insig. | 36 | 24 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS04100 | | type II/IV secretion system protein | insig. | 34 | 30 |
| | Ralstonia solanacearum UW163 | UW163_RS09570 | | type II/IV secretion system protein | insig. | 34 | 30 |
| | Dechlorosoma suillum PS | Dsui_0605 | | pilus retraction protein PilT | insig. | 33 | 24 |
| | Acinetobacter baumannii LAC-4 | RR41_RS17340 | | type IV-A pilus assembly ATPase PilB | insig. | 27 | 42 |
| | Ralstonia solanacearum GMI1000 | RS_RS11580 | | type II/IV secretion system protein | insig. | 36 | 24 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS22670 | | type II/IV secretion system protein | insig. | 35 | 24 |
| | Ralstonia solanacearum UW163 | UW163_RS23265 | | type II/IV secretion system protein | insig. | 35 | 24 |
| | Pseudomonas segetis P6 | ACVTMO_RS11710 | | PilT/PilU family type 4a pilus ATPase | insig. | 32 | 26 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.1713.1 | | Twitching motility protein PilT | sig. | 31 | 31 |
| | Mucilaginibacter yixingensis YX-36 DSM 26809 | ABZR88_RS02570 | | GspE/PulE family protein | insig. | 29 | 37 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS13950 | | GspE/PulE family protein | insig. | 35 | 24 |
| | Ralstonia solanacearum GMI1000 | RS_RS17860 | | type II/IV secretion system protein | no data | 35 | 24 |
| | Shewanella amazonensis SB2B | Sama_2475 | | twitching motility protein PilU (RefSeq) | insig. | 32 | 25 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_265 | | General secretion pathway protein E | insig. | 31 | 33 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_3745 | | General secretion pathway protein E | insig. | 31 | 33 |
| | Shewanella loihica PV-4 | Shew_3438 | | type IV-A pilus assembly ATPase PilB (RefSeq) | strong | 25 | 47 |
| | Ralstonia solanacearum GMI1000 | RS_RS19385 | | type II/IV secretion system protein | insig. | 33 | 31 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_2906 | | Pilus retraction protein PilT | sig. | 32 | 26 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_2906 | | Pilus retraction protein PilT | insig. | 32 | 26 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_12550 | | type II secretion system protein GspE | insig. | 31 | 33 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_02465 | | twitching motility protein PilT | insig. | 30 | 25 |
| | Shewanella oneidensis MR-1 | SO3351 | pilT | twitching motility protein PilT (NCBI ptt file) | strong | 30 | 38 |
| | Pseudomonas simiae WCS417 | PS417_26720 | | twitching motility protein PilT | insig. | 30 | 30 |
| | Pectobacterium carotovorum WPP14 | HER17_RS06690 | | type II secretion system ATPase GspE | insig. | 28 | 43 |
| | Variovorax sp. SCN45 | GFF3743 | | Twitching motility protein PilT | strong | 27 | 41 |
| | Pseudomonas segetis P6 | ACVTMO_RS03030 | | PilT/PilU family type 4a pilus ATPase | sig. | 32 | 32 |
| | Escherichia coli ECRC100 | OKFHMN_00280 | gspE | type II secretion system ATPase GspE | insig. | 28 | 33 |
| | Escherichia coli ECRC101 | MCAODC_15270 | gspE | type II secretion system ATPase GspE | insig. | 28 | 33 |
| | Escherichia coli ECRC102 | NIAGMN_28600 | gspE | type II secretion system ATPase GspE | cofit | 28 | 33 |
| | Escherichia coli ECRC98 | JDDGAC_30300 | gspE | type II secretion system ATPase GspE | insig. | 28 | 33 |
| | Variovorax sp. SCN45 | GFF5234 | | General secretion pathway protein E / Type II secretion cytoplasmic ATP binding protein (PulE, ATPase) | insig. | 26 | 49 |
| | Pseudomonas sp. S08-1 | OH686_14715 | | MSHA biogenesis protein MshE | insig. | 26 | 50 |
| | Lysobacter sp. OAE881 | ABIE51_RS16055 | | type IV pilus twitching motility protein PilT | strong | 24 | 69 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS10420 | | type IV pilus twitching motility protein PilT | sig. | 30 | 31 |
| | Acinetobacter radioresistens SK82 | MPMX26_02406 | | Twitching mobility protein | insig. | 29 | 33 |
| | Pseudomonas simiae WCS417 | PS417_18085 | | general secretion pathway protein GspE | insig. | 28 | 49 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00740 | | Type II secretion system protein E | cofit | 25 | 48 |
| | Shewanella oneidensis MR-1 | SO3350 | pilU | twitching motility protein PilU (NCBI ptt file) | strong | 32 | 25 |
| | Pseudomonas sp. S08-1 | OH686_10965 | | Type IV pilus assembly ATPase component PilU | insig. | 33 | 24 |
| | Paraburkholderia graminis OAS925 | ABIE53_003594 | | type IV pilus assembly protein PilB | insig. | 32 | 24 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS27305 | | ATPase, T2SS/T4P/T4SS family | insig. | 32 | 24 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS05565 | | twitching motility protein PilT | insig. | 32 | 24 |
| | Ralstonia solanacearum UW163 | UW163_RS08060 | | twitching motility protein PilT | insig. | 32 | 24 |
| | Ralstonia solanacearum GMI1000 | RS_RS13425 | | twitching motility protein PilT | insig. | 32 | 24 |
| | Shewanella sp. ANA-3 | Shewana3_1196 | | twitching motility protein (RefSeq) | insig. | 32 | 25 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS19940 | | type II secretion system ATPase GspE | insig. | 29 | 33 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS19940 | | type II secretion system ATPase GspE | insig. | 29 | 33 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_03878 | | Type II secretion system protein E | cofit | 29 | 33 |
| | Acinetobacter baumannii LAC-4 | RR41_RS14800 | | type IV pilus twitching motility protein PilT | insig. | 29 | 34 |
| | Erwinia tracheiphila SCR3 | LU632_RS25235 | gspE | type II secretion system ATPase GspE | insig. | 26 | 43 |
| | Shewanella amazonensis SB2B | Sama_0170 | | general secretion pathway protein E (RefSeq) | strong | 24 | 55 |
| | Alteromonas macleodii MIT1002 | MIT1002_03977 | | Type II traffic warden ATPase | strong | 24 | 55 |
| | Synechococcus elongatus PCC 7942 | Synpcc7942_2070 | pilT | twitching motility protein | strong | 29 | 48 |
| | Marinobacter adhaerens HP15 | HP15_416 | | type IV-A pilus assembly ATPase PilB | cofit | 26 | 54 |
| | Variovorax sp. OAS795 | ABID97_RS06945 | | ATPase, T2SS/T4P/T4SS family | insig. | 26 | 49 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_09695 | gspE | type II secretion system ATPase GspE | cofit | 26 | 43 |
| | Synechocystis sp000284455 PCC 6803 | SGL_RS11545 | | type IV pilus twitching motility protein PilT | no data | 30 | 35 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS28365 | | type IV pilus twitching motility protein PilT | insig. | 30 | 30 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS28365 | | type IV pilus twitching motility protein PilT | insig. | 30 | 30 |
| | Shewanella sp. ANA-3 | Shewana3_1195 | | pilus retraction ATPase PilT (RefSeq) | cofit | 29 | 38 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_13070 | | type II secretion system protein GspE | sig. | 28 | 45 |
| | Klebsiella pneumoniae MRSN742743 | KFA93_RS11525 | gspE;inference=COORDINATES | type II secretion system protein GspE | insig. | 28 | 45 |
| | Escherichia coli BL21 | ECD_03177 | | general secretory pathway component, cryptic | insig. | 26 | 46 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00377 | | Putative type II secretion system protein E | insig. | 26 | 46 |
| | Escherichia coli BW25113 | b3326 | gspE | general secretory pathway component, cryptic (NCBI) | insig. | 26 | 46 |
| | Pseudomonas sp. S08-1 | OH686_16505 | | type IV-A pilus assembly ATPase PilB | insig. | 32 | 24 |
| | Alteromonas macleodii MIT1002 | MIT1002_03059 | | Twitching mobility protein | insig. | 31 | 26 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1031 | | twitching motility protein (RefSeq) | sig. | 31 | 36 |
| | Escherichia fergusonii Becca | EFB2_00497 | | Putative type II secretion system protein E | cofit | 26 | 46 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS19065 | | type II secretion system ATPase GspE | insig. | 26 | 46 |