| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Xanthobacter sp. DMC5 | GFF2676 | | tRNA U34 carboxymethyltransferase | insig. | 100 | 100 |
| o | Bosea sp. OAE506 | ABIE41_RS01025 | | TIGR04290 family methyltransferase | insig. | 65 | 91 |
| o | Rhizobium sp. OAE497 | ABIE40_RS30980 | | TIGR04290 family methyltransferase | insig. | 61 | 95 |
| o | Sinorhizobium meliloti 1021 | SM_b20238 | | hypothetical protein | insig. | 61 | 92 |
| o | Lysobacter sp. OAE881 | ABIE51_RS07470 | | TIGR04290 family methyltransferase | insig. | 53 | 92 |
| o | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_12105 | | methyltransferase, TIGR04290 family | insig. | 48 | 87 |
| o | Variovorax sp. OAS795 | ABID97_RS11915 | | TIGR04290 family methyltransferase | insig. | 52 | 90 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3550 | | ubiquinone biosynthesis O-methyltransferase (RefSeq) | no data | 37 | 45 |
| | Pseudomonas sp. SVBP6 | COO64_RS16610 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 28 | 86 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_03325 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 29 | 85 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_826 | | tRNA (5-methoxyuridine) 34 synthase | insig. | 28 | 86 |
| | Pseudomonas putida KT2440 | PP_1440 | | tRNA (mo5U34)-methyltransferase | strong | 28 | 86 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS05290 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 27 | 86 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS05290 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | sig. | 27 | 86 |
| | Pseudomonas sp. RS175 | PFR28_00318 | | tRNA U34 carboxymethyltransferase | sig. | 28 | 86 |
| | Pseudomonas sp. DMC3 | GFF4316 | | tRNA U34 carboxymethyltransferase | insig. | 28 | 85 |
| | Pseudomonas stutzeri RCH2 | Psest_3085 | | methyltransferase, putative | sig. | 28 | 86 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_4812 | | tRNA (5-methoxyuridine) 34 synthase | insig. | 28 | 86 |
| | Pseudomonas segetis P6 | MPMX49_01414 | | tRNA U34 carboxymethyltransferase | insig. | 29 | 86 |
| | Pseudomonas sp. BP01 | JOY50_RS14730 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 27 | 86 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01250 | | tRNA U34 carboxymethyltransferase | cofit | 26 | 86 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_16015 | | tRNA (mo5U34)-methyltransferase | insig. | 28 | 86 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_3158 | | tRNA (5-methoxyuridine) 34 synthase | sig. | 28 | 86 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_06450 | | tRNA (mo5U34)-methyltransferase | sig. | 28 | 86 |
| | Marinobacter adhaerens HP15 | HP15_2493 | | methyltransferase | sig. | 34 | 52 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_3947 | | Methyltransferase, putative | strong | 28 | 72 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3947 | | Methyltransferase, putative | insig. | 28 | 72 |
| | Pseudomonas simiae WCS417 | PS417_05220 | | tRNA methyltransferase | insig. | 28 | 72 |
| | Acinetobacter radioresistens SK82 | MPMX26_00034 | | Ubiquinone biosynthesis O-methyltransferase | no data | 33 | 49 |
| | Rhizobium sp. OAE497 | ABIE40_RS17330 | | bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG | no data | 42 | 33 |
| | Pseudomonas sp. S08-1 | OH686_05375 | | tRNA (mo5U34)-methyltransferase | cofit | 27 | 71 |
| | Shewanella loihica PV-4 | Shew_2082 | | putative methyltransferase (RefSeq) | strong | 28 | 87 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_05895 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 29 | 71 |
| | Sinorhizobium meliloti 1021 | SMc02440 | | 3-demethylubiquinone-9 3-methyltransferase | no data | 43 | 32 |
| | Azospirillum sp. SherDot2 | MPMX19_02672 | | Ubiquinone biosynthesis O-methyltransferase | no data | 32 | 45 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_25460 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 29 | 71 |
| | Bosea sp. OAE506 | ABIE41_RS20085 | | bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG | no data | 41 | 32 |
| | Klebsiella michiganensis M5al | BWI76_RS18195 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | sig. | 28 | 71 |
| | Rhodospirillum rubrum S1H | Rru_A0742 | | Ubiquinone biosynthesis O-methyltransferase (NCBI) | no data | 39 | 44 |
| | Shewanella sp. ANA-3 | Shewana3_1950 | | putative methyltransferase (RefSeq) | strong | 34 | 47 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_22090 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | strong | 28 | 71 |
| | Shewanella amazonensis SB2B | Sama_1846 | | methyltransferase, putative (RefSeq) | strong | 32 | 49 |
| | Enterobacter sp. TBS_079 | MPMX20_02776 | | tRNA U34 carboxymethyltransferase | strong | 30 | 62 |
| | Erwinia tracheiphila SCR3 | LU632_RS13460 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | strong | 28 | 71 |
| | Shewanella oneidensis MR-1 | SO2436 | | methyltransferase, putative (NCBI ptt file) | strong | 34 | 47 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF1652 | | tRNA (5-methoxyuridine) 34 synthase | insig. | 30 | 62 |
| | Alteromonas macleodii MIT1002 | MIT1002_02226 | | tRNA (mo5U34)-methyltransferase | strong | 27 | 72 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_0686 | | tRNA (mo5U34)-methyltransferase | insig. | 33 | 47 |
| | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS02285 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | cofit | 28 | 71 |
| | Erwinia amylovora T8 | OLJFJH_12815 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | cofit | 33 | 50 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_1515 | | methyltransferase, putative | cofit | 30 | 54 |
| | Azospirillum sp. SherDot2 | MPMX19_06613 | | Ubiquinone biosynthesis O-methyltransferase, mitochondrial | cofit | 40 | 36 |
| | Escherichia fergusonii Becca | EFB2_02071 | | tRNA U34 carboxymethyltransferase | insig. | 32 | 47 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS10700 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | no data | 32 | 47 |
| | Dickeya dianthicola 67-19 | HGI48_RS08095 | | class I SAM-dependent methyltransferase | insig. | 33 | 33 |
| | Escherichia coli BW25113 | b1871 | yecP | predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) | strong | 32 | 47 |
| | Escherichia coli BL21 | ECD_01842 | | tRNA (cmo5U34)-carboxymethyltransferase, carboxy-SAM-dependent | insig. | 32 | 47 |
| | Escherichia coli ECOR27 | NOLOHH_16665 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 32 | 47 |
| | Escherichia coli ECOR38 | HEPCGN_04630 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | cofit | 32 | 47 |
| | Escherichia coli ECRC100 | OKFHMN_26220 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 32 | 47 |
| | Escherichia coli ECRC101 | MCAODC_19185 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 32 | 47 |
| | Escherichia coli ECRC102 | NIAGMN_01840 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 32 | 47 |
| | Escherichia coli ECRC62 | BNILDI_15455 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 32 | 47 |
| | Escherichia coli ECRC98 | JDDGAC_00795 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | insig. | 32 | 47 |
| | Escherichia coli ECRC99 | KEDOAH_01885 | cmoB | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | no data | 32 | 47 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01857 | | tRNA U34 carboxymethyltransferase | insig. | 32 | 47 |
| | Azospirillum brasilense Sp245 | AZOBR_RS18885 | | 3-demethylubiquinone-9 3-methyltransferase | no data | 40 | 32 |
| | Agrobacterium fabrum C58 | Atu3508 | | 3-demethylubiquinone-9 3-methyltransferase | no data | 38 | 32 |
| | Rhodopseudomonas palustris CGA009 | TX73_003120 | | bifunctional 3-demethylubiquinone 3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol methylase | no data | 38 | 40 |
| | Pantoea sp. MT58 | IAI47_08405 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | strong | 28 | 71 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS06665 | | class I SAM-dependent rRNA methyltransferase | insig. | 36 | 33 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS06920 | | class I SAM-dependent rRNA methyltransferase | insig. | 36 | 33 |
| | Dickeya dianthicola ME23 | DZA65_RS08690 | | methyltransferase domain-containing protein | insig. | 35 | 33 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS14495 | | bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG | no data | 36 | 33 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_2319 | | 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64) | no data | 36 | 42 |
| | Methylophilus sp. DMC18 | GFF1234 | | Ubiquinone biosynthesis O-methyltransferase | no data | 39 | 33 |
| | Pseudomonas simiae WCS417 | PS417_08100 | | 3-demethylubiquinone-9 3-methyltransferase | no data | 37 | 34 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS08070 | | bifunctional 3-demethylubiquinone 3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol methylase | no data | 37 | 34 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS08070 | | bifunctional 3-demethylubiquinone 3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol methylase | no data | 37 | 34 |
| | Pseudomonas sp. DMC3 | GFF4682 | | Ubiquinone biosynthesis O-methyltransferase | no data | 37 | 34 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01756 | | Ubiquinone biosynthesis O-methyltransferase | no data | 37 | 34 |
| | Pseudomonas segetis P6 | MPMX49_02904 | | Ubiquinone biosynthesis O-methyltransferase | no data | 38 | 33 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_3650 | | 3-demethylubiquinone-9 3-methyltransferase | no data | 38 | 33 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3650 | | 3-demethylubiquinone-9 3-methyltransferase | no data | 38 | 33 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_02095 | | 3-demethylubiquinone-9 3-methyltransferase | no data | 38 | 33 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_29590 | | SAM-dependent methyltransferase | insig. | 38 | 31 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2171 | | 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64) | no data | 38 | 33 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_356 | | 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64) | no data | 38 | 33 |
| | Pseudomonas sp. BP01 | JOY50_RS16160 | ubiG | bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG | no data | 37 | 34 |
| | Shewanella amazonensis SB2B | Sama_1730 | | 3-demethylubiquinone-9 3-methyltransferase (RefSeq) | no data | 31 | 47 |
| | Rahnella sp. WP5 | EX31_RS17290 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | sig. | 30 | 49 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_16115 | | bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG | no data | 39 | 33 |
| | Xanthobacter sp. DMC5 | GFF100 | | Ubiquinone biosynthesis O-methyltransferase | no data | 38 | 42 |
| | Pseudomonas sp. RS175 | PFR28_00901 | | Ubiquinone biosynthesis O-methyltransferase | no data | 38 | 33 |
| | Pseudomonas putida KT2440 | PP_1765 | | Ubiquinone biosynthesis O-methyltransferase | no data | 38 | 33 |
| | Serratia liquefaciens MT49 | IAI46_14675 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | sig. | 32 | 48 |
| | Marinobacter adhaerens HP15 | HP15_1227 | | 3-demethylubiquinone-9 3-methyltransferase | no data | 36 | 33 |
| | Pseudomonas stutzeri RCH2 | Psest_2002 | | ubiquinone biosynthesis O-methyltransferase | no data | 41 | 33 |
| | Agrobacterium fabrum C58 | Atu1041 | | methyltransferase | insig. | 36 | 37 |
| | Pectobacterium carotovorum WPP14 | HER17_RS11890 | | tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB | sig. | 33 | 47 |