| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_06560 | | 6-carboxytetrahydropterin synthase QueD | insig. | 100 | 100 |
| o | Erwinia amylovora T8 | OLJFJH_09295 | | 6-carboxytetrahydropterin synthase QueD | insig. | 82 | 98 |
| o | Enterobacter sp. TBS_079 | MPMX20_03644 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | sig. | 82 | 98 |
| o | Klebsiella pneumoniae MKP103 | KDGMDA_03305 | | 6-carboxytetrahydropterin synthase QueD | insig. | 80 | 99 |
| o | Enterobacter asburiae PDN3 | EX28DRAFT_2854 | | queuosine biosynthesis protein QueD | insig. | 83 | 98 |
| o | Escherichia coli ECOR38 | HEPCGN_19100 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 81 | 99 |
| o | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS16820 | | 6-carboxytetrahydropterin synthase QueD | cofit | 83 | 98 |
| o | Klebsiella michiganensis M5al | BWI76_RS22775 | | 6-carboxytetrahydropterin synthase QueD | insig. | 81 | 98 |
| o | Pantoea sp. MT58 | IAI47_04145 | | 6-carboxytetrahydropterin synthase QueD | insig. | 83 | 98 |
| o | Dickeya dianthicola 67-19 | HGI48_RS17030 | | 6-carboxytetrahydropterin synthase QueD | insig. | 82 | 98 |
| o | Escherichia coli BL21 | ECD_02610 | | 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) | sig. | 79 | 99 |
| o | Escherichia fergusonii Becca | EFB2_01152 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 79 | 99 |
| o | Escherichia coli ECOR27 | NOLOHH_12180 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 79 | 99 |
| o | Escherichia coli ECRC100 | OKFHMN_20365 | queD | 6-carboxytetrahydropterin synthase QueD | cofit | 79 | 99 |
| o | Escherichia coli ECRC101 | MCAODC_10890 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 79 | 99 |
| o | Escherichia coli ECRC102 | NIAGMN_18135 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 79 | 99 |
| o | Escherichia coli ECRC62 | BNILDI_10730 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 79 | 99 |
| o | Escherichia coli ECRC98 | JDDGAC_24030 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 79 | 99 |
| o | Escherichia coli ECRC99 | KEDOAH_07760 | queD | 6-carboxytetrahydropterin synthase QueD | no data | 79 | 99 |
| o | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00968 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 79 | 99 |
| o | Escherichia coli BW25113 | b2765 | ygcM | 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) | sig. | 79 | 99 |
| o | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_17415 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 81 | 98 |
| o | Erwinia tracheiphila SCR3 | LU632_RS06395 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 81 | 98 |
| o | Dickeya dianthicola ME23 | DZA65_RS18105 | | 6-carboxytetrahydropterin synthase QueD | sig. | 80 | 98 |
| o | Escherichia coli Nissle 1917 | ECOLIN_RS15415 | | 6-carboxytetrahydropterin synthase QueD | insig. | 79 | 98 |
| o | Dickeya dadantii 3937 | DDA3937_RS16965 | | 6-carboxytetrahydropterin synthase QueD | sig. | 79 | 98 |
| o | Rahnella sp. WP5 | EX31_RS07915 | | 6-carboxytetrahydropterin synthase QueD | insig. | 78 | 98 |
| o | Pectobacterium carotovorum WPP14 | HER17_RS04205 | | 6-carboxytetrahydropterin synthase QueD | insig. | 77 | 98 |
| o | Pseudomonas segetis P6 | MPMX49_02208 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 79 | 96 |
| o | Serratia liquefaciens MT49 | IAI46_03680 | | 6-carboxytetrahydropterin synthase QueD | insig. | 76 | 98 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_13390 | | 6-carboxytetrahydropterin synthase QueD | no data | 76 | 96 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS10415 | | 6-carboxytetrahydropterin synthase QueD | insig. | 74 | 96 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS10415 | | 6-carboxytetrahydropterin synthase QueD | sig. | 74 | 96 |
| o | Pseudomonas simiae WCS417 | PS417_09830 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | sig. | 74 | 96 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_02161 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | cofit | 74 | 96 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_110 | | Queuosine biosynthesis QueD, PTPS-I | insig. | 73 | 97 |
| o | Pseudomonas sp. RS175 | PFR28_02851 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 73 | 96 |
| o | Lysobacter sp. OAE881 | ABIE51_RS17950 | | 6-carboxytetrahydropterin synthase QueD | sig. | 73 | 95 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_21260 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 72 | 97 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_37 | | 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I | strong | 72 | 97 |
| o | Pseudomonas sp. DMC3 | GFF1361 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | cofit | 72 | 97 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_19760 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 72 | 97 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2447 | | 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I | sig. | 72 | 97 |
| o | Pseudomonas putida KT2440 | PP_2341 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | sig. | 73 | 96 |
| o | Pseudomonas syringae pv. syringae B728a | Psyr_3215 | | 6-pyruvoyl tetrahydropterin synthase and hypothetical protein | no data | 73 | 96 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3215 | | 6-pyruvoyl tetrahydropterin synthase and hypothetical protein | no data | 73 | 96 |
| o | Pseudomonas sp. BP01 | JOY50_RS10905 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 72 | 96 |
| o | Pseudomonas stutzeri RCH2 | Psest_2010 | | queuosine biosynthesis protein QueD | no data | 70 | 97 |
| o | Pseudomonas sp. SVBP6 | COO64_RS17240 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 72 | 96 |
| o | Pseudomonas sp. S08-1 | OH686_00030 | | queuosine biosynthesis protein QueD | insig. | 76 | 96 |
| o | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.4528.1 | | hypothetical protein | insig. | 70 | 95 |
| o | Rhodanobacter denitrificans MT42 | LRK55_RS09085 | | 6-carboxytetrahydropterin synthase QueD | insig. | 64 | 95 |
| o | Rhodanobacter sp. FW510-T8 | OKGIIK_15925 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 65 | 90 |
| o | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS13650 | | 6-carboxytetrahydropterin synthase QueD | cofit | 67 | 90 |
| o | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS02150 | | 6-carboxytetrahydropterin synthase QueD | sig. | 65 | 90 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS09315 | | 6-carboxytetrahydropterin synthase QueD | insig. | 65 | 90 |
| o | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS09655 | | 6-carboxytetrahydropterin synthase QueD | strong | 65 | 90 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF4822 | | Queuosine biosynthesis QueD, PTPS-I | strong | 78 | 70 |
| o | Desulfovibrio vulgaris Hildenborough JW710 | DVU2657 | | 6-pyruvoyl tetrahydrobiopterin synthase, putative (TIGR) | insig. | 57 | 95 |
| o | Desulfovibrio vulgaris Miyazaki F | DvMF_1123 | | queuosine biosynthesis protein QueD (RefSeq) | no data | 53 | 96 |
| o | Xanthobacter sp. DMC5 | GFF3475 | | hypothetical protein | insig. | 55 | 89 |
| o | Rhodopseudomonas palustris CGA009 | TX73_008525 | | 6-carboxytetrahydropterin synthase QueD | insig. | 53 | 96 |
| o | Synechocystis sp000284455 PCC 6803 | SGL_RS15290 | | 6-carboxytetrahydropterin synthase QueD | strong | 50 | 93 |
| o | Agrobacterium fabrum C58 | Atu3345 | | 6-pyruvoyl tetrahydrobiopterin synthase | insig. | 39 | 93 |
| o | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS08690 | | 6-carboxytetrahydropterin synthase | no data | 40 | 96 |
| o | Caulobacter crescentus NA1000 | CCNA_03260 | | queuosine biosynthesis protein QueD | strong | 41 | 89 |
| o | Caulobacter crescentus NA1000 Δfur | CCNA_03260 | | queuosine biosynthesis protein QueD | cofit | 41 | 89 |
| o | Sinorhizobium meliloti 1021 | SM_b20939 | | 6-pyruvoyl-tetrahydropterin synthase | insig. | 36 | 97 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4632 | | 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I | no data | 36 | 88 |
| o | Variovorax sp. OAS795 | ABID97_RS07420 | | 6-carboxytetrahydropterin synthase | insig. | 33 | 94 |
| o | Phaeobacter inhibens DSM 17395 | PGA1_c05610 | | putative queuosine biosynthesis protein QueD | insig. | 35 | 93 |
| o | Methylophilus sp. DMC18 | GFF1366 | | hypothetical protein | insig. | 35 | 88 |
| o | Phocaeicola dorei CL03T12C01 | ABI39_RS10595 | | 6-carboxytetrahydropterin synthase QueD | sig. | 36 | 93 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_0129 | | 6-pyruvoyl tetrahydropterin synthase and hypothetical protein (RefSeq) | no data | 37 | 88 |
| o | Acidovorax sp. GW101-3H11 | Ac3H11_3826 | | 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I | insig. | 31 | 95 |
| o | Variovorax sp. SCN45 | GFF2576 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase (EC 4.1.2.50) | cofit | 31 | 94 |
| o | Phocaeicola vulgatus CL09T03C04 | HMPREF1058_RS14875 | | 6-carboxytetrahydropterin synthase QueD | insig. | 35 | 93 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU1352 | | 6-pyruvoyl tetrahydrobiopterin synthase, putative (TIGR) | no data | 41 | 73 |
| o | Bacteroides stercoris CC31F | HMPREF1181_RS13890 | | 6-carboxytetrahydropterin synthase QueD | cofit | 35 | 93 |
| | Bacteroides thetaiotaomicron VPI-5482 | BT4453 | | 6-pyruvoyl-tetrahydropterin synthase (NCBI ptt file) | strong | 34 | 87 |
| o | Parabacteroides merdae CL09T00C40 | HMPREF1078_RS00755 | | 6-carboxytetrahydropterin synthase QueD | no data | 34 | 95 |
| | Synechococcus elongatus PCC 7942 | Synpcc7942_1184 | | 6-pyruvoyl-tetrahydropterin synthase-like protein | strong | 30 | 98 |
| | Bacteroides stercoris CC31F | HMPREF1181_RS07300 | | 6-carboxytetrahydropterin synthase QueD | insig. | 32 | 87 |
| | Acinetobacter radioresistens SK82 | MPMX26_01230 | | hypothetical protein | strong | 30 | 83 |
| | Synechococcus elongatus PCC 7942 | Synpcc7942_1953 | | 6-pyruvoyl tetrahydrobiopterin synthase | strong | 33 | 97 |
| | Pedobacter sp. GW460-11-11-14-LB5 | CA265_RS21885 | | 6-pyruvoyl tetrahydrobiopterin synthase | strong | 32 | 89 |
| | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_20555 | | 6-pyruvoyl tetrahydrobiopterin synthase | sig. | 31 | 82 |
| | Cellulophaga baltica 18 | M666_RS09275 | | 6-carboxytetrahydropterin synthase | insig. | 31 | 80 |
| | Acinetobacter baumannii LAC-4 | RR41_RS12105 | | 6-carboxytetrahydropterin synthase | strong | 29 | 83 |
| | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_2135 | | 6-pyruvoyl-tetrahydropterin synthase | strong | 28 | 81 |
| | Methylophilus sp. DMC18 | GFF1325 | | hypothetical protein | sig. | 34 | 57 |
| | Bacteroides ovatus ATCC 8483 | BACOVA_05260 | | hypothetical protein | insig. | 39 | 57 |
| | Methanococcus maripaludis JJ | MMJJ_RS07920 | | 6-carboxytetrahydropterin synthase QueD | cofit | 37 | 57 |
| | Methanococcus maripaludis S2 | MMP_RS06430 | | 6-carboxytetrahydropterin synthase QueD | sig. | 37 | 57 |
| | Mucilaginibacter yixingensis YX-36 DSM 26809 | ABZR88_RS16625 | | 6-carboxytetrahydropterin synthase | sig. | 28 | 90 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_02230 | | 6-pyruvoyltetrahydropterin/6-carboxytetrahydropterin synthase | strong | 32 | 57 |
| | Dechlorosoma suillum PS | Dsui_2137 | | queuosine biosynthesis protein QueD | insig. | 31 | 57 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS00210 | | 6-carboxytetrahydropterin synthase QueD | cofit | 31 | 57 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS12150 | | 6-carboxytetrahydropterin synthase QueD | sig. | 29 | 81 |
| | Paraburkholderia graminis OAS925 | ABIE53_000398 | | 6-pyruvoyltetrahydropterin/6- carboxytetrahydropterin synthase | strong | 32 | 57 |