| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Pseudomonas simiae WCS417 | PS417_05525 | | Fis family transcriptional regulator | strong | 100 | 100 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 98 | 100 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 98 | 100 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01292 | | Regulatory protein AtoC | cofit | 97 | 100 |
| o | Pseudomonas orientalis W4I3 | QF045_RS08450 | | sigma-54 dependent transcriptional regulator | insig. | 95 | 100 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_16225 | | Fis family transcriptional regulator | insig. | 88 | 100 |
| o | Xanthobacter sp. DMC5 | GFF4834 | | Regulatory protein AtoC | insig. | 51 | 100 |
| o | Escherichia coli BL21 | ECD_02147 | | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein | insig. | 44 | 98 |
| o | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01490 | | Regulatory protein AtoC | insig. | 44 | 98 |
| o | Escherichia coli BW25113 | b2220 | atoC | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein (NCBI) | insig. | 44 | 98 |
| o | Escherichia coli ECOR38 | HEPCGN_22430 | atoC | acetoacetate metabolism transcriptional regulator AtoC | insig. | 43 | 98 |
| o | Escherichia fergusonii Becca | EFB2_01635 | | Regulatory protein AtoC | insig. | 43 | 98 |
| o | Escherichia coli Nissle 1917 | ECOLIN_RS12965 | | acetoacetate metabolism transcriptional regulator AtoC | no data | 43 | 98 |
| o | Desulfovibrio vulgaris Hildenborough JW710 | DVU3220 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | no data | 46 | 98 |
| o | Methylophilus sp. DMC18 | GFF2815 | | Regulatory protein AtoC | insig. | 43 | 98 |
| o | Desulfovibrio vulgaris Miyazaki F | DvMF_1260 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | insig. | 44 | 98 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_0823 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | insig. | 43 | 97 |
| o | Klebsiella michiganensis M5al | BWI76_RS01605 | | transcriptional regulatory protein ZraR | insig. | 45 | 97 |
| o | Klebsiella pneumoniae MRSN742743 | KFA93_RS24385 | zraR;go_function=sequence-specific | sigma-54-dependent response regulator transcription factor ZraR | insig. | 45 | 97 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2200 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | no data | 44 | 98 |
| o | Klebsiella pneumoniae MKP103 | KDGMDA_10090 | | sigma-54-dependent response regulator transcription factor ZraR | insig. | 45 | 97 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS23090 | | sigma-54-dependent response regulator transcription factor ZraR | insig. | 45 | 98 |
| | Escherichia coli BL21 | ECD_03881 | | fused DNA-binding response regulator in two-component regulatory system with ZraS: response regulator/sigma54 interaction protein | insig. | 45 | 98 |
| | Escherichia coli BW25113 | b4004 | zraR | fused DNA-binding response regulator in two-component regulatory system with ZraS: response regulator/sigma54 interaction protein (NCBI) | insig. | 45 | 98 |
| | Escherichia fergusonii Becca | EFB2_04606 | | Transcriptional regulatory protein ZraR | insig. | 45 | 98 |
| o | Azospirillum sp. SherDot2 | MPMX19_03461 | | Regulatory protein AtoC | insig. | 44 | 98 |
| o | Escherichia coli ECRC100 | OKFHMN_13320 | zraR | sigma-54-dependent response regulator transcription factor ZraR | cofit | 45 | 98 |
| o | Escherichia coli ECRC62 | BNILDI_06055 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 45 | 98 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU1419 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | sig. | 44 | 98 |
| o | Escherichia coli ECRC101 | MCAODC_03820 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 45 | 98 |
| o | Escherichia coli ECRC102 | NIAGMN_11070 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 45 | 98 |
| o | Escherichia coli ECRC99 | KEDOAH_14840 | zraR | sigma-54-dependent response regulator transcription factor ZraR | no data | 45 | 98 |
| | Escherichia coli ECOR38 | HEPCGN_11960 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 45 | 98 |
| o | Escherichia coli ECOR27 | NOLOHH_05465 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 45 | 98 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_04045 | | Transcriptional regulatory protein ZraR | insig. | 45 | 98 |
| o | Escherichia coli ECRC98 | JDDGAC_16945 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 45 | 98 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU0118 | ntrC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | insig. | 43 | 98 |
| o | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF4188 | | Response regulator of zinc sigma-54-dependent two-component system | insig. | 44 | 97 |
| o | Rhodospirillum rubrum S1H | Rru_A1385 | | Two Component, Sigma54 Specific, Transcriptional Regulator, Fis family (NCBI) | cofit | 43 | 98 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5122 | | Type IV fimbriae expression regulatory protein PilR | insig. | 42 | 98 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF610 | | Putative sensory histidine kinase YfhA | insig. | 44 | 97 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_2818 | | Type IV fimbriae expression regulatory protein PilR | insig. | 43 | 98 |
| o | Pseudomonas sp. DMC3 | GFF4000 | | Regulatory protein AtoC | cofit | 42 | 98 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_15905 | | transcriptional regulator | insig. | 42 | 98 |
| o | Pseudomonas sp. RS175 | PFR28_03751 | | Regulatory protein AtoC | insig. | 42 | 98 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1461 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | no data | 43 | 99 |
| o | Cellulophaga baltica 18 | M666_RS02825 | | sigma-54 dependent transcriptional regulator | strong | 42 | 98 |
| o | Pseudomonas segetis P6 | ACVTMO_RS19460 | | sigma-54-dependent transcriptional regulator | cofit | 43 | 97 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2109 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 42 | 98 |
| o | Pseudomonas syringae pv. syringae B728a | Psyr_0722 | | Two-component response regulator PilR | insig. | 43 | 98 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_0722 | | Two-component response regulator PilR | insig. | 43 | 98 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_0215 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 47 | 84 |
| o | Variovorax sp. OAS795 | ABID97_RS06460 | | sigma 54-interacting transcriptional regulator | cofit | 44 | 97 |
| o | Pseudomonas sp. S08-1 | OH686_16390 | | Type IV fimbriae expression regulatory protein PilR | cofit | 42 | 97 |
| o | Pseudomonas aeruginosa MRSN321 | DY961_RS23710 | pilR;inference=COORDINATES | two-component system response regulator PilR | strong | 41 | 97 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_00790 | | two-component response regulator PilR | strong | 41 | 97 |
| o | Pseudomonas aeruginosa PUPa3 | DQ20_RS56175 | pilR;inference=COORDINATES | two-component system response regulator PilR | strong | 41 | 97 |
| o | Dechlorosoma suillum PS | Dsui_2360 | | response regulator with CheY-like receiver, AAA-type ATPase, and DNA-binding domains | cofit | 45 | 97 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_14200 | | transcriptional regulator | insig. | 41 | 98 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2934 | ntrX | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | no data | 43 | 93 |
| o | Variovorax sp. SCN45 | GFF7323 | | Transcriptional response regulatory protein GlrR | insig. | 43 | 97 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_1489 | | Type IV fimbriae expression regulatory protein PilR | insig. | 41 | 98 |
| o | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_16520 | | DNA-binding response regulator | insig. | 39 | 98 |
| o | Herbaspirillum seropedicae SmR1 | HSERO_RS23170 | | ATPase AAA | insig. | 44 | 98 |
| o | Caulobacter crescentus NA1000 | CCNA_03424 | | AAA-family response regulator tacA | strong | 39 | 99 |
| o | Caulobacter crescentus NA1000 Δfur | CCNA_03424 | | AAA-family response regulator tacA | cofit | 39 | 99 |
| o | Pseudomonas stutzeri RCH2 | Psest_0705 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | strong | 41 | 97 |
| | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_09510 | | sigma-54-dependent Fis family transcriptional regulator | sig. | 39 | 98 |
| o | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS05020 | | sigma-54 dependent transcriptional regulator | strong | 41 | 98 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2149 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 41 | 98 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU0110 | ntrC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | cofit | 41 | 99 |
| | Escherichia fergusonii Becca | EFB2_04748 | | DNA-binding transcriptional regulator NtrC | strong | 42 | 98 |
| o | Dickeya dadantii 3937 | DDA3937_RS15640 | | two-component system response regulator GlrR | cofit | 42 | 97 |
| | Escherichia coli ECOR27 | NOLOHH_06240 | glnG | nitrogen regulation protein NR(I) | insig. | 42 | 98 |
| | Escherichia coli ECRC100 | OKFHMN_14110 | glnG | nitrogen regulation protein NR(I) | insig. | 42 | 98 |
| | Escherichia coli ECRC101 | MCAODC_04610 | glnG | nitrogen regulation protein NR(I) | strong | 42 | 98 |
| | Escherichia coli ECRC102 | NIAGMN_11865 | glnG | nitrogen regulation protein NR(I) | insig. | 42 | 98 |
| | Escherichia coli ECRC98 | JDDGAC_17735 | glnG | nitrogen regulation protein NR(I) | no data | 42 | 98 |
| | Escherichia coli ECRC99 | KEDOAH_14040 | glnG | nitrogen regulation protein NR(I) | no data | 42 | 98 |
| | Escherichia coli BW25113 | b3868 | glnG | fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) | strong | 42 | 98 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS22295 | | nitrogen regulation protein NR(I) | strong | 42 | 98 |
| | Escherichia coli BL21 | ECD_03753 | | fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein | insig. | 41 | 98 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_04184 | | DNA-binding transcriptional regulator NtrC | sig. | 41 | 98 |
| o | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS09115 | | sigma-54 dependent transcriptional regulator | insig. | 45 | 85 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF2220 | | Nitrogen regulation protein NR(I) | strong | 42 | 98 |
| | Escherichia coli ECRC62 | BNILDI_05290 | glnG | nitrogen regulation protein NR(I) | cofit | 41 | 98 |
| o | Pantoea sp. MT58 | IAI47_04945 | | two-component system response regulator GlrR | sig. | 41 | 97 |
| o | Agrobacterium fabrum C58 | Atu3764 | | sigma 54 dependent transcriptional activator | sig. | 40 | 100 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_28370 | | two-component system response regulator QseF/GlrR | insig. | 42 | 97 |
| | Klebsiella pneumoniae MRSN742743 | KFA93_RS04790 | glrR;go_function=ATP | two-component system response regulator GlrR | sig. | 42 | 97 |
| | Escherichia coli ECOR38 | HEPCGN_12740 | glnG | nitrogen regulation protein NR(I) | insig. | 42 | 98 |
| o | Dickeya dianthicola 67-19 | HGI48_RS15735 | | two-component system response regulator GlrR | insig. | 42 | 97 |
| o | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11070 | | sigma-54 dependent transcriptional regulator | cofit | 40 | 98 |
| | Agrobacterium fabrum C58 | Atu1446 | | two component response regulator | strong | 41 | 100 |
| o | Dickeya dianthicola ME23 | DZA65_RS16815 | | two-component system response regulator GlrR | insig. | 42 | 97 |
| | Enterobacter sp. TBS_079 | MPMX20_04597 | | DNA-binding transcriptional regulator NtrC | strong | 41 | 98 |
| | Serratia liquefaciens MT49 | IAI46_24960 | | nitrogen regulation protein NR(I) | strong | 41 | 98 |
| o | Parabacteroides merdae CL09T00C40 | HMPREF1078_RS14460 | | sigma-54 dependent transcriptional regulator | insig. | 40 | 98 |
| o | Pectobacterium carotovorum WPP14 | HER17_RS05925 | | two-component system response regulator GlrR | cofit | 41 | 97 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_4350 | | nitrogen regulation protein NR(I) | insig. | 41 | 98 |