| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Dechlorosoma suillum PS | Dsui_0135 | | response regulator with CheY-like receiver, AAA-type ATPase, and DNA-binding domains | cofit | 100 | 100 |
| o | Methylophilus sp. DMC18 | GFF2304 | | Regulatory protein AtoC | cofit | 58 | 96 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5122 | | Type IV fimbriae expression regulatory protein PilR | insig. | 61 | 97 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_00790 | | two-component response regulator PilR | strong | 61 | 97 |
| o | Pseudomonas sp. RS175 | PFR28_03751 | | Regulatory protein AtoC | insig. | 60 | 97 |
| o | Pseudomonas syringae pv. syringae B728a | Psyr_0722 | | Two-component response regulator PilR | insig. | 59 | 97 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_0722 | | Two-component response regulator PilR | insig. | 59 | 97 |
| o | Pseudomonas stutzeri RCH2 | Psest_0705 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | strong | 59 | 98 |
| o | Pseudomonas segetis P6 | MPMX49_03926 | | Regulatory protein AtoC | cofit | 58 | 97 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_14200 | | transcriptional regulator | insig. | 60 | 97 |
| o | Pseudomonas sp. S08-1 | OH686_16390 | | Type IV fimbriae expression regulatory protein PilR | cofit | 60 | 97 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_15905 | | transcriptional regulator | insig. | 59 | 97 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_2818 | | Type IV fimbriae expression regulatory protein PilR | insig. | 59 | 97 |
| o | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS05020 | | sigma-54 dependent transcriptional regulator | strong | 58 | 96 |
| o | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11070 | | sigma-54 dependent transcriptional regulator | cofit | 57 | 96 |
| o | Pseudomonas sp. DMC3 | GFF4000 | | Regulatory protein AtoC | cofit | 59 | 97 |
| o | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS04990 | | sigma-54 dependent transcriptional regulator | cofit | 58 | 96 |
| o | Rhodanobacter denitrificans MT42 | LRK55_RS04800 | | sigma-54 dependent transcriptional regulator | cofit | 57 | 96 |
| o | Rhodanobacter sp. FW510-T8 | OKGIIK_01730 | atoC | sigma-54-dependent Fis family transcriptional regulator | insig. | 57 | 96 |
| o | Lysobacter sp. OAE881 | ABIE51_RS04850 | | sigma-54 dependent transcriptional regulator | strong | 55 | 96 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_1489 | | Type IV fimbriae expression regulatory protein PilR | insig. | 56 | 97 |
| o | Marinobacter adhaerens HP15 | HP15_523 | | two component, sigma54 specific, transcriptional regulator, Fis family | strong | 54 | 96 |
| o | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.1353.1 | | Type IV fimbriae expression regulatory protein PilR | sig. | 57 | 94 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS01750 | | sigma-54 dependent transcriptional regulator | strong | 53 | 96 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS17870 | | Fis family transcriptional regulator | insig. | 51 | 96 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS11155 | | sigma-54-dependent Fis family transcriptional regulator | cofit | 63 | 81 |
| | Ralstonia solanacearum GMI1000 | RS_RS14035 | | sigma-54-dependent Fis family transcriptional regulator | strong | 63 | 81 |
| | Ralstonia solanacearum UW163 | UW163_RS07540 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 62 | 81 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS06085 | | sigma-54-dependent Fis family transcriptional regulator | strong | 62 | 81 |
| o | Acinetobacter baumannii LAC-4 | RR41_RS17800 | | sigma-54 dependent transcriptional regulator | insig. | 46 | 96 |
| o | Acidovorax sp. GW101-3H11 | Ac3H11_4019 | | Sigma-54 dependent transcriptional regulator | sig. | 48 | 97 |
| o | Acinetobacter radioresistens SK82 | MPMX26_00162 | | Regulatory protein AtoC | cofit | 44 | 97 |
| o | Variovorax sp. SCN45 | GFF4432 | | Two-component system response regulator protein | strong | 47 | 97 |
| o | Variovorax sp. OAS795 | ABID97_RS06415 | | sigma-54 dependent transcriptional regulator | strong | 48 | 97 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF595 | | two component, sigma54 specific, transcriptional regulator, Fis family | strong | 50 | 97 |
| o | Xanthobacter sp. DMC5 | GFF4834 | | Regulatory protein AtoC | insig. | 45 | 97 |
| | Escherichia fergusonii Becca | EFB2_01635 | | Regulatory protein AtoC | insig. | 41 | 97 |
| | Escherichia coli ECOR38 | HEPCGN_22430 | atoC | acetoacetate metabolism transcriptional regulator AtoC | insig. | 41 | 97 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS12965 | | acetoacetate metabolism transcriptional regulator AtoC | no data | 41 | 97 |
| | Escherichia coli BL21 | ECD_02147 | | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein | insig. | 40 | 97 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01490 | | Regulatory protein AtoC | insig. | 40 | 97 |
| | Escherichia coli BW25113 | b2220 | atoC | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein (NCBI) | insig. | 40 | 97 |
| o | Desulfovibrio vulgaris Hildenborough JW710 | DVU3305 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | strong | 46 | 97 |
| o | Desulfovibrio vulgaris Miyazaki F | DvMF_2109 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 43 | 97 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_0823 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | insig. | 42 | 97 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_10090 | | sigma-54-dependent response regulator transcription factor ZraR | insig. | 43 | 96 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_16225 | | Fis family transcriptional regulator | insig. | 43 | 96 |
| | Klebsiella michiganensis M5al | BWI76_RS01605 | | transcriptional regulatory protein ZraR | insig. | 44 | 96 |
| | Escherichia coli ECOR27 | NOLOHH_05465 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 44 | 96 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_04045 | | Transcriptional regulatory protein ZraR | insig. | 44 | 96 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS23090 | | sigma-54-dependent response regulator transcription factor ZraR | insig. | 44 | 96 |
| | Dickeya dadantii 3937 | DDA3937_RS00125 | | nitrogen regulation protein NR(I) | cofit | 40 | 96 |
| | Methylophilus sp. DMC18 | GFF2024 | | DNA-binding transcriptional regulator NtrC | strong | 39 | 96 |
| | Escherichia coli BL21 | ECD_03881 | | fused DNA-binding response regulator in two-component regulatory system with ZraS: response regulator/sigma54 interaction protein | insig. | 44 | 96 |
| | Escherichia coli BW25113 | b4004 | zraR | fused DNA-binding response regulator in two-component regulatory system with ZraS: response regulator/sigma54 interaction protein (NCBI) | insig. | 44 | 96 |
| | Escherichia coli ECRC101 | MCAODC_03820 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 44 | 96 |
| | Escherichia coli ECRC102 | NIAGMN_11070 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 44 | 96 |
| | Escherichia coli ECRC99 | KEDOAH_14840 | zraR | sigma-54-dependent response regulator transcription factor ZraR | no data | 44 | 96 |
| | Escherichia fergusonii Becca | EFB2_04606 | | Transcriptional regulatory protein ZraR | insig. | 44 | 96 |
| | Escherichia coli ECRC62 | BNILDI_06055 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 44 | 96 |
| | Escherichia coli ECOR38 | HEPCGN_11960 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 44 | 96 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_04184 | | DNA-binding transcriptional regulator NtrC | insig. | 40 | 96 |
| | Dickeya dianthicola 67-19 | HGI48_RS21235 | | nitrogen regulation protein NR(I) | strong | 40 | 96 |
| | Dickeya dianthicola ME23 | DZA65_RS22210 | | nitrogen regulation protein NR(I) | strong | 40 | 96 |
| | Pectobacterium carotovorum WPP14 | HER17_RS21440 | | nitrogen regulation protein NR(I) | cofit | 39 | 96 |
| | Escherichia coli ECRC98 | JDDGAC_16945 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 44 | 96 |
| | Escherichia coli ECRC100 | OKFHMN_13320 | zraR | sigma-54-dependent response regulator transcription factor ZraR | cofit | 44 | 96 |
| | Escherichia fergusonii Becca | EFB2_04748 | | DNA-binding transcriptional regulator NtrC | strong | 40 | 96 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS22295 | | nitrogen regulation protein NR(I) | strong | 40 | 96 |
| | Pseudomonas simiae WCS417 | PS417_05525 | | Fis family transcriptional regulator | strong | 42 | 97 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01292 | | Regulatory protein AtoC | cofit | 42 | 97 |
| o | Kangiella aquimarina DSM 16071 | B158DRAFT_2527 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | sig. | 39 | 97 |
| | Escherichia coli ECOR27 | NOLOHH_06240 | glnG | nitrogen regulation protein NR(I) | insig. | 40 | 96 |
| | Escherichia coli ECOR38 | HEPCGN_12740 | glnG | nitrogen regulation protein NR(I) | insig. | 40 | 96 |
| | Escherichia coli ECRC100 | OKFHMN_14110 | glnG | nitrogen regulation protein NR(I) | insig. | 40 | 96 |
| | Escherichia coli ECRC101 | MCAODC_04610 | glnG | nitrogen regulation protein NR(I) | strong | 40 | 96 |
| | Escherichia coli ECRC102 | NIAGMN_11865 | glnG | nitrogen regulation protein NR(I) | insig. | 40 | 96 |
| | Escherichia coli ECRC98 | JDDGAC_17735 | glnG | nitrogen regulation protein NR(I) | insig. | 40 | 96 |
| | Escherichia coli ECRC99 | KEDOAH_14040 | glnG | nitrogen regulation protein NR(I) | no data | 40 | 96 |
| | Escherichia coli BW25113 | b3868 | glnG | fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) | strong | 40 | 96 |
| | Escherichia coli ECRC62 | BNILDI_05290 | glnG | nitrogen regulation protein NR(I) | cofit | 40 | 96 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 42 | 96 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 42 | 96 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_09000 | | nitrogen regulation protein NR(I) | insig. | 39 | 96 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF4188 | | Response regulator of zinc sigma-54-dependent two-component system | insig. | 43 | 96 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_0215 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 45 | 85 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF2220 | | Nitrogen regulation protein NR(I) | strong | 40 | 96 |
| | Erwinia amylovora T8 | OLJFJH_05495 | | nitrogen regulation protein NR(I) | strong | 39 | 96 |
| | Pantoea sp. MT58 | IAI47_00450 | | nitrogen regulation protein NR(I) | strong | 39 | 96 |
| o | Cellulophaga baltica 18 | M666_RS02825 | | sigma-54 dependent transcriptional regulator | cofit | 38 | 95 |
| | Escherichia coli BL21 | ECD_03753 | | fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein | insig. | 40 | 96 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_4350 | | nitrogen regulation protein NR(I) | insig. | 39 | 96 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS30045 | | ATPase AAA | insig. | 43 | 97 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_16075 | | Fis family transcriptional regulator | strong | 41 | 97 |
| | Enterobacter sp. TBS_079 | MPMX20_04597 | | DNA-binding transcriptional regulator NtrC | strong | 39 | 96 |
| | Serratia liquefaciens MT49 | IAI46_24960 | | nitrogen regulation protein NR(I) | strong | 39 | 96 |
| | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS18895 | | nitrogen regulation protein NR(I) | strong | 39 | 96 |
| | Klebsiella michiganensis M5al | BWI76_RS00385 | | nitrogen assimilation regulatory protein | strong | 39 | 96 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1993 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | sig. | 39 | 97 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_14400 | | Fis family transcriptional regulator | strong | 41 | 97 |