| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_10670 | | sigma-54-dependent Fis family transcriptional regulator | cofit | 100 | 100 |
| o | Shewanella oneidensis MR-1 | SO3232 | flrA | flagellar regulatory protein A (NCBI ptt file) | strong | 58 | 100 |
| o | Shewanella sp. ANA-3 | Shewana3_1338 | | sigma-54 dependent trancsriptional regulator (RefSeq) | strong | 58 | 100 |
| o | Shewanella amazonensis SB2B | Sama_2301 | | response regulator receiver protein (RefSeq) | strong | 58 | 100 |
| o | Shewanella loihica PV-4 | Shew_1362 | | sigma-54 dependent trancsriptional regulator (RefSeq) | strong | 56 | 100 |
| o | Alteromonas macleodii MIT1002 | MIT1002_01081 | | Nitrogen assimilation regulatory protein | cofit | 54 | 99 |
| o | Pseudomonas stutzeri RCH2 | Psest_1765 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | strong | 54 | 99 |
| o | Pseudomonas orientalis W4I3 | QF045_RS21955 | | sigma-54 dependent transcriptional regulator | cofit | 53 | 99 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_03855 | | AAA family ATPase | strong | 52 | 99 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_2597 | | Flagellar regulatory protein FleQ | strong | 52 | 99 |
| o | Pseudomonas sp. RS175 | PFR28_00828 | | Anaerobic nitric oxide reductase transcription regulator NorR | strong | 52 | 99 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS21795 | | sigma-54-dependent Fis family transcriptional regulator | strong | 53 | 99 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS21795 | | sigma-54-dependent Fis family transcriptional regulator | strong | 53 | 99 |
| o | Pseudomonas syringae pv. syringae B728a | Psyr_3461 | | Helix-turn-helix, Fis-type | strong | 52 | 99 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3461 | | Helix-turn-helix, Fis-type | cofit | 52 | 99 |
| o | Pseudomonas simiae WCS417 | PS417_19825 | | ATPase AAA | strong | 52 | 99 |
| o | Pseudomonas sp. SVBP6 | COO64_RS21300 | fleQ | transcriptional regulator FleQ | cofit | 52 | 99 |
| o | Pseudomonas sp. BP01 | JOY50_RS00705 | fleQ | transcriptional regulator FleQ | sig. | 53 | 99 |
| o | Pseudomonas sp. DMC3 | GFF2445 | | Anaerobic nitric oxide reductase transcription regulator NorR | cofit | 52 | 99 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_3599 | | Flagellar regulatory protein FleQ | insig. | 52 | 99 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_18660 | | AAA family ATPase | strong | 52 | 99 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_04163 | | Anaerobic nitric oxide reductase transcription regulator NorR | strong | 52 | 99 |
| o | Pseudomonas putida KT2440 | PP_4373 | | Transcriptional regulator FleQ | strong | 52 | 99 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_4357 | | Flagellar regulatory protein FleQ | insig. | 52 | 99 |
| o | Pseudomonas sp. S08-1 | OH686_02855 | | Flagellar regulatory protein FleQ | cofit | 52 | 100 |
| o | Marinobacter adhaerens HP15 | HP15_2330 | | two component, sigma54 specific, transcriptional regulator, Fis family | strong | 51 | 99 |
| o | Pseudomonas segetis P6 | MPMX49_03150 | | Regulatory protein AtoC | strong | 52 | 100 |
| o | Pseudomonas aeruginosa MRSN321 | DY961_RS06965 | fleQ;inference=COORDINATES | transcriptional regulator FleQ | strong | 53 | 100 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_05045 | | transcriptional regulator FleQ | strong | 53 | 100 |
| o | Pseudomonas aeruginosa PUPa3 | DQ20_RS45870 | fleQ;inference=COORDINATES | transcriptional regulator FleQ | strong | 53 | 100 |
| o | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS15090 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 47 | 99 |
| o | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS13485 | | sigma-54-dependent Fis family transcriptional regulator | strong | 48 | 100 |
| o | Rhodanobacter denitrificans MT42 | LRK55_RS13225 | | sigma-54-dependent Fis family transcriptional regulator | cofit | 48 | 100 |
| o | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS20155 | | sigma-54 dependent transcriptional regulator | insig. | 50 | 99 |
| o | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.2800.1 | | Flagellar regulatory protein FleQ | sig. | 50 | 90 |
| o | Lysobacter sp. OAE881 | ABIE51_RS16770 | | sigma-54 dependent transcriptional regulator | cofit | 45 | 100 |
| o | Rhodanobacter sp. FW510-T8 | OKGIIK_02735 | | Fis family transcriptional regulator | cofit | 47 | 99 |
| | Sinorhizobium meliloti 1021 | SMc01043 | | nitrogen assimilation regulatory protein | strong | 40 | 98 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1324 | | sigma54 specific transcriptional regulator, Fis family (RefSeq) | strong | 47 | 71 |
| | Rhizobium sp. OAE497 | ABIE40_RS09090 | | nitrogen regulation protein NR(I) | strong | 39 | 98 |
| | Rhodospirillum rubrum S1H | Rru_A1388 | | Transcriptional Regulator, NifA subfamily, Fis Family (NCBI) | cofit | 45 | 72 |
| | Agrobacterium fabrum C58 | Atu1446 | | two component response regulator | strong | 38 | 98 |
| | Rhodopseudomonas palustris CGA009 | TX73_007055 | | sigma-54-dependent Fis family transcriptional regulator | sig. | 44 | 72 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2934 | ntrX | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | no data | 44 | 72 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS11950 | | nitrogen regulation protein NR(I) | no data | 45 | 72 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1461 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | no data | 38 | 99 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU3220 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | no data | 42 | 72 |
| | Rhodospirillum rubrum S1H | Rru_A1678 | | Nitrogen Metabolism Transcriptional Regulator, NtrC, Fis Family (NCBI) | strong | 37 | 99 |
| | Escherichia coli BW25113 | b2491 | hyfR | putative 2-component regulator, interaction with sigma 54 (VIMSS) | sig. | 40 | 86 |
| | Escherichia coli ECRC100 | OKFHMN_21850 | hyfR | DNA-binding transcriptional regulator HyfR | insig. | 39 | 89 |
| | Escherichia coli ECRC101 | MCAODC_12380 | hyfR | DNA-binding transcriptional regulator HyfR | insig. | 39 | 89 |
| | Escherichia coli ECRC102 | NIAGMN_19620 | hyfR | DNA-binding transcriptional regulator HyfR | insig. | 39 | 89 |
| | Escherichia coli ECRC98 | JDDGAC_25795 | hyfR | DNA-binding transcriptional regulator HyfR | insig. | 39 | 89 |
| | Escherichia coli ECRC99 | KEDOAH_06370 | hyfR | DNA-binding transcriptional regulator HyfR | no data | 39 | 89 |
| | Escherichia coli BL21 | ECD_02383 | | hydrogenase-4 transcriptional activator | cofit | 40 | 86 |
| | Escherichia coli ECRC62 | BNILDI_12310 | hyfR | DNA-binding transcriptional regulator HyfR | insig. | 40 | 86 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01235 | | DNA-binding transcriptional activator HyfR | cofit | 40 | 86 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2894 | flrA | sigma-54 dependent transcriptional regulator (TIGR) | strong | 44 | 71 |
| | Rhodopseudomonas palustris CGA009 | TX73_013420 | | nitrogen regulation protein NR(I) | strong | 44 | 72 |
| | Escherichia coli ECOR38 | HEPCGN_21015 | hyfR | DNA-binding transcriptional activator HyfR | insig. | 43 | 76 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2200 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | no data | 42 | 72 |
| | Xanthobacter sp. DMC5 | GFF848 | | DNA-binding transcriptional regulator NtrC | no data | 36 | 99 |
| | Azospirillum brasilense Sp245 | AZOBR_RS14430 | | chemotaxis protein CheY | strong | 44 | 72 |
| | Azospirillum brasilense Sp245 | AZOBR_RS29300 | | Fis family transcriptional regulator | insig. | 43 | 72 |
| | Cellulophaga baltica 18 | M666_RS12540 | | sigma 54-interacting response regulator | insig. | 47 | 56 |
| | Bosea sp. OAE506 | ABIE41_RS16785 | | nitrogen regulation protein NR(I) | insig. | 46 | 72 |
| | Escherichia coli ECOR27 | NOLOHH_13500 | | Fis family transcriptional regulator | insig. | 46 | 69 |
| | Azospirillum sp. SherDot2 | MPMX19_01112 | | DNA-binding transcriptional regulator NtrC | no data | 44 | 72 |
| | Rahnella sp. WP5 | EX31_RS15395 | | nif-specific transcriptional activator NifA | insig. | 54 | 49 |
| | Dickeya dadantii 3937 | DDA3937_RS18805 | | nif-specific transcriptional activator NifA | insig. | 44 | 73 |
| | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_04380 | | response regulator | strong | 41 | 74 |
| | Dickeya dianthicola ME23 | DZA65_RS19920 | | nif-specific transcriptional activator NifA | insig. | 44 | 73 |
| | Rhodopseudomonas palustris CGA009 | TX73_007390 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 43 | 72 |
| | Dechlorosoma suillum PS | Dsui_0940 | | Nif-specific regulatory protein | strong | 55 | 49 |
| | Dechlorosoma suillum PS | Dsui_1051 | | nitrogen regulation protein NR(I) | strong | 44 | 72 |
| | Rhodopseudomonas palustris CGA009 | TX73_024055 | | nif-specific transcriptional activator NifA | strong | 42 | 74 |
| | Dickeya dianthicola 67-19 | HGI48_RS18785 | | nif-specific transcriptional activator NifA | insig. | 44 | 73 |
| | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_0196 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | strong | 41 | 74 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 38 | 85 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 38 | 85 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_16225 | | Fis family transcriptional regulator | insig. | 42 | 72 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1933 | | PAS modulated sigma54 specific transcriptional regulator, Fis family (RefSeq) | insig. | 39 | 80 |
| | Rhodospirillum rubrum S1H | Rru_A1385 | | Two Component, Sigma54 Specific, Transcriptional Regulator, Fis family (NCBI) | cofit | 44 | 70 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_0823 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | insig. | 39 | 85 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_03040 | | formate hydrogenlyase transcriptional activator FlhA | insig. | 42 | 73 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2149 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 36 | 99 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01292 | | Regulatory protein AtoC | cofit | 43 | 73 |
| | Rhodospirillum rubrum S1H | Rru_A0326 | | Transcriptional Regulator, NifA subfamily, Fis Family (NCBI) | insig. | 41 | 78 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2114 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | insig. | 35 | 99 |
| | Escherichia fergusonii Becca | EFB2_01635 | | Regulatory protein AtoC | insig. | 43 | 68 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS12965 | | acetoacetate metabolism transcriptional regulator AtoC | no data | 43 | 68 |
| | Escherichia coli ECOR38 | HEPCGN_22430 | atoC | acetoacetate metabolism transcriptional regulator AtoC | insig. | 43 | 68 |
| | Escherichia coli BL21 | ECD_02147 | | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein | insig. | 43 | 68 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01490 | | Regulatory protein AtoC | insig. | 43 | 68 |
| | Escherichia coli BW25113 | b2220 | atoC | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein (NCBI) | insig. | 43 | 68 |
| | Klebsiella michiganensis M5al | BWI76_RS22370 | | transcriptional regulator FhlA | insig. | 42 | 73 |
| | Escherichia coli ECOR38 | HEPCGN_11960 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 41 | 80 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS23090 | | sigma-54-dependent response regulator transcription factor ZraR | insig. | 41 | 80 |
| | Escherichia coli ECOR27 | NOLOHH_05465 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 40 | 80 |
| | Alteromonas macleodii MIT1002 | MIT1002_03048 | | Transcriptional regulatory protein ZraR | insig. | 40 | 74 |