| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Variovorax sp. SCN45 | GFF2200 | | sigma-54-dependent transcriptional regulator | insig. | 100 | 100 |
| o | Variovorax sp. OAS795 | ABID97_RS24330 | | sigma 54-interacting transcriptional regulator | insig. | 92 | 100 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS08215 | | Fis family transcriptional regulator | insig. | 64 | 100 |
| o | Paraburkholderia graminis OAS925 | ABIE53_002431 | | DNA-binding NtrC family response regulator | insig. | 64 | 100 |
| o | Ralstonia solanacearum PSI07 | RPSI07_RS05015 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 56 | 98 |
| o | Ralstonia solanacearum GMI1000 | RS_RS22325 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 56 | 98 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS19575 | | sigma-54-dependent Fis family transcriptional regulator | sig. | 55 | 98 |
| o | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS18540 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 55 | 98 |
| o | Ralstonia solanacearum UW163 | UW163_RS20590 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 55 | 98 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS35955 | | Fis family transcriptional regulator | insig. | 54 | 97 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS05020 | | sigma-54 dependent transcriptional regulator | strong | 41 | 69 |
| | Marinobacter adhaerens HP15 | HP15_523 | | two component, sigma54 specific, transcriptional regulator, Fis family | strong | 41 | 69 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11070 | | sigma-54 dependent transcriptional regulator | cofit | 42 | 70 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS04990 | | sigma-54 dependent transcriptional regulator | cofit | 41 | 69 |
| | Rhodopseudomonas palustris CGA009 | TX73_007390 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 36 | 69 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS04800 | | sigma-54 dependent transcriptional regulator | cofit | 39 | 69 |
| | Rhodanobacter sp. FW510-T8 | OKGIIK_01730 | atoC | sigma-54-dependent Fis family transcriptional regulator | cofit | 40 | 69 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS23520 | | sigma-54 dependent transcriptional regulator | insig. | 39 | 77 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS42175 | | nif-specific transcriptional activator NifA | insig. | 37 | 73 |
| | Paraburkholderia graminis OAS925 | ABIE53_005506 | | transcriptional regulator of acetoin/glycerol metabolism | strong | 40 | 64 |
| | Alteromonas macleodii MIT1002 | MIT1002_01081 | | Nitrogen assimilation regulatory protein | cofit | 37 | 69 |
| | Pseudomonas sp. SVBP6 | COO64_RS13325 | ntrC | nitrogen regulation protein NR(I) | insig. | 36 | 86 |
| | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_09510 | | sigma-54-dependent Fis family transcriptional regulator | sig. | 36 | 74 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1213 | | sigma54 specific transcriptional regulator, Fis family (RefSeq) | insig. | 36 | 73 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_347 | | Nitrogen regulation protein NR(I) | strong | 36 | 86 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_00790 | | two-component response regulator PilR | strong | 35 | 86 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS56175 | pilR;inference=COORDINATES | two-component system response regulator PilR | strong | 35 | 86 |
| | Methylophilus sp. DMC18 | GFF1426 | | Transcriptional regulatory protein ZraR | insig. | 39 | 68 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS23310 | | sigma-54-dependent Fis family transcriptional regulator | strong | 39 | 70 |
| | Ralstonia solanacearum UW163 | UW163_RS16560 | | sigma-54-dependent Fis family transcriptional regulator | strong | 39 | 70 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS23710 | pilR;inference=COORDINATES | two-component system response regulator PilR | strong | 35 | 86 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_12145 | | nitrogen regulation protein NR(I) | strong | 36 | 86 |
| | Rahnella sp. WP5 | EX31_RS15395 | | nif-specific transcriptional activator NifA | insig. | 39 | 69 |
| | Ralstonia solanacearum GMI1000 | RS_RS25110 | | sigma-54-dependent Fis family transcriptional regulator | cofit | 39 | 69 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2149 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 38 | 78 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_4822 | | Helix-turn-helix, Fis-type:Nitrogen regulation protein NR(I) | sig. | 36 | 86 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_4822 | | Helix-turn-helix, Fis-type:Nitrogen regulation protein NR(I) | no data | 36 | 86 |
| | Pseudomonas sp. DMC3 | GFF4081 | | DNA-binding transcriptional regulator NtrC | strong | 35 | 86 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2934 | ntrX | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | no data | 37 | 72 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 36 | 81 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 36 | 81 |
| | Azospirillum sp. SherDot2 | MPMX19_03461 | | Regulatory protein AtoC | insig. | 40 | 69 |
| | Variovorax sp. OAS795 | ABID97_RS06460 | | sigma 54-interacting transcriptional regulator | cofit | 39 | 69 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_3826 | | Nitrogen regulation protein NR(I) | strong | 35 | 86 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_13865 | | nitrogen regulation protein NR(I) | insig. | 33 | 86 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_09730 | | nitrogen regulation protein NR(I) | strong | 35 | 86 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU0118 | ntrC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | insig. | 40 | 69 |
| | Pseudomonas stutzeri RCH2 | Psest_0705 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | strong | 38 | 75 |
| | Azospirillum brasilense Sp245 | AZOBR_RS29300 | | Fis family transcriptional regulator | insig. | 36 | 70 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01292 | | Regulatory protein AtoC | cofit | 36 | 81 |
| | Pseudomonas sp. RS175 | PFR28_04944 | | DNA-binding transcriptional regulator NtrC | strong | 35 | 86 |
| | Pseudomonas simiae WCS417 | PS417_15295 | | Fis family transcriptional regulator | insig. | 39 | 71 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS03980 | | Fis family transcriptional regulator | strong | 36 | 86 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_1611 | | Nitrogen regulation protein NR(I) | strong | 35 | 86 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS19345 | | sigma-54 dependent transcriptional regulator | no data | 38 | 71 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_16225 | | Fis family transcriptional regulator | insig. | 37 | 72 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS01685 | | nitrogen regulation protein NR(I) | sig. | 35 | 86 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS01685 | | nitrogen regulation protein NR(I) | strong | 35 | 86 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_00547 | | DNA-binding transcriptional regulator NtrC | insig. | 35 | 86 |
| | Pseudomonas simiae WCS417 | PS417_01635 | | nitrogen regulation protein NR(I) | strong | 35 | 86 |
| | Marinobacter adhaerens HP15 | HP15_425 | | two-component response regulator NtrC | strong | 38 | 68 |
| | Pseudomonas sp. BP01 | JOY50_RS09450 | ntrC | nitrogen regulation protein NR(I) | cofit | 35 | 86 |
| | Shewanella loihica PV-4 | Shew_3561 | | nitrogen metabolism transcriptional regulator, NtrC, Fis family (RefSeq) | strong | 34 | 81 |
| | Shewanella amazonensis SB2B | Sama_2301 | | response regulator receiver protein (RefSeq) | strong | 38 | 68 |
| | Pseudomonas sp. S08-1 | OH686_02855 | | Flagellar regulatory protein FleQ | cofit | 38 | 78 |
| | Pseudomonas sp. S08-1 | OH686_13325 | | Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake | cofit | 36 | 81 |
| | Pseudomonas orientalis W4I3 | QF045_RS04705 | | nitrogen regulation protein NR(I) | insig. | 36 | 86 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF610 | | Putative sensory histidine kinase YfhA | insig. | 46 | 53 |
| | Variovorax sp. SCN45 | GFF7323 | | Transcriptional response regulatory protein GlrR | insig. | 38 | 69 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS11455 | ntrC;inference=COORDINATES | two-component system response regulator NtrC | strong | 36 | 86 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_27895 | | two-component system response regulator NtrC | insig. | 36 | 86 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS50645 | ntrC;inference=COORDINATES | two-component system response regulator NtrC | strong | 36 | 86 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_18205 | | Fis family transcriptional regulator | insig. | 37 | 73 |
| | Pseudomonas sp. S08-1 | OH686_10640 | | nitrogen regulation protein NR(I) | cofit | 35 | 86 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_14720 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 33 | 93 |
| | Pseudomonas sp. SVBP6 | COO64_RS07975 | | sigma-54 dependent transcriptional regulator | insig. | 39 | 68 |
| | Parabacteroides merdae CL09T00C40 | HMPREF1078_RS14460 | | sigma-54 dependent transcriptional regulator | insig. | 38 | 69 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS12980 | | chemotaxis protein CheY | strong | 40 | 67 |
| | Dickeya dianthicola ME23 | DZA65_RS19920 | | nif-specific transcriptional activator NifA | insig. | 39 | 70 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_4011 | | Putative sensory histidine kinase YfhA | sig. | 39 | 69 |
| | Pseudomonas segetis P6 | ACVTMO_RS19460 | | sigma-54-dependent transcriptional regulator | cofit | 39 | 69 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS03885 | norR;go_function=DNA-binding | nitric oxide reductase transcriptional regulator NorR | insig. | 39 | 71 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_13385 | | nitric oxide reductase transcriptional regulator NorR | sig. | 39 | 71 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS34920 | norR;go_function=DNA-binding | nitric oxide reductase transcriptional regulator NorR | insig. | 39 | 71 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01307 | | Transcriptional regulator of acetoin/glycerol metabolism | insig. | 38 | 64 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS06965 | fleQ;inference=COORDINATES | transcriptional regulator FleQ | strong | 38 | 78 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_05045 | | transcriptional regulator FleQ | strong | 38 | 78 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS45870 | fleQ;inference=COORDINATES | transcriptional regulator FleQ | strong | 38 | 78 |
| | Shewanella oneidensis MR-1 | SO3232 | flrA | flagellar regulatory protein A (NCBI ptt file) | strong | 37 | 68 |
| | Shewanella loihica PV-4 | Shew_1362 | | sigma-54 dependent trancsriptional regulator (RefSeq) | strong | 37 | 68 |
| | Pseudomonas segetis P6 | ACVTMO_RS21260 | ntrC | nitrogen regulation protein NR(I) | strong | 36 | 71 |
| | Shewanella sp. ANA-3 | Shewana3_2849 | | sigma-54 dependent trancsriptional regulator (RefSeq) | insig. | 36 | 75 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS23315 | | sigma-54-dependent Fis family transcriptional regulator | strong | 34 | 86 |
| | Ralstonia solanacearum UW163 | UW163_RS16565 | | sigma-54-dependent Fis family transcriptional regulator | strong | 34 | 86 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_4350 | | nitrogen regulation protein NR(I) | insig. | 34 | 86 |
| | Acinetobacter baumannii LAC-4 | RR41_RS08030 | | nitrogen regulation protein NR(I) | insig. | 33 | 86 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_1363 | | PEP-CTERM-box response regulator transcription factor | strong | 39 | 72 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2114 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | insig. | 40 | 69 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF5269 | | Nitrogen regulation protein NR(I) | insig. | 39 | 72 |
| | Sphingobium sp. HT1-2 | GFF1148 | | Nitrogen regulation protein NR(I), GlnG (=NtrC) | strong | 38 | 67 |