| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_04320 | | type 4 prepilin-like proteins leader peptide-processing enzyme | insig. | 100 | 100 |
| | Lysobacter sp. OAE881 | ABIE51_RS04805 | | A24 family peptidase | insig. | 31 | 95 |
| | Erwinia tracheiphila SCR3 | LU632_RS25275 | | A24 family peptidase | insig. | 29 | 98 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_09735 | | Prepilin leader peptidase/N-methyltransferase | cofit | 29 | 98 |
| | Escherichia coli ECRC62 | BNILDI_00555 | pppA | prepilin peptidase PppA | insig. | 31 | 96 |
| | Escherichia coli BL21 | ECD_02841 | | bifunctional prepilin leader peptidase/ methylase | strong | 31 | 96 |
| | Escherichia coli ECOR38 | HEPCGN_18035 | pppA | prepilin peptidase PppA | strong | 31 | 96 |
| | Escherichia coli ECOR27 | NOLOHH_10960 | pppA | prepilin peptidase PppA | insig. | 31 | 96 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS17100 | | prepilin peptidase PppA | insig. | 31 | 96 |
| | Pseudomonas putida KT2440 | PP_0632 | | leader peptidase/N-methyltransferase | insig. | 31 | 84 |
| | Pseudomonas orientalis W4I3 | QF045_RS06875 | | A24 family peptidase | insig. | 28 | 97 |
| | Escherichia fergusonii Becca | EFB2_00842 | | Prepilin peptidase PppA | insig. | 31 | 96 |
| | Escherichia coli BW25113 | b2972 | b2972 | putative peptidase (VIMSS) | insig. | 30 | 96 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00736 | | Leader peptidase PppA | insig. | 30 | 96 |
| | Pseudomonas sp. BP01 | JOY50_RS27200 | | A24 family peptidase | insig. | 29 | 94 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_5467 | | Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-) | insig. | 29 | 97 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_4764 | | Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-) | no data | 31 | 88 |
| | Rahnella sp. WP5 | EX31_RS16215 | | prepilin peptidase | insig. | 31 | 97 |
| | Escherichia coli ECOR27 | NOLOHH_24515 | | prepilin peptidase | insig. | 30 | 94 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS27315 | | A24 family peptidase | cofit | 26 | 96 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS03940 | | prepilin peptidase | insig. | 28 | 98 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS03940 | | prepilin peptidase | sig. | 28 | 98 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_12285 | | prepilin peptidase | no data | 29 | 95 |
| | Klebsiella michiganensis M5al | BWI76_RS05035 | | prepilin peptidase | no data | 30 | 94 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS06190 | | prepilin peptidase | cofit | 30 | 83 |
| | Ralstonia solanacearum UW163 | UW163_RS07435 | | prepilin peptidase | insig. | 30 | 83 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_1508 | | Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-) | insig. | 29 | 97 |
| | Methylophilus sp. DMC18 | GFF847 | | Type 4 prepilin-like proteins leader peptide-processing enzyme | cofit | 29 | 93 |
| | Pseudomonas simiae WCS417 | PS417_03895 | | methyltransferase | strong | 29 | 85 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_13290 | | prepilin peptidase | insig. | 30 | 91 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.1345.1 | | Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-) | no data | 28 | 94 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_2140 | | type 4 prepilin peptidase 1 (EC:3.4.23.43). Aspartic peptidase. MEROPS family A24A | no data | 31 | 82 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS10050 | | A24 family peptidase | strong | 27 | 95 |
| | Rahnella sp. WP5 | EX31_RS12560 | | prepilin peptidase | insig. | 30 | 97 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS17155 | | peptidase A24 | sig. | 28 | 96 |
| | Alteromonas macleodii MIT1002 | MIT1002_03011 | | Pectic enzymes secretion protein OutO | no data | 33 | 91 |
| | Dickeya dadantii 3937 | DDA3937_RS14750 | | prepilin peptidase | insig. | 27 | 96 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1281 | | Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-) | cofit | 28 | 88 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06255 | | leader peptidase (prepilin peptidase) / N-methyltransferase | sig. | 26 | 98 |
| | Erwinia amylovora T8 | OLJFJH_01160 | | prepilin peptidase | insig. | 33 | 87 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS01855 | | A24 family peptidase | strong | 30 | 77 |
| | Dickeya dianthicola 67-19 | HGI48_RS14685 | | prepilin peptidase | insig. | 27 | 91 |
| | Dickeya dianthicola ME23 | DZA65_RS15630 | | prepilin peptidase | insig. | 27 | 91 |
| | Paraburkholderia graminis OAS925 | ABIE53_003596 | | leader peptidase (prepilin peptidase)/N-methyltransferase | strong | 28 | 82 |
| | Pectobacterium carotovorum WPP14 | HER17_RS06740 | | A24 family peptidase | insig. | 29 | 83 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_0796 | | type 4 prepilin peptidase 1, Aspartic peptidase, MEROPS family A24A | strong | 30 | 82 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_0796 | | type 4 prepilin peptidase 1, Aspartic peptidase, MEROPS family A24A | cofit | 30 | 82 |
| | Dechlorosoma suillum PS | Dsui_1318 | | prepilin signal peptidase PulO-like peptidase | strong | 30 | 83 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS05925 | | A24 family peptidase | cofit | 31 | 78 |
| | Ralstonia solanacearum GMI1000 | RS_RS14140 | | prepilin peptidase | sig. | 31 | 77 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS11050 | | prepilin peptidase | sig. | 29 | 77 |
| | Rhodanobacter sp. FW510-T8 | OKGIIK_10830 | pulO | prepilin peptidase | sig. | 30 | 78 |
| | Acinetobacter radioresistens SK82 | MPMX26_00244 | | Type 4 prepilin-like proteins leader peptide-processing enzyme | cofit | 30 | 84 |
| | Pseudomonas sp. S08-1 | OH686_16495 | | Leader peptidase (Prepilin peptidase) / N-methyltransferase | cofit | 31 | 85 |
| | Marinobacter adhaerens HP15 | HP15_2411 | | type IV pilus prepilin peptidase PilD | strong | 30 | 82 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_2840 | | Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-) | insig. | 29 | 85 |
| | Herbaspirillum seropedicae SmR1 | HSERO_RS01760 | | methyltransferase | insig. | 29 | 88 |
| | Escherichia coli ECRC100 | OKFHMN_00235 | pulO | prepilin peptidase | insig. | 29 | 97 |
| | Escherichia coli ECRC101 | MCAODC_15315 | pulO | prepilin peptidase | insig. | 29 | 97 |
| | Escherichia coli ECRC102 | NIAGMN_28555 | pulO | prepilin peptidase | insig. | 29 | 97 |
| | Escherichia coli ECRC98 | JDDGAC_30345 | pulO | prepilin peptidase | insig. | 29 | 97 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS17990 | | methyltransferase | strong | 29 | 83 |
| | Variovorax sp. OAS795 | ABID97_RS23440 | | A24 family peptidase | strong | 31 | 71 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_00983 | | Type 4 prepilin-like proteins leader peptide-processing enzyme | insig. | 28 | 97 |
| | Enterobacter sp. TBS_079 | MPMX20_01525 | | Type 4 prepilin-like proteins leader peptide-processing enzyme | insig. | 27 | 98 |
| | Shewanella sp. ANA-3 | Shewana3_0417 | | type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A (RefSeq) | strong | 27 | 85 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_2069 | | Type II secretory pathway, prepilin signal peptidase PulO and related peptidases | insig. | 26 | 96 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_16005 | | methyltransferase | strong | 27 | 79 |
| | Shewanella oneidensis MR-1 | SO0414 | pilD | type 4 prepilin-like proteins leader peptide processing enzyme (NCBI ptt file) | strong | 26 | 95 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_14320 | | methyltransferase | insig. | 28 | 82 |
| | Variovorax sp. SCN45 | GFF2508 | | Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-) | strong | 26 | 83 |
| | Shewanella loihica PV-4 | Shew_3440 | | prepilin peptidase (RefSeq) | strong | 29 | 79 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5140 | | Leader peptidase (Prepilin peptidase) (EC 3.4.23.43) / N-methyltransferase (EC 2.1.1.-) | insig. | 27 | 79 |
| | Pseudomonas sp. RS175 | PFR28_03733 | | Type 4 prepilin-like proteins leader peptide-processing enzyme | sig. | 28 | 76 |
| | Pseudomonas stutzeri RCH2 | Psest_3286 | | Type II secretory pathway, prepilin signal peptidase PulO and related peptidases | strong | 29 | 83 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS03730 | | A24 family peptidase | strong | 31 | 85 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS03960 | | A24 family peptidase | strong | 31 | 85 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS23825 | pilD;inference=COORDINATES | type IV prepilin peptidase/methyltransferase PilD | strong | 29 | 82 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS56285 | pilD;inference=COORDINATES | type IV prepilin peptidase/methyltransferase PilD | strong | 29 | 82 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_01435 | | type IV prepilin peptidase/methyltransferase PilD | strong | 29 | 82 |
| | Synechocystis sp000284455 PCC 6803 | SGL_RS02190 | | A24 family peptidase | no data | 32 | 80 |
| | Pseudomonas sp. DMC3 | GFF4016 | | Type 4 prepilin-like proteins leader peptide-processing enzyme | insig. | 30 | 82 |
| | Synechococcus elongatus PCC 7942 | Synpcc7942_1935 | pilD | type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A | strong | 31 | 79 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU1263 | pppA | type IV prepilin-like proteins leader peptidase (TIGR) | strong | 30 | 90 |
| | Caulobacter crescentus NA1000 | CCNA_00183 | | type II secretory pathway, prepilin signal peptidase | insig. | 27 | 83 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_00183 | | type II secretory pathway, prepilin signal peptidase | cofit | 27 | 83 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS07760 | | A24 family peptidase | insig. | 26 | 81 |
| | Pantoea sp. MT58 | IAI47_02105 | | prepilin peptidase | insig. | 25 | 80 |
| | Pseudomonas sp. SVBP6 | COO64_RS11135 | | A24 family peptidase | insig. | 27 | 79 |
| | Shewanella amazonensis SB2B | Sama_0367 | | prepilin peptidase (RefSeq) | strong | 29 | 79 |
| | Pseudomonas segetis P6 | ACVTMO_RS19360 | | prepilin peptidase | cofit | 29 | 82 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_15265 | | Prepilin leader peptidase/N-methyltransferase | cofit | 23 | 84 |
| | Erwinia tracheiphila SCR3 | LU632_RS19880 | | A24 family peptidase | insig. | 24 | 69 |
| | Sphingobium sp. HT1-2 | GFF3508 | | peptidase A24A, prepilin type IV | cofit | 26 | 82 |
| | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS22350 | | prepilin peptidase | insig. | 23 | 79 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1037 | | peptidase A24A domain protein (RefSeq) | sig. | 29 | 64 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_1485 | | Type II secretory pathway, prepilin signal peptidase PulO and related peptidases | insig. | 24 | 90 |
| | Serratia liquefaciens MT49 | IAI46_15620 | | prepilin peptidase | insig. | 31 | 34 |
| | Acinetobacter baumannii LAC-4 | RR41_RS20120 | | A24 family peptidase | insig. | 30 | 42 |
| | Escherichia fergusonii Becca | EFB2_00488 | | Type 4 prepilin-like proteins leader peptide-processing enzyme | insig. | 29 | 38 |