| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Variovorax sp. SCN45 | GFF3328 | | NADH:flavin oxidoreductases, Old Yellow Enzyme family | insig. | 100 | 100 |
| o | Variovorax sp. OAS795 | ABID97_RS02870 | | alkene reductase | insig. | 92 | 100 |
| o | Acidovorax sp. GW101-3H11 | Ac3H11_4975 | | Oxidoreductase, FMN-binding | insig. | 75 | 100 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4802 | | Oxidoreductase, FMN-binding | insig. | 73 | 100 |
| o | Agrobacterium fabrum C58 | Atu2188 | | oxidoreductase | insig. | 66 | 100 |
| o | Rhizobium sp. OAE497 | ABIE40_RS12885 | | alkene reductase | cofit | 66 | 100 |
| o | Sinorhizobium meliloti 1021 | SMc01227 | | glycerol trinitrate (GTN) reductase | insig. | 68 | 100 |
| o | Xanthobacter sp. DMC5 | GFF2396 | | N-ethylmaleimide reductase | insig. | 63 | 99 |
| o | Dickeya dianthicola 67-19 | HGI48_RS14010 | | alkene reductase | insig. | 60 | 99 |
| o | Rhodopseudomonas palustris CGA009 | TX73_022825 | | alkene reductase | insig. | 60 | 99 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS16260 | | alkene reductase | insig. | 61 | 99 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS16260 | | alkene reductase | insig. | 61 | 99 |
| o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_05420 | | N-ethylmaleimide reductase | insig. | 60 | 99 |
| o | Dickeya dianthicola ME23 | DZA65_RS04670 | | alkene reductase | insig. | 59 | 99 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS25605 | | N-ethylmaleimide reductase | insig. | 60 | 99 |
| | Agrobacterium fabrum C58 | Atu5445 | | oxidoreductase | insig. | 59 | 99 |
| o | Pantoea sp. MT58 | IAI47_20855 | | alkene reductase | insig. | 59 | 99 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS34035 | | N-ethylmaleimide reductase | insig. | 60 | 99 |
| o | Rahnella sp. WP5 | EX31_RS11060 | | alkene reductase | insig. | 59 | 99 |
| o | Azospirillum sp. SherDot2 | MPMX19_04479 | | N-ethylmaleimide reductase | insig. | 61 | 99 |
| o | Pseudomonas sp. SVBP6 | COO64_RS08580 | | alkene reductase | insig. | 60 | 99 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS07505 | | alkene reductase | insig. | 60 | 99 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_28430 | | N-ethylmaleimide reductase | insig. | 60 | 99 |
| o | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.3623.1 | | 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34) | insig. | 58 | 99 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_1653 | | NADH:flavin oxidoreductase/NADH oxidase | insig. | 59 | 99 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_28605 | | N-ethylmaleimide reductase | insig. | 58 | 99 |
| o | Pectobacterium carotovorum WPP14 | HER17_RS14310 | | alkene reductase | cofit | 59 | 99 |
| o | Bosea sp. OAE506 | ABIE41_RS08040 | | alkene reductase | strong | 61 | 99 |
| o | Dechlorosoma suillum PS | Dsui_1877 | | NADH:flavin oxidoreductase | cofit | 60 | 100 |
| o | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS14410 | | alkene reductase | cofit | 57 | 99 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_02317 | | N-ethylmaleimide reductase | insig. | 59 | 99 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_4194 | | 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34) | insig. | 57 | 99 |
| o | Herbaspirillum seropedicae SmR1 | HSERO_RS15610 | | NADH:flavin oxidoreductase | insig. | 56 | 99 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS10215 | | N-ethylmaleimide reductase | insig. | 54 | 99 |
| | Azospirillum sp. SherDot2 | MPMX19_01981 | | N-ethylmaleimide reductase | insig. | 56 | 99 |
| o | Pseudomonas orientalis W4I3 | QF045_RS20930 | | alkene reductase | insig. | 55 | 98 |
| | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS21280 | | alkene reductase | cofit | 55 | 98 |
| o | Pseudomonas syringae pv. syringae B728a | Psyr_2934 | | NADH:flavin oxidoreductase/NADH oxidase | insig. | 55 | 98 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_2934 | | NADH:flavin oxidoreductase/NADH oxidase | insig. | 55 | 98 |
| o | Methylophilus sp. DMC18 | GFF937 | | N-ethylmaleimide reductase | insig. | 53 | 99 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS15460 | | alkene reductase | insig. | 54 | 98 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS15460 | | alkene reductase | insig. | 54 | 98 |
| o | Azospirillum brasilense Sp245 | AZOBR_RS04305 | | 1 2-oxophytodienoate reductase | insig. | 51 | 99 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS27240 | | N-ethylmaleimide reductase | insig. | 50 | 100 |
| | Azospirillum sp. SherDot2 | MPMX19_00474 | | N-ethylmaleimide reductase | insig. | 52 | 99 |
| o | Ralstonia solanacearum PSI07 | RPSI07_RS18165 | | alkene reductase | insig. | 51 | 99 |
| o | Ralstonia solanacearum GMI1000 | RS_RS06020 | | alkene reductase | insig. | 51 | 99 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06090 | | N-ethylmaleimide reductase | insig. | 49 | 100 |
| o | Rhodospirillum rubrum S1H | Rru_A0475 | | NADH:flavin oxidoreductase/NADH oxidase (NCBI) | cofit | 52 | 99 |
| o | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS20595 | | alkene reductase | insig. | 51 | 99 |
| o | Ralstonia solanacearum UW163 | UW163_RS18580 | | alkene reductase | insig. | 51 | 99 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS10850 | | alkene reductase | insig. | 51 | 99 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_25340 | | NADH:flavin oxidoreductase | insig. | 47 | 100 |
| o | Synechococcus elongatus PCC 7942 | Synpcc7942_0473 | | NADH:flavin oxidoreductase Old Yellow Enzyme family-like | insig. | 51 | 99 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_1345 | | NADH:flavin oxidoreductases, Old Yellow Enzyme family | insig. | 47 | 100 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS25465 | | 1,2-oxophytodienoate reductase | insig. | 48 | 99 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS22530 | | NADH:flavin oxidoreductase | insig. | 51 | 100 |
| o | Pseudomonas stutzeri RCH2 | Psest_2814 | | NADH:flavin oxidoreductases, Old Yellow Enzyme family | sig. | 50 | 100 |
| | Dickeya dianthicola 67-19 | HGI48_RS12730 | | alkene reductase | insig. | 48 | 99 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_00058 | | N-ethylmaleimide reductase | insig. | 49 | 100 |
| o | Paraburkholderia sabiae LMG 24235 | QEN71_RS33520 | | alkene reductase | insig. | 51 | 99 |
| o | Pseudomonas sp. DMC3 | GFF2806 | | N-ethylmaleimide reductase | cofit | 48 | 98 |
| o | Paraburkholderia graminis OAS925 | ABIE53_005302 | | 2,4-dienoyl-CoA reductase-like NADH-dependent reductase (Old Yellow Enzyme family) | strong | 50 | 100 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS06385 | | N-ethylmaleimide reductase | insig. | 49 | 99 |
| o | Serratia liquefaciens MT49 | IAI46_11280 | | alkene reductase | insig. | 49 | 100 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_03333 | | hypothetical protein | insig. | 49 | 100 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS20745 | | alkene reductase | insig. | 51 | 100 |
| o | Pseudomonas segetis P6 | ACVTMO_RS18580 | | alkene reductase | insig. | 49 | 100 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS20375 | | NADH-flavin oxidoreductase / NADH oxidase | insig. | 50 | 99 |
| o | Pseudomonas sp. RS175 | PFR28_01439 | | N-ethylmaleimide reductase | insig. | 49 | 98 |
| o | Pseudomonas putida KT2440 | PP_0920 | | Xenobiotic reductase B | insig. | 49 | 100 |
| | Pseudomonas stutzeri RCH2 | Psest_0753 | | NADH:flavin oxidoreductases, Old Yellow Enzyme family | insig. | 46 | 99 |
| o | Dickeya dadantii 3937 | DDA3937_RS14735 | | alkene reductase | insig. | 49 | 100 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_17320 | | NADH:flavin oxidoreductase | strong | 48 | 100 |
| o | Vibrio cholerae E7946 ATCC 55056 | CSW01_19080 | | alkene reductase | insig. | 48 | 100 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS28780 | | NADH:flavin oxidoreductase | insig. | 49 | 100 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01518 | | N-ethylmaleimide reductase | cofit | 48 | 100 |
| o | Pseudomonas aeruginosa MRSN321 | DY961_RS19015 | | alkene reductase | sig. | 47 | 99 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_06345 | | alkene reductase | sig. | 47 | 99 |
| o | Pseudomonas aeruginosa PUPa3 | DQ20_RS53145 | | alkene reductase | sig. | 47 | 99 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_3250 | | NADH:flavin oxidoreductases, Old Yellow Enzyme family | insig. | 47 | 100 |
| o | Pseudomonas simiae WCS417 | PS417_06785 | | NADH:flavin oxidoreductase | insig. | 47 | 100 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS24715 | | alkene reductase | insig. | 49 | 100 |
| o | Pseudomonas sp. BP01 | JOY50_RS14240 | | alkene reductase | insig. | 48 | 100 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS38950 | | alkene reductase | insig. | 47 | 99 |
| o | Acinetobacter baumannii LAC-4 | RR41_RS00940 | | alkene reductase | insig. | 47 | 100 |
| | Dickeya dianthicola 67-19 | HGI48_RS14670 | | alkene reductase | insig. | 47 | 100 |
| | Dickeya dianthicola ME23 | DZA65_RS15615 | | alkene reductase | insig. | 48 | 100 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS02325 | | alkene reductase | insig. | 47 | 99 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_14895 | | alkene reductase | insig. | 47 | 99 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS33265 | | alkene reductase | insig. | 47 | 99 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS57165 | | alkene reductase | insig. | 48 | 100 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_02325 | | alkene reductase | insig. | 48 | 100 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS06845 | | alkene reductase | insig. | 47 | 100 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS06845 | | alkene reductase | insig. | 47 | 100 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_4053 | | NADH:flavin oxidoreductase/NADH oxidase | insig. | 48 | 100 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_4053 | | NADH:flavin oxidoreductase/NADH oxidase | insig. | 48 | 100 |
| | Rahnella sp. WP5 | EX31_RS13805 | | alkene reductase | insig. | 47 | 100 |
| | Pseudomonas sp. DMC3 | GFF2215 | | N-ethylmaleimide reductase | cofit | 47 | 100 |
| o | Alteromonas macleodii MIT1002 | MIT1002_01213 | | N-ethylmaleimide reductase | insig. | 46 | 98 |