| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Pseudomonas syringae pv. syringae B728a | Psyr_4393 | | type II secretion system protein E | sig. | 100 | 100 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_4393 | | type II secretion system protein E | sig. | 100 | 100 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_13820 | | ATPase | insig. | 86 | 99 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_1278 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 86 | 99 |
| o | Pseudomonas sp. DMC3 | GFF3341 | | hypothetical protein | cofit | 86 | 99 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS03175 | | CpaF family protein | insig. | 87 | 99 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS03175 | | CpaF family protein | strong | 87 | 99 |
| o | Pseudomonas simiae WCS417 | PS417_03095 | | ATPase | sig. | 87 | 99 |
| o | Pseudomonas sp. SVBP6 | COO64_RS18465 | | CpaF family protein | insig. | 81 | 100 |
| o | Pseudomonas segetis P6 | MPMX49_04040 | | hypothetical protein | insig. | 75 | 100 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_02600 | | type 4b pilus Flp biogenesis ATPase TadA | insig. | 72 | 96 |
| o | Marinobacter adhaerens HP15 | HP15_p187g9 | | type II secretion system protein E | strong | 54 | 91 |
| o | Ralstonia solanacearum PSI07 | RPSI07_RS05045 | | CpaF family protein | insig. | 51 | 91 |
| o | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS18510 | | CpaF family protein | insig. | 50 | 91 |
| o | Ralstonia solanacearum UW163 | UW163_RS20620 | | CpaF family protein | insig. | 50 | 91 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS35985 | | pilus assembly protein CpaF | sig. | 48 | 95 |
| o | Ralstonia solanacearum GMI1000 | RS_RS22355 | | CpaF family protein | insig. | 50 | 91 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS19545 | | CpaF family protein | strong | 49 | 92 |
| o | Paraburkholderia sabiae LMG 24235 | QEN71_RS10645 | | CpaF family protein | insig. | 50 | 91 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS08185 | | pilus assembly protein CpaF | insig. | 50 | 90 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4121 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | no data | 49 | 89 |
| o | Paraburkholderia graminis OAS925 | ABIE53_002438 | | pilus assembly protein CpaF | cofit | 48 | 94 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS22920 | | pilus assembly protein CpaF | insig. | 48 | 95 |
| | Ralstonia solanacearum GMI1000 | RS_RS03280 | | CpaF family protein | insig. | 49 | 95 |
| o | Variovorax sp. OAS795 | ABID97_RS24290 | | CpaF family protein | no data | 49 | 90 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS04100 | | pilus assembly protein CpaF | insig. | 48 | 92 |
| o | Variovorax sp. SCN45 | GFF2208 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 49 | 90 |
| | Paraburkholderia graminis OAS925 | ABIE53_004998 | | pilus assembly protein CpaF | sig. | 48 | 95 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS12005 | | CpaF family protein | insig. | 50 | 83 |
| | Rhodopseudomonas palustris CGA009 | TX73_019075 | | CpaF family protein | insig. | 48 | 86 |
| o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06368 | | pilus assembly protein CpaF | no data | 48 | 95 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS08500 | | CpaF family protein | insig. | 49 | 94 |
| | Pseudomonas sp. SVBP6 | COO64_RS23430 | | CpaF family protein | insig. | 53 | 87 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2738 | | type II secretion system protein E (RefSeq) | insig. | 51 | 83 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_496 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 54 | 84 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS08110 | | CpaF family protein | insig. | 52 | 81 |
| | Caulobacter crescentus NA1000 | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 51 | 81 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 51 | 81 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_01570 | | pilus assembly protein CpaF | insig. | 53 | 84 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS18950 | | CpaF family protein | insig. | 46 | 89 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5915 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 54 | 80 |
| | Pseudomonas sp. RS175 | PFR28_03044 | | hypothetical protein | insig. | 56 | 78 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_19980 | | pilus assembly protein CpaF | insig. | 54 | 80 |
| | Agrobacterium fabrum C58 | Atu0218 | | component of type IV pilus | insig. | 44 | 93 |
| | Rhizobium sp. OAE497 | ABIE40_RS00885 | | CpaF family protein | insig. | 48 | 82 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS12510 | | CpaF family protein | insig. | 46 | 95 |
| | Ralstonia solanacearum UW163 | UW163_RS01055 | | CpaF family protein | insig. | 46 | 95 |
| | Sinorhizobium meliloti 1021 | SMc02820 | | pilus assembly protein | insig. | 49 | 82 |
| o | Bosea sp. OAE506 | ABIE41_RS03685 | | CpaF family protein | insig. | 43 | 96 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c06110 | | Flp pilus assembly protein, ATPase CpaF | insig. | 51 | 79 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2070 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 53 | 84 |
| | Pectobacterium carotovorum WPP14 | HER17_RS17705 | | CpaF family protein | insig. | 50 | 85 |
| | Shewanella loihica PV-4 | Shew_0644 | | FHA domain-containing protein (RefSeq) | insig. | 50 | 78 |
| | Pseudomonas sp. S08-1 | OH686_02520 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 54 | 87 |
| | Shewanella amazonensis SB2B | Sama_0708 | | type II/IV secretion system protein E (RefSeq) | insig. | 48 | 78 |
| | Sinorhizobium meliloti 1021 | SMa1568 | | CpaF2 pilus assembly protein | insig. | 48 | 81 |
| | Variovorax sp. OAS795 | ABID97_RS09105 | | CpaF family protein | insig. | 49 | 80 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS20760 | | CpaF family protein | insig. | 48 | 95 |
| | Pseudomonas sp. S08-1 | OH686_19900 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 49 | 83 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS34040 | | hypothetical protein | insig. | 50 | 79 |
| | Dinoroseobacter shibae DFL-12 | Dshi_1126 | | type II secretion system protein E (RefSeq) | insig. | 48 | 91 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2122 | cpaF | type II/IV secretion system protein (TIGR) | insig. | 51 | 83 |
| | Pseudomonas stutzeri RCH2 | Psest_2619 | | Flp pilus assembly protein, ATPase CpaF | insig. | 55 | 80 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS17490 | | ATPase, T2SS/T4P/T4SS family | insig. | 45 | 90 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS04365 | | ATPase, T2SS/T4P/T4SS family | insig. | 53 | 78 |
| | Sphingobium sp. HT1-2 | GFF4554 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | cofit | 47 | 81 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0975 | | Flp pilus assembly protein, ATPase CpaF | insig. | 47 | 81 |
| | Mycobacterium tuberculosis H37Rv | Rv3659c | | Conserved hypothetical protein | insig. | 41 | 71 |
| | Methanococcus maripaludis JJ | MMJJ_RS05235 | | Flp pilus assembly complex ATPase component TadA | insig. | 39 | 81 |
| | Methanococcus maripaludis S2 | MMP_RS00250 | | Flp pilus assembly complex ATPase component TadA | strong | 39 | 81 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0389 | | P-type conjugative transfer ATPase TrbB | insig. | 39 | 72 |
| | Caulobacter crescentus NA1000 | CCNA_02505 | | type IV secretory pathway, VirB11 ATPase | sig. | 35 | 69 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_02505 | | type IV secretory pathway, VirB11 ATPase | cofit | 35 | 69 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2572 | | P-type conjugative transfer ATPase TrbB | insig. | 39 | 71 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2912 | | P-type conjugative transfer ATPase TrbB | insig. | 37 | 72 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_3072 | | P-type conjugative transfer ATPase TrbB | insig. | 37 | 72 |
| | Sphingobium sp. HT1-2 | GFF188 | | Conjugative transfer protein TrbB | cofit | 36 | 72 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS01870 | | P-type DNA transfer ATPase VirB11 | insig. | 38 | 66 |
| | Rhodopseudomonas palustris CGA009 | TX73_021370 | | P-type conjugative transfer ATPase TrbB | insig. | 37 | 71 |
| | Marinobacter adhaerens HP15 | HP15_p42g52 | | type II secretion system protein E | cofit | 34 | 71 |
| | Agrobacterium fabrum C58 | Atu6041 | | conjugal transfer protein | insig. | 32 | 78 |
| | Xanthobacter sp. DMC5 | GFF3638 | | hypothetical protein | insig. | 35 | 72 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS43755 | | ATPase, T2SS/T4P/T4SS family | insig. | 32 | 74 |
| | Xanthobacter sp. DMC5 | GFF2635 | | hypothetical protein | insig. | 35 | 72 |
| | Bifidobacterium breve UCC2003 | BBR_RS10950 | | pilus biosynthesis protein | insig. | 37 | 69 |
| | Rhodopseudomonas palustris CGA009 | TX73_011525 | | P-type conjugative transfer ATPase TrbB | insig. | 35 | 69 |
| | Rhodospirillum rubrum S1H | Rru_A0808 | | type II secretion system protein E (NCBI) | insig. | 34 | 71 |
| | Rhodopseudomonas palustris CGA009 | TX73_021310 | | P-type conjugative transfer ATPase TrbB | insig. | 38 | 60 |
| | Sinorhizobium meliloti 1021 | SMa1302 | | VirB11-like ATPase | insig. | 32 | 69 |
| | Agrobacterium fabrum C58 | Atu5172 | | type IV secretion protein AvhB11 | insig. | 31 | 70 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4435 | | Conjugative transfer protein TrbB | insig. | 32 | 71 |
| | Dechlorosoma suillum PS | Dsui_2579 | | Flp pilus assembly protein, ATPase CpaF | cofit | 32 | 78 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS00155 | | P-type conjugative transfer ATPase TrbB | insig. | 33 | 72 |
| | Dickeya dianthicola 67-19 | HGI48_RS08420 | | P-type conjugative transfer ATPase TrbB | insig. | 34 | 73 |
| | Dickeya dadantii 3937 | DDA3937_RS03995 | | P-type conjugative transfer ATPase TrbB | insig. | 34 | 73 |
| | Escherichia coli ECOR38 | HEPCGN_00330 | virB11 | P-type DNA transfer ATPase VirB11 | insig. | 29 | 64 |
| | Variovorax sp. SCN45 | GFF4849 | | Conjugative transfer protein TrbB | insig. | 34 | 72 |
| | Agrobacterium fabrum C58 | Atu6177 | | component of type IV secretion system | insig. | 33 | 65 |
| | Variovorax sp. SCN45 | GFF6579 | | Conjugative transfer protein TrbB | insig. | 32 | 72 |
| | Methanococcus maripaludis JJ | MMJJ_RS05745 | | type II/IV secretion system ATPase subunit | cofit | 30 | 64 |