| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.1353.1 | | Type IV fimbriae expression regulatory protein PilR | sig. | 100 | 100 |
| o | Lysobacter sp. OAE881 | ABIE51_RS04850 | | sigma-54 dependent transcriptional regulator | strong | 69 | 100 |
| o | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS05020 | | sigma-54 dependent transcriptional regulator | strong | 65 | 100 |
| o | Rhodanobacter denitrificans MT42 | LRK55_RS04800 | | sigma-54 dependent transcriptional regulator | cofit | 65 | 100 |
| o | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS04990 | | sigma-54 dependent transcriptional regulator | cofit | 63 | 100 |
| o | Rhodanobacter sp. FW510-T8 | OKGIIK_01730 | atoC | sigma-54-dependent Fis family transcriptional regulator | insig. | 63 | 100 |
| o | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11070 | | sigma-54 dependent transcriptional regulator | cofit | 64 | 100 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_00790 | | two-component response regulator PilR | strong | 63 | 100 |
| o | Pseudomonas syringae pv. syringae B728a | Psyr_0722 | | Two-component response regulator PilR | insig. | 61 | 100 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_0722 | | Two-component response regulator PilR | insig. | 61 | 100 |
| o | Pseudomonas stutzeri RCH2 | Psest_0705 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | strong | 63 | 100 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_2818 | | Type IV fimbriae expression regulatory protein PilR | insig. | 62 | 100 |
| o | Pseudomonas segetis P6 | MPMX49_03926 | | Regulatory protein AtoC | cofit | 61 | 100 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5122 | | Type IV fimbriae expression regulatory protein PilR | insig. | 62 | 100 |
| o | Pseudomonas sp. RS175 | PFR28_03751 | | Regulatory protein AtoC | insig. | 60 | 100 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_15905 | | transcriptional regulator | insig. | 60 | 100 |
| o | Pseudomonas sp. S08-1 | OH686_16390 | | Type IV fimbriae expression regulatory protein PilR | cofit | 61 | 100 |
| o | Pseudomonas sp. DMC3 | GFF4000 | | Regulatory protein AtoC | cofit | 60 | 100 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_14200 | | transcriptional regulator | insig. | 59 | 100 |
| o | Marinobacter adhaerens HP15 | HP15_523 | | two component, sigma54 specific, transcriptional regulator, Fis family | strong | 59 | 100 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_1489 | | Type IV fimbriae expression regulatory protein PilR | insig. | 58 | 100 |
| o | Dechlorosoma suillum PS | Dsui_0135 | | response regulator with CheY-like receiver, AAA-type ATPase, and DNA-binding domains | cofit | 58 | 100 |
| o | Methylophilus sp. DMC18 | GFF2304 | | Regulatory protein AtoC | cofit | 51 | 100 |
| o | Acinetobacter baumannii LAC-4 | RR41_RS17800 | | sigma-54 dependent transcriptional regulator | insig. | 49 | 100 |
| o | Acinetobacter radioresistens SK82 | MPMX26_00162 | | Regulatory protein AtoC | cofit | 47 | 100 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS01750 | | sigma-54 dependent transcriptional regulator | strong | 48 | 100 |
| o | Ralstonia solanacearum PSI07 | RPSI07_RS11155 | | sigma-54-dependent Fis family transcriptional regulator | cofit | 46 | 99 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS17870 | | Fis family transcriptional regulator | insig. | 48 | 100 |
| o | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS06085 | | sigma-54-dependent Fis family transcriptional regulator | strong | 46 | 100 |
| o | Acidovorax sp. GW101-3H11 | Ac3H11_4019 | | Sigma-54 dependent transcriptional regulator | sig. | 46 | 100 |
| o | Ralstonia solanacearum UW163 | UW163_RS07540 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 45 | 100 |
| o | Variovorax sp. SCN45 | GFF4432 | | Two-component system response regulator protein | strong | 45 | 100 |
| o | Ralstonia solanacearum GMI1000 | RS_RS14035 | | sigma-54-dependent Fis family transcriptional regulator | strong | 54 | 83 |
| o | Variovorax sp. OAS795 | ABID97_RS06415 | | sigma-54 dependent transcriptional regulator | strong | 45 | 100 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF595 | | two component, sigma54 specific, transcriptional regulator, Fis family | strong | 46 | 100 |
| o | Desulfovibrio vulgaris Hildenborough JW710 | DVU1419 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | sig. | 43 | 98 |
| o | Desulfovibrio vulgaris Miyazaki F | DvMF_2109 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 42 | 100 |
| | Methylophilus sp. DMC18 | GFF2815 | | Regulatory protein AtoC | insig. | 42 | 100 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01292 | | Regulatory protein AtoC | cofit | 41 | 100 |
| o | Pseudomonas simiae WCS417 | PS417_05525 | | Fis family transcriptional regulator | strong | 41 | 100 |
| | Klebsiella michiganensis M5al | BWI76_RS01605 | | transcriptional regulatory protein ZraR | insig. | 42 | 97 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 40 | 100 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS05610 | | sigma-54-dependent Fis family transcriptional regulator | strong | 40 | 100 |
| o | Kangiella aquimarina DSM 16071 | B158DRAFT_2527 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | sig. | 40 | 100 |
| | Escherichia fergusonii Becca | EFB2_01635 | | Regulatory protein AtoC | insig. | 40 | 98 |
| | Escherichia coli ECOR38 | HEPCGN_22430 | atoC | acetoacetate metabolism transcriptional regulator AtoC | insig. | 40 | 98 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS12965 | | acetoacetate metabolism transcriptional regulator AtoC | no data | 40 | 98 |
| | Escherichia coli BL21 | ECD_02147 | | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein | insig. | 39 | 98 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01490 | | Regulatory protein AtoC | insig. | 39 | 98 |
| | Escherichia coli BW25113 | b2220 | atoC | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein (NCBI) | insig. | 39 | 98 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_0823 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | insig. | 40 | 99 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_16225 | | Fis family transcriptional regulator | insig. | 39 | 100 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_10090 | | sigma-54-dependent response regulator transcription factor ZraR | insig. | 41 | 97 |
| o | Xanthobacter sp. DMC5 | GFF4834 | | Regulatory protein AtoC | insig. | 41 | 99 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU3220 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | no data | 41 | 94 |
| | Escherichia coli ECOR27 | NOLOHH_05465 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 42 | 97 |
| o | Rhodospirillum rubrum S1H | Rru_A1385 | | Two Component, Sigma54 Specific, Transcriptional Regulator, Fis family (NCBI) | cofit | 42 | 100 |
| | Escherichia fergusonii Becca | EFB2_04606 | | Transcriptional regulatory protein ZraR | insig. | 42 | 97 |
| | Escherichia coli BL21 | ECD_03881 | | fused DNA-binding response regulator in two-component regulatory system with ZraS: response regulator/sigma54 interaction protein | insig. | 42 | 97 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS23090 | | sigma-54-dependent response regulator transcription factor ZraR | insig. | 42 | 97 |
| | Escherichia coli BW25113 | b4004 | zraR | fused DNA-binding response regulator in two-component regulatory system with ZraS: response regulator/sigma54 interaction protein (NCBI) | insig. | 42 | 97 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1260 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | insig. | 40 | 99 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_04045 | | Transcriptional regulatory protein ZraR | insig. | 41 | 97 |
| | Escherichia coli ECRC62 | BNILDI_06055 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 41 | 97 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF4188 | | Response regulator of zinc sigma-54-dependent two-component system | insig. | 43 | 95 |
| | Escherichia coli ECOR38 | HEPCGN_11960 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 41 | 97 |
| | Escherichia coli ECRC100 | OKFHMN_13320 | zraR | sigma-54-dependent response regulator transcription factor ZraR | cofit | 41 | 97 |
| o | Pedobacter sp. GW460-11-11-14-LB5 | CA265_RS09195 | | Fis family transcriptional regulator | strong | 39 | 95 |
| | Escherichia coli ECRC101 | MCAODC_03820 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 41 | 97 |
| | Escherichia coli ECRC102 | NIAGMN_11070 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 41 | 97 |
| | Escherichia coli ECRC99 | KEDOAH_14840 | zraR | sigma-54-dependent response regulator transcription factor ZraR | no data | 41 | 97 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_0215 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 45 | 80 |
| o | Azospirillum sp. SherDot2 | MPMX19_03461 | | Regulatory protein AtoC | insig. | 40 | 100 |
| | Phocaeicola dorei CL03T12C01 | ABI39_RS02120 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 39 | 99 |
| o | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_16520 | | DNA-binding response regulator | insig. | 39 | 99 |
| | Escherichia coli ECRC98 | JDDGAC_16945 | zraR | sigma-54-dependent response regulator transcription factor ZraR | insig. | 41 | 97 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2200 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | no data | 41 | 94 |
| o | Mucilaginibacter yixingensis YX-36 DSM 26809 | ABZR88_RS10015 | | sigma-54 dependent transcriptional regulator | sig. | 39 | 95 |
| | Methylophilus sp. DMC18 | GFF1277 | | Regulatory protein AtoC | insig. | 39 | 100 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2149 | | two component, sigma54 specific, transcriptional regulator, Fis family (RefSeq) | strong | 43 | 93 |
| | Phocaeicola vulgatus CL09T03C04 | HMPREF1058_RS02780 | | sigma-54-dependent Fis family transcriptional regulator | strong | 38 | 99 |
| | Pseudomonas sp. S08-1 | OH686_17260 | | Two-component sensor CbrB: intrcellular carbon:nitrogen balance | strong | 40 | 99 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1195 | | Sigma-54 dependent response regulator | insig. | 40 | 100 |
| | Variovorax sp. SCN45 | GFF3669 | | Two-component system response regulator protein | no data | 39 | 100 |
| | Phocaeicola dorei CL03T12C01 | ABI39_RS17605 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 40 | 93 |
| | Marinobacter adhaerens HP15 | HP15_328 | | two component, sigma54 specific, transcriptional regulator, Fis family | strong | 38 | 98 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU0118 | ntrC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | insig. | 40 | 99 |
| | Phocaeicola vulgatus CL09T03C04 | HMPREF1058_RS03545 | | sigma-54-dependent Fis family transcriptional regulator | strong | 40 | 93 |
| | Bacteroides ovatus ATCC 8483 | BACOVA_04200 | | Sigma-54 interaction domain protein | insig. | 37 | 97 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01779 | | DNA-binding transcriptional response regulator, NtrC family, contains REC, AAA-type ATPase, and a Fis-type DNA-binding domains | insig. | 40 | 100 |
| o | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_4656 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | cofit | 36 | 99 |
| o | Vibrio cholerae E7946 ATCC 55056 | CSW01_07640 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 38 | 99 |
| | Azospirillum sp. SherDot2 | MPMX19_05290 | | Hydrogenase transcriptional regulatory protein hupR1 | insig. | 37 | 98 |
| | Dechlorosoma suillum PS | Dsui_2360 | | response regulator with CheY-like receiver, AAA-type ATPase, and DNA-binding domains | cofit | 45 | 79 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU3305 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | strong | 41 | 100 |
| o | Dickeya dianthicola ME23 | DZA65_RS16815 | | two-component system response regulator GlrR | insig. | 39 | 99 |
| | Alteromonas macleodii MIT1002 | MIT1002_01326 | | Quorum-sensing regulator protein F | insig. | 42 | 80 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS30045 | | ATPase AAA | insig. | 40 | 100 |
| o | Cellulophaga baltica 18 | M666_RS02825 | | sigma-54 dependent transcriptional regulator | cofit | 37 | 99 |
| | Pseudomonas sp. BP01 | JOY50_RS00695 | | sigma-54 dependent transcriptional regulator | insig. | 40 | 98 |