| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF4822 | | Queuosine biosynthesis QueD, PTPS-I | strong | 100 | 100 |
| | Escherichia coli ECOR38 | HEPCGN_19100 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 95 | 100 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_2854 | | queuosine biosynthesis protein QueD | insig. | 93 | 100 |
| | Klebsiella michiganensis M5al | BWI76_RS22775 | | 6-carboxytetrahydropterin synthase QueD | insig. | 94 | 100 |
| | Escherichia fergusonii Becca | EFB2_01152 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 93 | 100 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS15415 | | 6-carboxytetrahydropterin synthase QueD | insig. | 93 | 100 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_03305 | | 6-carboxytetrahydropterin synthase QueD | insig. | 94 | 100 |
| | Escherichia coli BL21 | ECD_02610 | | 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) | insig. | 93 | 100 |
| | Escherichia coli ECOR27 | NOLOHH_12180 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 93 | 100 |
| | Escherichia coli ECRC100 | OKFHMN_20365 | queD | 6-carboxytetrahydropterin synthase QueD | cofit | 93 | 100 |
| | Escherichia coli ECRC101 | MCAODC_10890 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 93 | 100 |
| | Escherichia coli ECRC102 | NIAGMN_18135 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 93 | 100 |
| | Escherichia coli ECRC62 | BNILDI_10730 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 93 | 100 |
| | Escherichia coli ECRC98 | JDDGAC_24030 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 93 | 100 |
| | Escherichia coli ECRC99 | KEDOAH_07760 | queD | 6-carboxytetrahydropterin synthase QueD | no data | 93 | 100 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00968 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 93 | 100 |
| | Escherichia coli BW25113 | b2765 | ygcM | 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) | sig. | 93 | 100 |
| | Enterobacter sp. TBS_079 | MPMX20_03644 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | sig. | 92 | 100 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_17415 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 87 | 99 |
| | Erwinia tracheiphila SCR3 | LU632_RS06395 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 87 | 99 |
| | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS16820 | | 6-carboxytetrahydropterin synthase QueD | cofit | 87 | 99 |
| | Pantoea sp. MT58 | IAI47_04145 | | 6-carboxytetrahydropterin synthase QueD | insig. | 87 | 99 |
| | Erwinia amylovora T8 | OLJFJH_09295 | | 6-carboxytetrahydropterin synthase QueD | insig. | 85 | 99 |
| | Dickeya dianthicola 67-19 | HGI48_RS17030 | | 6-carboxytetrahydropterin synthase QueD | insig. | 84 | 100 |
| | Dickeya dadantii 3937 | DDA3937_RS16965 | | 6-carboxytetrahydropterin synthase QueD | sig. | 85 | 100 |
| | Dickeya dianthicola ME23 | DZA65_RS18105 | | 6-carboxytetrahydropterin synthase QueD | sig. | 81 | 100 |
| | Pectobacterium carotovorum WPP14 | HER17_RS04205 | | 6-carboxytetrahydropterin synthase QueD | insig. | 83 | 100 |
| | Rahnella sp. WP5 | EX31_RS07915 | | 6-carboxytetrahydropterin synthase QueD | insig. | 83 | 98 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_06560 | | 6-carboxytetrahydropterin synthase QueD | insig. | 78 | 100 |
| | Serratia liquefaciens MT49 | IAI46_03680 | | 6-carboxytetrahydropterin synthase QueD | insig. | 78 | 99 |
| | Lysobacter sp. OAE881 | ABIE51_RS17950 | | 6-carboxytetrahydropterin synthase QueD | sig. | 71 | 98 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS10415 | | 6-carboxytetrahydropterin synthase QueD | insig. | 67 | 99 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS10415 | | 6-carboxytetrahydropterin synthase QueD | sig. | 67 | 99 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_02161 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | cofit | 67 | 99 |
| | Pseudomonas simiae WCS417 | PS417_09830 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | sig. | 67 | 99 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_110 | | Queuosine biosynthesis QueD, PTPS-I | insig. | 67 | 99 |
| | Pseudomonas orientalis W4I3 | QF045_RS12330 | | 6-carboxytetrahydropterin synthase QueD | insig. | 67 | 99 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_21260 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 66 | 99 |
| | Pseudomonas sp. DMC3 | GFF1361 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | cofit | 67 | 99 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_37 | | 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I | strong | 66 | 99 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_19760 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 66 | 99 |
| | Pseudomonas sp. RS175 | PFR28_02851 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | insig. | 66 | 99 |
| | Pseudomonas putida KT2440 | PP_2341 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase | sig. | 71 | 99 |
| | Pseudomonas sp. BP01 | JOY50_RS10905 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 69 | 99 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2447 | | 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I | sig. | 65 | 99 |
| | Pseudomonas segetis P6 | ACVTMO_RS10970 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 68 | 99 |
| | Pseudomonas sp. SVBP6 | COO64_RS17240 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 68 | 99 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_3215 | | 6-pyruvoyl tetrahydropterin synthase and hypothetical protein | no data | 67 | 99 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3215 | | 6-pyruvoyl tetrahydropterin synthase and hypothetical protein | no data | 67 | 99 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS03880 | queD;go_function=catalytic | 6-carboxytetrahydropterin synthase QueD | sig. | 68 | 99 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_13390 | | 6-carboxytetrahydropterin synthase QueD | no data | 68 | 99 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS34915 | queD;go_function=catalytic | 6-carboxytetrahydropterin synthase QueD | insig. | 68 | 99 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.4528.1 | | hypothetical protein | insig. | 67 | 98 |
| | Pseudomonas stutzeri RCH2 | Psest_2010 | | queuosine biosynthesis protein QueD | no data | 64 | 99 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS09085 | | 6-carboxytetrahydropterin synthase QueD | insig. | 63 | 98 |
| | Pseudomonas sp. S08-1 | OH686_00030 | | queuosine biosynthesis protein QueD | insig. | 72 | 94 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS13650 | | 6-carboxytetrahydropterin synthase QueD | cofit | 62 | 92 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS02150 | | 6-carboxytetrahydropterin synthase QueD | insig. | 59 | 92 |
| | Rhodanobacter sp. FW510-T8 | OKGIIK_15925 | queD | 6-carboxytetrahydropterin synthase QueD | insig. | 58 | 92 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS09315 | | 6-carboxytetrahydropterin synthase QueD | insig. | 57 | 92 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS09655 | | 6-carboxytetrahydropterin synthase QueD | strong | 57 | 92 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1123 | | queuosine biosynthesis protein QueD (RefSeq) | no data | 54 | 99 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2657 | | 6-pyruvoyl tetrahydrobiopterin synthase, putative (TIGR) | insig. | 51 | 99 |
| | Xanthobacter sp. DMC5 | GFF3475 | | hypothetical protein | insig. | 53 | 87 |
| | Rhodopseudomonas palustris CGA009 | TX73_008525 | | 6-carboxytetrahydropterin synthase QueD | insig. | 47 | 99 |
| | Synechocystis sp000284455 PCC 6803 | SGL_RS15290 | | 6-carboxytetrahydropterin synthase QueD | strong | 48 | 94 |
| | Agrobacterium fabrum C58 | Atu3345 | | 6-pyruvoyl tetrahydrobiopterin synthase | insig. | 36 | 98 |
| | Variovorax sp. SCN45 | GFF2576 | | 6-carboxy-5,6,7,8-tetrahydropterin synthase (EC 4.1.2.50) | cofit | 35 | 88 |
| | Variovorax sp. OAS795 | ABID97_RS07420 | | 6-carboxytetrahydropterin synthase | insig. | 35 | 88 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4632 | | 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I | no data | 38 | 60 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_3826 | | 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I | insig. | 38 | 60 |
| | Sinorhizobium meliloti 1021 | SM_b20939 | | 6-pyruvoyl-tetrahydropterin synthase | insig. | 33 | 98 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_0129 | | 6-pyruvoyl tetrahydropterin synthase and hypothetical protein (RefSeq) | no data | 34 | 93 |
| | Caulobacter crescentus NA1000 | CCNA_03260 | | queuosine biosynthesis protein QueD | strong | 36 | 88 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_03260 | | queuosine biosynthesis protein QueD | cofit | 36 | 88 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU1352 | | 6-pyruvoyl tetrahydrobiopterin synthase, putative (TIGR) | no data | 33 | 86 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS08690 | | 6-carboxytetrahydropterin synthase | no data | 35 | 94 |
| | Cellulophaga baltica 18 | M666_RS09275 | | 6-carboxytetrahydropterin synthase | insig. | 38 | 70 |
| | Parabacteroides merdae CL09T00C40 | HMPREF1078_RS00755 | | 6-carboxytetrahydropterin synthase QueD | no data | 35 | 84 |
| | Synechococcus elongatus PCC 7942 | Synpcc7942_1184 | | 6-pyruvoyl-tetrahydropterin synthase-like protein | strong | 33 | 76 |
| | Phocaeicola dorei CL03T12C01 | ABI39_RS10595 | | 6-carboxytetrahydropterin synthase QueD | sig. | 35 | 80 |