| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Methanococcus maripaludis S2 | MMP_RS01515 | | PINc/VapC family ATPase | sig. | 100 | 100 |
| o | Methanococcus maripaludis JJ | MMJJ_RS03860 | | PINc/VapC family ATPase | insig. | 98 | 100 |
| | Pectobacterium carotovorum WPP14 | HER17_RS17705 | | CpaF family protein | insig. | 30 | 23 |
| | Ralstonia solanacearum GMI1000 | RS_RS03280 | | CpaF family protein | insig. | 30 | 22 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS35985 | | pilus assembly protein CpaF | sig. | 27 | 24 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2122 | cpaF | type II/IV secretion system protein (TIGR) | insig. | 33 | 19 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS18950 | | CpaF family protein | insig. | 29 | 18 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2912 | | P-type conjugative transfer ATPase TrbB | insig. | 27 | 21 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS19545 | | CpaF family protein | strong | 26 | 24 |
| | Synechococcus elongatus PCC 7942 | Synpcc7942_0847 | pilT | twitching motility protein | sig. | 29 | 22 |
| | Caulobacter crescentus NA1000 | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 25 | 25 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_03037 | | pilus assembly ATPase CpaF | cofit | 25 | 25 |
| | Synechocystis sp000284455 PCC 6803 | SGL_RS11545 | | type IV pilus twitching motility protein PilT | no data | 25 | 36 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS18510 | | CpaF family protein | insig. | 25 | 24 |
| | Ralstonia solanacearum UW163 | UW163_RS20620 | | CpaF family protein | insig. | 25 | 24 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS03320 | | P-type DNA transfer ATPase VirB11 | insig. | 24 | 33 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS12510 | | CpaF family protein | insig. | 30 | 22 |
| | Ralstonia solanacearum UW163 | UW163_RS01055 | | CpaF family protein | insig. | 30 | 22 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS08500 | | CpaF family protein | insig. | 28 | 22 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0975 | | Flp pilus assembly protein, ATPase CpaF | insig. | 26 | 27 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS05045 | | CpaF family protein | insig. | 25 | 24 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_2738 | | type II secretion system protein E (RefSeq) | insig. | 31 | 19 |
| | Ralstonia solanacearum GMI1000 | RS_RS22355 | | CpaF family protein | insig. | 25 | 24 |
| | Bosea sp. OAE506 | ABIE41_RS03685 | | CpaF family protein | insig. | 29 | 18 |
| | Sphingobium sp. HT1-2 | GFF188 | | Conjugative transfer protein TrbB | cofit | 26 | 26 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS12005 | | CpaF family protein | insig. | 24 | 25 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS43755 | | ATPase, T2SS/T4P/T4SS family | insig. | 26 | 27 |
| | Sphingobium sp. HT1-2 | GFF4554 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | cofit | 25 | 27 |
| | Sinorhizobium meliloti 1021 | SMc02820 | | pilus assembly protein | insig. | 28 | 18 |
| | Xanthobacter sp. DMC5 | GFF3638 | | hypothetical protein | insig. | 27 | 23 |
| | Rahnella sp. WP5 | EX31_RS16170 | | type II secretion system ATPase GspE | insig. | 24 | 27 |
| | Pseudomonas sp. SVBP6 | COO64_RS23430 | | CpaF family protein | insig. | 29 | 17 |
| | Sinorhizobium meliloti 1021 | SMa1568 | | CpaF2 pilus assembly protein | insig. | 26 | 18 |
| | Synechococcus elongatus PCC 7942 | Synpcc7942_2383 | ircA | Nucleotide binding protein, PINc | no data | 33 | 18 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06368 | | pilus assembly protein CpaF | no data | 30 | 17 |
| | Escherichia coli BL21 | ECD_02837 | | putative type II secretion protein GspE | strong | 28 | 23 |
| | Escherichia coli ECOR27 | NOLOHH_10980 | gspE | general secretion pathway protein E | insig. | 28 | 23 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00740 | | Type II secretion system protein E | cofit | 27 | 23 |
| | Rhodopseudomonas palustris CGA009 | TX73_019075 | | CpaF family protein | insig. | 26 | 18 |
| | Rhizobium sp. OAE497 | ABIE40_RS00885 | | CpaF family protein | insig. | 28 | 18 |
| | Xanthobacter sp. DMC5 | GFF2635 | | hypothetical protein | insig. | 27 | 23 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_3072 | | P-type conjugative transfer ATPase TrbB | insig. | 26 | 27 |
| | Variovorax sp. OAS795 | ABID97_RS09105 | | CpaF family protein | insig. | 26 | 22 |
| | Dickeya dianthicola 67-19 | HGI48_RS14730 | | type II secretion system ATPase GspE | insig. | 25 | 24 |
| | Variovorax sp. SCN45 | GFF2208 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 26 | 21 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_0361 | | Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | insig. | 26 | 30 |
| | Paraburkholderia graminis OAS925 | ABIE53_004998 | | pilus assembly protein CpaF | sig. | 30 | 17 |
| | Dickeya dadantii 3937 | DDA3937_RS14795 | | type II secretion system ATPase GspE | cofit | 25 | 24 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS08110 | | CpaF family protein | insig. | 31 | 17 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS10645 | | CpaF family protein | insig. | 30 | 17 |
| | Pseudomonas sp. RS175 | PFR28_03044 | | hypothetical protein | insig. | 28 | 17 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_01570 | | pilus assembly protein CpaF | insig. | 28 | 17 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_19980 | | pilus assembly protein CpaF | insig. | 28 | 17 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_5915 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 28 | 17 |
| | Escherichia fergusonii Becca | EFB2_00846 | | Type II secretion system protein E | insig. | 28 | 23 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4121 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | no data | 27 | 20 |
| | Agrobacterium fabrum C58 | Atu0218 | | component of type IV pilus | insig. | 27 | 22 |
| | Variovorax sp. OAS795 | ABID97_RS24290 | | CpaF family protein | no data | 26 | 21 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0389 | | P-type conjugative transfer ATPase TrbB | insig. | 25 | 22 |
| | Escherichia coli ECOR38 | HEPCGN_18055 | gspE | general secretion pathway protein E | strong | 28 | 23 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS04100 | | pilus assembly protein CpaF | insig. | 30 | 17 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2070 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 28 | 17 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_496 | | Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily | insig. | 28 | 17 |
| | Rhodopseudomonas palustris CGA009 | TX73_011525 | | P-type conjugative transfer ATPase TrbB | insig. | 28 | 23 |
| | Escherichia coli ECRC100 | OKFHMN_00280 | gspE | type II secretion system ATPase GspE | insig. | 26 | 20 |
| | Escherichia coli ECRC101 | MCAODC_15270 | gspE | type II secretion system ATPase GspE | insig. | 26 | 20 |
| | Escherichia coli ECRC102 | NIAGMN_28600 | gspE | type II secretion system ATPase GspE | cofit | 26 | 20 |
| | Escherichia coli ECRC98 | JDDGAC_30300 | gspE | type II secretion system ATPase GspE | insig. | 26 | 20 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS17490 | | ATPase, T2SS/T4P/T4SS family | insig. | 29 | 17 |
| | Rhodopseudomonas palustris CGA009 | TX73_021370 | | P-type conjugative transfer ATPase TrbB | insig. | 27 | 23 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS22920 | | pilus assembly protein CpaF | insig. | 26 | 22 |
| | Dickeya dianthicola ME23 | DZA65_RS15675 | | type II secretion system ATPase GspE | cofit | 25 | 24 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS34040 | | hypothetical protein | insig. | 27 | 19 |
| | Rhodospirillum rubrum S1H | Rru_A0808 | | type II secretion system protein E (NCBI) | insig. | 29 | 17 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS08185 | | pilus assembly protein CpaF | insig. | 29 | 17 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2572 | | P-type conjugative transfer ATPase TrbB | insig. | 28 | 23 |
| | Dechlorosoma suillum PS | Dsui_1393 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 28 | 23 |
| | Rhodopseudomonas palustris CGA009 | TX73_021310 | | P-type conjugative transfer ATPase TrbB | insig. | 26 | 23 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_13340 | | type II secretion system ATPase GspE | insig. | 26 | 20 |
| | Acinetobacter baumannii LAC-4 | RR41_RS15895 | | type II secretion system ATPase GspE | sig. | 28 | 24 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_1886 | | General secretion pathway protein E | no data | 26 | 28 |
| | Pseudomonas simiae WCS417 | PS417_11300 | | general secretion pathway protein GspE | insig. | 26 | 22 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS11895 | | type II secretion system ATPase GspE | no data | 24 | 28 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS11895 | | type II secretion system ATPase GspE | insig. | 24 | 28 |
| | Paraburkholderia graminis OAS925 | ABIE53_002438 | | pilus assembly protein CpaF | cofit | 29 | 17 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.5198.1 | | General secretion pathway protein E | insig. | 25 | 25 |
| | Pectobacterium carotovorum WPP14 | HER17_RS06690 | | type II secretion system ATPase GspE | insig. | 25 | 24 |
| | Pseudomonas sp. S08-1 | OH686_08925 | | Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 24 | 25 |
| | Dechlorosoma suillum PS | Dsui_1455 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 23 | 40 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_2078 | | type II secretion system protein E | insig. | 27 | 24 |