| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Dechlorosoma suillum PS | Dsui_2594 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 100 | 100 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_1511 | | type II secretion system protein E | insig. | 33 | 71 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_1511 | | type II secretion system protein E | insig. | 33 | 71 |
| | Dechlorosoma suillum PS | Dsui_1279 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | strong | 32 | 66 |
| | Pseudomonas sp. S08-1 | OH686_14715 | | MSHA biogenesis protein MshE | insig. | 37 | 69 |
| | Methylophilus sp. DMC18 | GFF1666 | | Type II secretion system protein E | cofit | 32 | 74 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_01185 | | twitching motility protein PilT | insig. | 29 | 89 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_02820 | | type II secretion system ATPase GspE | sig. | 36 | 66 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS58920 | gspE;go_component=type | type II secretion system ATPase GspE | insig. | 36 | 66 |
| | Pseudomonas simiae WCS417 | PS417_11300 | | general secretion pathway protein GspE | insig. | 35 | 66 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS09100 | gspE;go_component=type | type II secretion system ATPase GspE | insig. | 36 | 66 |
| | Dechlorosoma suillum PS | Dsui_1393 | | type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 36 | 49 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_24795 | | type II secretion system protein GspE | insig. | 35 | 58 |
| | Rahnella sp. WP5 | EX31_RS16170 | | type II secretion system ATPase GspE | insig. | 37 | 64 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS10420 | | type IV pilus twitching motility protein PilT | sig. | 33 | 57 |
| | Pseudomonas sp. DMC3 | GFF2988 | | putative type II secretion system protein HxcR | cofit | 35 | 58 |
| | Rhodanobacter sp. FW510-T8 | OKGIIK_11130 | pilT | twitching motility protein PilT | strong | 32 | 57 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_652 | | General secretion pathway protein E | insig. | 36 | 58 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS15745 | | type II secretion system ATPase GspE | insig. | 34 | 67 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS15745 | | type II secretion system ATPase GspE | insig. | 34 | 67 |
| | Rahnella sp. WP5 | EX31_RS03525 | | type II secretion system protein GspE | insig. | 33 | 66 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS04025 | | type IV pilus twitching motility protein PilT | sig. | 33 | 57 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS04255 | | type IV pilus twitching motility protein PilT | strong | 33 | 57 |
| | Marinobacter adhaerens HP15 | HP15_2290 | | type IV-A pilus assembly ATPase PilB | cofit | 30 | 71 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS11895 | | type II secretion system ATPase GspE | no data | 34 | 66 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS11895 | | type II secretion system ATPase GspE | insig. | 34 | 66 |
| | Synechocystis sp000284455 PCC 6803 | SGL_RS11545 | | type IV pilus twitching motility protein PilT | no data | 33 | 57 |
| | Mucilaginibacter yixingensis YX-36 DSM 26809 | ABZR88_RS02570 | | GspE/PulE family protein | insig. | 41 | 38 |
| | Variovorax sp. SCN45 | GFF5234 | | General secretion pathway protein E / Type II secretion cytoplasmic ATP binding protein (PulE, ATPase) | insig. | 34 | 55 |
| | Synechococcus elongatus PCC 7942 | Synpcc7942_2349 | pilT | twitching motility protein | cofit | 31 | 58 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.943.1 | | General secretion pathway protein E | insig. | 36 | 54 |
| | Pseudomonas sp. RS175 | PFR28_01751 | | putative type II secretion system protein HxcR | insig. | 35 | 58 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS05685 | | type IV pilus twitching motility protein PilT | sig. | 33 | 57 |
| | Vibrio cholerae E7946 ATCC 55056 | CSW01_02135 | | MSHA biogenesis protein MshE | cofit | 36 | 58 |
| | Pseudomonas simiae WCS417 | PS417_18085 | | general secretion pathway protein GspE | insig. | 36 | 45 |
| | Variovorax sp. OAS795 | ABID97_RS06945 | | ATPase, T2SS/T4P/T4SS family | insig. | 34 | 55 |
| | Marinobacter adhaerens HP15 | HP15_416 | | type IV-A pilus assembly ATPase PilB | cofit | 33 | 66 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS19940 | | type II secretion system ATPase GspE | insig. | 36 | 45 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS19940 | | type II secretion system ATPase GspE | insig. | 36 | 45 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_03878 | | Type II secretion system protein E | cofit | 36 | 45 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_3151 | | type II secretion system protein E (GspE) | insig. | 34 | 49 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3151 | | type II secretion system protein E (GspE) | insig. | 34 | 49 |
| | Shewanella sp. ANA-3 | Shewana3_3660 | | type II secretion system protein E (RefSeq) | insig. | 29 | 66 |
| | Shewanella oneidensis MR-1 | SO4109 | mshE | MSHA biogenesis protein MshE (NCBI ptt file) | strong | 29 | 66 |
| | Pseudomonas sp. S08-1 | OH686_04390 | | type II secretion system protein E | cofit | 37 | 55 |
| | Dickeya dianthicola 67-19 | HGI48_RS14730 | | type II secretion system ATPase GspE | insig. | 36 | 55 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.1713.1 | | Twitching motility protein PilT | sig. | 33 | 57 |
| | Pseudomonas sp. S08-1 | OH686_11785 | | type II secretion system protein E | insig. | 42 | 42 |
| | Ralstonia solanacearum GMI1000 | RS_RS15645 | | type II secretion system protein GspE | strong | 38 | 47 |
| | Shewanella loihica PV-4 | Shew_0392 | | type II secretion system protein E (RefSeq) | strong | 30 | 66 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_05845 | gspE | type II secretion system protein GspE | cofit | 33 | 65 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS00570 | | type II secretion system protein GspE | insig. | 36 | 55 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF132 | | Type IV fimbrial assembly, ATPase PilB | insig. | 34 | 64 |
| | Erwinia tracheiphila SCR3 | LU632_RS03030 | gspE | type II secretion system protein GspE | insig. | 33 | 65 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS09515 | | type II secretion system protein GspE | cofit | 38 | 47 |
| | Escherichia coli ECOR38 | HEPCGN_08520 | gspE | type II secretion system protein GspE | insig. | 36 | 55 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_0361 | | Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | insig. | 32 | 51 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_13340 | | type II secretion system ATPase GspE | insig. | 39 | 45 |
| | Variovorax sp. OAS795 | ABID97_RS12075 | | ATPase, T2SS/T4P/T4SS family | insig. | 36 | 56 |
| | Escherichia fergusonii Becca | EFB2_03942 | | Type II secretion system protein E | insig. | 36 | 55 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS07745 | | type II secretion system protein GspE | strong | 38 | 47 |
| | Ralstonia solanacearum UW163 | UW163_RS05880 | | type II secretion system protein GspE | insig. | 38 | 47 |
| | Xanthobacter sp. DMC5 | GFF3108 | | Type II secretion system protein E | insig. | 34 | 55 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS03455 | | type II secretion system ATPase GspE | strong | 38 | 47 |
| | Pseudomonas orientalis W4I3 | QF045_RS19490 | | ATPase, T2SS/T4P/T4SS family | cofit | 34 | 51 |
| | Escherichia fergusonii Becca | EFB2_00497 | | Putative type II secretion system protein E | cofit | 39 | 47 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_3952 | | Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | cofit | 38 | 52 |
| | Erwinia amylovora T8 | OLJFJH_01115 | | type II secretion system ATPase GspE | insig. | 37 | 52 |
| | Caulobacter crescentus NA1000 | CCNA_00173 | | type II secretion pathway protein E | cofit | 36 | 54 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_00173 | | type II secretion pathway protein E | cofit | 36 | 54 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00377 | | Putative type II secretion system protein E | insig. | 35 | 55 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS19065 | | type II secretion system ATPase GspE | insig. | 35 | 55 |
| | Escherichia coli BW25113 | b3326 | gspE | general secretory pathway component, cryptic (NCBI) | insig. | 35 | 55 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_04610 | | twitching motility protein PilT | insig. | 35 | 57 |
| | Dickeya dianthicola ME23 | DZA65_RS15675 | | type II secretion system ATPase GspE | cofit | 35 | 55 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_3343 | | Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB | insig. | 33 | 59 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_13070 | | type II secretion system protein GspE | sig. | 33 | 72 |
| | Azospirillum sp. SherDot2 | MPMX19_00280 | | hypothetical protein | insig. | 30 | 59 |
| | Pectobacterium carotovorum WPP14 | HER17_RS06690 | | type II secretion system ATPase GspE | insig. | 34 | 59 |
| | Rhodanobacter sp. FW510-T8 | OKGIIK_16215 | gspE | type II secretion system ATPase GspE | insig. | 36 | 43 |
| | Dickeya dadantii 3937 | DDA3937_RS14795 | | type II secretion system ATPase GspE | cofit | 36 | 55 |
| | Marinobacter adhaerens HP15 | HP15_289 | | twitching motility protein PilT | strong | 33 | 57 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS23835 | pilB;go_function=ATP | type IV-A pilus assembly ATPase PilB | strong | 32 | 58 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS56295 | pilB;go_function=ATP | type IV-A pilus assembly ATPase PilB | strong | 32 | 58 |
| | Alteromonas macleodii MIT1002 | MIT1002_03060 | | Twitching mobility protein | insig. | 31 | 64 |
| | Escherichia coli BL21 | ECD_03177 | | general secretory pathway component, cryptic | insig. | 41 | 38 |
| | Lysobacter sp. OAE881 | ABIE51_RS16055 | | type IV pilus twitching motility protein PilT | strong | 32 | 57 |
| | Erwinia amylovora T8 | OLJFJH_01055 | | type II secretion system protein GspE | insig. | 36 | 55 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS09960 | | type II secretion system ATPase GspE | insig. | 36 | 38 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_2297 | | Twitching motility protein PilT | insig. | 29 | 61 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS02730 | | type II secretion system ATPase GspE | cofit | 36 | 38 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_01445 | | type 4 fimbrial biogenesis protein PilB | strong | 32 | 58 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS17350 | | twitching motility protein PilT | insig. | 31 | 57 |
| | Escherichia coli BL21 | ECD_00106 | | T2SE secretion family protein; P-loop ATPase superfamily protein | insig. | 35 | 55 |
| | Escherichia coli BW25113 | b0107 | hofB | conserved protein with nucleoside triphosphate hydrolase domain (NCBI) | insig. | 35 | 55 |
| | Escherichia coli ECRC100 | OKFHMN_10540 | gspE | type II secretion system protein GspE | cofit | 35 | 55 |
| | Escherichia coli ECRC101 | MCAODC_01055 | gspE | type II secretion system protein GspE | insig. | 35 | 55 |
| | Escherichia coli ECRC102 | NIAGMN_08390 | gspE | type II secretion system protein GspE | cofit | 35 | 55 |
| | Escherichia coli ECRC99 | KEDOAH_17615 | gspE | type II secretion system protein GspE | no data | 35 | 55 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS11730 | | type IV pili twitching motility protein PilT | strong | 31 | 57 |