| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_2039 | | Sigma-54 dependent transcriptional regulator | sig. | 100 | 100 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_00410 | | Fis family transcriptional regulator | sig. | 97 | 100 |
| o | Pseudomonas sp. RS175 | PFR28_01397 | | Anaerobic nitric oxide reductase transcription regulator NorR | sig. | 95 | 100 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_5631 | | Sigma-54 dependent transcriptional regulator | insig. | 91 | 100 |
| o | Pseudomonas sp. DMC3 | GFF3656 | | Anaerobic nitric oxide reductase transcription regulator NorR | insig. | 90 | 100 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2875 | | Sigma-54 dependent transcriptional regulator | strong | 90 | 100 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_21780 | | Fis family transcriptional regulator | insig. | 89 | 100 |
| o | Pseudomonas simiae WCS417 | PS417_12160 | | Fis family transcriptional regulator | insig. | 83 | 100 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_03364 | | Anaerobic nitric oxide reductase transcription regulator NorR | cofit | 83 | 99 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS12850 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 84 | 99 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS12850 | | sigma-54-dependent Fis family transcriptional regulator | sig. | 84 | 99 |
| o | Pseudomonas sp. SVBP6 | COO64_RS23875 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 79 | 99 |
| o | Pseudomonas sp. S08-1 | OH686_00830 | | Transcriptional regulator | cofit | 75 | 99 |
| o | Pseudomonas putida KT2440 | PP_3075 | | putative transcriptional regulator | strong | 75 | 99 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_09800 | | sigma-54-dependent transcriptional regulator HbcR | insig. | 73 | 99 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS34340 | | Fis family transcriptional regulator | strong | 56 | 97 |
| o | Paraburkholderia graminis OAS925 | ABIE53_004708 | | transcriptional regulator with PAS, ATPase and Fis domain | insig. | 57 | 97 |
| o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_03547 | | Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding Fis domains | insig. | 56 | 97 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS10735 | | Fis family transcriptional regulator | insig. | 57 | 99 |
| o | Paraburkholderia sabiae LMG 24235 | QEN71_RS17125 | | sigma 54-interacting transcriptional regulator | insig. | 57 | 97 |
| o | Azospirillum sp. SherDot2 | MPMX19_06065 | | Anaerobic nitric oxide reductase transcription regulator NorR | cofit | 52 | 97 |
| o | Pseudomonas stutzeri RCH2 | Psest_1071 | | Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains | strong | 50 | 97 |
| o | Azospirillum brasilense Sp245 | AZOBR_RS21185 | | Fis family transcriptional regulator | insig. | 51 | 98 |
| o | Pseudomonas segetis P6 | MPMX49_00786 | | Anaerobic nitric oxide reductase transcription regulator NorR | insig. | 49 | 97 |
| | Azospirillum sp. SherDot2 | MPMX19_05582 | | Anaerobic nitric oxide reductase transcription regulator NorR | insig. | 51 | 98 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF5124 | | Sigma-54 dependent transcriptional regulator | insig. | 48 | 98 |
| o | Marinobacter adhaerens HP15 | HP15_3793 | | protein containing RNA polymerase sigma factor 54, interaction / helix-turn-helix, Fis-type | insig. | 52 | 95 |
| o | Variovorax sp. OAS795 | ABID97_RS17700 | | sigma 54-interacting transcriptional regulator | insig. | 49 | 96 |
| o | Variovorax sp. SCN45 | GFF518 | | Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains | insig. | 48 | 97 |
| o | Acidovorax sp. GW101-3H11 | Ac3H11_63 | | Sigma-54 dependent transcriptional regulator | insig. | 47 | 96 |
| | Azospirillum sp. SherDot2 | MPMX19_03130 | | Anaerobic nitric oxide reductase transcription regulator NorR | insig. | 49 | 95 |
| | Azospirillum brasilense Sp245 | AZOBR_RS26990 | | Fis family transcriptional regulator | insig. | 46 | 97 |
| o | Xanthobacter sp. DMC5 | GFF2822 | | Anaerobic nitric oxide reductase transcription regulator NorR | insig. | 47 | 97 |
| o | Desulfovibrio vulgaris Miyazaki F | DvMF_1933 | | PAS modulated sigma54 specific transcriptional regulator, Fis family (RefSeq) | insig. | 40 | 95 |
| | Pseudomonas stutzeri RCH2 | Psest_1765 | | Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains | strong | 43 | 68 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS11255 | | Fis family transcriptional regulator | strong | 38 | 93 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS30045 | | ATPase AAA | insig. | 41 | 87 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_02520 | | Anaerobic nitric oxide reductase transcription regulator NorR | cofit | 38 | 97 |
| | Escherichia fergusonii Becca | EFB2_01192 | | Formate hydrogenlyase transcriptional activator FhlA | cofit | 45 | 71 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS15260 | | formate hydrogenlyase transcriptional activator FlhA | insig. | 45 | 71 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01779 | | DNA-binding transcriptional response regulator, NtrC family, contains REC, AAA-type ATPase, and a Fis-type DNA-binding domains | insig. | 42 | 82 |
| | Escherichia fergusonii Becca | EFB2_01635 | | Regulatory protein AtoC | insig. | 39 | 76 |
| | Escherichia coli ECOR38 | HEPCGN_22430 | atoC | acetoacetate metabolism transcriptional regulator AtoC | insig. | 39 | 76 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS12965 | | acetoacetate metabolism transcriptional regulator AtoC | no data | 39 | 76 |
| | Pseudomonas sp. S08-1 | OH686_02855 | | Flagellar regulatory protein FleQ | cofit | 43 | 68 |
| | Escherichia coli BL21 | ECD_02147 | | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein | insig. | 39 | 76 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01490 | | Regulatory protein AtoC | insig. | 39 | 76 |
| | Escherichia coli BW25113 | b2220 | atoC | fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein (NCBI) | insig. | 39 | 76 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_3461 | | Helix-turn-helix, Fis-type | strong | 43 | 68 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3461 | | Helix-turn-helix, Fis-type | cofit | 43 | 68 |
| | Pseudomonas sp. SVBP6 | COO64_RS07975 | | sigma-54 dependent transcriptional regulator | insig. | 48 | 66 |
| | Escherichia coli ECRC100 | OKFHMN_20540 | flhA | formate hydrogenlyase transcriptional activator FlhA | insig. | 45 | 71 |
| | Escherichia coli ECRC101 | MCAODC_11065 | flhA | formate hydrogenlyase transcriptional activator FlhA | insig. | 45 | 71 |
| | Escherichia coli ECRC102 | NIAGMN_18310 | flhA | formate hydrogenlyase transcriptional activator FlhA | insig. | 45 | 71 |
| | Escherichia coli ECRC98 | JDDGAC_24205 | flhA | formate hydrogenlyase transcriptional activator FlhA | insig. | 45 | 71 |
| | Escherichia coli ECRC99 | KEDOAH_07595 | flhA | formate hydrogenlyase transcriptional activator FlhA | no data | 45 | 71 |
| | Pseudomonas sp. SVBP6 | COO64_RS21300 | fleQ | transcriptional regulator FleQ | cofit | 43 | 68 |
| | Methylophilus sp. DMC18 | GFF1426 | | Transcriptional regulatory protein ZraR | insig. | 40 | 88 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS01330 | | Fis family transcriptional regulator | insig. | 37 | 93 |
| | Escherichia coli ECRC62 | BNILDI_10935 | flhA | formate hydrogenlyase transcriptional activator FlhA | cofit | 45 | 71 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_10840 | | propionate catabolism operon regulatory protein PrpR | sig. | 36 | 96 |
| | Escherichia coli ECOR27 | NOLOHH_12385 | flhA | formate hydrogenlyase transcriptional activator FlhA | insig. | 45 | 71 |
| | Herbaspirillum seropedicae SmR1 | HSERO_RS23170 | | ATPase AAA | insig. | 47 | 65 |
| | Escherichia coli BL21 | ECD_02581 | | formate hydrogenlyase transcriptional activator | insig. | 45 | 71 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01003 | | Formate hydrogenlyase transcriptional activator FhlA | insig. | 45 | 71 |
| | Escherichia coli BW25113 | b2731 | fhlA | DNA-binding transcriptional activator (NCBI) | insig. | 45 | 71 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_04163 | | Anaerobic nitric oxide reductase transcription regulator NorR | strong | 42 | 68 |
| | Pseudomonas simiae WCS417 | PS417_19825 | | ATPase AAA | strong | 42 | 68 |
| | Variovorax sp. SCN45 | GFF3669 | | Two-component system response regulator protein | no data | 42 | 79 |
| | Marinobacter adhaerens HP15 | HP15_2330 | | two component, sigma54 specific, transcriptional regulator, Fis family | strong | 41 | 70 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS21795 | | sigma-54-dependent Fis family transcriptional regulator | strong | 42 | 68 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS21795 | | sigma-54-dependent Fis family transcriptional regulator | strong | 42 | 68 |
| | Pseudomonas putida KT2440 | PP_4373 | | Transcriptional regulator FleQ | strong | 42 | 68 |
| | Caulobacter crescentus NA1000 | CCNA_03424 | | AAA-family response regulator tacA | strong | 41 | 72 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_03424 | | AAA-family response regulator tacA | cofit | 41 | 72 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_03040 | | formate hydrogenlyase transcriptional activator FlhA | insig. | 46 | 71 |
| | Klebsiella michiganensis M5al | BWI76_RS22370 | | transcriptional regulator FhlA | insig. | 45 | 72 |
| | Pseudomonas sp. BP01 | JOY50_RS00705 | fleQ | transcriptional regulator FleQ | sig. | 42 | 68 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF4725 | | Formate hydrogenlyase transcriptional activator | insig. | 44 | 71 |
| | Pseudomonas sp. DMC3 | GFF2445 | | Anaerobic nitric oxide reductase transcription regulator NorR | cofit | 42 | 68 |
| | Pseudomonas sp. RS175 | PFR28_00828 | | Anaerobic nitric oxide reductase transcription regulator NorR | strong | 42 | 68 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_3599 | | Flagellar regulatory protein FleQ | insig. | 42 | 68 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_03855 | | AAA family ATPase | strong | 42 | 68 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_2597 | | Flagellar regulatory protein FleQ | strong | 42 | 68 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_18660 | | AAA family ATPase | strong | 42 | 68 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_05045 | | transcriptional regulator FleQ | strong | 43 | 68 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_2822 | | Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains | insig. | 45 | 71 |
| | Pedobacter sp. GW460-11-11-14-LB5 | CA265_RS01040 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 44 | 66 |
| | Pantoea sp. MT58 | IAI47_19790 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 37 | 94 |
| | Pseudomonas simiae WCS417 | PS417_11085 | | ATPase AAA | insig. | 37 | 96 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_1790 | | Sigma-54 dependent transcriptional regulator | insig. | 37 | 96 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_4357 | | Flagellar regulatory protein FleQ | insig. | 43 | 68 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS26850 | | ATPase AAA | strong | 38 | 96 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS24790 | | sigma-54 dependent transcriptional regulator | cofit | 41 | 73 |
| | Paraburkholderia graminis OAS925 | ABIE53_001365 | | DNA-binding NtrC family response regulator | strong | 39 | 80 |
| | Pseudomonas segetis P6 | MPMX49_03150 | | Regulatory protein AtoC | strong | 43 | 67 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2114 | atoC | sigma-54 dependent transcriptional regulator/response regulator (TIGR) | insig. | 43 | 73 |
| | Lysobacter sp. OAE881 | ABIE51_RS01360 | | nitrogen regulation protein NR(I) | cofit | 39 | 91 |
| | Pseudomonas putida KT2440 | PP_2790 | | Sigma-54 dependent sensory box protein | strong | 36 | 96 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS15090 | | sigma-54-dependent Fis family transcriptional regulator | insig. | 42 | 72 |