| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Azospirillum sp. SherDot2 | MPMX19_02815 | | 2-keto-4-pentenoate hydratase | insig. | 100 | 100 |
| | Azospirillum sp. SherDot2 | MPMX19_05874 | | 2-keto-4-pentenoate hydratase | insig. | 59 | 100 |
| | Azospirillum brasilense Sp245 | AZOBR_RS15705 | | 2-keto-4-pentenoate hydratase | insig. | 57 | 99 |
| o | Acidovorax sp. GW101-3H11 | Ac3H11_833 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 49 | 92 |
| o | Paraburkholderia graminis OAS925 | ABIE53_002319 | | 2-keto-4-pentenoate hydratase | insig. | 41 | 94 |
| o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_05231 | | 2-keto-4-pentenoate hydratase | sig. | 40 | 94 |
| o | Paraburkholderia sabiae LMG 24235 | QEN71_RS07370 | | hydratase | insig. | 37 | 94 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS17015 | | 2-keto-4-pentenoate hydratase | insig. | 36 | 93 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS11195 | | hydratase | insig. | 39 | 95 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_03564 | | 2-keto-4-pentenoate hydratase | insig. | 34 | 93 |
| o | Herbaspirillum seropedicae SmR1 | HSERO_RS13395 | | hydratase | insig. | 32 | 96 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS34430 | | 2-keto-4-pentenoate hydratase | sig. | 35 | 93 |
| | Paraburkholderia graminis OAS925 | ABIE53_004692 | | 2-keto-4-pentenoate hydratase | insig. | 35 | 94 |
| | Herbaspirillum seropedicae SmR1 | HSERO_RS16775 | | hydratase | insig. | 34 | 94 |
| o | Pseudomonas segetis P6 | ACVTMO_RS02135 | | fumarylacetoacetate hydrolase family protein | insig. | 33 | 85 |
| | Xanthobacter sp. DMC5 | GFF4544 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 34 | 99 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF2112 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 34 | 90 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_05455 | | 2-keto-4-pentenoate hydratase | insig. | 29 | 100 |
| | Variovorax sp. OAS795 | ABID97_RS15830 | | fumarylacetoacetate hydrolase family protein | insig. | 29 | 95 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2976 | | 2-keto-4-pentenoate hydratase | insig. | 30 | 93 |
| | Caulobacter crescentus NA1000 | CCNA_01577 | | 2-keto-4-pentenoate hydratase family protein | sig. | 27 | 93 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_01577 | | 2-keto-4-pentenoate hydratase family protein | cofit | 27 | 93 |
| | Mycobacterium tuberculosis H37Rv | Rv3536c | | Probable hydratase | insig. | 33 | 96 |
| | Bosea sp. OAE506 | ABIE41_RS20000 | | hypothetical protein | strong | 32 | 94 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS28745 | | 2-keto-4-pentenoate hydratase | insig. | 29 | 96 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS07645 | | fumarylacetoacetate hydrolase family protein | insig. | 27 | 88 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_27440 | | 2-keto-4-pentenoate hydratase | insig. | 32 | 81 |
| | Sphingobium sp. HT1-2 | GFF1734 | | hypothetical protein | insig. | 30 | 86 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_864 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 30 | 81 |
| | Escherichia coli ECOR38 | HEPCGN_07395 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 29 | 85 |
| | Escherichia coli ECRC62 | BNILDI_23095 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 28 | 85 |
| | Shewanella sp. ANA-3 | Shewana3_3602 | | hydratase/decarboxylase family protein (RefSeq) | insig. | 28 | 96 |
| | Escherichia coli ECOR27 | NOLOHH_01685 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 28 | 85 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_03321 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 85 |
| | Escherichia coli BL21 | ECD_00304 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 85 |
| | Escherichia coli BW25113 | b0350 | mhpD | 2-keto-4-pentenoate hydratase (VIMSS) | insig. | 28 | 85 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS27885 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 95 |
| | Escherichia coli ECRC100 | OKFHMN_08770 | mhpD | 2-keto-4-pentenoate hydratase | cofit | 29 | 82 |
| | Escherichia coli ECRC101 | MCAODC_28000 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 29 | 82 |
| | Escherichia coli ECRC102 | NIAGMN_06825 | mhpD | 2-keto-4-pentenoate hydratase | cofit | 29 | 82 |
| | Escherichia coli ECRC98 | JDDGAC_12425 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 29 | 82 |
| | Escherichia coli ECRC99 | KEDOAH_19200 | mhpD | 2-keto-4-pentenoate hydratase | no data | 29 | 82 |
| | Klebsiella michiganensis M5al | BWI76_RS16910 | | 2-keto-4-pentenoate hydratase | strong | 29 | 84 |
| | Shewanella oneidensis MR-1 | SO4042 | | hydratase/decarboxylase family protein (NCBI ptt file) | insig. | 27 | 96 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_24060 | | 2-keto-4-pentenoate hydratase | insig. | 29 | 83 |
| | Rhizobium sp. OAE497 | ABIE40_RS24835 | | fumarylacetoacetate hydrolase family protein | insig. | 34 | 72 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.200.1 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | sig. | 29 | 88 |
| | Variovorax sp. OAS795 | ABID97_RS27940 | | 2-keto-4-pentenoate hydratase | no data | 30 | 84 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS32645 | | 2-keto-4-pentenoate hydratase | insig. | 30 | 70 |
| | Rhizobium sp. OAE497 | ABIE40_RS22530 | | fumarylacetoacetate hydrolase family protein | insig. | 30 | 96 |
| | Acinetobacter radioresistens SK82 | MPMX26_00319 | | 2-keto-4-pentenoate hydratase | insig. | 25 | 97 |
| | Lysobacter sp. OAE881 | ABIE51_RS02855 | | 2-keto-4-pentenoate hydratase | insig. | 30 | 93 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c32190 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase HpcG | insig. | 24 | 99 |
| | Pseudomonas putida KT2440 | PP_2652 | | putative Hydratase/decarboxylase | insig. | 29 | 92 |
| | Xanthobacter sp. DMC5 | GFF2802 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 27 | 98 |
| | Marinobacter adhaerens HP15 | HP15_4034 | | hydratase/decarboxylase | insig. | 27 | 79 |
| | Serratia liquefaciens MT49 | IAI46_05780 | | fumarylacetoacetate hydrolase family protein | insig. | 25 | 93 |
| | Pseudomonas sp. SVBP6 | COO64_RS13765 | hpaH | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 27 | 96 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS28115 | hpaH;go_function=2-oxo-hept-3-ene-1%2C7-dioate | 2-oxo-hepta-3-ene-1 7-dioic acid hydratase | insig. | 27 | 96 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_21255 | | 2-oxo-hepta-3-ene-1 7-dioic acid hydratase | insig. | 27 | 96 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS47525 | hpaH;go_function=2-oxo-hept-3-ene-1%2C7-dioate | 2-oxo-hepta-3-ene-1 7-dioic acid hydratase | sig. | 27 | 96 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS31850 | | 2-keto-4-pentenoate hydratase | insig. | 29 | 76 |
| o | Agrobacterium fabrum C58 | Atu4592 | | hypothetical protein | sig. | 30 | 85 |
| | Pseudomonas sp. BP01 | JOY50_RS02805 | | 2-keto-4-pentenoate hydratase | insig. | 29 | 91 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_25565 | | 2-oxo-hepta-3-ene-1,7-dioate hydratase | insig. | 27 | 80 |
| | Marinobacter adhaerens HP15 | HP15_1113 | | hydratase/decarboxylase | insig. | 27 | 79 |
| | Sphingobium sp. HT1-2 | GFF555 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | cofit | 27 | 98 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS10275 | | 2-oxopent-4-enoate hydratase | insig. | 26 | 94 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_1313 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 27 | 80 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_02382 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | cofit | 27 | 80 |
| | Pseudomonas simiae WCS417 | PS417_17860 | | 2-oxo-hepta-3-ene-1,7-dioate hydratase | insig. | 27 | 78 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_0239 | | 2-keto-4-pentenoate hydratase | insig. | 24 | 86 |
| | Marinobacter adhaerens HP15 | HP15_1116 | | hydratase/decarboxylase | insig. | 29 | 61 |
| | Marinobacter adhaerens HP15 | HP15_4037 | | hydratase/decarboxylase | insig. | 29 | 61 |
| | Pseudomonas orientalis W4I3 | QF045_RS12905 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 27 | 95 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS15950 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 27 | 80 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS15950 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | strong | 27 | 80 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS27850 | | 2-oxo-hepta-3-ene-1,7-dioate hydratase | insig. | 27 | 83 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01664 | | 2-oxo-3-hexenedioate decarboxylase | insig. | 33 | 63 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS07240 | | 2-keto-4-pentenoate hydratase | no data | 30 | 63 |
| | Ralstonia solanacearum GMI1000 | RS_RS21400 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 80 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS28085 | | fumarylacetoacetate hydrolase family protein | insig. | 35 | 33 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_21225 | | 4-hydroxyphenylacetate isomerase | sig. | 35 | 33 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS47555 | | fumarylacetoacetate hydrolase family protein | insig. | 35 | 33 |
| | Alteromonas macleodii MIT1002 | MIT1002_02097 | | 2-hydroxypent-2,4-dienoate hydratase | insig. | 26 | 71 |
| | Variovorax sp. SCN45 | GFF7098 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 30 | 65 |
| | Azospirillum sp. SherDot2 | MPMX19_06121 | | hypothetical protein | insig. | 30 | 96 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2504 | | 4-oxalocrotonate decarboxylase (EC 4.1.1.77) | sig. | 29 | 60 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS13510 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 26 | 83 |
| | Pseudomonas sp. BP01 | JOY50_RS14050 | | fumarylacetoacetate hydrolase family protein | no data | 26 | 62 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01663 | | 2-keto-4-pentenoate hydratase | no data | 24 | 72 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_1307 | | 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-) | insig. | 34 | 31 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS28900 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 26 | 83 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_25595 | | 4-hydroxyphenylacetate isomerase | insig. | 34 | 33 |
| | Ralstonia solanacearum GMI1000 | RS_RS21405 | | 4-oxalocrotonate decarboxylase | insig. | 29 | 62 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS34400 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | no data | 27 | 83 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS12050 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 26 | 83 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS09290 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 26 | 99 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1824 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 26 | 67 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS10280 | | 2-oxo-3-hexenedioate decarboxylase | insig. | 29 | 62 |