| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Methanococcus maripaludis S2 | MMP_RS06510 | | Glu-tRNA(Gln) amidotransferase subunit GatD | no data | 100 | 100 |
| o | Methanococcus maripaludis JJ | MMJJ_RS07840 | | Glu-tRNA(Gln) amidotransferase subunit GatD | sig. | 98 | 100 |
| | Rahnella sp. WP5 | EX31_RS06300 | | asparaginase | insig. | 33 | 68 |
| | Pectobacterium carotovorum WPP14 | HER17_RS11110 | | asparaginase | insig. | 32 | 69 |
| | Dickeya dadantii 3937 | DDA3937_RS10285 | | asparaginase | insig. | 31 | 69 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_1792 | | L-asparaginases, type I | insig. | 32 | 68 |
| | Dickeya dianthicola 67-19 | HGI48_RS10295 | | asparaginase | insig. | 31 | 69 |
| | Dickeya dianthicola ME23 | DZA65_RS10770 | | asparaginase | insig. | 31 | 69 |
| o | Shewanella loihica PV-4 | Shew_1940 | ansA | cytoplasmic asparaginase I (RefSeq) | no data | 31 | 82 |
| | Enterobacter sp. TBS_079 | MPMX20_01849 | | L-asparaginase 1 | strong | 31 | 68 |
| | Escherichia coli BL21 | ECD_01736 | | cytoplasmic L-asparaginase 1 | insig. | 31 | 68 |
| | Escherichia coli ECOR27 | NOLOHH_17320 | ansA | asparaginase | insig. | 31 | 68 |
| | Escherichia coli ECOR38 | HEPCGN_05180 | ansA | asparaginase | insig. | 31 | 68 |
| | Escherichia coli ECRC100 | OKFHMN_26805 | ansA | asparaginase | insig. | 31 | 68 |
| | Escherichia coli ECRC101 | MCAODC_18605 | ansA | asparaginase | insig. | 31 | 68 |
| | Escherichia coli ECRC102 | NIAGMN_01260 | ansA | asparaginase | insig. | 31 | 68 |
| | Escherichia coli ECRC62 | BNILDI_16105 | ansA | asparaginase | cofit | 31 | 68 |
| | Escherichia coli ECRC98 | JDDGAC_01380 | ansA | asparaginase | insig. | 31 | 68 |
| | Escherichia coli ECRC99 | KEDOAH_01305 | ansA | asparaginase | no data | 31 | 68 |
| | Escherichia coli BW25113 | b1767 | ansA | cytoplasmic asparaginase I (NCBI) | strong | 31 | 68 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01965 | | L-asparaginase 1 | insig. | 31 | 68 |
| | Enterobacter asburiae PDN3 | EX28DRAFT_1768 | | L-asparaginase, type I | cofit | 30 | 68 |
| o | Pseudomonas sp. DMC3 | GFF490 | | L-asparaginase 1 | cofit | 30 | 82 |
| o | Alteromonas macleodii MIT1002 | MIT1002_01844 | | L-asparaginase 1 | insig. | 31 | 81 |
| o | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_03920 | | L-asparaginase 1 | insig. | 31 | 81 |
| | Cellulophaga baltica 18 | M666_RS07465 | | asparaginase | insig. | 32 | 76 |
| | Erwinia amylovora T8 | OLJFJH_13240 | | asparaginase | strong | 30 | 68 |
| | Escherichia fergusonii Becca | EFB2_02227 | | L-asparaginase 1 | insig. | 30 | 68 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS09860 | | asparaginase | no data | 30 | 68 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_740 | | L-asparaginase (EC 3.5.1.1) (from data) | strong | 29 | 83 |
| o | Bacteroides thetaiotaomicron VPI-5482 | BT0526 | | L-asparaginase I (NCBI ptt file) | strong | 31 | 81 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF3482 | | L-asparaginase I, cytoplasmic (EC 3.5.1.1) | insig. | 31 | 68 |
| | Klebsiella michiganensis M5al | BWI76_RS11580 | | L-asparaginase 1 | insig. | 30 | 68 |
| o | Bacteroides stercoris CC31F | HMPREF1181_RS00065 | | type I asparaginase | strong | 30 | 82 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_19250 | | asparaginase | sig. | 30 | 68 |
| o | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS02800 | | asparaginase | cofit | 28 | 81 |
| o | Pantoea sp. MT58 | IAI47_08965 | | asparaginase | strong | 29 | 81 |
| | Shewanella sp. ANA-3 | Shewana3_1963 | ansA | cytoplasmic asparaginase I (RefSeq) | sig. | 34 | 68 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_09960 | | L-asparaginase (EC 3.5.1.1) (from data) | strong | 28 | 82 |
| o | Bacteroides ovatus ATCC 8483 | BACOVA_03787 | | L-asparaginase, type I | cofit | 31 | 81 |
| | Bacteroides ovatus ATCC 8483 | BACOVA_03020 | | L-asparaginase, type I | insig. | 30 | 82 |
| o | Serratia liquefaciens MT49 | IAI46_14330 | | asparaginase | no data | 29 | 81 |
| o | Parabacteroides merdae CL09T00C40 | HMPREF1078_RS07865 | | asparaginase | insig. | 30 | 80 |
| o | Vibrio cholerae E7946 ATCC 55056 | CSW01_09955 | | L-asparaginase 1 | insig. | 32 | 81 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_00094 | | L-asparaginase 1 | cofit | 29 | 81 |
| o | Shewanella oneidensis MR-1 | SO2421 | ansA | L-asparaginase I (NCBI ptt file) | strong | 32 | 81 |
| o | Pseudomonas sp. RS175 | PFR28_04501 | | L-asparaginase 1 | sig. | 27 | 83 |
| o | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_0080 | | L-asparaginases, type I | strong | 30 | 84 |
| o | Mucilaginibacter yixingensis YX-36 DSM 26809 | ABZR88_RS21610 | | asparaginase | strong | 30 | 82 |
| | Erwinia tracheiphila SCR3 | LU632_RS12975 | ansA | asparaginase | insig. | 30 | 68 |
| | Shewanella amazonensis SB2B | Sama_1722 | ansA | cytoplasmic asparaginase I (RefSeq) | strong | 33 | 68 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_22635 | ansA | asparaginase | cofit | 29 | 68 |
| | Pseudomonas segetis P6 | MPMX49_00165 | | L-asparaginase 1 | cofit | 29 | 79 |
| o | Pseudomonas orientalis W4I3 | QF045_RS02500 | | asparaginase | cofit | 28 | 81 |
| o | Pseudomonas stutzeri RCH2 | Psest_4078 | | L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D | strong | 27 | 82 |
| o | Pseudomonas simiae WCS417 | PS417_28140 | | asparaginase | strong | 27 | 81 |
| | Bacteroides thetaiotaomicron VPI-5482 | BT2404 | | L-asparaginase I (NCBI ptt file) | insig. | 31 | 76 |
| o | Pedobacter sp. GW460-11-11-14-LB5 | CA265_RS25090 | | L-asparaginase (EC 3.5.1.1) (from data) | sig. | 29 | 81 |
| | Phocaeicola dorei CL03T12C01 | ABI39_RS14325 | | L-asparaginase 2 | strong | 30 | 76 |
| o | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_2023 | | L-asparaginase (EC 3.5.1.1) (from data) | sig. | 28 | 83 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_4087 | | L-asparaginase (EC 3.5.1.1) (from data) | strong | 27 | 83 |
| | Bacteroides thetaiotaomicron VPI-5482 | BT2757 | | L-asparaginase II precursor (NCBI ptt file) | sig. | 33 | 68 |
| | Bacteroides stercoris CC31F | HMPREF1181_RS15355 | | type I asparaginase | insig. | 30 | 81 |
| | Parabacteroides merdae CL09T00C40 | HMPREF1078_RS19195 | | type I asparaginase | insig. | 29 | 80 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_11055 | | asparaginase | strong | 29 | 81 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS29825 | | asparaginase | insig. | 27 | 81 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS29825 | | asparaginase | strong | 27 | 81 |
| | Escherichia coli ECOR27 | NOLOHH_11080 | ansB | L-asparaginase 2 | insig. | 34 | 67 |
| | Bacteroides ovatus ATCC 8483 | BACOVA_01071 | | L-asparaginase, type II | strong | 32 | 68 |
| | Phocaeicola vulgatus CL09T03C04 | HMPREF1058_RS18010 | | L-asparaginase 2 | cofit | 30 | 76 |
| o | Pseudomonas sp. S08-1 | OH686_13275 | | L-asparaginase I, cytoplasmic | cofit | 28 | 82 |
| | Escherichia coli BL21 | ECD_02787 | | periplasmic L-asparaginase 2 | cofit | 34 | 67 |
| | Escherichia fergusonii Becca | EFB2_00945 | | L-asparaginase 2 | insig. | 34 | 67 |
| | Escherichia coli ECOR38 | HEPCGN_18490 | ansB | L-asparaginase 2 | insig. | 34 | 67 |
| | Escherichia coli Nissle 1917 | ECOLIN_RS16445 | | L-asparaginase 2 | insig. | 34 | 67 |
| o | Pseudomonas aeruginosa MRSN321 | DY961_RS17645 | | asparaginase | sig. | 29 | 81 |
| o | Pseudomonas aeruginosa PUPa3 | DQ20_RS39875 | | asparaginase | strong | 29 | 81 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_11100 | | L-asparaginase (EC 3.5.1.1) (from data) | strong | 27 | 83 |
| | Phocaeicola dorei CL03T12C01 | ABI39_RS21080 | | type I asparaginase | strong | 31 | 81 |
| | Escherichia coli ECRC100 | OKFHMN_19220 | ansB | L-asparaginase 2 | cofit | 33 | 67 |
| | Escherichia coli ECRC101 | MCAODC_09740 | ansB | L-asparaginase 2 | insig. | 33 | 67 |
| | Escherichia coli ECRC102 | NIAGMN_16985 | ansB | L-asparaginase 2 | cofit | 33 | 67 |
| | Escherichia coli ECRC98 | JDDGAC_22850 | ansB | L-asparaginase 2 | insig. | 33 | 67 |
| | Escherichia coli ECRC99 | KEDOAH_08920 | ansB | L-asparaginase 2 | no data | 33 | 67 |
| | Phocaeicola vulgatus CL09T03C04 | HMPREF1058_RS07205 | | type I asparaginase | strong | 31 | 81 |
| | Escherichia coli ECRC62 | BNILDI_09665 | ansB | L-asparaginase 2 | cofit | 33 | 67 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_00759 | | L-asparaginase 2 | insig. | 33 | 67 |
| | Fusobacterium nucleatum SB010 | HUW76_04600 | | asparaginase | cofit | 28 | 79 |
| o | Pseudomonas sp. SVBP6 | COO64_RS15980 | | asparaginase | insig. | 28 | 81 |
| | Escherichia coli BW25113 | b2957 | ansB | periplasmic L-asparaginase II (NCBI) | insig. | 32 | 67 |
| | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF3060 | | L-asparaginase (EC 3.5.1.1) | insig. | 33 | 67 |
| | Pseudomonas sp. SVBP6 | COO64_RS01770 | | asparaginase | insig. | 27 | 81 |
| | Bacteroides stercoris CC31F | HMPREF1181_RS11260 | | L-asparaginase 2 | sig. | 30 | 75 |
| o | Pseudomonas putida KT2440 | PP_0495 | | type 1 L-asparaginase | strong | 27 | 81 |
| | Serratia liquefaciens MT49 | IAI46_08625 | | L-asparaginase 2 | insig. | 30 | 64 |
| | Azospirillum sp. SherDot2 | MPMX19_02859 | | L-asparaginase 2 | insig. | 31 | 70 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS33800 | | asparaginase | insig. | 29 | 69 |
| o | Pseudomonas syringae pv. syringae B728a | Psyr_5053 | | asparaginase | sig. | 25 | 81 |
| o | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_5053 | | asparaginase | cofit | 25 | 81 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_15190 | | asparaginase | insig. | 27 | 69 |