Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| Marinobacter adhaerens HP15 | HP15_4034 | | hydratase/decarboxylase | insig. | 100 | 100 |
| Marinobacter adhaerens HP15 | HP15_1113 | | hydratase/decarboxylase | insig. | 96 | 100 |
o | Alteromonas macleodii MIT1002 | MIT1002_02097 | | 2-hydroxypent-2,4-dienoate hydratase | insig. | 77 | 100 |
o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2504 | | 4-oxalocrotonate decarboxylase (EC 4.1.1.77) | sig. | 64 | 97 |
o | Cupriavidus basilensis FW507-4G11 | RR42_RS32645 | | 2-keto-4-pentenoate hydratase | insig. | 62 | 100 |
| Cupriavidus basilensis FW507-4G11 | RR42_RS31850 | | 2-keto-4-pentenoate hydratase | insig. | 59 | 100 |
o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01663 | | 2-keto-4-pentenoate hydratase | no data | 49 | 93 |
o | Burkholderia phytofirmans PsJN | BPHYT_RS07240 | | 2-keto-4-pentenoate hydratase | no data | 44 | 100 |
o | Acinetobacter radioresistens SK82 | MPMX26_00319 | | 2-keto-4-pentenoate hydratase | insig. | 42 | 98 |
o | Serratia liquefaciens MT49 | IAI46_05780 | | fumarylacetoacetate hydrolase family protein | insig. | 47 | 95 |
o | Variovorax sp. OAS795 | ABID97_RS27940 | | 2-keto-4-pentenoate hydratase | no data | 46 | 91 |
o | Escherichia coli ECOR38 | HEPCGN_07395 | mhpD | 2-keto-4-pentenoate hydratase | cofit | 41 | 100 |
o | Mycobacterium tuberculosis H37Rv | Rv3536c | | Probable hydratase | insig. | 42 | 97 |
o | Escherichia coli BW25113 | b0350 | mhpD | 2-keto-4-pentenoate hydratase (VIMSS) | insig. | 41 | 100 |
o | Escherichia coli BL21 | ECD_00304 | | 2-keto-4-pentenoate hydratase | insig. | 41 | 100 |
o | Escherichia coli ECOR27 | NOLOHH_01685 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 41 | 100 |
o | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_03321 | | 2-keto-4-pentenoate hydratase | insig. | 41 | 100 |
| Cupriavidus basilensis FW507-4G11 | RR42_RS05105 | | 2-keto-4-pentenoate hydratase | no data | 42 | 98 |
o | Escherichia coli ECRC62 | BNILDI_23095 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 41 | 100 |
o | Escherichia coli ECRC101 | OKFHMN_08770 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 41 | 97 |
o | Escherichia coli ECRC101 | MCAODC_28000 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 41 | 97 |
o | Escherichia coli ECRC102 | NIAGMN_06825 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 41 | 97 |
o | Escherichia coli ECRC98 | JDDGAC_12425 | mhpD | 2-keto-4-pentenoate hydratase | no data | 41 | 97 |
o | Escherichia coli ECRC99 | KEDOAH_19200 | mhpD | 2-keto-4-pentenoate hydratase | no data | 41 | 97 |
| Ralstonia solanacearum GMI1000 | RS_RS21400 | | 2-keto-4-pentenoate hydratase | insig. | 43 | 93 |
o | Klebsiella michiganensis M5al | BWI76_RS16910 | | 2-keto-4-pentenoate hydratase | strong | 38 | 100 |
o | Pseudomonas fluorescens SBW25 | PFLU_RS15950 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 41 | 100 |
o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS15950 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | strong | 41 | 100 |
| Pseudomonas fluorescens FW300-N2C3 | AO356_25565 | | 2-oxo-hepta-3-ene-1,7-dioate hydratase | insig. | 40 | 100 |
| Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_1313 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 39 | 100 |
| Variovorax sp. OAS795 | ABID97_RS03725 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | strong | 41 | 98 |
o | Variovorax sp. SCN45 | GFF5098 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 40 | 100 |
o | Acidovorax sp. GW101-3H11 | Ac3H11_1483 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 41 | 93 |
| Serratia liquefaciens MT49 | IAI46_02750 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 40 | 95 |
| Escherichia coli BL21 | ECD_04226 | | 2-oxo-hept-3-ene-1,7-dioate hydratase | insig. | 38 | 97 |
| Escherichia coli ECOR27 | NOLOHH_03735 | hpaH | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 38 | 97 |
| Escherichia coli ECRC62 | BNILDI_07865 | hpaH | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 38 | 97 |
| Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_03714 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 38 | 97 |
| Acidovorax sp. GW101-3H11 | Ac3H11_4809 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 40 | 100 |
o | Enterobacter asburiae PDN3 | EX28DRAFT_3543 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 38 | 97 |
o | Enterobacter sp. TBS_079 | MPMX20_00638 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | sig. | 38 | 97 |
o | Pseudomonas simiae WCS417 | PS417_17860 | | 2-oxo-hepta-3-ene-1,7-dioate hydratase | insig. | 40 | 100 |
| Klebsiella michiganensis M5al | BWI76_RS03850 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | sig. | 38 | 97 |
| Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF3282 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | cofit | 38 | 97 |
| Paraburkholderia sabiae LMG 24235 | QEN71_RS12050 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 39 | 100 |
| Cupriavidus basilensis FW507-4G11 | RR42_RS27885 | | 2-keto-4-pentenoate hydratase | insig. | 43 | 97 |
| Alteromonas macleodii MIT1002 | MIT1002_02100 | | 2-hydroxyhexa-2,4-dienoate hydratase | insig. | 39 | 100 |
| Cupriavidus basilensis FW507-4G11 | RR42_RS27850 | | 2-oxo-hepta-3-ene-1,7-dioate hydratase | insig. | 39 | 98 |
| Variovorax sp. SCN45 | GFF7098 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 40 | 98 |
| Azospirillum sp. SherDot2 | MPMX19_06796 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 39 | 95 |
o | Rhizobium sp. OAE497 | ABIE40_RS20915 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 39 | 100 |
| Paraburkholderia graminis OAS925 | ABIE53_005224 | | 2-oxo-hept-3-ene-1,7-dioate hydratase | insig. | 38 | 100 |
| Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_03253 | | 2-oxohept-3-enedioate hydratase | insig. | 38 | 100 |
| Ralstonia sp. UNC404CL21Col | ABZR87_RS13510 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 37 | 100 |
| Marinobacter adhaerens HP15 | HP15_1116 | | hydratase/decarboxylase | insig. | 38 | 100 |
| Marinobacter adhaerens HP15 | HP15_4037 | | hydratase/decarboxylase | insig. | 38 | 100 |
| Rhodopseudomonas palustris CGA009 | TX73_019460 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 38 | 100 |
o | Castellaniella sp019104865 MT123 | ABCV34_RS09290 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 39 | 95 |
o | Magnetospirillum magneticum AMB-1 | AMB_RS04100 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 36 | 98 |
| Burkholderia phytofirmans PsJN | BPHYT_RS28900 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 38 | 100 |
| Paraburkholderia sabiae LMG 24235 | QEN71_RS34400 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | no data | 38 | 100 |
o | Phaeobacter inhibens DSM 17395 | PGA1_c32190 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase HpcG | insig. | 38 | 99 |
| Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1824 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 37 | 100 |
| Cupriavidus basilensis FW507-4G11 | RR42_RS32640 | | 4-oxalocrotonate decarboxylase | no data | 40 | 100 |
| Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_05455 | | 2-keto-4-pentenoate hydratase | insig. | 41 | 90 |
| Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01664 | | 2-oxo-3-hexenedioate decarboxylase | insig. | 38 | 89 |
| Ralstonia solanacearum GMI1000 | RS_RS21405 | | 4-oxalocrotonate decarboxylase | insig. | 35 | 86 |
| Burkholderia phytofirmans PsJN | BPHYT_RS07255 | | 4-oxalocrotonate decarboxylase | strong | 33 | 93 |
| Serratia liquefaciens MT49 | IAI46_05765 | | fumarylacetoacetate hydrolase family protein | insig. | 34 | 97 |
| Variovorax sp. OAS795 | ABID97_RS27935 | | fumarylacetoacetate hydrolase family protein | no data | 36 | 97 |
| Pseudomonas fluorescens FW300-N2C3 | AO356_28650 | | 2-keto-4-pentenoate hydratase | insig. | 33 | 85 |
| Pseudomonas fluorescens SBW25 | PFLU_RS15025 | | 2-keto-4-pentenoate hydratase | insig. | 32 | 87 |
| Pseudomonas fluorescens SBW25-INTG | PFLU_RS15025 | | 2-keto-4-pentenoate hydratase | insig. | 32 | 87 |
o | Herbaspirillum seropedicae SmR1 | HSERO_RS13395 | | hydratase | insig. | 31 | 100 |
| Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF2112 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 28 | 84 |
| Rhizobium sp. OAE497 | ABIE40_RS22530 | | fumarylacetoacetate hydrolase family protein | insig. | 34 | 85 |
| Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF126 | | Hydratase/decarboxylase | no data | 28 | 85 |
o | Kangiella aquimarina DSM 16071 | B158DRAFT_0239 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 82 |
| Variovorax sp. SCN45 | GFF3428 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 26 | 83 |
| Acidovorax sp. GW101-3H11 | Ac3H11_2496 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 29 | 82 |
o | Azospirillum brasilense Sp245 | AZOBR_RS15705 | | 2-keto-4-pentenoate hydratase | insig. | 27 | 94 |
| Dinoroseobacter shibae DFL-12 | Dshi_0880 | | Hydratase/decarboxylase (RefSeq) | insig. | 27 | 84 |
| Azospirillum sp. SherDot2 | MPMX19_02815 | | 2-keto-4-pentenoate hydratase | insig. | 27 | 76 |
| Herbaspirillum seropedicae SmR1 | HSERO_RS02100 | | 4-oxalocrotonate decarboxylase | sig. | 24 | 90 |
| Azospirillum sp. SherDot2 | MPMX19_05874 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 59 |
| Paraburkholderia graminis OAS925 | ABIE53_002319 | | 2-keto-4-pentenoate hydratase | insig. | 29 | 59 |
| Burkholderia phytofirmans PsJN | BPHYT_RS11195 | | hydratase | insig. | 28 | 59 |
| Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_05231 | | 2-keto-4-pentenoate hydratase | sig. | 29 | 59 |
| Paraburkholderia sabiae LMG 24235 | QEN71_RS07370 | | hydratase | insig. | 28 | 59 |
| Burkholderia phytofirmans PsJN | BPHYT_RS34430 | | 2-keto-4-pentenoate hydratase | sig. | 27 | 69 |
| Pseudomonas fluorescens FW300-N2C3 | AO356_27440 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 61 |
| Acidovorax sp. GW101-3H11 | Ac3H11_833 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 31 | 59 |
| Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_864 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 28 | 61 |
| Shewanella sp. ANA-3 | Shewana3_3602 | | hydratase/decarboxylase family protein (RefSeq) | insig. | 24 | 98 |
| Burkholderia phytofirmans PsJN | BPHYT_RS28745 | | 2-keto-4-pentenoate hydratase | insig. | 27 | 60 |
| Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_03564 | | 2-keto-4-pentenoate hydratase | insig. | 27 | 79 |
| Variovorax sp. OAS795 | ABID97_RS15830 | | fumarylacetoacetate hydrolase family protein | insig. | 29 | 61 |
| Shewanella oneidensis MR-1 | SO4042 | | hydratase/decarboxylase family protein (NCBI ptt file) | insig. | 23 | 98 |
| Sphingomonas koreensis DSMZ 15582 | Ga0059261_2976 | | 2-keto-4-pentenoate hydratase | insig. | 24 | 84 |