| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Variovorax sp. SCN45 | GFF4178 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | insig. | 100 | 100 |
| o | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.399.1 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | insig. | 75 | 95 |
| o | Rhizobium sp. OAE497 | ABIE40_RS20900 | | methylenetetrahydrofolate reductase | insig. | 37 | 93 |
| | Agrobacterium fabrum C58 | Atu1764 | | methylenetetrahydrofolate reductase | sig. | 39 | 78 |
| o | Sinorhizobium meliloti 1021 | SMa2143 | | 5,10-methylenetetrahydrofolate reductase | insig. | 29 | 93 |
| o | Phaeobacter inhibens DSM 17395 | PGA1_c16050 | | 5,10-methylenetetrahydrofolate reductase | strong | 29 | 92 |
| o | Dinoroseobacter shibae DFL-12 | Dshi_1675 | | MetF protein-like protein (RefSeq) | strong | 30 | 96 |
| | Agrobacterium fabrum C58 | Atu1418 | | methylenetetrahydrofolate reductase | sig. | 28 | 97 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU0997 | metF | 5,10-methylenetetrahydrofolate reductase (TIGR) | strong | 31 | 74 |
| | Xanthobacter sp. DMC5 | GFF4147 | | 5,10-methylenetetrahydrofolate reductase | no data | 29 | 78 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS23495 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 28 | 83 |
| | Ralstonia solanacearum GMI1000 | RS_RS00460 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 28 | 83 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS04865 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 30 | 64 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS13550 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 28 | 69 |
| | Dechlorosoma suillum PS | Dsui_0640 | | 5,10-methylenetetrahydrofolate reductase | no data | 28 | 68 |
| | Bosea sp. OAE506 | ABIE41_RS12475 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 65 |
| | Rhodopseudomonas palustris CGA009 | TX73_019175 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 61 |
| | Sphingobium sp. HT1-2 | GFF217 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | strong | 29 | 71 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS09875 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 27 | 83 |
| | Ralstonia solanacearum UW163 | UW163_RS03705 | | methylenetetrahydrofolate reductase [NAD(P)H] | insig. | 27 | 83 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS01385 | | 5,10-methylenetetrahydrofolate reductase | strong | 28 | 64 |
| | Agrobacterium fabrum C58 | Atu2123 | | methylenetetrahydrofolate reductase | strong | 31 | 45 |
| | Pseudomonas sp. S08-1 | OH686_10820 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 28 | 60 |
| | Rhizobium sp. OAE497 | ABIE40_RS11950 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 28 | 66 |
| | Lysobacter sp. OAE881 | ABIE51_RS16490 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 30 | 65 |
| | Pseudomonas stutzeri RCH2 | Psest_0313 | | 5,10-methylenetetrahydrofolate reductase, prokaryotic form | strong | 28 | 52 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_05801 | | 5,10-methylenetetrahydrofolate reductase (NAD(P)) | strong | 26 | 67 |
| | Paraburkholderia graminis OAS925 | ABIE53_000061 | | methylenetetrahydrofolate reductase (NADPH) | strong | 26 | 67 |
| | Pseudomonas sp. DMC3 | GFF171 | | 5,10-methylenetetrahydrofolate reductase | strong | 29 | 44 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_3034 | | 5,10-methylenetetrahydrofolate reductase (RefSeq) | no data | 26 | 78 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_23270 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 52 |
| | Rhodospirillum rubrum S1H | Rru_A1530 | | 5,10-methylenetetrahydrofolate reductase (NCBI) | strong | 27 | 65 |
| | Pseudomonas aeruginosa MRSN321 | DY961_RS22135 | metF;go_function=methylenetetrahydrofolate | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 52 |
| | Pseudomonas aeruginosa PUPa3 | DQ20_RS37170 | metF;go_function=methylenetetrahydrofolate | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 52 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0992 | | 5,10-methylenetetrahydrofolate reductase, prokaryotic form | strong | 26 | 69 |
| | Pseudomonas sp. SVBP6 | COO64_RS17485 | metF | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 26 | 69 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS18705 | | 5,10-methylenetetrahydrofolate reductase | insig. | 25 | 64 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_4638 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | strong | 29 | 52 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.4336.1 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | strong | 28 | 71 |
| | Pseudomonas putida KT2440 | PP_4977 | | 5,10-methylenetetrahydrofolate reductase | strong | 29 | 52 |
| | Marinobacter adhaerens HP15 | HP15_2917 | | 5,10-methylenetetrahydrofolate reductase-like protein | strong | 25 | 60 |
| | Methylophilus sp. DMC18 | GFF1492 | | 5,10-methylenetetrahydrofolate reductase | no data | 23 | 60 |
| | Azospirillum brasilense Sp245 | AZOBR_RS24245 | | 5 10-methylenetetrahydrofolate reductase | insig. | 28 | 53 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS02230 | | methylenetetrahydrofolate reductase | no data | 27 | 63 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1692 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | no data | 27 | 60 |