| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Enterobacter asburiae PDN3 | EX28DRAFT_3543 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 100 | 100 |
| o | Enterobacter sp. TBS_079 | MPMX20_00638 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | sig. | 96 | 100 |
| o | Escherichia coli BL21 | ECD_04226 | | 2-oxo-hept-3-ene-1,7-dioate hydratase | insig. | 94 | 100 |
| o | Escherichia coli ECOR27 | NOLOHH_03735 | hpaH | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 94 | 100 |
| o | Escherichia coli ECRC62 | BNILDI_07865 | hpaH | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 94 | 100 |
| o | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_03714 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 94 | 100 |
| o | Klebsiella michiganensis M5al | BWI76_RS03850 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | sig. | 93 | 100 |
| o | Klebsiella pneumoniae MKP103 | KDGMDA_12100 | | 2-oxo-hepta-3-ene-1 7-dioic acid hydratase | insig. | 93 | 100 |
| o | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF3282 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | cofit | 91 | 100 |
| o | Serratia liquefaciens MT49 | IAI46_02750 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 85 | 100 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS27850 | | 2-oxo-hepta-3-ene-1,7-dioate hydratase | insig. | 75 | 96 |
| o | Ralstonia sp. UNC404CL21Col | ABZR87_RS13510 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 73 | 100 |
| o | Pseudomonas fluorescens SBW25 | PFLU_RS15950 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 72 | 100 |
| o | Pseudomonas fluorescens SBW25-INTG | PFLU_RS15950 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | strong | 72 | 100 |
| o | Pseudomonas simiae WCS417 | PS417_17860 | | 2-oxo-hepta-3-ene-1,7-dioate hydratase | insig. | 72 | 100 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_21255 | | 2-oxo-hepta-3-ene-1 7-dioic acid hydratase | insig. | 72 | 100 |
| o | Pseudomonas lactucae CFBP13502 | GEMAOFIL_02382 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | cofit | 71 | 100 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_25565 | | 2-oxo-hepta-3-ene-1,7-dioate hydratase | insig. | 70 | 100 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_1313 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 70 | 100 |
| o | Pseudomonas sp. SVBP6 | COO64_RS13765 | hpaH | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 71 | 100 |
| o | Variovorax sp. OAS795 | ABID97_RS03725 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | strong | 69 | 100 |
| o | Magnetospirillum magneticum AMB-1 | AMB_RS04100 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 70 | 96 |
| o | Variovorax sp. SCN45 | GFF5098 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 68 | 100 |
| o | Azospirillum sp. SherDot2 | MPMX19_06796 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 69 | 100 |
| o | Paraburkholderia sabiae LMG 24235 | QEN71_RS12050 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 65 | 100 |
| o | Acidovorax sp. GW101-3H11 | Ac3H11_4809 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 64 | 100 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS09290 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 68 | 97 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS34400 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | no data | 64 | 100 |
| o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_03253 | | 2-oxohept-3-enedioate hydratase | insig. | 63 | 100 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_1483 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 67 | 93 |
| o | Paraburkholderia graminis OAS925 | ABIE53_005224 | | 2-oxo-hept-3-ene-1,7-dioate hydratase | insig. | 63 | 100 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS28900 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 63 | 100 |
| o | Rhodopseudomonas palustris CGA009 | TX73_019460 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 63 | 96 |
| | Variovorax sp. SCN45 | GFF7098 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 57 | 97 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1824 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 53 | 100 |
| o | Xanthobacter sp. DMC5 | GFF2802 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 49 | 100 |
| o | Rhizobium sp. OAE497 | ABIE40_RS20915 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 54 | 96 |
| o | Phaeobacter inhibens DSM 17395 | PGA1_c32190 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase HpcG | insig. | 50 | 97 |
| o | Mycobacterium tuberculosis H37Rv | Rv3536c | | Probable hydratase | insig. | 41 | 100 |
| o | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS10275 | | 2-oxopent-4-enoate hydratase | insig. | 38 | 95 |
| o | Alteromonas macleodii MIT1002 | MIT1002_02097 | | 2-hydroxypent-2,4-dienoate hydratase | insig. | 35 | 100 |
| o | Marinobacter adhaerens HP15 | HP15_4034 | | hydratase/decarboxylase | insig. | 38 | 96 |
| | Alteromonas macleodii MIT1002 | MIT1002_02100 | | 2-hydroxyhexa-2,4-dienoate hydratase | insig. | 38 | 100 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS31850 | | 2-keto-4-pentenoate hydratase | insig. | 38 | 96 |
| | Marinobacter adhaerens HP15 | HP15_1113 | | hydratase/decarboxylase | insig. | 37 | 96 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS32645 | | 2-keto-4-pentenoate hydratase | insig. | 40 | 97 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS32640 | | 4-oxalocrotonate decarboxylase | no data | 38 | 100 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS27885 | | 2-keto-4-pentenoate hydratase | insig. | 37 | 96 |
| | Variovorax sp. OAS795 | ABID97_RS27940 | | 2-keto-4-pentenoate hydratase | no data | 38 | 88 |
| | Marinobacter adhaerens HP15 | HP15_4037 | | hydratase/decarboxylase | insig. | 37 | 99 |
| | Marinobacter adhaerens HP15 | HP15_1116 | | hydratase/decarboxylase | insig. | 37 | 96 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS10280 | | 2-oxo-3-hexenedioate decarboxylase | insig. | 36 | 100 |
| o | Pseudomonas sp. BP01 | JOY50_RS14050 | | fumarylacetoacetate hydrolase family protein | no data | 34 | 100 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01663 | | 2-keto-4-pentenoate hydratase | no data | 36 | 88 |
| o | Acinetobacter radioresistens SK82 | MPMX26_00319 | | 2-keto-4-pentenoate hydratase | insig. | 36 | 96 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS07240 | | 2-keto-4-pentenoate hydratase | no data | 35 | 99 |
| o | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2504 | | 4-oxalocrotonate decarboxylase (EC 4.1.1.77) | sig. | 34 | 96 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_24060 | | 2-keto-4-pentenoate hydratase | insig. | 33 | 97 |
| o | Escherichia coli ECOR38 | HEPCGN_07395 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 33 | 99 |
| | Escherichia coli BL21 | ECD_00304 | | 2-keto-4-pentenoate hydratase | insig. | 33 | 99 |
| | Klebsiella michiganensis M5al | BWI76_RS16910 | | 2-keto-4-pentenoate hydratase | strong | 33 | 97 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS05105 | | 2-keto-4-pentenoate hydratase | no data | 38 | 85 |
| o | Escherichia coli BW25113 | b0350 | mhpD | 2-keto-4-pentenoate hydratase (VIMSS) | insig. | 33 | 99 |
| | Escherichia coli ECOR27 | NOLOHH_01685 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 33 | 99 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_03321 | | 2-keto-4-pentenoate hydratase | insig. | 33 | 99 |
| | Escherichia coli ECRC62 | BNILDI_23095 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 33 | 99 |
| o | Escherichia coli ECRC100 | OKFHMN_08770 | mhpD | 2-keto-4-pentenoate hydratase | cofit | 33 | 97 |
| o | Escherichia coli ECRC101 | MCAODC_28000 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 33 | 97 |
| o | Escherichia coli ECRC102 | NIAGMN_06825 | mhpD | 2-keto-4-pentenoate hydratase | cofit | 33 | 97 |
| o | Escherichia coli ECRC98 | JDDGAC_12425 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 33 | 97 |
| o | Escherichia coli ECRC99 | KEDOAH_19200 | mhpD | 2-keto-4-pentenoate hydratase | no data | 33 | 97 |
| o | Ralstonia solanacearum GMI1000 | RS_RS21400 | | 2-keto-4-pentenoate hydratase | insig. | 32 | 99 |
| | Serratia liquefaciens MT49 | IAI46_05780 | | fumarylacetoacetate hydrolase family protein | insig. | 38 | 87 |
| o | Sphingobium sp. HT1-2 | GFF555 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | cofit | 35 | 87 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_05455 | | 2-keto-4-pentenoate hydratase | insig. | 33 | 88 |
| | Ralstonia solanacearum GMI1000 | RS_RS21405 | | 4-oxalocrotonate decarboxylase | insig. | 30 | 87 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS07255 | | 4-oxalocrotonate decarboxylase | strong | 29 | 94 |
| | Xanthobacter sp. DMC5 | GFF4544 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 30 | 95 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_28650 | | 2-keto-4-pentenoate hydratase | insig. | 30 | 91 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01664 | | 2-oxo-3-hexenedioate decarboxylase | insig. | 29 | 86 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS15025 | | 2-keto-4-pentenoate hydratase | insig. | 29 | 87 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS15025 | | 2-keto-4-pentenoate hydratase | insig. | 29 | 87 |
| | Variovorax sp. OAS795 | ABID97_RS27935 | | fumarylacetoacetate hydrolase family protein | no data | 32 | 87 |
| | Serratia liquefaciens MT49 | IAI46_05765 | | fumarylacetoacetate hydrolase family protein | insig. | 26 | 86 |
| | Herbaspirillum seropedicae SmR1 | HSERO_RS13395 | | hydratase | insig. | 28 | 79 |
| o | Kangiella aquimarina DSM 16071 | B158DRAFT_0239 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 85 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_01991 | | 2-hydroxyhexa-2,4-dienoate hydratase | cofit | 27 | 90 |
| | Rhizobium sp. OAE497 | ABIE40_RS22530 | | fumarylacetoacetate hydrolase family protein | insig. | 30 | 85 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF2112 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 26 | 85 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF126 | | Hydratase/decarboxylase | no data | 27 | 77 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_2496 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 29 | 84 |
| | Dinoroseobacter shibae DFL-12 | Dshi_0880 | | Hydratase/decarboxylase (RefSeq) | insig. | 28 | 81 |
| | Variovorax sp. SCN45 | GFF3428 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 23 | 78 |
| o | Shewanella oneidensis MR-1 | SO4042 | | hydratase/decarboxylase family protein (NCBI ptt file) | insig. | 25 | 87 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_27440 | | 2-keto-4-pentenoate hydratase | insig. | 29 | 61 |
| | Azospirillum brasilense Sp245 | AZOBR_RS15705 | | 2-keto-4-pentenoate hydratase | insig. | 25 | 70 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_864 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 26 | 81 |
| | Shewanella sp. ANA-3 | Shewana3_3602 | | hydratase/decarboxylase family protein (RefSeq) | insig. | 23 | 86 |
| | Azospirillum sp. SherDot2 | MPMX19_05874 | | 2-keto-4-pentenoate hydratase | insig. | 23 | 76 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS28745 | | 2-keto-4-pentenoate hydratase | insig. | 26 | 96 |