| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Sinorhizobium meliloti 1021 | SMa1315 | | VirB4 type IV secretion protein | insig. | 100 | 100 |
| o | Agrobacterium fabrum C58 | Atu5165 | | type IV secretion protein AvhB4 | sig. | 87 | 95 |
| | Agrobacterium fabrum C58 | Atu6170 | | component of type IV secretion system | insig. | 40 | 98 |
| o | Paraburkholderia sabiae LMG 24235 | QEN71_RS06710 | | VirB4 family type IV secretion/conjugal transfer ATPase | insig. | 40 | 98 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS32185 | | type IV secretion protein VirB4 | insig. | 40 | 98 |
| o | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.2054.1 | | hypothetical protein | insig. | 39 | 95 |
| o | Caulobacter crescentus NA1000 | CCNA_02501 | | type IV secretory pathway, VirB4 ATPase | cofit | 41 | 98 |
| o | Caulobacter crescentus NA1000 Δfur | CCNA_02501 | | type IV secretory pathway, VirB4 ATPase | cofit | 41 | 98 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS00780 | | type IV secretion protein VirB4 | insig. | 39 | 98 |
| o | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11435 | | VirB4 family type IV secretion/conjugal transfer ATPase | insig. | 37 | 98 |
| o | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS01895 | | VirB4 family type IV secretion/conjugal transfer ATPase | insig. | 40 | 99 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS03365 | | VirB4 family type IV secretion/conjugal transfer ATPase | insig. | 36 | 99 |
| o | Escherichia coli ECOR38 | HEPCGN_00295 | | ATPase | insig. | 31 | 97 |
| o | Escherichia coli ECOR27 | NOLOHH_00250 | | conjugal transfer protein TraB | cofit | 32 | 97 |
| o | Dickeya dianthicola ME23 | DZA65_RS14385 | | ATPase | insig. | 31 | 97 |
| o | Dickeya dianthicola 67-19 | HGI48_RS07125 | | VirB3 family type IV secretion system protein | insig. | 31 | 98 |
| o | Dickeya dadantii 3937 | DDA3937_RS13845 | | VirB3 family type IV secretion system protein | insig. | 31 | 98 |
| | Escherichia coli ECOR27 | NOLOHH_00340 | | ATPase | insig. | 31 | 97 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS36315 | | CagE TrbE VirB component of type IV transporter system | insig. | 31 | 99 |
| o | Acinetobacter radioresistens SK82 | MPMX26_03191 | | Type IV secretion system protein virB4 | cofit | 28 | 95 |
| o | Phaeobacter inhibens DSM 17395 | PGA1_c22850 | | type IV secretion system protein VirB | insig. | 31 | 94 |
| o | Ralstonia solanacearum UW163 | UW163_RS23835 | | transporter | insig. | 30 | 95 |
| o | Dinoroseobacter shibae DFL-12 | Dshi_3976 | | CagE TrbE VirB component of type IV transporter system (RefSeq) | insig. | 30 | 98 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3641 | | CagE TrbE VirB component of type IV transporter system (RefSeq) | insig. | 29 | 97 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS40950 | | transporter | insig. | 27 | 95 |
| o | Rhodopseudomonas palustris CGA009 | TX73_011510 | | conjugal transfer protein TrbE | cofit | 26 | 91 |
| o | Sphingomonas koreensis DSMZ 15582 | Ga0059261_3075 | | Type IV secretory pathway, VirB4 components | insig. | 26 | 87 |
| o | Xanthobacter sp. DMC5 | GFF3635 | | Type IV secretion system protein virB4 | insig. | 26 | 95 |
| o | Sphingobium sp. HT1-2 | GFF191 | | Conjugative transfer protein TrbE | cofit | 27 | 98 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2915 | | Type IV secretory pathway, VirB4 components | insig. | 26 | 94 |
| | Xanthobacter sp. DMC5 | GFF2638 | | Type IV secretion system protein virB4 | insig. | 26 | 98 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2569 | | Type IV secretory pathway, VirB4 components | insig. | 26 | 97 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0386 | | Type IV secretory pathway, VirB4 components | insig. | 27 | 84 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS00170 | | conjugal transfer protein TrbE | insig. | 25 | 89 |
| | Dickeya dianthicola 67-19 | HGI48_RS03910 | | conjugal transfer protein TrbE | insig. | 25 | 91 |
| | Rhodopseudomonas palustris CGA009 | TX73_021355 | | conjugal transfer protein TrbE | insig. | 25 | 89 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF3450 | | Conjugative transfer protein TrbE | insig. | 25 | 95 |
| o | Shewanella sp. ANA-3 | Shewana3_1285 | | conjugal transfer ATPase TrbE (RefSeq) | cofit | 25 | 91 |
| o | Variovorax sp. SCN45 | GFF4417 | | Conjugative transfer protein TrbE | insig. | 24 | 91 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4438 | | Conjugative transfer protein TrbE | insig. | 24 | 94 |
| | Variovorax sp. SCN45 | GFF4846 | | Conjugative transfer protein TrbE | insig. | 25 | 82 |
| o | Pseudomonas aeruginosa PA14 | IKLFDK_12840 | | Conjugal transfer protein TrbE | no data | 24 | 91 |
| | Dickeya dianthicola ME23 | DZA65_RS07630 | | ATPase | insig. | 33 | 41 |
| | Variovorax sp. SCN45 | GFF6576 | | Conjugative transfer protein TrbE | insig. | 24 | 91 |
| | Dickeya dianthicola 67-19 | HGI48_RS08435 | | conjugal transfer protein TrbE | insig. | 24 | 94 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4179 | | Conjugative transfer protein TrbE | insig. | 24 | 91 |
| o | Ralstonia solanacearum GMI1000 | RS_RS12940 | | conjugal transfer protein TrbE | insig. | 24 | 91 |
| | Dickeya dadantii 3937 | DDA3937_RS04010 | | conjugal transfer protein TrbE | insig. | 24 | 93 |
| | Agrobacterium fabrum C58 | Atu6038 | | conjugal transfer protein | insig. | 24 | 98 |
| o | Marinobacter adhaerens HP15 | HP15_p42g49 | | type IV secretion/conjugal transfer ATPase, VirB4 family | cofit | 22 | 92 |
| | Dechlorosoma suillum PS | Dsui_2577 | | type IV secretory pathway, VirB4 component | cofit | 22 | 69 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS43740 | | type IV secretion system protein VirB4 | insig. | 23 | 89 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_06158 | | type IV secretion system protein VirB4 | insig. | 27 | 64 |
| | Dickeya dianthicola ME23 | DZA65_RS07630 | | ATPase | insig. | 26 | 36 |
| | Azospirillum brasilense Sp245 | AZOBR_RS33145 | | hypothetical protein | insig. | 24 | 78 |
| | Phocaeicola vulgatus CL09T03C04 | HMPREF1058_RS09885 | | TraG family conjugative transposon ATPase | insig. | 22 | 49 |
| | Parabacteroides merdae CL09T00C40 | HMPREF1078_RS09030 | | TraG family conjugative transposon ATPase | sig. | 22 | 49 |
| | Phocaeicola dorei CL03T12C01 | ABI39_RS08805 | | TraG family conjugative transposon ATPase | insig. | 21 | 52 |
| | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_1362 | | Bacteroides conjugation system ATPase, TraG family | insig. | 22 | 50 |
| | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_09970 | | conjugal transfer protein TraG | insig. | 22 | 50 |
| | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_4363 | | Bacteroides conjugation system ATPase, TraG family | insig. | 25 | 36 |
| | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_1947 | | Bacteroides conjugation system ATPase, TraG family | sig. | 22 | 48 |
| | Variovorax sp. SCN45 | GFF7485 | | ATPase required for both assembly of type IV secretion complex and secretion of T-DNA complex, VirB4 | no data | 36 | 11 |
| | Bacteroides thetaiotaomicron VPI-5482 | BT4770 | | conserved protein found in conjugate transposon (NCBI ptt file) | sig. | 21 | 50 |
| | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_20990 | | conjugal transfer protein TraG | insig. | 22 | 53 |
| | Bacteroides stercoris CC31F | HMPREF1181_RS11500 | | TraG family conjugative transposon ATPase | insig. | 22 | 45 |
| | Bacteroides stercoris CC31F | HMPREF1181_RS09710 | | TraG family conjugative transposon ATPase | cofit | 24 | 33 |
| | Dechlorosoma suillum PS | Dsui_2577 | | type IV secretory pathway, VirB4 component | cofit | 25 | 15 |
| | Pedobacter sp. GW460-11-11-14-LB5 | CA265_RS20715 | | conjugal transfer protein TraG | insig. | 21 | 51 |
| | Sphingobium sp. HT1-2 | GFF4310 | | IncF plasmid conjugative transfer pilus assembly protein TraC | insig. | 23 | 33 |
| | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_3182 | | Bacteroides conjugation system ATPase, TraG family | insig. | 34 | 10 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF3144 | | IncF plasmid conjugative transfer pilus assembly protein TraC | insig. | 23 | 34 |