| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Caulobacter crescentus NA1000 | CCNA_01577 | | 2-keto-4-pentenoate hydratase family protein | sig. | 100 | 100 |
| o | Caulobacter crescentus NA1000 Δfur | CCNA_01577 | | 2-keto-4-pentenoate hydratase family protein | cofit | 100 | 100 |
| o | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS07645 | | fumarylacetoacetate hydrolase family protein | insig. | 52 | 99 |
| o | Lysobacter sp. OAE881 | ABIE51_RS02855 | | 2-keto-4-pentenoate hydratase | insig. | 51 | 94 |
| o | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.200.1 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | sig. | 47 | 97 |
| o | Sphingobium sp. HT1-2 | GFF1734 | | hypothetical protein | insig. | 44 | 93 |
| o | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2976 | | 2-keto-4-pentenoate hydratase | insig. | 43 | 90 |
| o | Azospirillum sp. SherDot2 | MPMX19_06121 | | hypothetical protein | insig. | 43 | 89 |
| | Azospirillum sp. SherDot2 | MPMX19_02815 | | 2-keto-4-pentenoate hydratase | insig. | 27 | 89 |
| o | Xanthobacter sp. DMC5 | GFF4544 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 33 | 84 |
| o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF2112 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 28 | 91 |
| o | Azospirillum brasilense Sp245 | AZOBR_RS15705 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 91 |
| | Herbaspirillum seropedicae SmR1 | HSERO_RS13395 | | hydratase | insig. | 25 | 76 |
| o | Burkholderia phytofirmans PsJN | BPHYT_RS28745 | | 2-keto-4-pentenoate hydratase | insig. | 26 | 90 |
| | Azospirillum sp. SherDot2 | MPMX19_05874 | | 2-keto-4-pentenoate hydratase | insig. | 27 | 83 |
| o | Pseudomonas segetis P6 | MPMX49_00433 | | 2-keto-4-pentenoate hydratase | insig. | 27 | 85 |
| o | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_864 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 24 | 88 |
| | Rhizobium sp. OAE497 | ABIE40_RS22530 | | fumarylacetoacetate hydrolase family protein | insig. | 27 | 75 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS10275 | | 2-oxopent-4-enoate hydratase | insig. | 43 | 29 |
| o | Cupriavidus basilensis FW507-4G11 | RR42_RS27885 | | 2-keto-4-pentenoate hydratase | insig. | 25 | 92 |
| o | Paraburkholderia graminis OAS925 | ABIE53_002319 | | 2-keto-4-pentenoate hydratase | insig. | 25 | 88 |
| o | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_05231 | | 2-keto-4-pentenoate hydratase | sig. | 24 | 88 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS34355 | | hydratase | insig. | 29 | 94 |
| o | Escherichia coli ECRC62 | BNILDI_23095 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 26 | 91 |
| o | Escherichia coli ECOR27 | NOLOHH_01685 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 26 | 91 |
| o | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_03321 | | 2-keto-4-pentenoate hydratase | insig. | 26 | 91 |
| o | Klebsiella pneumoniae MKP103 | KDGMDA_24060 | | 2-keto-4-pentenoate hydratase | insig. | 26 | 91 |
| o | Escherichia coli BL21 | ECD_00304 | | 2-keto-4-pentenoate hydratase | insig. | 26 | 91 |
| o | Escherichia coli ECOR38 | HEPCGN_07395 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 26 | 91 |
| o | Escherichia coli ECRC100 | OKFHMN_08770 | mhpD | 2-keto-4-pentenoate hydratase | cofit | 26 | 91 |
| o | Escherichia coli ECRC101 | MCAODC_28000 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 26 | 91 |
| o | Escherichia coli ECRC102 | NIAGMN_06825 | mhpD | 2-keto-4-pentenoate hydratase | cofit | 26 | 91 |
| o | Escherichia coli ECRC98 | JDDGAC_12425 | mhpD | 2-keto-4-pentenoate hydratase | insig. | 26 | 91 |
| o | Escherichia coli ECRC99 | KEDOAH_19200 | mhpD | 2-keto-4-pentenoate hydratase | no data | 26 | 91 |
| | Pseudomonas sp. BP01 | JOY50_RS14050 | | fumarylacetoacetate hydrolase family protein | no data | 31 | 35 |
| o | Pseudomonas fluorescens FW300-N2C3 | AO356_27440 | | 2-keto-4-pentenoate hydratase | insig. | 23 | 88 |
| o | Escherichia coli BW25113 | b0350 | mhpD | 2-keto-4-pentenoate hydratase (VIMSS) | insig. | 26 | 91 |
| o | Castellaniella sp019104865 MT123 | ABCV34_RS09290 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 25 | 91 |
| o | Variovorax sp. OAS795 | ABID97_RS27940 | | 2-keto-4-pentenoate hydratase | no data | 27 | 87 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_01663 | | 2-keto-4-pentenoate hydratase | no data | 28 | 85 |
| | Rhizobium sp. OAE497 | ABIE40_RS24835 | | fumarylacetoacetate hydrolase family protein | insig. | 27 | 55 |
| o | Klebsiella michiganensis M5al | BWI76_RS16910 | | 2-keto-4-pentenoate hydratase | strong | 25 | 81 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS34430 | | 2-keto-4-pentenoate hydratase | sig. | 23 | 86 |
| | Xanthobacter sp. DMC5 | GFF2802 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 40 | 26 |
| | Agrobacterium fabrum C58 | Atu4592 | | hypothetical protein | sig. | 27 | 91 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_833 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | insig. | 26 | 95 |
| | Rhizobium sp. OAE497 | ABIE40_RS20915 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 38 | 25 |
| | Variovorax sp. OAS795 | ABID97_RS15830 | | fumarylacetoacetate hydrolase family protein | insig. | 25 | 93 |
| | Shewanella sp. ANA-3 | Shewana3_3602 | | hydratase/decarboxylase family protein (RefSeq) | insig. | 21 | 97 |
| | Pseudomonas sp. BP01 | JOY50_RS02805 | | 2-keto-4-pentenoate hydratase | insig. | 23 | 89 |
| | Rhodopseudomonas palustris CGA009 | TX73_019460 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 34 | 31 |
| | Shewanella oneidensis MR-1 | SO4042 | | hydratase/decarboxylase family protein (NCBI ptt file) | insig. | 21 | 97 |
| | Pseudomonas putida KT2440 | PP_2652 | | putative Hydratase/decarboxylase | insig. | 24 | 87 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS07370 | | hydratase | insig. | 24 | 86 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS17015 | | 2-keto-4-pentenoate hydratase | insig. | 22 | 84 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_03564 | | 2-keto-4-pentenoate hydratase | insig. | 21 | 87 |
| | Azospirillum sp. SherDot2 | MPMX19_06796 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 29 | 57 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS04100 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 26 | 83 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_2504 | | 4-oxalocrotonate decarboxylase (EC 4.1.1.77) | sig. | 24 | 33 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS11195 | | hydratase | insig. | 24 | 88 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1824 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 29 | 57 |
| | Alteromonas macleodii MIT1002 | MIT1002_02100 | | 2-hydroxyhexa-2,4-dienoate hydratase | insig. | 28 | 49 |
| | Alteromonas macleodii MIT1002 | MIT1002_02097 | | 2-hydroxypent-2,4-dienoate hydratase | insig. | 34 | 29 |
| | Sphingobium sp. HT1-2 | GFF555 | | 2-keto-4-pentenoate hydratase (EC 4.2.1.80) | cofit | 27 | 86 |
| | Paraburkholderia graminis OAS925 | ABIE53_004692 | | 2-keto-4-pentenoate hydratase | insig. | 23 | 54 |
| | Escherichia coli BL21 | ECD_04226 | | 2-oxo-hept-3-ene-1,7-dioate hydratase | insig. | 27 | 95 |
| | Escherichia coli ECOR27 | NOLOHH_03735 | hpaH | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 27 | 95 |
| | Escherichia coli ECRC62 | BNILDI_07865 | hpaH | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | insig. | 27 | 95 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_03714 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | insig. | 27 | 95 |
| | Acidovorax sp. GW101-3H11 | Ac3H11_1483 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) | insig. | 29 | 57 |
| | Klebsiella pneumoniae MKP103 | KDGMDA_12100 | | 2-oxo-hepta-3-ene-1 7-dioic acid hydratase | insig. | 27 | 94 |
| | Ralstonia solanacearum GMI1000 | RS_RS21400 | | 2-keto-4-pentenoate hydratase | insig. | 28 | 83 |
| | Enterobacter sp. TBS_079 | MPMX20_00638 | | 2-oxo-hept-4-ene-1,7-dioate hydratase | sig. | 26 | 94 |
| | Klebsiella michiganensis M5al | BWI76_RS03850 | | 2-oxo-hepta-3-ene-1,7-dioic acid hydratase | sig. | 27 | 94 |