| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Alteromonas macleodii MIT1002 | MIT1002_03393 | | Type I restriction enzyme EcoKI specificity protein | insig. | 100 | 100 |
| o | Klebsiella michiganensis M5al | BWI76_RS03370 | | type I restriction-modification system subunit S | cofit | 37 | 99 |
| o | Bacteroides stercoris CC31F | HMPREF1181_RS13630 | | restriction endonuclease subunit S | insig. | 30 | 89 |
| o | Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 | GFF1000 | | Type I restriction-modification system, specificity subunit S (EC 3.1.21.3) | insig. | 32 | 88 |
| o | Shewanella oneidensis MR-1 | SO4264 | hsdS-2 | type I restriction-modification system, S subunit (NCBI ptt file) | insig. | 32 | 84 |
| o | Pseudomonas sp. RS175 | PFR28_03898 | | hypothetical protein | insig. | 30 | 84 |
| | Escherichia coli BW25113 | b4348 | hsdS | specificity determinant for hsdM and hsdR (NCBI) | sig. | 31 | 76 |
| o | Pantoea sp. MT58 | IAI47_16545 | | restriction endonuclease subunit S | cofit | 31 | 99 |
| o | Pseudomonas fluorescens FW300-N2E3 | AO353_11440 | | hypothetical protein | insig. | 29 | 97 |
| | Shewanella sp. ANA-3 | Shewana3_2960 | | restriction modification system DNA specificity subunit (RefSeq) | cofit | 27 | 84 |
| | Rhodanobacter sp. FW510-T8 | OKGIIK_14000 | | restriction endonuclease subunit S | insig. | 23 | 81 |
| o | Dickeya dianthicola ME23 | DZA65_RS04765 | | restriction endonuclease subunit S | insig. | 26 | 81 |
| | Shewanella sp. ANA-3 | Shewana3_3793 | | restriction modification system DNA specificity subunit (RefSeq) | insig. | 25 | 72 |
| | Paraburkholderia graminis OAS925 | ABIE53_000532 | | type I restriction enzyme S subunit | insig. | 35 | 38 |
| | Bacteroides thetaiotaomicron VPI-5482 | BT4520 | | Type I restriction enzyme EcoAI specificity protein (NCBI ptt file) | insig. | 27 | 44 |
| | Erwinia tracheiphila HP pepo 2.2 | IJEDHG_14265 | | Restriction endonuclease subunit S | no data | 25 | 80 |
| | Bacteroides ovatus ATCC 8483 | BACOVA_04170 | | hypothetical protein | insig. | 25 | 78 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS08650 | | restriction endonuclease subunit S | insig. | 25 | 86 |
| | Shewanella oneidensis MR-1 | SO0382 | hsdS-1 | type I restriction-modification system, S subunit (NCBI ptt file) | insig. | 19 | 78 |
| | Synechocystis sp000284455 PCC 6803 | SGL_RS01155 | | restriction endonuclease subunit S | sig. | 21 | 67 |
| | Escherichia coli ECRC100 | OKFHMN_11420 | hsdS | restriction endonuclease subunit S | cofit | 24 | 47 |
| | Escherichia coli ECRC101 | MCAODC_01935 | hsdS | restriction endonuclease subunit S | insig. | 24 | 47 |
| | Escherichia coli ECRC102 | NIAGMN_09270 | hsdS | restriction endonuclease subunit S | insig. | 24 | 47 |
| | Escherichia coli ECRC98 | JDDGAC_15015 | hsdS | restriction endonuclease subunit S | insig. | 24 | 47 |
| | Escherichia coli ECRC99 | KEDOAH_16725 | hsdS | restriction endonuclease subunit S | no data | 24 | 47 |
| | Herbaspirillum seropedicae SmR1 | HSERO_RS22785 | | Type I site-specific deoxyribonuclease specificity subunit | insig. | 21 | 79 |
| | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_0423 | | Restriction endonuclease S subunits | insig. | 24 | 80 |
| | Caulobacter crescentus NA1000 | CCNA_00657 | | type I restriction-modification system specificity subunit | insig. | 21 | 72 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_00657 | | type I restriction-modification system specificity subunit | cofit | 21 | 72 |
| | Cellulophaga baltica 18 | M666_RS02035 | | restriction endonuclease subunit S | cofit | 21 | 69 |
| | Sinorhizobium meliloti 1021 | SMc02295 | | specificity protein S | insig. | 26 | 32 |
| o | Shewanella oneidensis MR-1 | SO4264 | hsdS-2 | type I restriction-modification system, S subunit (NCBI ptt file) | insig. | 25 | 47 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.3938.1 | | Type I restriction-modification system, specificity subunit S (EC 3.1.21.3) | insig. | 27 | 50 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS02840 | | restriction endonuclease subunit S | sig. | 21 | 85 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_3107 | | Type I restriction-modification system, specificity subunit S (EC 3.1.21.3) | strong | 29 | 25 |
| | Phocaeicola dorei CL03T12C01 | ABI39_RS21405 | | restriction endonuclease subunit S | insig. | 22 | 55 |
| | Acinetobacter radioresistens SK82 | MPMX26_02869 | | hypothetical protein | strong | 24 | 28 |
| | Paraburkholderia graminis OAS925 | ABIE53_000532 | | type I restriction enzyme S subunit | insig. | 21 | 94 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS11945 | | restriction endonuclease subunit S | insig. | 23 | 81 |
| | Pontibacter actiniarum KMM 6156, DSM 19842 | CA264_06655 | | hypothetical protein | insig. | 23 | 68 |
| | Shewanella sp. ANA-3 | Shewana3_2960 | | restriction modification system DNA specificity subunit (RefSeq) | cofit | 26 | 47 |
| | Bacteroides thetaiotaomicron VPI-5482 | BT4543 | | putative type I restriction enzyme specificity protein (NCBI ptt file) | sig. | 23 | 44 |
| | Paraburkholderia graminis OAS925 | ABIE53_005411 | | type I restriction enzyme S subunit | strong | 21 | 73 |
| | Echinicola vietnamensis KMM 6221, DSM 17526 | Echvi_0381 | | Restriction endonuclease S subunits | insig. | 27 | 27 |
| | Rhodanobacter sp. FW510-T8 | OKGIIK_13430 | | Methylase-S domain-containing protein | insig. | 24 | 37 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c30700 | | putative type I restriction-modification system, S subunit | insig. | 21 | 62 |
| | Pedobacter sp. GW460-11-11-14-LB5 | CA265_RS20485 | | hypothetical protein | cofit | 25 | 34 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_02136 | | hypothetical protein | insig. | 24 | 36 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS11945 | | restriction endonuclease subunit S | insig. | 23 | 27 |
| | Acinetobacter radioresistens SK82 | MPMX26_02869 | | hypothetical protein | strong | 24 | 39 |
| | Alteromonas macleodii MIT1002 | MIT1002_04091 | | EcoKI restriction-modification system protein HsdS | insig. | 25 | 33 |