| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU2286 | | hydrogenase, CooM subunit, putative (TIGR) | strong | 100 | 100 |
| o | Desulfovibrio vulgaris Miyazaki F | DvMF_3194 | | NADH dehydrogenase (quinone) (RefSeq) | strong | 62 | 100 |
| o | Rhodospirillum rubrum S1H | Rru_A1420 | | NADH dehydrogenase (quinone) (NCBI) | insig. | 43 | 99 |
| | Rhodospirillum rubrum S1H | Rru_A0315 | | NADH dehydrogenase (quinone) (NCBI) | insig. | 36 | 34 |
| | Escherichia coli BW25113 | b2482 | hyfB | NADH dehydrogenase subunit N (NCBI) | insig. | 32 | 34 |
| | Escherichia coli ECRC100 | OKFHMN_21895 | hyfB | hydrogenase 4 subunit B | cofit | 32 | 34 |
| | Escherichia coli ECRC101 | MCAODC_12425 | hyfB | hydrogenase 4 subunit B | insig. | 32 | 34 |
| | Escherichia coli ECRC102 | NIAGMN_19665 | hyfB | hydrogenase 4 subunit B | insig. | 32 | 34 |
| | Escherichia coli ECRC98 | JDDGAC_25840 | hyfB | hydrogenase 4 subunit B | insig. | 32 | 34 |
| | Escherichia coli ECRC99 | KEDOAH_06325 | hyfB | hydrogenase 4 subunit B | no data | 32 | 34 |
| | Escherichia coli BL21 | ECD_02374 | | hydrogenase 4, membrane subunit | insig. | 33 | 32 |
| | Escherichia coli ECRC62 | BNILDI_12365 | hyfB | hydrogenase 4 subunit B | insig. | 32 | 34 |
| | Escherichia coli ECOR27 | NOLOHH_13555 | hyfB | hydrogenase 4 subunit B | insig. | 32 | 34 |
| | Escherichia coli ECOR38 | HEPCGN_21065 | hyfB | hydrogenase 4 subunit B | insig. | 33 | 32 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1601 | | putative monovalent cation/H+ antiporter subunit D (RefSeq) | strong | 34 | 31 |
| | Rhodospirillum rubrum S1H | Rru_A1634 | | NADH dehydrogenase (quinone) (NCBI) | cofit | 35 | 31 |
| | Rhodospirillum rubrum S1H | Rru_A0315 | | NADH dehydrogenase (quinone) (NCBI) | insig. | 30 | 39 |
| | Pseudomonas stutzeri RCH2 | Psest_3887 | | Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit | insig. | 32 | 31 |
| | Rhodospirillum rubrum S1H | Rru_A0314 | | NADH dehydrogenase (quinone) (NCBI) | cofit | 33 | 33 |
| | Azospirillum sp. SherDot2 | MPMX19_03515 | | Hydrogenase-4 component B | insig. | 35 | 37 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.589.1 | | hypothetical protein | insig. | 33 | 35 |
| | Pseudomonas stutzeri RCH2 | Psest_3887 | | Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit | insig. | 33 | 31 |
| | Rhodospirillum rubrum S1H | Rru_A0320 | | NADH dehydrogenase (quinone) (NCBI) | insig. | 34 | 27 |
| | Sinorhizobium meliloti 1021 | SMa1541 | | monovalent cation/H+ antiporter subunit D | insig. | 32 | 32 |
| | Dickeya dianthicola ME23 | DZA65_RS07855 | | hydrogenase 4 subunit B | insig. | 33 | 33 |
| | Dickeya dadantii 3937 | DDA3937_RS07470 | | hydrogenase 4 subunit B | insig. | 33 | 34 |
| | Dickeya dianthicola 67-19 | HGI48_RS07345 | | hydrogenase 4 subunit B | insig. | 33 | 33 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS01070 | | hydrogenase 4 subunit B | cofit | 34 | 32 |
| | Sinorhizobium meliloti 1021 | SMa1541 | | monovalent cation/H+ antiporter subunit D | insig. | 34 | 31 |
| | Rhodopseudomonas palustris CGA009 | TX73_014445 | | monovalent cation/H+ antiporter subunit A | insig. | 33 | 33 |
| | Azospirillum sp. SherDot2 | MPMX19_03511 | | NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic | insig. | 31 | 35 |
| | Lysobacter sp. OAE881 | ABIE51_RS09895 | | monovalent cation/H+ antiporter subunit A | insig. | 32 | 32 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS07595 | | monovalent cation/H+ antiporter subunit A | strong | 31 | 33 |
| | Pseudomonas orientalis W4I3 | QF045_RS18565 | | monovalent cation/H+ antiporter subunit A | insig. | 31 | 35 |
| | Xanthobacter sp. DMC5 | GFF2057 | | Hydrogenase-4 component B | insig. | 30 | 40 |
| | Rhodanobacter sp. FW510-T8 | OKGIIK_14045 | | Cation:proton antiporter | sig. | 31 | 35 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_2201 | | multisubunit sodium/proton antiporter, MrpD subunit (TC 2.A.63.1) | strong | 28 | 31 |
| | Escherichia coli ECRC100 | OKFHMN_21875 | hyfF | hydrogenase 4 subunit F | cofit | 30 | 35 |
| | Escherichia coli ECRC101 | MCAODC_12405 | hyfF | hydrogenase 4 subunit F | insig. | 30 | 35 |
| | Escherichia coli ECRC102 | NIAGMN_19645 | hyfF | hydrogenase 4 subunit F | insig. | 30 | 35 |
| | Escherichia coli ECRC98 | JDDGAC_25820 | hyfF | hydrogenase 4 subunit F | insig. | 30 | 35 |
| | Azospirillum sp. SherDot2 | MPMX19_04697 | | NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic | cofit | 32 | 35 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1601 | | putative monovalent cation/H+ antiporter subunit D (RefSeq) | strong | 31 | 32 |
| | Escherichia coli HS(pFamp)R (ATCC 700891) | OHPLBJKB_01240 | | NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic | insig. | 29 | 35 |
| | Escherichia coli BL21 | ECD_02378 | | hydrogenase 4, membrane subunit | insig. | 29 | 35 |
| | Escherichia coli BW25113 | b2486 | hyfF | NADH dehydrogenase subunit N (NCBI) | insig. | 29 | 35 |
| | Escherichia coli ECOR27 | NOLOHH_13530 | hyfF | hydrogenase 4 subunit F | insig. | 29 | 35 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS00910 | | monovalent cation/H+ antiporter subunit A | cofit | 31 | 36 |
| | Rhodospirillum rubrum S1H | Rru_A0314 | | NADH dehydrogenase (quinone) (NCBI) | cofit | 31 | 32 |
| | Escherichia coli ECRC99 | KEDOAH_06345 | hyfF | hydrogenase 4 subunit F | no data | 29 | 35 |
| | Xanthobacter sp. DMC5 | GFF2057 | | Hydrogenase-4 component B | insig. | 33 | 34 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_2201 | | multisubunit sodium/proton antiporter, MrpD subunit (TC 2.A.63.1) | strong | 27 | 32 |
| | Escherichia coli ECOR38 | HEPCGN_21045 | hyfF | hydrogenase 4 subunit F | insig. | 30 | 35 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_03684 | | NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic | insig. | 29 | 37 |
| | Mycobacterium tuberculosis H37Rv | Rv0083 | | Probable oxidoreductase | insig. | 33 | 32 |
| | Rhodospirillum rubrum S1H | Rru_A1634 | | NADH dehydrogenase (quinone) (NCBI) | cofit | 32 | 30 |
| | Bacteroides stercoris CC31F | HMPREF1181_RS09035 | | NADH-quinone oxidoreductase subunit L | sig. | 29 | 32 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS07335 | | monovalent cation/H+ antiporter subunit A | cofit | 30 | 36 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS17195 | | proton-conducting transporter membrane subunit | insig. | 34 | 26 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS17720 | | NADH dehydrogenase | insig. | 34 | 26 |
| | Rhodanobacter sp. FW510-T8 | OKGIIK_07585 | hyfB | hydrogenase 4 subunit B | insig. | 37 | 28 |
| | Kangiella aquimarina DSM 16071 | B158DRAFT_2202 | | multisubunit sodium/proton antiporter, MrpD subunit (TC 2.A.63.1) | strong | 30 | 31 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_21470 | | cation:proton antiporter | strong | 30 | 36 |
| | Rhodanobacter denitrificans FW104-10B01 | LRK54_RS17305 | | hydrogenase 4 subunit B | strong | 37 | 28 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_5822 | | Na(+) H(+) antiporter subunit A / Na(+) H(+) antiporter subunit B | strong | 30 | 37 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c12270 | | monovalent cation/proton antiporter subunit A/B | no data | 32 | 32 |
| | Pseudomonas simiae WCS417 | PS417_16830 | | monovalent cation/H+ antiporter subunit A | strong | 29 | 36 |
| | Rhodanobacter denitrificans MT42 | LRK55_RS17530 | | hydrogenase 4 subunit B | cofit | 37 | 28 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_538 | | Na(+) H(+) antiporter subunit A; Na(+) H(+) antiporter subunit B | insig. | 31 | 37 |
| | Acinetobacter baumannii LAC-4 | RR41_RS17265 | | monovalent cation/H+ antiporter subunit A | no data | 29 | 33 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS01070 | | hydrogenase 4 subunit B | cofit | 31 | 33 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF2658 | | Na(+) H(+) antiporter subunit A; Na(+) H(+) antiporter subunit B | cofit | 30 | 32 |
| | Sinorhizobium meliloti 1021 | SMc03179 | | monovalent cation/H+ antiporter subunit A | strong | 29 | 33 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_3252 | | multisubunit potassium/proton antiporter, PhaA subunit / multisubunit potassium/proton antiporter, PhaB subunit | insig. | 29 | 32 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_3252 | | multisubunit potassium/proton antiporter, PhaA subunit / multisubunit potassium/proton antiporter, PhaB subunit | insig. | 29 | 32 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_23280 | | cation:proton antiporter | insig. | 30 | 37 |
| | Escherichia coli BW25113 | b2482 | hyfB | NADH dehydrogenase subunit N (NCBI) | insig. | 29 | 31 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS08480 | | monovalent cation/H+ antiporter subunit A | insig. | 32 | 34 |
| | Dickeya dianthicola 67-19 | HGI48_RS07325 | | hydrogenase 4 subunit F | insig. | 32 | 27 |
| | Escherichia coli ECRC62 | BNILDI_12365 | hyfB | hydrogenase 4 subunit B | insig. | 29 | 31 |
| | Pseudomonas sp. DMC3 | GFF1738 | | NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic | cofit | 27 | 40 |
| | Dickeya dadantii 3937 | DDA3937_RS07450 | | hydrogenase 4 subunit F | insig. | 32 | 27 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3805 | | NADH dehydrogenase (quinone) (RefSeq) | no data | 31 | 30 |
| | Escherichia coli ECRC62 | BNILDI_12335 | hyfF | hydrogenase 4 subunit F | insig. | 30 | 35 |
| | Escherichia coli ECRC100 | OKFHMN_21895 | hyfB | hydrogenase 4 subunit B | cofit | 29 | 31 |
| | Escherichia coli ECRC101 | MCAODC_12425 | hyfB | hydrogenase 4 subunit B | insig. | 29 | 31 |
| | Escherichia coli ECRC102 | NIAGMN_19665 | hyfB | hydrogenase 4 subunit B | insig. | 29 | 31 |
| | Escherichia coli ECRC98 | JDDGAC_25840 | hyfB | hydrogenase 4 subunit B | insig. | 29 | 31 |
| | Escherichia coli ECRC99 | KEDOAH_06325 | hyfB | hydrogenase 4 subunit B | no data | 29 | 31 |
| | Escherichia coli ECOR27 | NOLOHH_13555 | hyfB | hydrogenase 4 subunit B | insig. | 29 | 31 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c12270 | | monovalent cation/proton antiporter subunit A/B | no data | 30 | 30 |
| | Sinorhizobium meliloti 1021 | SMa1538 | | monovalent cation/H+ antiporter subunit D | insig. | 30 | 32 |
| | Escherichia coli BL21 | ECD_02374 | | hydrogenase 4, membrane subunit | insig. | 29 | 31 |
| | Dickeya dianthicola ME23 | DZA65_RS07835 | | hydrogenase 4 subunit F | insig. | 32 | 27 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.589.1 | | hypothetical protein | insig. | 30 | 32 |
| | Escherichia coli ECOR38 | HEPCGN_21065 | hyfB | hydrogenase 4 subunit B | insig. | 30 | 30 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS11710 | | hydrogenase 4 subunit B | insig. | 37 | 28 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF2658 | | Na(+) H(+) antiporter subunit A; Na(+) H(+) antiporter subunit B | cofit | 30 | 33 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_1603 | | putative monovalent cation/H+ antiporter subunit D (RefSeq) | strong | 34 | 28 |
| | Pseudomonas putida KT2440 | PP_2230 | | putative K(+)/H(+) antiporter subunit A/B | insig. | 30 | 37 |