Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| Dickeya dianthicola 67-19 | HGI48_RS03935 | | conjugal transfer protein TraG | insig. | 100 | 100 |
o | Ralstonia solanacearum GMI1000 | RS_RS12965 | | conjugal transfer protein TraG | insig. | 89 | 100 |
o | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4174 | | Type IV secretion system protein VirD4 | insig. | 90 | 100 |
o | Variovorax sp. SCN45 | GFF4851 | | Coupling protein VirD4, ATPase required for T-DNA transfer | cofit | 88 | 100 |
| Variovorax sp. SCN45 | GFF4412 | | Coupling protein VirD4, ATPase required for T-DNA transfer | cofit | 88 | 100 |
o | Shewanella sp. ANA-3 | Shewana3_1280 | | conjugal transfer coupling protein TraG (RefSeq) | insig. | 89 | 100 |
| Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4433 | | Type IV secretion system protein VirD4 | insig. | 87 | 100 |
o | Castellaniella sp019104865 MT123 | ABCV34_RS00145 | | conjugal transfer protein TraG | insig. | 87 | 100 |
| Variovorax sp. SCN45 | GFF6581 | | Coupling protein VirD4, ATPase required for T-DNA transfer | insig. | 88 | 100 |
| Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF3455 | | Type IV secretion system protein VirD4 | insig. | 82 | 100 |
| Dickeya dadantii 3937 | DDA3937_RS03985 | | conjugal transfer protein TraG | insig. | 78 | 96 |
| Dickeya dianthicola 67-19 | HGI48_RS08410 | | conjugal transfer protein TraG | insig. | 78 | 95 |
o | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2910 | | Type IV secretory pathway, VirD4 components | insig. | 71 | 92 |
| Sphingomonas koreensis DSMZ 15582 | Ga0059261_0391 | | Type IV secretory pathway, VirD4 components | insig. | 73 | 87 |
| Sphingomonas koreensis DSMZ 15582 | Ga0059261_3070 | | Type IV secretory pathway, VirD4 components | insig. | 71 | 92 |
| Sphingomonas koreensis DSMZ 15582 | Ga0059261_2574 | | Type IV secretory pathway, VirD4 components | insig. | 66 | 100 |
o | Rhodopseudomonas palustris CGA009 | TX73_011535 | | conjugal transfer protein TraG | cofit | 65 | 100 |
| Rhodopseudomonas palustris CGA009 | TX73_021410 | | conjugal transfer protein TraG | insig. | 65 | 100 |
o | Marinobacter adhaerens HP15 | HP15_p42g23 | | TRAG family protein | insig. | 40 | 81 |
o | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS03185 | | type IV secretory system conjugative DNA transfer family protein | insig. | 32 | 81 |
| Agrobacterium fabrum C58 | Atu4858 | | virulence virD4-like protein | cofit | 35 | 67 |
| Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.2984.1 | | Type IV secretion system protein VirD4 | insig. | 30 | 74 |
| Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.2039.1 | | hypothetical protein | insig. | 30 | 75 |
| Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11495 | | type IV secretory system conjugative DNA transfer family protein | insig. | 30 | 75 |
| Agrobacterium fabrum C58 | Atu6184 | | virA/G regulated protein | insig. | 29 | 79 |
| Burkholderia phytofirmans PsJN | BPHYT_RS32145 | | type IV secretory pathway protein VirD4 | cofit | 31 | 67 |
| Paraburkholderia sabiae LMG 24235 | QEN71_RS06750 | | type IV secretory system conjugative DNA transfer family protein | cofit | 30 | 66 |
| Burkholderia phytofirmans PsJN | BPHYT_RS00820 | | type IV secretory pathway protein VirD4 | insig. | 30 | 65 |
| Escherichia coli ECOR27 | NOLOHH_00290 | | conjugal transfer protein TraG | cofit | 27 | 72 |
| Paraburkholderia sabiae LMG 24235 | QEN71_RS40890 | | type IV secretory system conjugative DNA transfer family protein | insig. | 29 | 64 |
| Phaeobacter inhibens DSM 17395 | PGA1_c22970 | | putative protein VirD4 | insig. | 27 | 76 |
| Dinoroseobacter shibae DFL-12 | Dshi_3655 | | TRAG family protein (RefSeq) | insig. | 26 | 68 |
| Dinoroseobacter shibae DFL-12 | Dshi_3988 | | TRAG family protein (RefSeq) | insig. | 26 | 68 |
| Ralstonia solanacearum UW163 | UW163_RS23885 | | hypothetical protein | insig. | 26 | 66 |
| Escherichia coli ECOR38 | HEPCGN_00335 | | conjugal transfer protein TraG | cofit | 22 | 83 |
| Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.892.1 | | Coupling protein VirD4, ATPase required for T-DNA transfer | insig. | 26 | 38 |
| Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.889.1 | | Type IV secretion system protein VirD4 | insig. | 40 | 26 |
| Sinorhizobium meliloti 1021 | SMa0929 | | conjugal transfer protein TraG | insig. | 26 | 61 |
| Agrobacterium fabrum C58 | Atu6124 | | conjugative transfer protein, Coupling protein | insig. | 26 | 62 |
| Agrobacterium fabrum C58 | Atu5108 | | conjugal transfer coupling protein TraG | sig. | 23 | 60 |
| Bifidobacterium breve UCC2003 | BBR_RS18385 | | conjugal transfer protein TraG | insig. | 22 | 69 |
| Azospirillum brasilense Sp245 | AZOBR_RS33175 | | exported protein of unknown function | insig. | 23 | 49 |
| Rhizobium sp. OAE497 | ABIE40_RS12455 | | type IV secretory system conjugative DNA transfer family protein | insig. | 27 | 27 |
| Caulobacter crescentus NA1000 | CCNA_00475 | | bacterial conjugation protein, ATP binding domain trwB | cofit | 24 | 56 |
| Caulobacter crescentus NA1000 Δfur | CCNA_00475 | | bacterial conjugation protein, ATP binding domain trwB | cofit | 24 | 56 |
| Azospirillum brasilense Sp245 | AZOBR_RS33175 | | exported protein of unknown function | insig. | 31 | 14 |