| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Sinorhizobium meliloti 1021 | SMa0929 | | conjugal transfer protein TraG | insig. | 100 | 100 |
| o | Agrobacterium fabrum C58 | Atu5108 | | conjugal transfer coupling protein TraG | sig. | 84 | 100 |
| | Agrobacterium fabrum C58 | Atu6124 | | conjugative transfer protein, Coupling protein | insig. | 46 | 87 |
| | Escherichia coli ECOR27 | NOLOHH_00290 | | conjugal transfer protein TraG | insig. | 26 | 66 |
| | Xanthobacter sp. DMC5 | GFF2618 | | Conjugal transfer protein TraG | insig. | 28 | 79 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2574 | | Type IV secretory pathway, VirD4 components | insig. | 28 | 64 |
| | Rhodopseudomonas palustris CGA009 | TX73_021410 | | conjugal transfer protein TraG | insig. | 27 | 79 |
| | Xanthobacter sp. DMC5 | GFF3645 | | Conjugal transfer protein TraG | insig. | 27 | 79 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS00145 | | conjugal transfer protein TraG | insig. | 27 | 62 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_2910 | | Type IV secretory pathway, VirD4 components | insig. | 25 | 63 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_3070 | | Type IV secretory pathway, VirD4 components | insig. | 25 | 63 |
| | Dickeya dadantii 3937 | DDA3937_RS03985 | | conjugal transfer protein TraG | insig. | 26 | 64 |
| | Dickeya dianthicola 67-19 | HGI48_RS03935 | | conjugal transfer protein TraG | insig. | 26 | 64 |
| | Rhodopseudomonas palustris CGA009 | TX73_011535 | | conjugal transfer protein TraG | cofit | 25 | 79 |
| | Dickeya dianthicola 67-19 | HGI48_RS08410 | | conjugal transfer protein TraG | insig. | 26 | 62 |
| | Shewanella sp. ANA-3 | Shewana3_1280 | | conjugal transfer coupling protein TraG (RefSeq) | insig. | 25 | 79 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4174 | | Type IV secretion system protein VirD4 | insig. | 26 | 62 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_12815 | | conjugal transfer protein TraG | strong | 26 | 62 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0391 | | Type IV secretory pathway, VirD4 components | insig. | 25 | 63 |
| | Variovorax sp. SCN45 | GFF4412 | | Coupling protein VirD4, ATPase required for T-DNA transfer | cofit | 26 | 62 |
| | Ralstonia solanacearum GMI1000 | RS_RS12965 | | conjugal transfer protein TraG | insig. | 26 | 62 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF4433 | | Type IV secretion system protein VirD4 | insig. | 25 | 62 |
| | Variovorax sp. SCN45 | GFF6581 | | Coupling protein VirD4, ATPase required for T-DNA transfer | insig. | 26 | 62 |
| | Variovorax sp. SCN45 | GFF4851 | | Coupling protein VirD4, ATPase required for T-DNA transfer | cofit | 26 | 62 |
| | Agrobacterium fabrum C58 | Atu4858 | | virulence virD4-like protein | cofit | 24 | 62 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF3455 | | Type IV secretion system protein VirD4 | insig. | 26 | 62 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS06750 | | type IV secretory system conjugative DNA transfer family protein | cofit | 23 | 62 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS32145 | | type IV secretory pathway protein VirD4 | cofit | 23 | 62 |
| | Escherichia coli ECOR38 | HEPCGN_00335 | | conjugal transfer protein TraG | insig. | 23 | 62 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS03185 | | type IV secretory system conjugative DNA transfer family protein | insig. | 25 | 63 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.2984.1 | | Type IV secretion system protein VirD4 | insig. | 23 | 60 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS00820 | | type IV secretory pathway protein VirD4 | insig. | 22 | 60 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.2039.1 | | hypothetical protein | insig. | 21 | 60 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS40890 | | type IV secretory system conjugative DNA transfer family protein | insig. | 21 | 68 |
| | Marinobacter adhaerens HP15 | HP15_p42g23 | | TRAG family protein | insig. | 23 | 63 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.892.1 | | Coupling protein VirD4, ATPase required for T-DNA transfer | insig. | 24 | 39 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3988 | | TRAG family protein (RefSeq) | insig. | 23 | 62 |
| | Dinoroseobacter shibae DFL-12 | Dshi_3655 | | TRAG family protein (RefSeq) | insig. | 23 | 62 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11495 | | type IV secretory system conjugative DNA transfer family protein | insig. | 21 | 60 |
| | Phaeobacter inhibens DSM 17395 | PGA1_c22970 | | putative protein VirD4 | insig. | 22 | 65 |
| | Caulobacter crescentus NA1000 | CCNA_00475 | | bacterial conjugation protein, ATP binding domain trwB | cofit | 29 | 19 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_00475 | | bacterial conjugation protein, ATP binding domain trwB | cofit | 29 | 19 |
| | Agrobacterium fabrum C58 | Atu6184 | | virA/G regulated protein | insig. | 21 | 64 |
| | Rhizobium sp. OAE497 | ABIE40_RS12455 | | type IV secretory system conjugative DNA transfer family protein | insig. | 22 | 29 |