Pairwise Alignments
Query, 1096 a.a., histidine kinase from Pseudomonas fluorescens FW300-N2E3
Subject, 855 a.a., Putative diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (NCBI) from Rhodospirillum rubrum S1H
Score = 421 bits (1081), Expect = e-121
Identities = 256/703 (36%), Positives = 382/703 (54%), Gaps = 21/703 (2%)
Query: 400 DITDQVEREQRLITSEEKFASLFQVSPDPICVTRQDTGQFIEINPAFTQIFGWTT-TDVI 458
D+T QV E L +E K+ ++F+ + + I + D G ++++NPA +I+G+ + ++I
Sbjct: 148 DMTAQVAAENALRAAEGKYRAIFENAVEGIYQSTPD-GTYLDVNPALARIYGYASPAELI 206
Query: 459 DRSADEIGLWDASVKRQQRIEQVIREQA-LNNVAIIVHHKDGQPLTCVISSRQISVGNEP 517
D ++ ++++RE + + V DG + ++R + +
Sbjct: 207 AHFTDIASQLYVDGTKRGEFQRLMRETGEVRDFTAEVWRCDGHTIWITENARCVRGPDNE 266
Query: 518 CIVT--TLRDITQQQRSEAALKASEEKFAKAFHSSPDAITITERDSGRYLEVNDGFCRLT 575
+ T+ DIT Q++S+ ++ K F S + I + ERD VN F +T
Sbjct: 267 VLYYEGTVEDITAQRQSQETMRL----LGKVFTSIAEGIVLLERDL-TVRTVNPAFEAMT 321
Query: 576 GYSADEVVGKTVYQIGIWAEEKQRSTLLAALKAKGRVHHQEMLGRNKRGEILTVEVSIEP 635
G ++ G+ E +AA + + E+ G + G + E+++
Sbjct: 322 GLLRQDMEGRPARLFASGLHENDFLATVAAEVDRAGLWRGEIWGERREGPPYSGEMTVTA 381
Query: 636 ITLN--ETACLLMTARDVSLLKNAEAQIRHLAYHDPLTNLPNRALLMDRLSQQIALLKRH 693
+ ET + D++ K E IR A D LT+LPNR L+MDRL Q + +R
Sbjct: 382 VRTRAGETTHYVAAITDITKRKRDEEHIRFQANFDMLTHLPNRHLIMDRLEQAMHQAQRT 441
Query: 694 NLRGALLFLDLDHFKHINDSLGHPVGDTVLKIITARLEASVRMEDTVARLGGDEFVVLLS 753
+ ++FLDLD FK INDS GH GD VLK+ RL VR+ D+V RLGGDEF+V+LS
Sbjct: 442 GRQVCVMFLDLDRFKQINDSYGHSAGDEVLKLTARRLRNCVRISDSVGRLGGDEFIVILS 501
Query: 754 GLEGTRNEVSEQVRELADTLRELLSEPMFLDGQRLQVTPSIGVALIPDHGSTPTDLLKRA 813
+E +A+ + LSEP + G + PS+G+ PDHG T DLL+ A
Sbjct: 502 NVEDQHAGAY-----IAEKILYSLSEPFPIGGTEVFCIPSVGITYFPDHGETAPDLLRNA 556
Query: 814 DIALYRAKDSGRNATQMFHNTMQKAASERLRMETDLRLALSRGEFSVQYQPQVDALDGRI 873
D+A+Y AK G +F M + + L ME+DLR AL+R EF + +QP+V A D +
Sbjct: 557 DVAMYHAKQGGERRYAIFTPDMARRSLALLTMESDLRHALARDEFELHFQPKVRA-DLTL 615
Query: 874 IGAEALVRWRHPRLGEQSPSEFIKVLEDSGLILEVGAWILDETCQAFRQLIDRGLIDPLN 933
IGAEAL+RWRHP G +P +FI + E+SGLIL +G W L E C GL P
Sbjct: 616 IGAEALIRWRHPEKGLINPGDFIPLAEESGLILPIGRWTLREACDRVMSWRAEGLTIP-- 673
Query: 934 FSLCVNISPRQFRQNDFVERIEQTLSSHGLPYSLLKLEVTEGIVIQNLDDTISKMRRLKK 993
S+ VN+SPRQF+ VE + Q L GL L LE+TE ++ +++ + +R LK
Sbjct: 674 -SVSVNVSPRQFQDQTLVETVRQILVETGLEPEALDLEITETVMTGDVEHAVGTLRALKD 732
Query: 994 LGVSFAMDDFGTGYSSLTYLKRLPVDTLKIDQSFIRDATSDPNDAEIIRAIVAMARSLEL 1053
LGV+ ++DDFGTGYSSL YLK P+DTLKIDQ+F+RD DA I+ I+A+AR+L
Sbjct: 733 LGVTLSIDDFGTGYSSLNYLKTFPIDTLKIDQTFVRDVLHSGKDAAIVSTIIALARNLGF 792
Query: 1054 TVIAEGVETTEQLDFLQGLGCHLYQGYLHSQPLPLEGFLKLLK 1096
+V+AEGVE EQ +FL C +QG+L+S+PLP F L+
Sbjct: 793 SVVAEGVEEIEQAEFLGSRDCQNFQGFLYSRPLPPAEFTTFLR 835
Score = 46.6 bits (109), Expect = 8e-09
Identities = 40/163 (24%), Positives = 68/163 (41%), Gaps = 20/163 (12%)
Query: 494 VHHKDGQPLTCVISSRQISVGNEPCIVTTLRDITQQQRSEAALKASEEKFAKAFHSSPDA 553
+ H G + + G P +V RD+T Q +E AL+A+E K+ F ++ +
Sbjct: 117 IRHISGSERVYSLRFSPLDNGPHPLLVVITRDMTAQVAAENALRAAEGKYRAIFENAVEG 176
Query: 554 ITITERDSGRYLEVNDGFCRLTGYS--ADEVVGKTVYQIGIWAEEKQRSTLLAALKAKGR 611
I + D G YL+VN R+ GY+ A+ + T ++ + +R ++ G
Sbjct: 177 IYQSTPD-GTYLDVNPALARIYGYASPAELIAHFTDIASQLYVDGTKRGEFQRLMRETGE 235
Query: 612 VH-----------HQEMLGRNKR------GEILTVEVSIEPIT 637
V H + N R E+L E ++E IT
Sbjct: 236 VRDFTAEVWRCDGHTIWITENARCVRGPDNEVLYYEGTVEDIT 278
Score = 36.6 bits (83), Expect = 8e-06
Identities = 86/439 (19%), Positives = 162/439 (36%), Gaps = 47/439 (10%)
Query: 182 DGLISEANQYFESLFGWPVHSVIGRTTLELGLWVNPEQRAKLVKATKAK-----GELVSM 236
DG+I AN G + ++IG L ++ P + K + G V
Sbjct: 57 DGVIVHANATLAGQIGETIDTLIGSP---LTSYLTPADALAVQKVVEGAIALHGGVPVRR 113
Query: 237 EVQFRASNGQVHDGILSAQKVELEGQPYLLSTFLDTSERKLAEQALKDSQERLDLALDSA 296
R +G L ++ P L+ D + + AE AL+ ++ + ++A
Sbjct: 114 IAHIRHISGSERVYSLRFSPLDNGPHPLLVVITRDMTAQVAAENALRAAEGKYRAIFENA 173
Query: 297 QLGTWDWHIPSGMLYGSARAAQLHGL-DPIPFHEPFDAFFEGVPDDERDSMRDAYRSLRE 355
G + L + A+++G P F + D + R ++ L
Sbjct: 174 VEGIYQSTPDGTYLDVNPALARIYGYASPAELIAHFTDIASQLYVD--GTKRGEFQRLMR 231
Query: 356 GPAGNYQLTYRVQLPDGSSRYLESRARLYRDESGVPLRMAGTLLDITDQVEREQRLITSE 415
T V DG + ++ AR R L GT+ DIT Q + ++ +
Sbjct: 232 ETGEVRDFTAEVWRCDGHTIWITENARCVRGPDNEVLYYEGTVEDITAQRQSQETMRLLG 291
Query: 416 EKFASLFQVSPDPICVTRQDTGQFIEINPAFTQIFGWTTTDVIDRSADEIGLWDASVKRQ 475
+ F S+ + I + +D +NPAF + G D+ R A L+ + +
Sbjct: 292 KVFTSI----AEGIVLLERDL-TVRTVNPAFEAMTGLLRQDMEGRPA---RLFASGLHEN 343
Query: 476 QRIEQVIRE---QALNNVAIIVHHKDGQPLT--CVISSRQISVGNEPCIVTTLRDITQQQ 530
+ V E L I ++G P + +++ + G V + DIT+++
Sbjct: 344 DFLATVAAEVDRAGLWRGEIWGERREGPPYSGEMTVTAVRTRAGETTHYVAAITDITKRK 403
Query: 531 RSEAALKASE---------------EKFAKAFHSSPDA---ITITERDSGRYLEVNDGFC 572
R E ++ ++ +A H + + + D R+ ++ND +
Sbjct: 404 RDEEHIRFQANFDMLTHLPNRHLIMDRLEQAMHQAQRTGRQVCVMFLDLDRFKQINDSY- 462
Query: 573 RLTGYSA-DEVVGKTVYQI 590
G+SA DEV+ T ++
Sbjct: 463 ---GHSAGDEVLKLTARRL 478
Score = 28.9 bits (63), Expect = 0.002
Identities = 36/170 (21%), Positives = 66/170 (38%), Gaps = 10/170 (5%)
Query: 118 DGSLHWLEINGSLI--PDKQGRPRMIGVIREITHQRQREQALSSSEKRFATLFHLCPNMV 175
DG W+ N + PD + G + +IT QRQ ++ + K F + + +V
Sbjct: 247 DGHTIWITENARCVRGPDNEVL-YYEGTVEDITAQRQSQETMRLLGKVFTS---IAEGIV 302
Query: 176 LLTRQEDGLISEANQYFESLFGWPVHSVIGRTTLELGLWVNPEQRAKLVKATKAKGELVS 235
LL R D + N FE++ G + GR ++ V A + L
Sbjct: 303 LLER--DLTVRTVNPAFEAMTGLLRQDMEGRPARLFASGLHENDFLATVAAEVDRAGLWR 360
Query: 236 MEVQFRASNGQVHDGILSAQKVELEG--QPYLLSTFLDTSERKLAEQALK 283
E+ G + G ++ V + ++ D ++RK E+ ++
Sbjct: 361 GEIWGERREGPPYSGEMTVTAVRTRAGETTHYVAAITDITKRKRDEEHIR 410