Pairwise Alignments
Query, 1056 a.a., heavy metal efflux pump (cobalt-zinc-cadmium) from Pseudomonas stutzeri RCH2
Subject, 1066 a.a., CzcA family heavy metal efflux protein (RefSeq) from Shewanella amazonensis SB2B
Score = 726 bits (1874), Expect = 0.0
Identities = 424/1078 (39%), Positives = 638/1078 (59%), Gaps = 47/1078 (4%)
Query: 1 MFERIIRFSIEHRWLVMLAVLGMAALGAYSYQKLPIDAVPDITNVQVQINTAAPGYSPLE 60
M +++I ++ R +V++ ++G A A KL +DA PD+TNVQVQ+NTAA G + E
Sbjct: 1 MLQKLIEQAVRSRLIVLIFLIGAMAAAAVMLPKLNLDAFPDVTNVQVQVNTAAEGLAAEE 60
Query: 61 VEQRITYPLETVMAGLPKLEQTRSLSRYGLSQITVIFEEGTDIYFARQLVNERLGGAKDQ 120
VE+ I+YP+E+ M LP + + RSLSR GLS +TV+FEEGTDIYFARQ V E+L A++
Sbjct: 61 VEKLISYPVESAMYALPSVTEVRSLSRTGLSIVTVVFEEGTDIYFARQQVFEQLQAAREA 120
Query: 121 LPDGV-TPTLGPISTGLGEIYFWTVEAEEGATKSDGTPYTPADLREIQDWIIKPQVRNVP 179
+PDGV P +GP ++GLG++Y + + A + + P +LR + D+++K + V
Sbjct: 121 IPDGVGVPEIGPNTSGLGQVYQYILRA------APESGIAPDELRSLNDYLVKLIMMPVG 174
Query: 180 GVTEINTIGGYAKEYQIAPNPDTLRSFGLTLQDLIEAVEQNNNNLGAGYIEKRGEQYLVR 239
GVT++ + GG ++YQ+ +P L S+GL L D+ A+E NN N G ++ K EQ +VR
Sbjct: 175 GVTDVLSFGGEVRQYQVQVDPAKLLSYGLKLDDVTRALEGNNRNAGGWFMAKGQEQLVVR 234
Query: 240 APGQMQSVEDIRDTL----ISNVDGTPVRIRDVATVEVGKELRTGAATENGR-------- 287
G + + E D + ++ GTPVRI DVA V G E+R GA T R
Sbjct: 235 GYGLLPAGEAGLDAIAQIPLTEWQGTPVRIMDVAQVAFGSEIRVGAVTMTRRDEAGSPQV 294
Query: 288 --EVVLGTAFMLIGENSRVVSRAVDDKMKEINLSLPEGVKAITVYDRTVLVDKAISTVKK 345
EVV G +G N++ ++ + I +LP+GV YD++ LV KA++TV+
Sbjct: 295 LGEVVAGVILKRMGANTKATIDDINSRTALIQQALPDGVTFEVFYDQSDLVTKAVTTVRD 354
Query: 346 NLTEGAILVVVILFLFLGNIRAAILTALVIPLSMLFTFTGMVANQVSANLMSLG--ALDF 403
L + +V +L LFL N+RA L L IP+S+ M +SANLMSLG A+
Sbjct: 355 ALLLAFMFIVAVLALFLVNVRATALVLLSIPVSIALALMVMSYFGMSANLMSLGGLAVAI 414
Query: 404 GIIIDGAVVIVENCVRRLAHAQSHHGRALTLSERLHE---------------VFAAAKEV 448
G+++DG+VV+VEN R L + L L E + AA+EV
Sbjct: 415 GMLVDGSVVMVENIFRHLTQPEGRVDNGLPTDPGLSEEDAHREGGYGGIAMRILQAAREV 474
Query: 449 RRPLLYGQLIIMIVYLPIFALTGVEGKMFTPMAFTVVTALFGAIILSVTFVPAAVALFIG 508
P+ + LII++V+ P+FAL GVEGK+F PMA +++ A+ A+++++ VPA
Sbjct: 475 CSPIFFATLIIIVVFAPLFALEGVEGKLFQPMAVSIILAMLSALMVALVVVPALAVYLFR 534
Query: 509 KRVTEKENFLIRNAKRAYAPALDAVMANKPAVLTFAVVVVILSGLVGSRMGSEFVPSLNE 568
+ V +++ L+ + Y L AVMA V A+V+ LS + R+G+EFVP L E
Sbjct: 535 RGVHLRQSPLLTPIDKGYRRVLGAVMARPKTVGITALVLFGLSMALLPRLGTEFVPELEE 594
Query: 569 GDFAIQALRVPATSLSQSVEMQQQLERKLMDEFPEIERIFARTGTAEVASDAMPPNISDG 628
G ++ P SL S+ + +LE LM EFPE++ +R G E+ D P + +
Sbjct: 595 GTINLRVTLAPTASLDTSLMVAPKLEAMLM-EFPEVDYALSRIGAPELGGDPEPVSNIEI 653
Query: 629 YVMLKPQEQWPDPGKSRNQLLSEVQASAAELPGNNYEFSQPIQLRFNELISGVRAAVAVK 688
Y+ LKP ++W SR +L ++ + PG + FSQPI R +EL+SGV+A +A+K
Sbjct: 654 YIGLKPIDEWQSAA-SRAELQRLMEEKLSVFPGLLFTFSQPIATRVDELLSGVKAQLAIK 712
Query: 689 IYGDDMDVLNSTAAEVSEVLGQVPGASEVTVEQTTGLPMLTIDIDRDQIARYGLSLDTVQ 748
++G D+DVL ++SE++ +VPGA +V++EQ +G L + DR ++ARYG+S+D V
Sbjct: 713 LFGPDLDVLAEKGQQLSELVSKVPGAVDVSLEQVSGEAQLVVRPDRARLARYGISVDEVM 772
Query: 749 QAVAVAIGGREAGTLFQGDRRFDIVVRLPDEIRSDLAAIERLPIALPRELNSTISYIPLG 808
V+ IGG +AG + G+ R+DI VRL + R+ AI L + + + + + LG
Sbjct: 773 NLVSTGIGGSDAGQVIDGNARYDINVRLKADTRASADAIADLLL-----VGANGARVRLG 827
Query: 809 EVATLDLAPGPNQISREEGKRRIVVSANVRGRDIGSFVSEAEQKIQAQVDIPAGYWIDWG 868
EVA + + P I R++ +RR+VV ANV GRD+GS V + + + D+P GY + G
Sbjct: 828 EVADVVVEMAPPNIRRDDVQRRVVVQANVSGRDMGSVVKDIYALVP-EADLPPGYTVSVG 886
Query: 869 GTFEQLESATKRLQIVVPVALLLVFILLFMMFNNVKDGLLVFTGIPFALTGGIVALWLRD 928
G +E + A RL +VVPV++ L+ +LL+ F +K L+ +P AL GG+VAL++
Sbjct: 887 GQYENQQRAQARLMLVVPVSIGLIALLLYFSFGALKQVGLIMANVPLALIGGVVALYVSG 946
Query: 929 IPLSISAGVGFIALSGVAVLNGLVMISFIRSLREQGLPLDTAIREGALTRLRPVLMTALV 988
LS+ + +GFI L GVAVLNG+V++ I R G L A+ EG RLRPVLMTAL
Sbjct: 947 TYLSVPSSIGFITLFGVAVLNGVVLVDSINQRRAGGEGLYQAVYEGTAARLRPVLMTALT 1006
Query: 989 ASLGFVPMALNVGTGAEVQRPLATVVIGGILSSTVLTLLVLPLLYQMAHRRED-ELEE 1045
++LG +P+ L+ G G+E+Q+PLA V+IGG+ SST LTLLVLP LY +R+ D ELEE
Sbjct: 1007 SALGLIPILLSSGVGSEIQQPLAVVIIGGLFSSTALTLLVLPTLYMALYRQNDRELEE 1064
Score = 54.7 bits (130), Expect = 4e-11
Identities = 123/560 (21%), Positives = 235/560 (41%), Gaps = 62/560 (11%)
Query: 539 AVLTFAVVVVILSGLVGSRMGSEFVPSLNEGDFA-IQALRVPATSLSQSVEMQQQLERKL 597
AV + +V++ L G + + + +P LN F + ++V + ++ + + E +
Sbjct: 9 AVRSRLIVLIFLIGAMAA--AAVMLPKLNLDAFPDVTNVQVQVNTAAEGLAAE---EVEK 63
Query: 598 MDEFPEIERIFARTGTAEVASDAMPPNISDGYVMLKPQEQWPDPGKSRNQLLSEVQASAA 657
+ +P ++A EV S + +S V+ E+ D +R Q+ ++QA+
Sbjct: 64 LISYPVESAMYALPSVTEVRSLSRT-GLSIVTVVF---EEGTDIYFARQQVFEQLQAARE 119
Query: 658 ELP-GNNYEFSQPIQLRFNELISGV-RAAVAVKIYGDDMDVLNSTAAEVSEVLGQVPGAS 715
+P G P ++ + RAA I D++ LN ++ ++ V G +
Sbjct: 120 AIPDGVGVPEIGPNTSGLGQVYQYILRAAPESGIAPDELRSLNDYLVKL--IMMPVGGVT 177
Query: 716 EVTVEQTTGLPMLTIDIDRDQIARYGLSLDTVQQAVAVAIGGREAGTLFQGDRRFDIVVR 775
+V + + + +D ++ YGL LD V +A+ R AG F + +VVR
Sbjct: 178 DV-LSFGGEVRQYQVQVDPAKLLSYGLKLDDVTRALEG--NNRNAGGWFMAKGQEQLVVR 234
Query: 776 ---LPDEIRSDLAAIERLPIALPRELNSTISYIPLGEVAT-LDLAPGPNQISR--EEGKR 829
L + L AI ++P L + + + + +VA ++ G ++R E G
Sbjct: 235 GYGLLPAGEAGLDAIAQIP--LTEWQGTPVRIMDVAQVAFGSEIRVGAVTMTRRDEAGSP 292
Query: 830 RIV--VSANVRGRDIGSFVSEAEQKIQAQVDIPAGYWIDWGGTFE----QLESATKRLQI 883
+++ V A V + +G+ I ++ + D G TFE Q + TK +
Sbjct: 293 QVLGEVVAGVILKRMGANTKATIDDINSRTALIQQALPD-GVTFEVFYDQSDLVTKAVTT 351
Query: 884 VVPVALLLVFI----LLFMMFNNVKDGLLVFTGIPFALTGGIVALWLRDIPLSISAGVGF 939
V ALLL F+ +L + NV+ LV IP ++ ++ + + ++ + G
Sbjct: 352 VRD-ALLLAFMFIVAVLALFLVNVRATALVLLSIPVSIALALMVMSYFGMSANLMSLGGL 410
Query: 940 IALSGVAVLNGLVMISFI-RSLRE------QGLPLDTAIRE----------GALTRL--- 979
G+ V +VM+ I R L + GLP D + E G R+
Sbjct: 411 AVAIGMLVDGSVVMVENIFRHLTQPEGRVDNGLPTDPGLSEEDAHREGGYGGIAMRILQA 470
Query: 980 -----RPVLMTALVASLGFVPMALNVGTGAEVQRPLATVVIGGILSSTVLTLLVLPLLYQ 1034
P+ L+ + F P+ G ++ +P+A +I +LS+ ++ L+V+P L
Sbjct: 471 AREVCSPIFFATLIIIVVFAPLFALEGVEGKLFQPMAVSIILAMLSALMVALVVVPALAV 530
Query: 1035 MAHRREDELEEQEIALAADR 1054
RR L + + D+
Sbjct: 531 YLFRRGVHLRQSPLLTPIDK 550