Pairwise Alignments

Query, 592 a.a., PQQ-dependent dehydrogenase, methanol/ethanol family from Pseudomonas stutzeri RCH2

Subject, 379 a.a., Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8) from Pseudomonas fluorescens FW300-N2E2

 Score =  209 bits (531), Expect = 2e-58
 Identities = 122/327 (37%), Positives = 176/327 (53%), Gaps = 23/327 (7%)

Query: 12  LTALCAAVAMSSLHAWA------VTDQEILNDAKSTDQIVTNGMGLQGQRYSTLDALNAN 65
           L +L  A+A+S + AWA      V  + I+   +     ++ G     QRYS L+ ++  
Sbjct: 21  LKSLFVALALSVMPAWALDMPANVDGKRIIAANQEPGNWMSTGRTYDEQRYSPLNKISDR 80

Query: 66  NINQLRPVWGFSLGGEKQRGQEAQPLIKDGVMYITGSYSRVYAVDARTGKELWQYDARLP 125
           N++QL   W + L  +  RG EA P++ DGVMY TG +S VYA+DAR G+ +W+YD +  
Sbjct: 81  NVSQLGLAWSYKL--DLDRGVEATPIVVDGVMYTTGPFSVVYALDARNGRLIWKYDPQSD 138

Query: 126 DGIM--PCCDVINRGVALYDDLVIFGTLDAKLVALNKDTGKVVWK-KTVADYKAGYSLTA 182
                  CCD +NRGVA++   V  G LD +L A++  TG+  W   T AD+   Y++T 
Sbjct: 139 RNRAGEACCDAVNRGVAVWRGKVYVGVLDGRLEAIDARTGQRAWSVDTRADHSRSYTITG 198

Query: 183 APLIVKGKLITGVSGGEFGVVGKIEAYNPKNGELLWTRPTVEGHMGYVWKDGKKVESGIS 242
           AP +V GK++ G  G  FGV G + AY+ + G+  W   TV G       D K      +
Sbjct: 199 APRVVNGKVVIGNGGAAFGVRGYVTAYDAETGKQAWRFYTVPG-------DPKLPPEDKA 251

Query: 243 GGEAGKTWPGDLW--KTGGAAPWLGGYYDPDTDSLLFGTGNPAPWNSHLR---PGDNLYS 297
              A KTW GD +  + GG   W    +DPD + L  G GN + W+   R    GDNL+ 
Sbjct: 252 MAIAAKTWHGDAFVEQGGGGTAWDSFAFDPDLNLLYIGVGNGSLWDPKWRSQAKGDNLFL 311

Query: 298 SSRLALDPNDGSIKWHFQTTPHDGWDY 324
           SS +A++ + G   WH+QTTP D WDY
Sbjct: 312 SSIVAVNADTGEYVWHYQTTPGDAWDY 338



 Score = 40.0 bits (92), Expect = 2e-07
 Identities = 40/142 (28%), Positives = 55/142 (38%), Gaps = 26/142 (18%)

Query: 435 SNEWSMDIWNEGTAYKKGAAYLGAGFTIHPLNDDYIGVLRAIDPKTGKEVWRYKNYAP-- 492
           + E   D  N G A  +G  Y+G             G L AID +TG+  W     A   
Sbjct: 142 AGEACCDAVNRGVAVWRGKVYVGV----------LDGRLEAIDARTGQRAWSVDTRADHS 191

Query: 493 ----LWGGVLATKGNLVFTGNP------EGYLMAFDAKTGKKLYEFNTGSGVIGSPVTWE 542
               + G      G +V  GN        GY+ A+DA+TGK+ + F T   V G P    
Sbjct: 192 RSYTITGAPRVVNGKVVI-GNGGAAFGVRGYVTAYDAETGKQAWRFYT---VPGDPKLPP 247

Query: 543 MDGEQYVSVLSGWGGAVPLWGG 564
            D    ++  +  G A    GG
Sbjct: 248 EDKAMAIAAKTWHGDAFVEQGG 269



 Score = 31.6 bits (70), Expect = 6e-05
 Identities = 40/150 (26%), Positives = 64/150 (42%), Gaps = 24/150 (16%)

Query: 400 AGAQGKTVTVAPSFLGGKNWMPMAYSQDTGLFYVPSNEWS-MDIWNEGTAYKKGAAYLGA 458
           A   GK +  A    G  NWM    + D    Y P N+ S  ++   G A+      L  
Sbjct: 42  ANVDGKRIIAANQEPG--NWMSTGRTYDEQR-YSPLNKISDRNVSQLGLAWSYKLD-LDR 97

Query: 459 GFTIHPLNDDYI-------GVLRAIDPKTGKEVWRY-----KNYA------PLWGGVLAT 500
           G    P+  D +        V+ A+D + G+ +W+Y     +N A       +  GV   
Sbjct: 98  GVEATPIVVDGVMYTTGPFSVVYALDARNGRLIWKYDPQSDRNRAGEACCDAVNRGVAVW 157

Query: 501 KGNLVFTGNPEGYLMAFDAKTGKKLYEFNT 530
           +G  V+ G  +G L A DA+TG++ +  +T
Sbjct: 158 RGK-VYVGVLDGRLEAIDARTGQRAWSVDT 186



 Score = 24.3 bits (51), Expect = 0.010
 Identities = 17/75 (22%), Positives = 32/75 (42%), Gaps = 16/75 (21%)

Query: 415 GGKNWMPMAYSQDTGLFYVPSNEWSMDIWN-EGTAYKKGAAYLGAGFTIHPLNDDYIGVL 473
           GG  W   A+  D  L Y+     S  +W+ +  +  KG             ++ ++  +
Sbjct: 270 GGTAWDSFAFDPDLNLLYIGVGNGS--LWDPKWRSQAKG-------------DNLFLSSI 314

Query: 474 RAIDPKTGKEVWRYK 488
            A++  TG+ VW Y+
Sbjct: 315 VAVNADTGEYVWHYQ 329