Pairwise Alignments
Query, 1613 a.a., NAD-glutamate dehydrogenase from Vibrio cholerae E7946 ATCC 55056
Subject, 1620 a.a., NAD-specific glutamate dehydrogenase from Pseudomonas aeruginosa PA14
Score = 1446 bits (3742), Expect = 0.0
Identities = 753/1584 (47%), Positives = 1032/1584 (65%), Gaps = 17/1584 (1%)
Query: 29 PLVTQLGQHLFSNISQDDLVERNESDLYGAVLSLWHHINEKKADERSVRVFNPTVSRQGW 88
P VT + FS IS D+L +R SDL G LS W + D+ VRV+NP + GW
Sbjct: 30 PQVTLFAEQFFSLISLDELTQRRLSDLVGCTLSAWRLLERFDRDQPEVRVYNPDYEKHGW 89
Query: 89 QSTHTIVEIVLPDSPFLVDSIKMALSRLGLASHLMLNGPAHIARHDDGSIKSI----NQG 144
QSTHT VE++ PD PFLVDS++M L+R G + H + + R G +K I +QG
Sbjct: 90 QSTHTAVEVLHPDLPFLVDSVRMELNRRGYSIHTLQTNVLSVRRSAKGELKEILPKGSQG 149
Query: 145 EG-QLTSMFHIEVDRLSSKEEMTELKNELLDILHDTALVVKDWKPMATKLEQVINQLEAD 203
+ S+ ++E+DR + E+ L+ +L++L + + V D++PM K +++ L
Sbjct: 150 KDVSQESLMYLEIDRCAHAGELRALEKAILEVLGEVRVTVADFEPMKAKARELLTWLGKA 209
Query: 204 KKQIPVEAERLQETIQFLRWLGNHNFTFMGYKEFDLVEKNGDTELTPTKDTGLGLFSDNE 263
K ++P AE L+E +L WL +++FTF+GY+EF + ++ + + + LGL
Sbjct: 210 KLKVP--AEELKEVRSYLEWLLDNHFTFLGYEEFSVADEADGGRMVYDEKSFLGLTRLLR 267
Query: 264 RVRSVKLSQFPDSARLEAKKPFLLILTKGNKQSRIHRPAYTDYIGIKKFDAKGKVIGEHR 323
S D A ++P LL K SR+HRPAY DY+ I++ D KG+VI E R
Sbjct: 268 AGLSKDDLHIEDYAVAYLREPVLLSFAKAAHPSRVHRPAYPDYVSIRELDGKGRVIRECR 327
Query: 324 FTGLYTSAVYNQSVEGIPLIREKVGRILAASGYRQGSYAYKALHNILENYPRDELLQARE 383
F GL+TS+VYN+SV IP IR KV ++ SG+ ++ K L +LE PRD+L Q
Sbjct: 328 FMGLFTSSVYNESVNDIPFIRGKVAEVMRRSGFDTKAHLGKELAQVLEVLPRDDLFQTPV 387
Query: 384 EELLEVGMGVVQMQDRDLLRLFVRKDPFGRFFSCMVYVTKERYNTELRRKTQQVFKQYFG 443
+EL + +V++Q+R+ +R+F+RKDP+GRF C+ YV ++ Y+TE R K QQV +
Sbjct: 388 DELFSTALAIVRIQERNKIRVFLRKDPYGRFCYCLAYVPRDVYSTETRLKIQQVLMERLQ 447
Query: 444 CEQDVEFTTYFSESPLARTHYIVRVD-NNNINVDVKKIEQNLMEASTSWDDRLAEAIVAN 502
D EF T+FSES LAR +I+RVD + I++D ++E+ +++A SW D + +V N
Sbjct: 448 AS-DCEFWTFFSESVLARVQFILRVDPKSRIDIDPARLEEEVIQACRSWQDDYSSLVVEN 506
Query: 503 FGESRGLPLSKEYQRAFPRSYKEDVMPGSALADIEHLEALDEHNKLGMLFYRLQETAKDS 562
GE++G + ++ + FP Y+E P A+ D++HL +L E L M FY Q A+
Sbjct: 507 LGEAKGTNVLADFPKGFPAGYRERFAPHFAVVDLQHLLSLSEQRPLVMSFY--QPLAQGE 564
Query: 563 KAVRLKLYHKDEPIHLSDVMPMLENLGLRVIGESPYEVVKANGQVYWILDFSMLHKSDKQ 622
+ + KLYH D P+ LSDV+P+LENLGLRV+GE PY + NG+ YWI DF+ +
Sbjct: 565 QQLHCKLYHADTPLALSDVLPILENLGLRVLGEFPYRLRHQNGREYWIHDFAFTYAEGLD 624
Query: 623 VDLREARDRFQQAFAAIWAGELESDGFNRLILGASLSGREVSILRAYARYMRQVGFPFSQ 682
VD+++ + Q AF I +G+ E+D FNRL+L A+L R+V++LRAYARY++Q+ F
Sbjct: 625 VDIQQLNEILQDAFVHIVSGDAENDAFNRLVLTANLPWRDVALLRAYARYLKQIRLGFDL 684
Query: 683 HYIEDTLSHHPDLAQGLVDLFVRRFDPKYK----GGEKGQAEIIKSLTEQLDQVQSLDDD 738
YI L+ H D+A+ LV LF RF K E Q ++ +++ LD+VQ L++D
Sbjct: 685 GYIASALNAHTDIARELVRLFKTRFYLARKLTAEDLEDKQQKLEQAILGALDEVQVLNED 744
Query: 739 RIIRRYMEMINATLRTNYYQLDEHKQNKPWLSLKMKPSEIPEIPAPVPAFEIFVYAPDIE 798
RI+RRY+++I ATLRTN+YQ D + QNK + S K P IPE+P PVP +EIFVY+P +E
Sbjct: 745 RILRRYLDLIKATLRTNFYQPDGNGQNKSYFSFKFNPKAIPELPRPVPKYEIFVYSPRVE 804
Query: 799 GVHLRGGKVARGGLRWSDRQEDFRTEILGLVKAQQVKNTVIVPVGAKGGFVCKKQYLYTT 858
GVHLRGGKVARGGLRWSDR+EDFRTE+LGLVKAQQVKN VIVPVGAKGGFV ++ L +
Sbjct: 805 GVHLRGGKVARGGLRWSDREEDFRTEVLGLVKAQQVKNAVIVPVGAKGGFVPRRLPLGGS 864
Query: 859 RDEIFAEGQRCYKRFIRALLDVTDNIIEGQVVPPKNVVRHDEDDPYLVVAADKGTATFSD 918
RDEI AE CY+ FI LLD+TDN+ EG+VVPP NVVRHDEDDPYLVVAADKGTATFSD
Sbjct: 865 RDEIQAEAIACYRIFISGLLDITDNLKEGEVVPPANVVRHDEDDPYLVVAADKGTATFSD 924
Query: 919 LANSVSAEYQFWLGDAFASGGSNGYDHKAMGITAKGGWESVKRHFREMGIDCQTTDFTAI 978
+AN ++AEY FWLGDAFASGGS GYDHK MGITAKG W SV+RHFRE GID Q + + I
Sbjct: 925 IANGIAAEYGFWLGDAFASGGSAGYDHKGMGITAKGAWVSVQRHFRERGIDVQKDNISVI 984
Query: 979 GIGDMAGDVFGNGMLLSKHIRLLAAFNHIHIFIDPTPDSASSWEERNRLFNLPRSSWEDY 1038
GIGDMAGDVFGNG+L+S ++L+AAFNH+HIFIDP PD+ASS+ ER RLFNLPRSSW DY
Sbjct: 985 GIGDMAGDVFGNGLLMSDKLQLVAAFNHMHIFIDPNPDAASSFVERQRLFNLPRSSWADY 1044
Query: 1039 NPKLISKGGGVFSRKAKAITLTPEMQKMLNTKKTTLAPNELIKMILKMEVDLLWNGGIGT 1098
+ KLIS GGG+F R AK+I +TPEMQ + + LAP ELI +LK VDLLWNGGIGT
Sbjct: 1045 DAKLISAGGGIFLRSAKSIAITPEMQARFDIQADRLAPTELIHALLKAPVDLLWNGGIGT 1104
Query: 1099 YVKSSIETHTDVGDRANDGLRVDGREVNAKIIGEGGNLGMTQRGRIEFALKGGRVNTDFV 1158
YVKSS ETH DVGD+ANDGLRVDGRE+ AK++GEGGNLGMTQ R+EF L GG NTDF+
Sbjct: 1105 YVKSSKETHADVGDKANDGLRVDGRELRAKVVGEGGNLGMTQLARVEFGLHGGANNTDFI 1164
Query: 1159 DNVGGVDCSDNEVNIKIFLNGLVANGDLTLKQRNQILESMKDEVGSIVIEDAYGQSESIS 1218
DNVGGVDCSD+EVNIKI LN +V GD+T KQRN +L M D VG++V+ + Y Q++++S
Sbjct: 1165 DNVGGVDCSDHEVNIKILLNEVVQAGDMTEKQRNALLVKMTDAVGALVLGNNYKQTQALS 1224
Query: 1219 VTEAQGVSLMKEQIRFIHHMEKAGYLDRALEHIPDDETLLERERQGMGLTRPELSVLMAY 1278
+ + + + E R + +E G LDRALE +P DE L ER G GLTR ELSVL++Y
Sbjct: 1225 LAQRRARERIAEYKRLMGDLEARGKLDRALEFLPSDEELAERISAGQGLTRAELSVLISY 1284
Query: 1279 GKMALKEELASEEIAQDEFHAKQLVNYFPTELRGHYAQQMVNHPLRVEIIATALANQMVN 1338
K+ LKE L + D++ + + FP L + M H L+ EI++T +AN +VN
Sbjct: 1285 SKIDLKESLLKSLVPDDDYLTRDMETAFPALLAEKFGDAMRRHRLKREIVSTQIANDLVN 1344
Query: 1339 EMGCNFVTRLQEETGSSVVDIANAYAAAREIYGLGIVLEKVRKLDNIAQSSAQYDVMFLV 1398
MG FV RL+E TG S ++A AY R+++ L ++ LD + Q +M +
Sbjct: 1345 HMGITFVQRLKESTGMSAANVAGAYVIVRDVFHLPHWFRQIENLDYQVPADIQLTLMDEL 1404
Query: 1399 RRTLRRLTRWLLRNRTGKPSVIAMVERYQEDVKAITEQLDKVLVKEEIVEHNSMAENWIE 1458
R RR TRW LR+R + V + + A+ +L+++L + + +++
Sbjct: 1405 MRLGRRATRWFLRSRRNELDAARDVAHFGPRIAALGLKLNELLEGPTRELWQARYQTYVD 1464
Query: 1459 KGIEKELAHYVARLSSLYSVLDISAVAKEKGIAVTQTAKLYFHLGDRLSLHWFLKQINHQ 1518
G+ + LA VA S LY++L I + G + AK YF +G L L W+L+QI +
Sbjct: 1465 AGVPELLARMVAGTSHLYTLLPIIEASDVTGQDTAEVAKAYFAVGSALDLTWYLQQITNL 1524
Query: 1519 AVDNHWQALARASFREDLDWQQRQLTAQVLSSNLSDAQQEIELALDKWLERNQVSISRWE 1578
V+N+WQALAR +FR+DLDWQQR +T VL + D +E+E + WLE++ + RW
Sbjct: 1525 PVENNWQALAREAFRDDLDWQQRAITVSVL--QMQDGPKEVEARVGLWLEQHLPLVERWR 1582
Query: 1579 NILSEFKVGTVHEFAKFSVALREL 1602
+L E + + ++A ++VA REL
Sbjct: 1583 AMLVELRAASGTDYAMYAVANREL 1606