Pairwise Alignments

Query, 949 a.a., isoleucine--tRNA ligase from Vibrio cholerae E7946 ATCC 55056

Subject, 948 a.a., valine--tRNA ligase from Pseudomonas fluorescens FW300-N2C3

 Score =  169 bits (428), Expect = 8e-46
 Identities = 201/912 (22%), Positives = 354/912 (38%), Gaps = 171/912 (18%)

Query: 50  SFVLHDGPPYANGDIHIGHALNKILKDVIIKSKTLSGFDAPYIPGWDCHGLPIELMVEKK 109
           S+ +   PP   G +H+GH  N  + D +I+ + + G +  + PG D  G+  +++VE++
Sbjct: 32  SYTIMIPPPNVTGSLHMGHGFNNAIMDALIRFRRMQGRNTLWQPGTDHAGIATQMLVERR 91

Query: 110 VGKPGQK---VTAAEFREKCREYAAGQVEGQKESFKRLGILGEWDKPYRTMDFVTEANII 166
           +   GQ    +   +F EK  E+            +RLG   +W +   TMD      + 
Sbjct: 92  LEAQGQNRHDLGREKFLEKVWEWKDESGGNISRQIRRLGSSVDWSRERFTMDDGLSEAVK 151

Query: 167 RALGKIADNGHLLKGFKPVHWCTDCGSALAEAEVEYKNKVSPSIDVRFKAADEAAVLAKF 226
            A  ++ ++G + +G + V+W T   +A+++ EVE  ++     ++++  AD A      
Sbjct: 152 EAFVRLHEDGLIYRGKRLVNWDTKLHTAISDLEVENHDEKGFLWNLKYPLADGAKTA--- 208

Query: 227 GLAAGHEGKGDVSIVIWTTTPWTLPANRAVCLRADLEYVLIQVEGEQPERIIVASELAKS 286
                 EGK    +++ TT P T+  + AV +  +          E+ + +I        
Sbjct: 209 ------EGKD--YLIVATTRPETMLGDAAVAVNPN---------DERYQALI-------- 243

Query: 287 VMDRAGIEHFHNLGFATGADLELVQFQHPFYSFTVPAILGDHVTTDSGTGVVHTAPGHGQ 346
                            G  +EL     P     +P I  D+   + GTG V   P H  
Sbjct: 244 -----------------GKFVEL-----PLVGRRIPIIGDDYCDPEFGTGCVKITPAHDF 281

Query: 347 EDFAVGQQYGLEVANPVGSNGVYLPDTELF------------------AGQHVFKANDSV 388
            D+ VG+++ L + N    N   LP  ++F                  AG   F+A   +
Sbjct: 282 NDYEVGKRHNLPLLNIFDKNANVLPAAQVFNLDGTLNENVDGTLPAEYAGLDRFEARKQI 341

Query: 389 LEVLKEKGALLHHHAYEHSYPHCWRHKTPIIFRATPQWFVSMEQAGLREQALTAIKG--V 446
           +   +  G L+    +    P   R  T I    T QW+VS +   L E A+ A++   +
Sbjct: 342 VAAFEAAGLLVSVDDHALKVPKGDRSGTIIEPWLTDQWYVSTKP--LAEPAIAAVEDGRI 399

Query: 447 HWMPDWGQSRIEGMVAGRPEWCISRQRTWGVPIALFVHKETAELHPNSADLIEKVAQLVE 506
            ++P   ++     +    +WCISRQ  WG  I  + + E+ +++    +          
Sbjct: 400 QFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAW-YDESGKVYVGRDEA--------- 449

Query: 507 QKGIQAWWDLDTAELLGAEDAANYEKVLDTLDVWFDSGVTHSAVVDARQEFNGAE----A 562
                   ++     LG + A   +   D LD WF SG+   + +   Q+    +     
Sbjct: 450 --------EVRAKHNLGPDVALTQDN--DVLDTWFSSGLWTFSTLGWPQQTEFLKKFHST 499

Query: 563 DMYLEGSDQHRGWFQSSLISSV-AMKG-----KAPYKEVLTHGFVVDGQGRKMSKSIGNV 616
           D+ + G D    W    ++ ++  MK      + P+K V  HG V DGQG+KMSKS GNV
Sbjct: 500 DVLVTGFDIIFFWVARMIMMTMHLMKNEDGTPQVPFKTVYVHGLVRDGQGQKMSKSKGNV 559

Query: 617 VAPQDVTNKL-------------------------------------GADILRLWVASTD 639
           + P D+ + +                                     G D LR    S  
Sbjct: 560 LDPLDIIDGIDLETLVQKRTSGLMQPKLAKKIEKATREEFAEGIASYGTDALRFTFCSLA 619

Query: 640 YTGEVAVSDEILKRSADAYR----RIRNTARFFLANLNGFNPTTDIIPVEDMVALDRWAV 695
            TG     D       + YR    +I N AR+ L          +     ++   DRW +
Sbjct: 620 STGRDIKFD---MGRVEGYRNFCNKIWNAARYVLDKGEDCGQNGE---AYELTLADRWII 673

Query: 696 GRALAAQQEIIQAYQDYNLHAVVQRLMNFCSIEMGSFYLDVIKDRQYTAKRGGHAQRSCQ 755
            +    + E+ +    +      Q L  F   +   +YL++ K   +        QR  +
Sbjct: 674 SQLQRTEAEVTRQLDQFRFDLAAQALYEFIWNQYCDWYLELSKPVLWDENAPVERQRGTR 733

Query: 756 TALFFIVEALVRWMAPIMSFTADEIWNAMPAQQADGSARDKFVFTTEWFDGLFGLAEGEE 815
             L  ++E  +R   P M F  +EIW  +         + K +    W      +A    
Sbjct: 734 RTLVRVLEVALRLAHPFMPFITEEIWQRIAPL---AGIQGKTIMLQPW-----PVANETR 785

Query: 816 LNNAFWNDIQKVRGSVNKLLENARNEKLIGGSLQAELVLFADDSLASKLAKLGDELRFVL 875
           ++ A  +DI+ ++ +    L N R E  IG      L LF  ++ A  L +L +      
Sbjct: 786 IDPAAEDDIEWLK-TFMLGLRNIRAEMNIGPG--KPLALFLKNASAEDLRRLNE------ 836

Query: 876 LTSKAVVKPLAE 887
             ++A++K LA+
Sbjct: 837 --NEALLKKLAK 846