Pairwise Alignments

Query, 949 a.a., isoleucine--tRNA ligase from Vibrio cholerae E7946 ATCC 55056

Subject, 943 a.a., isoleucine--tRNA ligase from Pseudomonas fluorescens FW300-N2C3

 Score = 1060 bits (2740), Expect = 0.0
 Identities = 525/960 (54%), Positives = 682/960 (71%), Gaps = 28/960 (2%)

Query: 1   MSEYKDTLNLPETGFPMRGDLAKREPEMLQRWYQEDLYGAIRQAKKGKKSFVLHDGPPYA 60
           M++YK TLNLP+T FPM+  L +REP++LQRW    LYG +R+  K +  FVLHDGPPYA
Sbjct: 1   MTDYKATLNLPDTAFPMKAGLPQREPQILQRWDSIGLYGKLREIGKDRPKFVLHDGPPYA 60

Query: 61  NGDIHIGHALNKILKDVIIKSKTLSGFDAPYIPGWDCHGLPIELMVEKKVGKPGQKVTAA 120
           NG IHIGHALNKILKD+II+SKTLSGFDAPY+PGWDCHGLPIE  VE   GK    + A 
Sbjct: 61  NGTIHIGHALNKILKDMIIRSKTLSGFDAPYVPGWDCHGLPIEHKVEVTHGK---NLGAD 117

Query: 121 EFREKCREYAAGQVEGQKESFKRLGILGEWDKPYRTMDFVTEANIIRALGKIADNGHLLK 180
           + RE CR YA  Q+EGQK  F RLG+LG++  PY+TMDF  EA  IRAL KI + G + K
Sbjct: 118 KTRELCRAYATEQIEGQKSEFIRLGVLGDFANPYKTMDFKNEAGEIRALAKIVEGGFVFK 177

Query: 181 GFKPVHWCTDCGSALAEAEVEYKNKVSPSIDVRFKAADEAAVLAKFGLAAGHEGKGDVSI 240
           G KPV+WC DCGSALAEAEVEY+NK S +IDV F  ADE  + A FGL  G   K   SI
Sbjct: 178 GLKPVNWCFDCGSALAEAEVEYENKKSSTIDVAFPIADEDKLAAAFGL--GKLAK-PASI 234

Query: 241 VIWTTTPWTLPANRAVCLRADLEYVLIQVEGEQPERIIVASELAKSVMDRAGIEHFHNLG 300
           VIWTTTPWT+PAN+A+ +  +  Y L+ V  +    +++A EL ++ + R  +E    + 
Sbjct: 235 VIWTTTPWTIPANQALNVHPEFTYALVDVGDKL---LVLAEELVEACLARYNLEG-SVVA 290

Query: 301 FATGADLELVQFQHPFYSFTVPAILGDHVTTDSGTGVVHTAPGHGQEDFAVGQQYGL--- 357
            A G+ LEL+ F+HPFY    P  L D+V   +GTGVVH+AP +G +DF   ++YG+   
Sbjct: 291 TAPGSALELINFRHPFYDRLSPVYLADYVELGAGTGVVHSAPAYGVDDFVTCKKYGMVND 350

Query: 358 EVANPVGSNGVYLPDTELFAGQHVFKANDSVLEVLKEKGALLHHHAYEHSYPHCWRHKTP 417
           ++ NPV SNGVY P  E F GQ ++KAN ++++ L E GALLH    EHSY HCWRHKTP
Sbjct: 351 DILNPVQSNGVYAPSLEFFGGQFIWKANPAIVDKLTEVGALLHTTVIEHSYMHCWRHKTP 410

Query: 418 IIFRATPQWFVSMEQAG-----LREQALTAIKGVHWMPDWGQSRIEGMVAGRPEWCISRQ 472
           +I+RAT QWF+ M++       LR+++L AI+   ++P WGQ+R+  M+A RP+WCISRQ
Sbjct: 411 LIYRATAQWFIGMDKQPTSGDTLRQRSLKAIEETQFVPAWGQARLHSMIANRPDWCISRQ 470

Query: 473 RTWGVPIALFVHKETAELHPNSADLIEKVAQLVEQKGIQAWWDLDTAELLGAEDAANYEK 532
           R WGVPI  F++KE+ ELHP + +L+E VA+ VE +GI+AW+ LD AELLG ++A  Y+K
Sbjct: 471 RNWGVPIPFFLNKESGELHPRTVELMEVVAKRVEAEGIEAWFKLDAAELLG-DEAPLYDK 529

Query: 533 VLDTLDVWFDSGVTHSAVVDARQEF---NGAEADMYLEGSDQHRGWFQSSLISSVAMKGK 589
           + DTLDVWFDSG TH  V+         +G  AD+YLEGSDQHRGWF SSL++  A+   
Sbjct: 530 ISDTLDVWFDSGTTHWHVLRGSHPMGHESGPRADLYLEGSDQHRGWFHSSLLTGCAIDNH 589

Query: 590 APYKEVLTHGFVVDGQGRKMSKSIGNVVAPQDVTNKLGADILRLWVASTDYTGEVAVSDE 649
           APY+E+LTHGF VD  GRKMSKS+GNV+APQ V + LGADI+RLWVASTDY+GE+AVS++
Sbjct: 590 APYRELLTHGFTVDESGRKMSKSLGNVIAPQKVNDTLGADIMRLWVASTDYSGEMAVSEQ 649

Query: 650 ILKRSADAYRRIRNTARFFLANLNGFNPTTDIIPVEDMVALDRWAVGRALAAQQEIIQAY 709
           IL+RSADAYRRIRNTARF L+NL GFNP TD++P E+M+ALDRWAV R L  Q+E+ + Y
Sbjct: 650 ILQRSADAYRRIRNTARFLLSNLTGFNPATDLLPAEEMLALDRWAVDRTLLLQRELQEHY 709

Query: 710 QDYNLHAVVQRLMNFCSIEMGSFYLDVIKDRQYTAKRGGHAQRSCQTALFFIVEALVRWM 769
            +Y    V  ++ NFC  E+G FYLD+IKDRQYT      A+RSCQTALF I EALVRW+
Sbjct: 710 GEYRFWNVYSKIHNFCVQELGGFYLDIIKDRQYTTGANSKARRSCQTALFHISEALVRWI 769

Query: 770 APIMSFTADEIWNAMPAQQADGSARDKFVFTTEWFDGLFGLAEGEELNNAFWNDIQKVRG 829
           API++FTADE+W  +P +      R++ V    W++GL  L +G EL+ A+W+ +  V+ 
Sbjct: 770 APILAFTADELWQYLPGE------RNESVMLNTWYEGLTELPQGFELDRAYWDRVMAVKA 823

Query: 830 SVNKLLENARNEKLIGGSLQAELVLFADDSLASKLAKLGDELRFVLLTSKAVVKPLAEKS 889
           +VNK +E  R  K +GG+LQAE+ L+A+++L + LAKLG+ELRFVL+TS A V P  +  
Sbjct: 824 AVNKEMEIQRAAKAVGGNLQAEVTLYAEEALGADLAKLGNELRFVLITSTASVAPFVQAP 883

Query: 890 EAAQATDIDGLFVQVNKTEAEKCDRCWHHTPDVGTIAGHTTICGRCVSNVEGEGEVRKFA 949
             A  T++ GL +++ K+   KC RCWH   DVG    H  ICGRCV N+ G GEVR +A
Sbjct: 884 ADAVTTEVSGLKLKIVKSNHTKCARCWHCREDVGVNPEHPEICGRCVDNISGAGEVRHYA 943