Pairwise Alignments

Query, 949 a.a., isoleucine--tRNA ligase from Vibrio cholerae E7946 ATCC 55056

Subject, 959 a.a., valyl-tRNA synthetase (RefSeq) from Shewanella loihica PV-4

 Score =  150 bits (378), Expect = 5e-40
 Identities = 188/840 (22%), Positives = 324/840 (38%), Gaps = 165/840 (19%)

Query: 25  EPEMLQRWYQEDLYGAIRQAKKGKKSFVLHDGPPYANGDIHIGHALNKILKDVIIKSKTL 84
           E  + Q W ++  +        G    ++   PP   G +H+GHA    + D +I+ + +
Sbjct: 11  EQALYQNWEEKGYFKPHGDESNGNYCIMIP--PPNVTGSLHMGHAFQDTIMDTLIRYQRM 68

Query: 85  SGFDAPYIPGWDCHGLPIELMVEKKV----GKPGQKVTAAEFREKCREYAAGQVEGQKES 140
            G +  +  G D  G+  +++VE+KV    GK    +    F ++  ++         + 
Sbjct: 69  KGKNTLWQVGTDHAGIATQMLVERKVEAEEGKSRHDLGRETFIDRIWDWKNQSGGTITKQ 128

Query: 141 FKRLGILGEWDKPYRTMDFVTEANIIRALGKIADNGHLLKGFKPVHWCTDCGSALAEAEV 200
            +RLG   +WD+   TMD    A +     ++ ++  + +G + V+W     +A+++ EV
Sbjct: 129 LRRLGASVDWDRERFTMDEGMSAAVQEVFVRLYNDDLIYRGKRLVNWDPKLHTAISDLEV 188

Query: 201 EYKNKVSPSIDVRFKAADEAAVLAKFGLAAGHEGKGDVSIVIWTTTPWTLPANRAVCLRA 260
           E K K       R+  AD A            +GK  + +   TT P T+  + AV +  
Sbjct: 189 ENKEKQGSMWHFRYPLADGALTA---------DGKDYLEVA--TTRPETMLGDSAVAVHP 237

Query: 261 DLEYVLIQVEGEQPERIIVASELAKSVMDRAGIEHFHNLGFATGADLELVQFQHPFYSFT 320
           D E                 S + K ++                          P  +  
Sbjct: 238 DDERY--------------QSLIGKFIL-------------------------LPIVNRR 258

Query: 321 VPAILGDHVTTDSGTGVVHTAPGHGQEDFAVGQQYGL----------------EVANPVG 364
           +P +  D+V  + GTG V   P H   D+ VG+++ L                EV N  G
Sbjct: 259 IPIVADDYVDMEFGTGCVKITPAHDFNDYEVGKRHNLPMFNILTIDAAIRSQAEVVNSDG 318

Query: 365 SNGVYLPDT--ELFAGQHVFKANDSVLEVLKEKGALLHHHAYEHSYPHCWRHKTPIIFRA 422
           +    L  +  E FAG   FKA  ++++  +  G L     +    P+  R    I    
Sbjct: 319 TANDELDGSLPERFAGLDRFKARTAIVDEFESLGLLGKIDPHALKVPYGDRSGVVIEPLL 378

Query: 423 TPQWFVSMEQAGLREQALTAIKG--VHWMPDWGQSRIEGMVAGRPEWCISRQRTWGVPIA 480
           T QW+V++  A + + A+ A++   + ++P   ++     +    +WCISRQ  WG  I 
Sbjct: 379 TDQWYVAV--APMAKTAIEAVENGDIKFVPQQYENMYFSWMRDIQDWCISRQLWWGHRI- 435

Query: 481 LFVHKETAELHPNSADLIEKVAQLVEQKGIQAWWDLDTAELLGAEDAANYEKVLDTLDVW 540
                      P   D   KV    ++  ++A  +LD + +L  +         D LD W
Sbjct: 436 -----------PAWYDEAGKVYVGRDEAEVRAKHNLDDSVVLRQDP--------DVLDTW 476

Query: 541 FDSGV-THSAV-----VDARQEFNGAEADMYLEGSDQHRGWFQSSLISSVAM------KG 588
           F S + T S +      +A + F+    D+ + G D    W    ++ ++        K 
Sbjct: 477 FSSALWTFSTLGWPDDTEALKTFH--PTDVLVTGFDIIFFWVARMIMMTMHFIKDEDGKP 534

Query: 589 KAPYKEVLTHGFVVDGQGRKMSKSIGNVVAPQDVTNKL---------------------- 626
           + P+K V   G + D QG KMSKS GNV+ P D+ + +                      
Sbjct: 535 QVPFKTVYVTGLIRDEQGNKMSKSKGNVLDPLDMIDGIDLEALVEKRTGNMMQPQLAAKI 594

Query: 627 ---------------GADILRLWVASTDYTGEVAVSDEILKRSADAYR----RIRNTARF 667
                          G D LR  +A+   TG     D  +KR  D YR    +I N +R+
Sbjct: 595 EKSTRKEFADGIEAHGTDALRFTLAAMASTGRDINWD--MKR-LDGYRSFCNKIWNASRY 651

Query: 668 FLANLNGFN-----PTTDIIPVEDMVALDRWAVGRALAAQQEIIQAYQDYNLHAVVQRLM 722
            L N    +     P         +   DRW VG      +   +  ++Y        L 
Sbjct: 652 VLMNTEEQDCGPQSPNGKADGEMQLSLADRWIVGLFNQTVKAFDEHMENYRFDLAANTLY 711

Query: 723 NFCSIEMGSFYLDVIKDRQYTAKRGGHA-QRSCQTALFFIVEALVRWMAPIMSFTADEIW 781
            F   +   +YL++ K      + G  A QR  +  L  ++EA+ R + P+M +  + IW
Sbjct: 712 EFTWNQFCDWYLELTKP---VLQNGTEAEQRGTRHTLVTVLEAMQRLLHPMMPYLTETIW 768