Pairwise Alignments

Query, 949 a.a., isoleucine--tRNA ligase from Vibrio cholerae E7946 ATCC 55056

Subject, 886 a.a., valine--tRNA ligase from Methanococcus maripaludis JJ

 Score =  223 bits (569), Expect = 3e-62
 Identities = 188/768 (24%), Positives = 326/768 (42%), Gaps = 91/768 (11%)

Query: 25  EPEMLQRWYQEDLYGAIRQAKKGKKSFVLHDGPPYANGDIHIGHALNKILKDVIIKSKTL 84
           E ++ ++W  E  +  +   K+    +++   PPY  G +H+GH LN    D+I + K +
Sbjct: 12  EKKVQEKWEDEKTFKFLDDEKR--PPYIIDTPPPYPTGRMHLGHGLNWTYMDIIARFKRM 69

Query: 85  SGFDAPYIPGWDCHGLPIELMVEKKVGKPGQKVTAAEFREKCREYAAGQVEGQKESFKRL 144
           +G+D  +  GWDCHGLP E+ VE+        +   EFR  C E     VE  +   + L
Sbjct: 70  NGYDVLFPQGWDCHGLPTEVKVEELNNITKSDIDRHEFRRLCVELTDENVEKMRGQVRSL 129

Query: 145 GILGEWDKPYRTM--DFVTEANIIRALGKIADNGHLLKGFKPVHWCTDCGSALAEAEVEY 202
           GI  +WD+ Y TM  D+V ++    A  K+ + G + +G  PV+WC  C +A+A AE   
Sbjct: 130 GISIDWDREYITMNPDYVRKSQT--AFLKMYEKGLIYRGKHPVNWCPRCETAIAFAE--- 184

Query: 203 KNKVSPSIDVRFKAADEAAVLAKFGLAAGHEGKGDVSIVIWTTTPWTLPANRAVCLRADL 262
                    V ++         KF  A   E   +++         + P   A C+    
Sbjct: 185 ---------VEYQGRTSKLNYIKFPYAENSEKYLEIAT--------SRPELMAACVG--- 224

Query: 263 EYVLIQVEGEQPERIIVASELAKSVMDRAGIEHFHNLGFATGADLELVQFQHPFYSFTVP 322
             +++  E E+   ++      K+V                         + P ++  V 
Sbjct: 225 --IVVHPEDERYSDVV-----GKTV-------------------------KVPLFNQEVN 252

Query: 323 AILGDHVTTDSGTGVVHTAPGHGQEDFAVGQQYGLEVANPVGSNGVYLPDTELFAGQHVF 382
                 V  + GTGVV       + D     ++ LEV   +   G        +AG+   
Sbjct: 253 VYPDSDVEKEFGTGVVMVCTFGDKTDVTWVNRHKLEVKKAINEKGQLTEICGKYAGKKSD 312

Query: 383 KANDSVLEVLKEKGALLHHHAYEHSYPHCWRHKTPIIFRATPQWFVSMEQAGLREQALTA 442
            A   ++  L  +  ++     E +   CWR KTPI      QWFV++ +  L  +   A
Sbjct: 313 DARKEIISDLISENYMIKQEPLEQNVGSCWRCKTPIEIIVGDQWFVNVTK--LLNEVENA 370

Query: 443 IKGVHWMPDWGQSRIEGMVAGRP-EWCISRQRTWGVPIALFVHKETAELHPNSADLIEKV 501
              + W+P+  ++R+   +     +WCISRQR +  PI ++  K+  E+          +
Sbjct: 371 ANEISWVPEHMKARLMKWIEDMGWDWCISRQRLFATPIPVWYCKDCGEV----------I 420

Query: 502 AQLVEQKGIQAWWDLDTAELLGAEDAANYEKVLDTLDVWFDSGVTHSAVVD--ARQEFNG 559
               E   I    +       G  D      VLDT   W DS +T   +      +EF  
Sbjct: 421 VAKPEDLPIDPTKESPYTCKCGNSDLVAETDVLDT---WMDSSITPLVIAGWLEDEEFFK 477

Query: 560 AEADMYL--EGSDQHRGWFQSSLISSVAMKGKAPYKEVLTHGFVVDGQGRKMSKSIGNVV 617
               + L  +G D  R W   +++ S+A+ G+ P+ E++ +G V    G KMSKS GNVV
Sbjct: 478 KHYPVQLRPQGHDIIRTWAFYTMVRSLAITGEKPWDEIVINGMVFGEDGFKMSKSRGNVV 537

Query: 618 APQDVTNKLGADILRLWVASTDYTGEVAVSDEILKRSADAYRRIRNTARFFLANLNGFN- 676
            P ++T   GAD LRLW +++    +V  + + ++      R+I N  +F   N++    
Sbjct: 538 EPGEITKTYGADALRLWASNSTIGKDVPFAWKEVEYGGRFLRKIWNACKFAKMNISDETI 597

Query: 677 ---PTTDIIPVEDMVALDRWAVGRALAAQQEIIQAYQDYNLHAVVQRLMNFCSIEMGSFY 733
               +   I +E+ V  D W + +      ++ +   +Y ++ VV+ +  F   E    Y
Sbjct: 598 SKLKSLKSISIENPV--DLWILSKLNNLISKVSEDLGNYKINTVVE-IQKFLWHEFCDNY 654

Query: 734 LDVIKDRQYTAKRGGHAQRS---CQTALFFIVEALVRWMAPIMSFTAD 778
           ++++K R Y  +    AQ+     Q  L+ ++   V+ + P     A+
Sbjct: 655 IEMVKHRLYNKEESESAQQEKLMAQHTLYKVITESVKLLTPFTPHFAE 702