Pairwise Alignments
Query, 523 a.a., D-alanine--poly(phosphoribitol) ligase subunit from Phaeobacter inhibens DSM 17395
Subject, 5372 a.a., Amino acid adenylation from Pseudomonas syringae pv. syringae B728a
Score = 166 bits (420), Expect = 2e-44
Identities = 143/491 (29%), Positives = 219/491 (44%), Gaps = 37/491 (7%)
Query: 21 ALITSEEVISFGSLAGRVEGILRALDDM--PPGLSL-IVGHKEPDCIAAMIACAIAGRAF 77
A I +S+ L R + R L + PG S+ I+ + D +A+ +A +
Sbjct: 2692 AAIHDGVAVSYAELNNRANRLARHLLGLGVQPGDSVAILLERSHDLLASQLAVLKCAAVY 2751
Query: 78 AFADRSTPMDRLEQIASTAGASHILAFGQETLP--------GGLPVQTLKELPTRTPQLE 129
D + P++R + + A +L Q +L GL ++ LK P
Sbjct: 2752 VPLDVNAPVERQSFMIEDSRARVLLTHSQVSLTTGAQRVDLDGLTLERLKGTDLALPPQ- 2810
Query: 130 LKTALSEEGTFYVIFTSGSTGTPKGV--PISRGNYMALHRWYTPLLGAVRPEGAHVNHAS 187
S E Y+++TSGSTGTPKGV P + +A++ Y R A ++
Sbjct: 2811 -----SSESVAYIMYTSGSTGTPKGVLVPHRAISRLAINNGYADFNAQDRVAFA----SN 2861
Query: 188 MAFDMGMFDLWPTLSLGRPVHLLNHANNIMPRNNIRHLTRVDVASPASWASTPSLLQLMC 247
AFD D+W L G V ++ + + P N R L V+ W + Q
Sbjct: 2862 PAFDASTLDVWAPLLNGGCVVVIGQHDLLSPLNFQRLLLEQSVS--VLWMTAGLFHQYAS 2919
Query: 248 TDPQFTAENLPDLRFFVVGGEMLPGALVQELQTRFPQAQVLNGYGPSEATCATHLYPISV 307
E LR+ +VGG++L A++ + P +LNGYGP+EAT + Y I
Sbjct: 2920 G----LGEAFSRLRYLIVGGDVLDPAVIGRVLANNPPQHLLNGYGPTEATTFSATYEIV- 2974
Query: 308 ADAAADGPMKIGCAVGENRMQIIDADGEDVPAGEEGELVLSGPQVISHYLPQDHPANAAL 367
+ +G + IG VG +R+ ++D G+ P G GEL + G V YL +D
Sbjct: 2975 --SVGNGSIPIGKPVGNSRLYVLDNQGQPAPLGVGGELYIGGQGVARGYLHRDELTLEKF 3032
Query: 368 -----SQESGINTYRTGDLARLDVDGNLILLGRIDRQVKLLGNRIELNEIERIAEGYSAV 422
+ YRTGDL R DGNL LGR D QVK+ G R+EL EIE ++ V
Sbjct: 3033 VADPFDSDPQARLYRTGDLVRWRADGNLEYLGRNDEQVKIRGFRVELGEIEARLAEHAEV 3092
Query: 423 RKAVCLPQKDAAGRVTGLQLFVEPLAGATCERDGLFAHMGKHLSAQVLPRDLRIVSAFPV 482
R+AV L ++DA G + + + L +H+ L ++P + + P+
Sbjct: 3093 REAVVLCRQDAPGDKRLVAYVTAQQPETALDIEHLRSHLQGLLPDYMVPAAYVQLDSLPL 3152
Query: 483 TSNGKLDTRIL 493
T+NGKLD + L
Sbjct: 3153 TANGKLDRKAL 3163
Score = 164 bits (414), Expect = 1e-43
Identities = 153/518 (29%), Positives = 230/518 (44%), Gaps = 31/518 (5%)
Query: 18 DRPALITSEEVISFGSLAGRVEGI---LRALDDMPPGLSLIVGHKEPDCIAAMIACAIAG 74
D AL+ E+ +S+G L R + L AL P I + P I ++ +G
Sbjct: 503 DAAALVFEEQTLSYGELNARANQVAHRLLALGVRPDDRVAICVERGPAMIIGLLGILKSG 562
Query: 75 RAFAFADRSTPMDRLEQIASTAGASHILAF-GQETLPGGLPVQTLKELPTRTPQLELKTA 133
+ D + P++RL A T G S +A Q+++ LPV + + L+ + A
Sbjct: 563 AGYVPLDPAYPLERL---AYTLGDSAPVALLSQQSVQPALPVSDVPLIYLDDVDLQDERA 619
Query: 134 LSEEGTF------YVIFTSGSTGTPKGVPISRGNYMALHRWYTPLLGAVRPEGAHVNHAS 187
+ + + YVI+TSGSTG PKGV + N L G + + H S
Sbjct: 620 CNPQVSVRPSDLAYVIYTSGSTGLPKGVMVEHRNVARLFSATQDWFGFNEQDVWALFH-S 678
Query: 188 MAFDMGMFDLWPTLSLGRPVHLLNHANNIMPRNNIRHLTRVDVASPASWASTPSLLQLMC 247
AFD ++++W L G + ++ + P + L V TPS + +
Sbjct: 679 FAFDFSVWEIWGALLHGGRLLIVPQLVSRSPEDFYNLLCSAGVTV---LNQTPSAFRQLI 735
Query: 248 TDPQFTAENLPDLRFFVVGGEMLPGALVQELQTRFPQA--QVLNGYGPSEATCATHLYPI 305
Q E + LR + GGE L A+++ R A Q++N YG +E T YP+
Sbjct: 736 A-AQDDKEQVHSLRQVIFGGEALETAMLKPWYARKVNASTQLVNMYGITETTVHVTYYPL 794
Query: 306 SVADAAADGPMKIGCAVGENRMQIIDADGEDVPAGEEGELVLSGPQVISHYLPQDHPANA 365
DA G IG + + ++ ++DA G +P G GEL + G V YL +D
Sbjct: 795 QPEDARRVGASPIGKRIPDLQLYLLDAYGHPLPPGAVGELYVGGAGVARGYLNRDELNTT 854
Query: 366 ALSQES-----GINTYRTGDLARLDVDGNLILLGRIDRQVKLLGNRIELNEIERIAEGYS 420
+ G YR+GDL R DG+L LGR D QVK+ G RIEL EIE
Sbjct: 855 RFLDDPFASAPGARMYRSGDLGRWLPDGSLEYLGRNDEQVKIRGFRIELGEIEARLSACE 914
Query: 421 AVRKAVCLPQKDAAGRVTGLQLFVEPLAGATCERDGLFAHMGKHLSAQVLPRDLRIVSAF 480
VR AV + ++D G L +V AG + L + L+ +LP + AF
Sbjct: 915 GVRDAVVVVREDEPGD-QRLVAYVIGTAGQEPDATWLREQLRLSLAEHMLPSAFVSLEAF 973
Query: 481 PVTSNGKLDTRILLEGGSDLPTALPPSRDIVASDTGLM 518
P+T+NGKLD + L +PTA +R + GL+
Sbjct: 974 PLTANGKLDRKAL-----PVPTADAFARSQYEAPEGLV 1006
Score = 148 bits (374), Expect = 4e-39
Identities = 131/483 (27%), Positives = 209/483 (43%), Gaps = 36/483 (7%)
Query: 26 EEVISFGSLAGRVEGILRALDDMPPGLS---LIVGHKEPDCIAAMIACAIAGRAFAFADR 82
E + +G + R + L + G+ ++ + D + AM+A AG + D
Sbjct: 3746 EHCLHYGEMNHRANALAHHLISLGVGVDDRVAVMARRGLDTLVAMLAVLKAGAGYVPVDP 3805
Query: 83 STPMDRLEQIASTAGASHILAFGQETLPGGLPVQTLKELPTRTPQLELK------TALSE 136
S P +R+ + + + +L Q+ L +P + P+ + L+
Sbjct: 3806 SHPDERIAYLLADSAPKVVLT--QQALTSRVPETAAPVIAFDRPEWPQRLENPQVAGLNA 3863
Query: 137 EGTFYVIFTSGSTGTPKGVPISRGNYMALHRWYTPLLGAVRPEGAHV-NHASMAFDMGMF 195
YVI+TSGSTG PKGV + L W+ G G+H + A FD +
Sbjct: 3864 AHLAYVIYTSGSTGQPKGVMVEHRTVGNLIDWHCQAFGL--QAGSHTASVAGFGFDAMAW 3921
Query: 196 DLWPTLSLGRPVHLLNHANNIMPRNNIRHLTRVDVASPASWASTPSLLQLMCTDPQFTAE 255
++WP L G +HL + I L +A P A P+ + +
Sbjct: 3922 EVWPALCAGATLHL---PPADVGNEQIDALLDWWIAQPLQVAFLPTPVAEYAFSRNLSH- 3977
Query: 256 NLPDLRFFVVGGEMLPGALVQELQTRFPQAQVLNGYGPSEATCATHLYPISVADAAADGP 315
P LR ++GG+ L ++ Q R P V+N YGP+EAT + DG
Sbjct: 3978 --PTLRTLLIGGDRL-----RQFQ-RDPGFTVINNYGPTEATVVA-----TSGRLLPDGS 4024
Query: 316 MKIGCAVGENRMQIIDADGEDVPAGEEGELVLSGPQVISHYLPQDHPANAALSQE----- 370
+ IG + R+ ++D + VP G GEL ++G V YL + ++
Sbjct: 4025 LDIGKPITNTRVYLLDEHQQLVPLGVAGELYVAGEGVARGYLNRPEMTAERFLRDPFHCD 4084
Query: 371 SGINTYRTGDLARLDVDGNLILLGRIDRQVKLLGNRIELNEIERIAEGYSAVRKAVCLPQ 430
+ YRTGDLAR + DG L LGR D QVK+ G R+EL EIE + +A+ L +
Sbjct: 4085 ADARMYRTGDLARWNADGTLDYLGRNDDQVKIRGLRVELGEIEAQLSQLPGIEEALVLAR 4144
Query: 431 KDAAGRVTGLQLFVEPLAGATCERDGLFAHMGKHLSAQVLPRDLRIVSAFPVTSNGKLDT 490
+D G+ F+E + L A + L ++P ++A+P+T+NGK+D
Sbjct: 4145 EDEPGQPRLAAYFIERDGSLSMPVSELRAALLAVLPGYMVPSAFVRLAAWPLTANGKVDR 4204
Query: 491 RIL 493
R L
Sbjct: 4205 RAL 4207
Score = 134 bits (337), Expect = 9e-35
Identities = 125/457 (27%), Positives = 194/457 (42%), Gaps = 32/457 (7%)
Query: 62 DCIAAMIACAIAGRAFAFADRSTPMDRLEQIASTAGASHILA-------FGQETLPGGL- 113
+ IA ++ AG + D S P +RL + + +L F Q +P L
Sbjct: 1639 EMIAGLVGILKAGAGYVPLDPSYPEERLAYMLEDSAPKMLLTQRDLRERFAQAAMPVLLL 1698
Query: 114 -----PVQTLKELPTRTPQLELKTALSEEGTFYVIFTSGSTGTPKGVPISRGNYMALHRW 168
+ PT PQL L+ + Y+I+TSGSTG PKGV + + L W
Sbjct: 1699 EADARAENAIDSAPTTNPQL---AGLNAQHLAYLIYTSGSTGQPKGVAMPHAPLVNLMHW 1755
Query: 169 Y-TPLLGAVRPEGAHVNHASMAFDMGMFDLWPTLSLGRPVHLLNHANNIMPRNNIRHLTR 227
+ RP + A++ FD+ +++ TL G + L++ + R +H+
Sbjct: 1756 QIAQTVEHRRPRQRTLQFAALGFDVAFQEIFSTLCAGGELSLIHSDMRLDFRRLFQHICE 1815
Query: 228 VDVASP-ASWASTPSLLQLMCTDPQFTAENLPDLRFFVVGGEMLP-GALVQELQTRFPQA 285
+ + +L + + +P+ L D+ + GE L ++ R A
Sbjct: 1816 QRIERLYMPCIALQALAEAVVAEPEQPECLLQDV---ITAGEQLRITEPMRHFFARLNDA 1872
Query: 286 QVLNGYGPSEATCATHLYPISVADAAADGPMKIGCAVGENRMQIIDADGEDVPAGEEGEL 345
++ N YGP+E+ T + A P IG V R+ ++D VP G GEL
Sbjct: 1873 RLHNHYGPTESHVVTAMTLEGNPQAWPTLP-SIGQPVANTRIYLLDEHMRPVPVGVAGEL 1931
Query: 346 VLSGPQVISHYLPQDHP------ANAALSQESG---INTYRTGDLARLDVDGNLILLGRI 396
+ G V YL +D A+ ++E G Y+TGDL DGN+I +GR
Sbjct: 1932 YIGGVCVARGYLNRDDLTADRFIADPFATREDGNPCARLYKTGDLGCWQDDGNIIYMGRN 1991
Query: 397 DRQVKLLGNRIELNEIERIAEGYSAVRKAVCLPQKDAAGRVTGLQLFVEPLAGATCERDG 456
D Q+K+ G RIEL EIE Y +R A L ++D G + F A E D
Sbjct: 1992 DDQIKIRGFRIELGEIEARLGQYPGLRDAAVLAREDVPGEKRLVAYFSVQAGQALPEADH 2051
Query: 457 LFAHMGKHLSAQVLPRDLRIVSAFPVTSNGKLDTRIL 493
+ H+ L ++P + PV+ NGKLD R L
Sbjct: 2052 MRLHLQAVLPDYMIPAAYVHLEKLPVSPNGKLDRRAL 2088
Score = 127 bits (320), Expect = 8e-33
Identities = 128/490 (26%), Positives = 209/490 (42%), Gaps = 39/490 (7%)
Query: 21 ALITSEEVISFGSL---AGRVEGILRALDDMPPGLSLIVGHKEPDCIAAMIACAIAGRAF 77
A++ E+ +S+ L A R+ LR P I + P+ + ++A AG +
Sbjct: 4813 AVVHGEQRLSYRQLNERANRLAHALRKQGVQPDSRVGICVERGPEMVVGLLAILKAGGGY 4872
Query: 78 AFADRSTPMDRLEQIASTAGASHILA-FGQETLPGGLPVQTL-------KELPTRTPQLE 129
D + P+DR+ + + + +LA ++L + V + ++ + P++
Sbjct: 4873 VPLDPAYPVDRIAYMLQDSAPAAVLAQTATQSLLADVSVPVINLDQDSWQDESVQNPEVP 4932
Query: 130 LKTALSEEGTFYVIFTSGSTGTPKGVPISRGNYMALHRWYTPLLGAVRPEGAHVNHASMA 189
L+ Y+I+TSGSTG PKGV I N + W E + S+
Sbjct: 4933 ---GLTSVHLAYLIYTSGSTGLPKGVMIEHRNTVNFLTWAHSAFAGSALEKTLFS-TSLN 4988
Query: 190 FDMGMFDLWPTLSLGRPVHLLNHANNIMPRNNIRHLTRVDVASPASWASTPSLLQLMCTD 249
FD+ +++ + L+ G + ++ + + ++ L + PS L+ +
Sbjct: 4989 FDLAVYECFGPLTSGGSIKVVQNVLELQHGDHDIGLIN----------TVPSALKAL--- 5035
Query: 250 PQFTAENLPDLRFFV-VGGEMLPGALVQELQTRFPQAQVLNGYGPSEATCATHLYPISVA 308
+LP V V GE L +LV+ L + ++ N YGPSE T + +
Sbjct: 5036 --LEINSLPKSVHTVNVAGEALKRSLVENLFEKTGVQRLCNLYGPSETTTYSSWVAMDRE 5093
Query: 309 DAAADGPMKIGCAVGENRMQIIDADGEDVPAGEEGELVLSGPQVISHYLPQDHPANA--- 365
A IG VG + ++D + VP G GE+ + G V YL +D
Sbjct: 5094 QGFAP---HIGKPVGNTQFYLLDEQQQLVPLGVAGEIYIGGAGVARGYLNRDDLTAERFL 5150
Query: 366 --ALSQESGINTYRTGDLARLDVDGNLILLGRIDRQVKLLGNRIELNEIERIAEGYSAVR 423
S+ YRTGDL R DGN+ LGR D QVK+ G RIEL EI+ + AV
Sbjct: 5151 ADTFSRHPQARMYRTGDLGRYLPDGNIEYLGRNDDQVKIRGFRIELGEIDARLAKHPAVH 5210
Query: 424 KAVCLPQKDAAGRVTGLQLFVEPLAGATCERDGLFAHMGKHLSAQVLPRDLRIVSAFPVT 483
+AV ++D G + + A D L + L A ++P + A P+T
Sbjct: 5211 EAVVTAREDVPGDKRLVAYYTVLSAQTEPAIDSLRGWLLGQLPAYMVPVAYVRLDAMPLT 5270
Query: 484 SNGKLDTRIL 493
NGKLD + L
Sbjct: 5271 PNGKLDRKAL 5280