Pairwise Alignments
Query, 1624 a.a., Probable NAD-dependent glutamate dehydrogenase Gdh (NAD-Gdh) (NAD-dependent glutamic dehydrogenase) from Mycobacterium tuberculosis H37Rv
Subject, 1613 a.a., NAD-glutamate dehydrogenase from Vibrio cholerae E7946 ATCC 55056
Score = 1001 bits (2587), Expect = 0.0
Identities = 603/1549 (38%), Positives = 876/1549 (56%), Gaps = 74/1549 (4%)
Query: 96 LQVVAEHGGMLMDSVTVLLHRLGIAYAAILTPVFDVHRSPTGELLRIEPKAEGTSPHLGE 155
+++V L+DS+ + L RLG+A +L + R G + I + EG +
Sbjct: 95 VEIVLPDSPFLVDSIKMALSRLGLASHLMLNGPAHIARHDDGSIKSIN-QGEGQLTSM-- 151
Query: 156 AWMHVALSPAVDHKGLAEVERLLPKVLADVQRVATDATALIATLSELAGEVESNAGGRFS 215
H+ + + + E++ L +L D V D + L ++ ++E++
Sbjct: 152 --FHIEVDRLSSKEEMTELKNELLDILHDTALVVKDWKPMATKLEQVINQLEADKKQIPV 209
Query: 216 APDR-QDVGELLRWLGDGNFLLLGYQRCRVA----DGMVYGEGSSGMGVL----RGRTGS 266
+R Q+ + LRWLG+ NF +GY+ + D + +G+G+ R R+
Sbjct: 210 EAERLQETIQFLRWLGNHNFTFMGYKEFDLVEKNGDTELTPTKDTGLGLFSDNERVRSVK 269
Query: 267 RPRLTDDDKL-------LVLAQARVGSYLRYGAYPYAIAVREY-VDGSVV-EHRFVGLFS 317
+ D +L L+L + S + AY I ++++ G V+ EHRF GL++
Sbjct: 270 LSQFPDSARLEAKKPFLLILTKGNKQSRIHRPAYTDYIGIKKFDAKGKVIGEHRFTGLYT 329
Query: 318 VAAMNADVLEIPTISRRVREALAMAE-SDPSHPGQLLLDVIQTVPRPELFTLSAQRLLTM 376
A N V IP I +V LA + S+ + L ++++ PR EL + LL +
Sbjct: 330 SAVYNQSVEGIPLIREKVGRILAASGYRQGSYAYKALHNILENYPRDELLQAREEELLEV 389
Query: 377 ARAVVDLGSQRQALLFLRADRLQYFVSCLVYMPRDRYTTAVRMQFEDILVREFGGTR-LE 435
VV + + LF+R D F SC+VY+ ++RY T +R + + + + FG + +E
Sbjct: 390 GMGVVQMQDRDLLRLFVRKDPFGRFFSCMVYVTKERYNTELRRKTQQVFKQYFGCEQDVE 449
Query: 436 FTARVSESPWALMHFMVRLPEVGVAGEGAAAPPVDVSEANRIRIQGLLTEAARTWADRLI 495
FT SESP A H++VR+ + VDV +I+ L EA+ +W DRL
Sbjct: 450 FTTYFSESPLARTHYIVRVDNNNIN--------VDVK-----KIEQNLMEASTSWDDRLA 496
Query: 496 GAAAA--AGSVGQADAMHYAAAFSEAYKQAVTPADAIGDIAVITELTDDS------VKLV 547
A A S G + Y AF +YK+ V P A+ DI + L + + +L
Sbjct: 497 EAIVANFGESRGLPLSKEYQRAFPRSYKEDVMPGSALADIEHLEALDEHNKLGMLFYRLQ 556
Query: 548 FSERDEQGVAQLTWFLGGRTASLSQLLPMLQSMGVVVLEERPFSVTRPDGLPVWIYQFKI 607
+ +D + V +L + LS ++PML+++G+ V+ E P+ V + +G WI F +
Sbjct: 557 ETAKDSKAV-RLKLYHKDEPIHLSDVMPMLENLGLRVIGESPYEVVKANGQVYWILDFSM 615
Query: 608 SPHPTIPLAPTVAERAATAHRFAEAVTAIWHGRVEIDRFNELVMRAGLTWQQVVLLRAYA 667
+ + + RF +A AIW G +E D FN L++ A L+ ++V +LRAYA
Sbjct: 616 -----LHKSDKQVDLREARDRFQQAFAAIWAGELESDGFNRLILGASLSGREVSILRAYA 670
Query: 668 KYLRQAGFPYSQSYIESVLNEHPATVRSLVDLFEALFVPVPSGSASNRDAQAAAAAVAAD 727
+Y+RQ GFP+SQ YIE L+ HP + LVDLF F P G + ++
Sbjct: 671 RYMRQVGFPFSQHYIEDTLSHHPDLAQGLVDLFVRRFDPKYKGGEKGQ--AEIIKSLTEQ 728
Query: 728 IDALVSLDTDRILRAFASLVQATLRTNYFVTRQGSARCRDVLALKLNAQLIDELPLPRPR 787
+D + SLD DRI+R + ++ ATLRTNY+ + + L+LK+ I E+P P P
Sbjct: 729 LDQVQSLDDDRIIRRYMEMINATLRTNYYQLDEHKQN-KPWLSLKMKPSEIPEIPAPVPA 787
Query: 788 YEIFVYSPRVEGVHLRFGPVARGGLRWSDRRDDFRTEILGLVKAQAVKNAVIVPVGAKGG 847
+EIFVY+P +EGVHLR G VARGGLRWSDR++DFRTEILGLVKAQ VKN VIVPVGAKGG
Sbjct: 788 FEIFVYAPDIEGVHLRGGKVARGGLRWSDRQEDFRTEILGLVKAQQVKNTVIVPVGAKGG 847
Query: 848 FVVKRPPLPTGDPAADRDATRAEGVACYQLFISGLLDVTDNVDHATASVNPPPEVVRRDG 907
FV K+ L T RD AEG CY+ FI LLDVTDN+ V PP VVR D
Sbjct: 848 FVCKKQYLYT-----TRDEIFAEGQRCYKRFIRALLDVTDNI--IEGQVVPPKNVVRHDE 900
Query: 908 DDAYLVVAADKGTATFSDIANDVAKSYGFWLGDAFASGGSVGYDHKAMGITARGAWEAVK 967
DD YLVVAADKGTATFSD+AN V+ Y FWLGDAFASGGS GYDHKAMGITA+G WE+VK
Sbjct: 901 DDPYLVVAADKGTATFSDLANSVSAEYQFWLGDAFASGGSNGYDHKAMGITAKGGWESVK 960
Query: 968 RHFREIGIDTQTQDFTVVGIGDMSGDVFGNGMLLSKHIRLIAAFDHRHIFLDPNPDAAVS 1027
RHFRE+GID QT DFT +GIGDM+GDVFGNGMLLSKHIRL+AAF+H HIF+DP PD+A S
Sbjct: 961 RHFREMGIDCQTTDFTAIGIGDMAGDVFGNGMLLSKHIRLLAAFNHIHIFIDPTPDSASS 1020
Query: 1028 WAERRRMFELPRSSWSDYDRSLISEGGGVYSREQKAIPLSAQVRAVLGIDGSVDGGAAEM 1087
W ER R+F LPRSSW DY+ LIS+GGGV+SR+ KAI L+ +++ +L + +
Sbjct: 1021 WEERNRLFNLPRSSWEDYNPKLISKGGGVFSRKAKAITLTPEMQKMLNTKKTT------L 1074
Query: 1088 APPNLIRAILRAPVDLLFNGGIGTYIKAESESDADVGDRANDPVRVNANQVRAKVIGEGG 1147
AP LI+ IL+ VDLL+NGGIGTY+K+ E+ DVGDRAND +RV+ +V AK+IGEGG
Sbjct: 1075 APNELIKMILKMEVDLLWNGGIGTYVKSSIETHTDVGDRANDGLRVDGREVNAKIIGEGG 1134
Query: 1148 NLGVTALGRVEFDLSGGRINTDALDNSAGVDCSDHEVNIKILIDSLVSAGTVKADERTQL 1207
NLG+T GR+EF L GGR+NTD +DN GVDCSD+EVNIKI ++ LV+ G + +R Q+
Sbjct: 1135 NLGMTQRGRIEFALKGGRVNTDFVDNVGGVDCSDNEVNIKIFLNGLVANGDLTLKQRNQI 1194
Query: 1208 LESMTDEVAQLVLADNEDQNDLMGTSRANAASLLPVHAMQIKYLVAERGVNRELEALPSE 1267
LESM DEV +V+ D Q++ + + A SL+ I ++ ++R LE +P +
Sbjct: 1195 LESMKDEVGSIVIEDAYGQSESISVTEAQGVSLMKEQIRFIHHMEKAGYLDRALEHIPDD 1254
Query: 1268 KEIARRSEAGIGLTSPELATLMAHVKLGLKEEVLATELPDQDVFASRLPRYFPTALRERF 1327
+ + R G+GLT PEL+ LMA+ K+ LKEE+ + E+ + A +L YFPT LR +
Sbjct: 1255 ETLLERERQGMGLTRPELSVLMAYGKMALKEELASEEIAQDEFHAKQLVNYFPTELRGHY 1314
Query: 1328 TPEIRSHQLRREIVTTMLINDLVDTAGITYAFRIAEDVGVTPIDAVRTYVATDAIFGVGH 1387
++ +H LR EI+ T L N +V+ G + R+ E+ G + +D Y A I+G+G
Sbjct: 1315 AQQMVNHPLRVEIIATALANQMVNEMGCNFVTRLQEETGSSVVDIANAYAAAREIYGLGI 1374
Query: 1388 IWRRIRAANLPIALSDR---LTLDTRRLIDRAGRWLLNYRPQPLAVGAEINRFAAMVKAL 1444
+ ++R + IA S + RR + R RWLL R +V A + R+ VKA+
Sbjct: 1375 VLEKVRKLD-NIAQSSAQYDVMFLVRRTLRRLTRWLLRNRTGKPSVIAMVERYQEDVKAI 1433
Query: 1445 TPRMSEWLRGDDKAIVEKTAAEFASQGVPEDLAYRVSTGLYRYSLLDIIDIADIADIDAA 1504
T ++ + L ++ A + +G+ ++LA+ V+ YS+LDI +A I
Sbjct: 1434 TEQLDKVLVKEEIVEHNSMAENWIEKGIEKELAHYVARLSSLYSVLDISAVAKEKGIAVT 1493
Query: 1505 EVADTYFALMDRLGTDGLLTAVSQLPRHDRWHSLARLAIRDDIYGALRSLCFDVLA--VG 1562
+ A YF L DRL L ++ + W +LAR + R+D+ R L VL+ +
Sbjct: 1494 QTAKLYFHLGDRLSLHWFLKQINHQAVDNHWQALARASFREDLDWQQRQLTAQVLSSNLS 1553
Query: 1563 EPGESSEQKIAEWEHLSASRVARARRTLDDIRASGQKDLATLSVAARQI 1611
+ + E + +W + ++R L + + + A SVA R++
Sbjct: 1554 DAQQEIELALDKWLERNQVSISRWENILSEFKVGTVHEFAKFSVALREL 1602