Pairwise Alignments

Query, 1034 a.a., isoleucine--tRNA ligase from Methanococcus maripaludis S2

Subject, 940 a.a., isoleucyl-tRNA synthetase (NCBI ptt file) from Shewanella oneidensis MR-1

 Score =  392 bits (1007), Expect = e-113
 Identities = 254/849 (29%), Positives = 434/849 (51%), Gaps = 95/849 (11%)

Query: 8   NFRELDKKVKEYWKKENTYKKVKALNEHGPEYYFVDGPPYCSGAIHLGTAWNKIIKDTVL 67
           N    + ++ E W K+  Y++++        +   DGPPY +G+IH+G + NKI+KD ++
Sbjct: 20  NLANREPEMLERWTKDGLYQQIRDSRIGRTPFILHDGPPYANGSIHIGHSVNKILKDIIV 79

Query: 68  RFKRIQGYNVLDKAGWDMHGLPIEVKVENEFNIGSKKDIETKIGTQEFINKCKEFALNHL 127
           + K + G++     GWD HGLPIE+KVE +     +     KI   EF  +C+++A   +
Sbjct: 80  KSKTMSGFDAPYVPGWDCHGLPIELKVEQKVGKPGQ-----KISAAEFREECRKYAAEQV 134

Query: 128 GHMQGQFENLGVWLDFENAYMPIKRDYMEMGWWTLKKAHEKELLTKDLRSGYWCPRCETS 187
              +  F  LGV  D++N Y+ +          +L K  E   L K ++  +WC  C ++
Sbjct: 135 DGQREDFIRLGVLGDWQNPYLTMDFATEANIVRSLSKVIENGHLHKGVKPVHWCTDCGSA 194

Query: 188 LAEHEVRGEYKEVLDPSVYVKFKLEKS---------DEY-----ITIWTTTPWTLPSNML 233
           LAE EV  EY++   P++ V F    S          +Y     + IWTTTPWTLP+N  
Sbjct: 195 LAEAEV--EYEDKTSPAIDVAFTAVDSKAVAVKFGVSDYSHSVSMVIWTTTPWTLPANRA 252

Query: 234 VCVNPEFDYAYVNVEFENGTAETWIIAEKLVNDVMKK--AEKNNDISKFSISKVVKGDSL 291
           + ++PE DY+ V    ++G     I+A+ LV   M +  AE ++ ++K      +KG +L
Sbjct: 253 LSISPELDYSLVEF-VKDGVTHAVILADVLVEACMTRYGAESHSVLAK------IKGAAL 305

Query: 292 IGLKYIHPLLEENEKQQEFAKIENVHTIVPGDHVTLEGGTGLVHTAPGFGEDDFNIGKEH 351
             +++ HP L  +               + GDHVT + GTG+VHTAPG G+DDF +G+++
Sbjct: 306 ELVRFKHPFLAFDV------------PAILGDHVTTDAGTGVVHTAPGHGQDDFVVGQKY 353

Query: 352 NIPVYAPIDDNGKY--TDSIWKGTFVKDMDESVIETLISKNLLVNSGKVKHTYPHCWRCK 409
            + V  P+ DNG Y      + G  V   +++V+  L  K  L++    +H+YPHCWR K
Sbjct: 354 GLEVANPVGDNGVYKPDTEFFAGQHVFKANDNVVALLKEKGALLHHVAYRHSYPHCWRHK 413

Query: 410 TPLLFRATEQWFLSIS--KIKDSIIEQGKTVDWVPDWVKTRYVNGVSFVGDWNISRQRYW 467
           TP++FRAT QWF+S+    ++   + + + + W+PDW ++R    V    DW ISRQR W
Sbjct: 414 TPIIFRATPQWFISMDNHNLRKQALSEIEQIQWIPDWGQSRIEKMVENRPDWCISRQRTW 473

Query: 468 GIPLPIWICEECGNYEVIGSVDELKERANEKDVDLSDIHK-PAVDKITLTCSCGGKMKRT 526
           G+P+ +++  E        SV  +   AN   ++   I     +D   L      + ++ 
Sbjct: 474 GVPITLFVHRETEELHP-DSVSLMARVANR--IEQEGIQAWWDLDAAELLGEEAEQYRKV 530

Query: 527 PDVLDVWYDSGLAPYASIGSKKLK----KAQFITEGNDQVTKWFYSQHALSAVVFDDTSY 582
            D LDVWYDSG + +AS+ + + +          EG+DQ   WF S   +S  +     Y
Sbjct: 531 TDTLDVWYDSG-STFASVVAARPEFHGHGVDLYLEGSDQHRGWFMSSLMISTAMTGKAPY 589

Query: 583 EKCMMHGFTLDETGEKMSKSLGNIVSPDDVTEQYGADVLRFYLLSANKAWEDLRFSYSEM 642
           ++ + HGFT+D  G KMSKS+GN+++P  VT + GAD+LR ++ + + +         EM
Sbjct: 590 KQVLTHGFTVDGKGRKMSKSIGNVIAPQQVTNKLGADILRLWVAATDYS--------GEM 641

Query: 643 DETRSMLNTLWNSY---AFSANYMV--LDDFVPNNEYFKHVKDE---DAWILSRINTVAK 694
             +  +LN   ++Y     +A +++  L+ F P N+    V+D    D W + R   + +
Sbjct: 642 TVSDEILNRSADAYRRIRNTARFLLANLNGFDPKNDLVA-VEDMVALDRWAVRRAAALQQ 700

Query: 695 EAVEALEKPHLHVYTWALRDFILNDFSRWYIKLIRDRTWMEKNDVQ-KLSAYQTLYYVIM 753
           E +EA E+ + H+ T  L  F   +   +Y+ +I+DR +  K +   + S    L+++  
Sbjct: 701 EIIEAYEQYNFHIVTQKLMQFCSIELGSFYLDIIKDRQYTAKQEGHARRSCQSALFHIAE 760

Query: 754 KLISIMAPVTPHLSEEIYQNLKTE---------------------DMPESIFMNKLTIES 792
            ++  +APV    ++E++Q L  +                     D+ ++ + N LT+ +
Sbjct: 761 AMVRWIAPVLSFTADEVWQLLPGQRDAYVFTQEWYQGLQSITLDTDLSDAYWENLLTVRN 820

Query: 793 EFINETLEK 801
           E +N+ +E+
Sbjct: 821 E-VNKVIEQ 828