Pairwise Alignments
Query, 1060 a.a., bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase from Bifidobacterium breve UCC2003
Subject, 990 a.a., glutamate-ammonia-ligase adenylyltransferase (NCBI ptt file) from Shewanella oneidensis MR-1
Score = 199 bits (505), Expect = 1e-54
Identities = 224/922 (24%), Positives = 402/922 (43%), Gaps = 94/922 (10%)
Query: 86 KRLVTVLGVSDAMGKFMRFKPQLVEAAAVDNCNSHLFNHTQRRARLLKAVGADPDEPAMP 145
+ L TVLG+SD + + P+ + A D+ Q +L A +
Sbjct: 79 QELKTVLGLSDYIANQLTRSPEWINALFADDLQ-------QVERKLFDA------QLREQ 125
Query: 146 VASKDLAEAATALRSSYRN-QLAAIIAQDAVADDPTSIQPTISRELSDLADAALEGALAI 204
+AS + A L +RN Q+ +D + D S++ ++ +LS LA+A + GA
Sbjct: 126 LASATTEDMAKRLLRRFRNYQMVRFAWRDFL--DYASLEESLL-DLSALAEALVIGARDW 182
Query: 205 ARHET---------EGSEHVRFTIIGMGKLGAQELNYVSDVDLIYVVEPVDKDV------ 249
E + I+GMGKLG +ELN+ SD+DLI+ + V
Sbjct: 183 LYKEMCVQYGTPMDKAGNPQPLLILGMGKLGGRELNFSSDIDLIFTFPEHGETVGGRRSL 242
Query: 250 -DHQTLIRVGTKMGTMLQRVCQSAIMGVAEQPLWQIDGGLRPEGKDGALVRVLSSHKNYY 308
+ Q IR+G ++ +L ++ + +++D LRP G+ G LV S ++YY
Sbjct: 243 DNQQFFIRMGQRLVNLLDQITVDGFV-------FRVDMRLRPYGESGPLVVSFSGLEDYY 295
Query: 309 EQWAENWEFQALLKARPVAGDPDLGQAYMDMTRPFVWSASKRKNFVYDCQKMRKRVEDLI 368
++ +WE A++KAR + + RPFV+ + + +KM++ + +
Sbjct: 296 QEQGRDWERYAMVKARSLGPWNHFSDELHSLLRPFVYRRYIDFSAIESLRKMKQLIAQEV 355
Query: 369 PAPLKDREIKLGRGGLRDVEFTVQMLQLVHGRTDESLRTSNTLDSLQRLSEGGYVSRKQA 428
IKLG GG+R+VEF VQ QL+ G + SLR + ++ L G
Sbjct: 356 RRRQLTDNIKLGAGGIREVEFVVQSFQLIRGGREPSLRQQSLFGAMDTLYSLGQFEYLAV 415
Query: 429 VRMSQDYRFERVMEHRQQIWSLKRTHLFP----DLGRASVGGLEKKRDIDVDEFNQNQEL 484
+ Y R +E+ Q K+T P D R L+ +ID+ + +
Sbjct: 416 DELKHSYLLLRRVENLLQAIDDKQTQTLPNNALDWARLCY-VLDMTNEIDL-RTHIEAAM 473
Query: 485 RRLARAF--GLHPEELVDKYDDTRREVRHLHLDIYYRPMLPVNAQMENDQIVLSVEAAQE 542
++ R F + EE +K + ++ ++ D + +L Q+++D++ + +E
Sbjct: 474 AKIHRHFKATVGGEEGEEKAEHWTAQLWNVQQDDHAINLL-AEQQIDDDKLWPLLSRWRE 532
Query: 543 RFESIGFGDPDAAIRHVQALTAGVGRAAKINRIILPAVLQWLGEGQNPDMGLLNWRKLEE 602
G R + ++P +L L +P K+ E
Sbjct: 533 TVTKRSIGP----------------RGRETLDKLMPRLLDELLNQPSPSAAFEPVSKVLE 576
Query: 603 NFGTESGYLGFLRDSTSAAQRLCHILSNSRFLGDALNKSVESISWLGDDDNLQARTRGAL 662
T + YL L ++ A Q+L + S ++ L K + L D +L T +L
Sbjct: 577 QILTRTTYLELLCENPGARQQLVSLCCASPWIAVQLAKFPMLLDELIDPAHLYDTT--SL 634
Query: 663 DVQTGSALERFGSNI--NEFATSMRAMRRYEIE---RIGLSWMSGVISDSDSLKAMTDVY 717
D S L ++ + ++ M A+R++++ +I + ++GV+ +T +
Sbjct: 635 D-DYPSELRQYLLRVPEDDMEQQMEALRQFKLSQQLKIAAADVTGVLPVMQVSDHLTFLA 693
Query: 718 DAIIDASL--TW---AVRHQIAAFGVETAPAGITVIAMGRYGGREVNFSSDADAILIYRP 772
+AII+ + W AVRH + ++ E++ G VI G+ GG E+ + SD D + +Y
Sbjct: 694 EAIIEQVVMQAWQQVAVRHGVPSYLAESSDTGFAVIGYGKLGGIELGYGSDLDLVFLYEA 753
Query: 773 ADDADDGQANA--------FAKKVVEDLRNILQGPTTLEPKIELDLDLRPEGKNGPLVRS 824
++ + N F K+ + + ++ TT E+D+ LRP G +G +V
Sbjct: 754 PENMANSLTNGDRPIEVGHFYLKLAQRILHLFSTRTTSGELYEVDMRLRPSGASGLMVSE 813
Query: 825 YASCEEYYESWASTWERQALLRARYAAGDAELARDFLINIADPLRY--PTTELTEAELQN 882
A EY A TWE QAL+R+R+ GD LA F A L EL +A
Sbjct: 814 IARFGEYQAQEAWTWEHQALVRSRFVFGDNSLAVKFSQIRACVLEQSRDKDELKKA---- 869
Query: 883 IRKLKARMEAERLPRGVRRERHLKLGKGGLSDVEWTVQLMQLQHASDIKDLRV-NGTLEA 941
+R+++ +M + L + E LK GG++D+E+ Q + L +A + +L + + +
Sbjct: 870 VREMRQKMR-DHLLKVSEGEFDLKQSPGGITDIEFIAQYLVLANAHEYPELSIWSDNVRI 928
Query: 942 LDVLEAKKLISAIDAIQLRKAW 963
VL +L+ + A L +++
Sbjct: 929 FGVLAELELLPLMSAQHLTQSY 950
Score = 117 bits (293), Expect = 4e-30
Identities = 103/388 (26%), Positives = 177/388 (45%), Gaps = 27/388 (6%)
Query: 596 NWRKLEENFGTESGYLGFLRDSTSAAQRLCHILSNSRFLGDALNKSVESISWLGDDDNLQ 655
+W +L E + G + + Q L +L S ++ + L +S E I+ L DD LQ
Sbjct: 58 HWARLTEVWSE-----GLANLTAAQQQELKTVLGLSDYIANQLTRSPEWINALFADD-LQ 111
Query: 656 ARTRGALDVQTGSALERFGSNINEFATSM-RAMRRYEIERIGLSWMSGVISDSDSLKAMT 714
R D Q L + + A + R R Y++ R S +SL ++
Sbjct: 112 QVERKLFDAQLREQLA--SATTEDMAKRLLRRFRNYQMVRFAWRDFLDYASLEESLLDLS 169
Query: 715 DVYDAIIDASLTWAVRHQIAAFGVETAPAG----ITVIAMGRYGGREVNFSSDADAILIY 770
+ +A++ + W + +G AG + ++ MG+ GGRE+NFSSD D I +
Sbjct: 170 ALAEALVIGARDWLYKEMCVQYGTPMDKAGNPQPLLILGMGKLGGRELNFSSDIDLIFTF 229
Query: 771 RPADDADDGQANA----FAKKVVEDLRNILQGPTTLEPKIELDLDLRPEGKNGPLVRSYA 826
+ G+ + F ++ + L N+L T +D+ LRP G++GPLV S++
Sbjct: 230 PEHGETVGGRRSLDNQQFFIRMGQRLVNLLDQITVDGFVFRVDMRLRPYGESGPLVVSFS 289
Query: 827 SCEEYYESWASTWERQALLRARYAAGDAELARDFLINIADPLRYPTTELTEAELQNIRKL 886
E+YY+ WER A+++AR + G D L ++ P Y + + ++++RK+
Sbjct: 290 GLEDYYQEQGRDWERYAMVKAR-SLGPWNHFSDELHSLLRPFVY-RRYIDFSAIESLRKM 347
Query: 887 KARMEAERLPRGVRRER---HLKLGKGGLSDVEWTVQLMQLQHASDIKDLRVNGTLEALD 943
K + E VRR + ++KLG GG+ +VE+ VQ QL LR A+D
Sbjct: 348 KQLIAQE-----VRRRQLTDNIKLGAGGIREVEFVVQSFQLIRGGREPSLRQQSLFGAMD 402
Query: 944 VLEAKKLISAIDAIQLRKAWTLCTAARN 971
L + + +L+ ++ L N
Sbjct: 403 TLYSLGQFEYLAVDELKHSYLLLRRVEN 430
Score = 95.9 bits (237), Expect = 1e-23
Identities = 104/427 (24%), Positives = 177/427 (41%), Gaps = 35/427 (8%)
Query: 38 DERCAELLSVLAHACDPDVALSNFVDIVNAMQSSQRDLKHVIPDNNALKRLVTVLGVSD- 96
D+ LL L + P A ++ + + L+ + + A ++LV++ S
Sbjct: 548 DKLMPRLLDELLNQPSPSAAFEPVSKVLEQILTRTTYLELLCENPGARQQLVSLCCASPW 607
Query: 97 ---AMGKFMRFKPQLVEAAAVDNCNSHLFNHTQRRARLLKAVGADPDEPAMPVASKDLAE 153
+ KF +L++ A HL++ T L ++ + + V D+ +
Sbjct: 608 IAVQLAKFPMLLDELIDPA-------HLYDTTS-----LDDYPSELRQYLLRVPEDDMEQ 655
Query: 154 AATALRSSYRNQLAAIIAQDAVADDPTSIQPTISRELSDLADAALEGALAIA------RH 207
ALR +Q I A D P +S L+ LA+A +E + A RH
Sbjct: 656 QMEALRQFKLSQQLKIAAADVTGVLPVM---QVSDHLTFLAEAIIEQVVMQAWQQVAVRH 712
Query: 208 ETEG----SEHVRFTIIGMGKLGAQELNYVSDVDLIYVVEPVDKDVDHQTL----IRVGT 259
S F +IG GKLG EL Y SD+DL+++ E + + T I VG
Sbjct: 713 GVPSYLAESSDTGFAVIGYGKLGGIELGYGSDLDLVFLYEAPENMANSLTNGDRPIEVGH 772
Query: 260 KMGTMLQRVCQSAIMGVAEQPLWQIDGGLRPEGKDGALVRVLSSHKNYYEQWAENWEFQA 319
+ QR+ L+++D LRP G G +V ++ Y Q A WE QA
Sbjct: 773 FYLKLAQRILHLFSTRTTSGELYEVDMRLRPSGASGLMVSEIARFGEYQAQEAWTWEHQA 832
Query: 320 LLKARPVAGDPDLGQAYMDMTRPFVWSASKRKNFVYDCQKMRKRVED-LIPAPLKDREIK 378
L+++R V GD L + + + + + ++MR+++ D L+ + ++K
Sbjct: 833 LVRSRFVFGDNSLAVKFSQIRACVLEQSRDKDELKKAVREMRQKMRDHLLKVSEGEFDLK 892
Query: 379 LGRGGLRDVEFTVQMLQLVHGRTDESLRT-SNTLDSLQRLSEGGYVSRKQAVRMSQDYRF 437
GG+ D+EF Q L L + L S+ + L+E + A ++Q Y +
Sbjct: 893 QSPGGITDIEFIAQYLVLANAHEYPELSIWSDNVRIFGVLAELELLPLMSAQHLTQSYCW 952
Query: 438 ERVMEHR 444
R HR
Sbjct: 953 LRDENHR 959