MetaCyc Pathway: L-tryptophan degradation III (eukaryotic) in Cupriavidus basilensis FW507-4G11

MetaCyc Pathway: L-tryptophan degradation III (eukaryotic) in Cupriavidus basilensis FW507-4G11

Tryptophan 2,3-dioxygenase:
L-tryptophan + dioxygen→N-formyl-L-kynurenine (EC 1.13.11.11; 1.13.11.52)

Arylformamidase:
N-formyl-L-kynurenine + H₂O→L-kynurenine + formate + H⁺ (EC 3.5.1.9)

Kynurenine 3-monooxygenase:
L-kynurenine + NADPH + dioxygen + H⁺→3-hydroxy-L-kynurenine + NADP⁺ + H₂O (EC 1.14.13.9)

No genes

Kynureninase:
3-hydroxy-L-kynurenine + H₂O→3-hydroxyanthranilate + L-alanine + H⁺ (EC 3.7.1.3)

3-hydroxyanthranilate 3,4-dioxygenase:
3-hydroxyanthranilate + dioxygen→2-amino-3-carboxymuconate-6-semialdehyde (EC 1.13.11.6)

Aminocarboxymuconate-semialdehyde decarboxylase:
2-amino-3-carboxymuconate-6-semialdehyde + H⁺→(2Z,4E)-2-amino-6-oxohexa-2,4-dienoate + CO₂ (EC 4.1.1.45)

Aminomuconate-semialdehyde dehydrogenase:
(2Z,4E)-2-amino-6-oxohexa-2,4-dienoate + NAD⁺ + H₂O→(2Z,4E)-2-aminomuconate + NADH + 2 H⁺ (EC 1.2.1.32)

2-aminomuconate deaminase:
(2Z,4E)-2-aminomuconate + H₂O→(3E)-2-oxohex-3-enedioate + ammonium (EC 3.5.99.5)

(3E)-2-oxohex-3-enedioate + NADH + H⁺→2-oxoadipate + NAD⁺

No genes

2-oxoadipate + coenzyme A + NAD⁺→glutaryl-CoA + CO₂ + NADH (EC 1.2.1.105)

No genes

glutaryl-CoA + an oxidized electron-transfer flavoprotein + H⁺→(E)-glutaconyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.6)

No genes

(E)-glutaconyl-CoA + H⁺→crotonyl-CoA + CO₂ (EC 1.3.8.6)

No genes

crotonyl-CoA + H₂O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)

3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD⁺→acetoacetyl-CoA + NADH + H⁺ (EC 1.1.1.35)

Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information