MetaCyc Pathway: toluene degradation to 2-hydroxypentadienoate I (via o-cresol) in Cupriavidus basilensis FW507-4G11

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toluene + NADH + dioxygen + H+→2-methylphenol + NAD+ + H2O (EC 1.14.13.243)
RR42_RS32665
2-methylphenol + NADH + dioxygen + H+→3-methylcatechol + NAD+ + H2O (EC 1.14.13.243)
RR42_RS32665
Catechol 2,3-dioxygenase:
3-methylcatechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H+ (EC 1.13.11.2)
RR42_RS32655
RR42_RS34600
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + acetate + H+ (EC 3.7.1.25)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information