MetaCyc Pathway: cyclosporin A biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Alanine racemase:
L-alanine→D-alanine (EC 5.1.1.1; 5.1.1.10)
RR42_RS12035
Threonine ammonia-lyase:
L-threonine→2-oxobutanoate + ammonium (EC 4.3.1.19)
RR42_RS02315
RR42_RS05695
RR42_RS11110
RR42_RS33880
2-oxobutanoate + L-glutamate→(S)-2-aminobutanoate + 2-oxoglutarate
No genes
D-alanine + ATP + a [SimA non-ribosomal peptide synthase]→a [SimA NRPS]-intermediate 1 + AMP + diphosphate (EC 6.2.1.54)
No genes
a [SimA NRPS]-intermediate 1 + L-leucine + ATP + S-adenosyl-L-methionine→a [SimA NRPS]-intermediate 2 + S-adenosyl-L-homocysteine + AMP + diphosphate + 2 H+
No genes
L-leucine + a [SimA NRPS]-intermediate 2 + ATP + S-adenosyl-L-methionine→a [SimA NRPS]-intermediate 3 + S-adenosyl-L-homocysteine + AMP + diphosphate + 2 H+
No genes
a [SimA NRPS]-intermediate 3 + L-valine + ATP + S-adenosyl-L-methionine→a [SimA NRPS]-intermediate 4 + S-adenosyl-L-homocysteine + AMP + diphosphate + 2 H+
No genes
a [SimA NRPS]-intermediate 4 + (4R)-4-[(E)-2-butenyl]-4-methyl-L-threonine + ATP + S-adenosyl-L-methionine→a [SimA NRPS]-intermediate 5 + S-adenosyl-L-homocysteine + AMP + diphosphate + 2 H+
No genes
(S)-2-aminobutanoate + a [SimA NRPS]-intermediate 5 + ATP→a [SimA NRPS]-intermediate 6 + AMP + diphosphate + H+
No genes
a [SimA NRPS]-intermediate 6 + sarcosine + ATP→a [SimA NRPS]-intermediate 7 + AMP + diphosphate + H+
No genes
L-leucine + a [SimA NRPS]-intermediate 7 + ATP + S-adenosyl-L-methionine→a [SimA NRPS]-intermediate 8 + S-adenosyl-L-homocysteine + AMP + diphosphate + 2 H+
No genes
a [SimA NRPS]-intermediate 8 + L-valine + ATP→a [SimA NRPS]-intermediate 9 + AMP + diphosphate + H+
No genes
L-leucine + a [SimA NRPS]-intermediate 9 + ATP + S-adenosyl-L-methionine→a [SimA NRPS]-intermediate 10 + S-adenosyl-L-homocysteine + AMP + diphosphate + 2 H+
No genes
L-alanine + a [SimA NRPS]-intermediate 10 + ATP→a [SimA NRPS]-intermediate 11 + AMP + diphosphate + H+
No genes
a [SimA NRPS]-intermediate 11→cyclosporin A + H+ + a [SimA non-ribosomal peptide synthase]
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information