MetaCyc Pathway: clorobiocin biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


3-amino-4,7-dihydroxycoumarin + 4-hydroxy-3-prenylbenzoate + ATP→8-demethylnovobiocic acid + AMP + diphosphate + H+ (EC 6.3.1.15)
No genes
Proline--tRNA ligase:
L-proline + ATP + H+→(L-prolyl)adenylate + diphosphate (EC 6.1.1.15; 6.2.1.53)
RR42_RS17925
8-demethylnovobiocic acid + chloride + FADH2 + dioxygen→clorobiocic acid + FAD + 2 H2O
No genes
(L-prolyl)adenylate + a holo-[CloN5 prolyl-carrier protein]→L-prolyl-[CloN5 prolyl-carrier protein] + AMP + H+ (EC 6.2.1.53)
No genes
L-prolyl-[CloN5 prolyl-carrier protein] + 2 an oxidized electron-transfer flavoprotein + H+→1H-pyrrole-2-carbonyl-[CloN5 prolyl-carrier protein] + 2 a reduced electron-transfer flavoprotein (EC 1.3.8.14)
No genes
clorobiocic acid + dTDP-4-O-demethyl-β-L-noviose→4''-O-demethylclorobiocin + H+ + dTDP (EC 2.4.1.302)
No genes
1H-pyrrole-2-carbonyl-[CloN5 prolyl-carrier protein] + a holo-[CloN1 prolyl-carrier protein]→1H-pyrrole-2-carbonyl-[CloN1 prolyl-carrier protein] + a holo-[CloN5 prolyl-carrier protein]
No genes
4''-O-demethylclorobiocin + S-adenosyl-L-methionine→novclobiocin 104 + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.285)
No genes
novclobiocin 104 + 1H-pyrrole-2-carbonyl-[CloN1 prolyl-carrier protein]→5'''-demethylclorobiocin + a holo-[CloN1 prolyl-carrier protein]
No genes
5'''-demethylclorobiocin + methylcob(III)alamin + S-adenosyl-L-methionine→clorobiocin + 5'-deoxyadenosine + cob(III)alamin + L-methionine
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information