MetaCyc Pathway: pyrrolomycin biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Proline--tRNA ligase:
L-proline + ATP + H+→(L-prolyl)adenylate + diphosphate (EC 6.1.1.15; 6.2.1.53)
RR42_RS17925
(L-prolyl)adenylate + a holo-[L-prolyl-carrier protein]→an L-prolyl-[peptidyl-carrier protein] + AMP + H+ (EC 6.2.1.53)
No genes
an L-prolyl-[peptidyl-carrier protein] + 2 an oxidized electron-transfer flavoprotein + H+→a 1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] + 2 a reduced electron-transfer flavoprotein (EC 1.3.8.14)
No genes
a 1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] + 2 chloride + 2 FADH2 + 2 dioxygen→4,5-dichloro-1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] + 2 FAD + 4 H2O (EC 1.14.19.56)
No genes
4,5-dichloro-1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] + 3 malonyl-CoA + 2 NADPH + 5 H+→a 7-(4,5-dichloro-1H-pyrrol-2-yl)-3,5-dihydroxy-7-oxoheptanoyl-[PltL prolyl-carrier protein] + 3 CO2 + 3 coenzyme A + 2 NADP+
No genes
a 7-(4,5-dichloro-1H-pyrrol-2-yl)-3,5-dihydroxy-7-oxoheptanoyl-[PltL prolyl-carrier protein]→2-(4,5-dichloro-1H-pyrrole-2-carbonyl)phenol + 2 H2O + a holo-[L-prolyl-carrier protein]
No genes
2-(4,5-dichloro-1H-pyrrole-2-carbonyl)phenol + 2 chloride + 2 FADH2 + 2 dioxygen→pyrrolomycin C + 2 FAD + 4 H2O
No genes
pyrrolomycin C + nitrite + H+→pyrrolomycin G
No genes
pyrrolomycin C + chloride + FADH2 + dioxygen→pyrrolomycin D + FAD + 2 H2O
No genes
pyrrolomycin G + S-adenosyl-L-methionine→pyrrolomycin H + S-adenosyl-L-homocysteine + H+
No genes
pyrrolomycin D + S-adenosyl-L-methionine→pyrrolomycin J + S-adenosyl-L-homocysteine + H+
No genes
pyrrolomycin G→pyrrolomycin B
No genes
pyrrolomycin H + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→dioxapyrrolomycin + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
No genes
pyrrolomycin B→pyrrolomycin A + 4,6-dichloro-2-methylphenol
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information