MetaCyc Pathway: anteiso-branched-chain fatty acid biosynthesis in Cupriavidus basilensis FW507-4G11

Add experiment(s):


Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate
(EC 2.6.1.42)
RR42_RS25890
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH
(EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + a malonyl-[acp] + H+→a (4S)-4-methyl-3-oxohhexanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.300)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (4S)-4-methyl-3-oxohhexanoyl-[acp] + NADPH + H+→a (4S,3R)-3-hydroxy-4-methylhexanoyl-[acp] + NADP+
(EC 1.1.1.100)
RR42_RS04765
RR42_RS08710
RR42_RS09330
RR42_RS10255
RR42_RS10315
RR42_RS10320
RR42_RS11070
RR42_RS11550
RR42_RS13675
RR42_RS13690
RR42_RS14100
RR42_RS16365
RR42_RS16375
RR42_RS16800
RR42_RS17660
RR42_RS21765
RR42_RS21790
RR42_RS21865
RR42_RS22390
RR42_RS22710
RR42_RS22740
RR42_RS22810
RR42_RS23420
RR42_RS23690
RR42_RS24195
RR42_RS25125
RR42_RS25495
RR42_RS28045
RR42_RS28985
RR42_RS29305
RR42_RS31190
RR42_RS31670
RR42_RS31820
RR42_RS31835
RR42_RS31900
RR42_RS33070
RR42_RS33710
RR42_RS34680
RR42_RS35910
RR42_RS36415
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (4S,3R)-3-hydroxy-4-methylhexanoyl-[acp]→a (2E,4S)-4-methylhex-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
RR42_RS11820
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,4S)-4-methylhex-2-enoyl-[acp] + NADH + H+→a (4S)-4-methylhexanoyl-[acp] + NAD+
(EC 1.3.1.9)
RR42_RS03815
RR42_RS10070
RR42_RS10225
RR42_RS13390
RR42_RS25240
RR42_RS31520
RR42_RS31940
RR42_RS36330
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,4S)-4-methylhex-2-enoyl-[acp] + NADPH + H+→a (4S)-4-methylhexanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (4S)-4-methylhexanoyl-[acp] + a malonyl-[acp] + H+→a (6S)-6-methyl-3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
RR42_RS14090
RR42_RS31185
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (6S)-6-methyl-3-oxooctanoyl-[acp] + NADPH + H+→a (3R,6S)-3-hydroxy-6-methyloctanoyl-[acp] + NADP+
(EC 1.1.1.100)
RR42_RS04765
RR42_RS08710
RR42_RS09330
RR42_RS10255
RR42_RS10315
RR42_RS10320
RR42_RS11070
RR42_RS11550
RR42_RS13675
RR42_RS13690
RR42_RS14100
RR42_RS16365
RR42_RS16375
RR42_RS16800
RR42_RS17660
RR42_RS21765
RR42_RS21790
RR42_RS21865
RR42_RS22390
RR42_RS22710
RR42_RS22740
RR42_RS22810
RR42_RS23420
RR42_RS23690
RR42_RS24195
RR42_RS25125
RR42_RS25495
RR42_RS28045
RR42_RS28985
RR42_RS29305
RR42_RS31190
RR42_RS31670
RR42_RS31820
RR42_RS31835
RR42_RS31900
RR42_RS33070
RR42_RS33710
RR42_RS34680
RR42_RS35910
RR42_RS36415
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,6S)-3-hydroxy-6-methyloctanoyl-[acp]→a (2E,6S)-6-methyloct-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
RR42_RS11820
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,6S)-6-methyloct-2-enoyl-[acp] + NADH + H+→a (6S)-6-methyloctanoyl-[acp] + NAD+
(EC 1.3.1.9)
RR42_RS03815
RR42_RS10070
RR42_RS10225
RR42_RS13390
RR42_RS25240
RR42_RS31520
RR42_RS31940
RR42_RS36330
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,6S)-6-methyloct-2-enoyl-[acp] + NADPH + H+→a (6S)-6-methyloctanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (6S)-6-methyloctanoyl-[acp] + a malonyl-[acp] + H+→an (8S)-8-methyl-3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
RR42_RS14090
RR42_RS31185
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an (8S)-8-methyl-3-oxodecanoyl-[acp] + NADPH + H+→a (3R,8S)-3-hydroxy-8-methyldecanoyl-[acp] + NADP+
(EC 1.1.1.100)
RR42_RS04765
RR42_RS08710
RR42_RS09330
RR42_RS10255
RR42_RS10315
RR42_RS10320
RR42_RS11070
RR42_RS11550
RR42_RS13675
RR42_RS13690
RR42_RS14100
RR42_RS16365
RR42_RS16375
RR42_RS16800
RR42_RS17660
RR42_RS21765
RR42_RS21790
RR42_RS21865
RR42_RS22390
RR42_RS22710
RR42_RS22740
RR42_RS22810
RR42_RS23420
RR42_RS23690
RR42_RS24195
RR42_RS25125
RR42_RS25495
RR42_RS28045
RR42_RS28985
RR42_RS29305
RR42_RS31190
RR42_RS31670
RR42_RS31820
RR42_RS31835
RR42_RS31900
RR42_RS33070
RR42_RS33710
RR42_RS34680
RR42_RS35910
RR42_RS36415
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,8S)-3-hydroxy-8-methyldecanoyl-[acp]→a (2E,8S)-8-methyldec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
RR42_RS11820
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,8S)-8-methyldec-2-enoyl-[acp] + NADH + H+→an (8S)-8-methyldecanoyl-[acyl-carrier protein] + NAD+
(EC 1.3.1.9)
RR42_RS03815
RR42_RS10070
RR42_RS10225
RR42_RS13390
RR42_RS25240
RR42_RS31520
RR42_RS31940
RR42_RS36330
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,8S)-8-methyldec-2-enoyl-[acp] + NADPH + H+→an (8S)-8-methyldecanoyl-[acyl-carrier protein] + NADP+
(EC 1.3.1.10; 1.3.1.104)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
an (8S)-8-methyldecanoyl-[acyl-carrier protein] + a malonyl-[acp] + H+→a (10S)-10-methyl-3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
RR42_RS14090
RR42_RS31185
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (10S)-10-methyl-3-oxododecanoyl-[acp] + NADPH + H+→a (3R,10S)-3-hydroxy-10-methyldodecanoyl-[acp] + NADP+
(EC 1.1.1.100)
RR42_RS04765
RR42_RS08710
RR42_RS09330
RR42_RS10255
RR42_RS10315
RR42_RS10320
RR42_RS11070
RR42_RS11550
RR42_RS13675
RR42_RS13690
RR42_RS14100
RR42_RS16365
RR42_RS16375
RR42_RS16800
RR42_RS17660
RR42_RS21765
RR42_RS21790
RR42_RS21865
RR42_RS22390
RR42_RS22710
RR42_RS22740
RR42_RS22810
RR42_RS23420
RR42_RS23690
RR42_RS24195
RR42_RS25125
RR42_RS25495
RR42_RS28045
RR42_RS28985
RR42_RS29305
RR42_RS31190
RR42_RS31670
RR42_RS31820
RR42_RS31835
RR42_RS31900
RR42_RS33070
RR42_RS33710
RR42_RS34680
RR42_RS35910
RR42_RS36415
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,10S)-3-hydroxy-10-methyldodecanoyl-[acp]→a (2E,10S)-10-methyldodec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
RR42_RS11820
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,10S)-10-methyldodec-2-enoyl-[acp] + NADH + H+→a (10S)-10-methyldodecanoyl-[acp] + NAD+
(EC 1.3.1.9)
RR42_RS03815
RR42_RS10070
RR42_RS10225
RR42_RS13390
RR42_RS25240
RR42_RS31520
RR42_RS31940
RR42_RS36330
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,10S)-10-methyldodec-2-enoyl-[acp] + NADPH + H+→a (10S)-10-methyldodecanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (10S)-10-methyldodecanoyl-[acp] + a malonyl-[acp] + H+→a (12S)-12-methyl-3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
RR42_RS14090
RR42_RS31185
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (12S)-12-methyl-3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R,12S)-3-hydroxy-12-methyltetradecanoyl-[acp] + NADP+
(EC 1.1.1.100)
RR42_RS04765
RR42_RS08710
RR42_RS09330
RR42_RS10255
RR42_RS10315
RR42_RS10320
RR42_RS11070
RR42_RS11550
RR42_RS13675
RR42_RS13690
RR42_RS14100
RR42_RS16365
RR42_RS16375
RR42_RS16800
RR42_RS17660
RR42_RS21765
RR42_RS21790
RR42_RS21865
RR42_RS22390
RR42_RS22710
RR42_RS22740
RR42_RS22810
RR42_RS23420
RR42_RS23690
RR42_RS24195
RR42_RS25125
RR42_RS25495
RR42_RS28045
RR42_RS28985
RR42_RS29305
RR42_RS31190
RR42_RS31670
RR42_RS31820
RR42_RS31835
RR42_RS31900
RR42_RS33070
RR42_RS33710
RR42_RS34680
RR42_RS35910
RR42_RS36415
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,12S)-3-hydroxy-12-methyltetradecanoyl-[acp]→a (2E,12S)-12-methyltetradec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
RR42_RS11820
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,12S)-12-methyltetradec-2-enoyl-[acp] + NADH + H+→a (12S)-12-methyltetradecanoyl-[acp] + NAD+
(EC 1.3.1.9)
RR42_RS03815
RR42_RS10070
RR42_RS10225
RR42_RS13390
RR42_RS25240
RR42_RS31520
RR42_RS31940
RR42_RS36330
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,12S)-12-methyltetradec-2-enoyl-[acp] + NADPH + H+→a (12S)-12-methyltetradecanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (12S)-12-methyltetradecanoyl-[acp] + a malonyl-[acp] + H+→a (14S)-14-methyl-3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179)
RR42_RS14090
RR42_RS31185
Oleoyl-[acyl-carrier-protein] hydrolase:
a (12S)-12-methyltetradecanoyl-[acp] + H2O→(12S)-12-methyltetradecanoate + a soluble [acyl-carrier protein] + H+
(EC 3.1.2.14)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (14S)-14-methyl-3-oxohexadecanoyl-[acp] + NADPH + H+→a (3R,14S)-3-hydroxy-14-methylhexadecanoyl-[acp] + NADP+
(EC 1.1.1.100)
RR42_RS04765
RR42_RS08710
RR42_RS09330
RR42_RS10255
RR42_RS10315
RR42_RS10320
RR42_RS11070
RR42_RS11550
RR42_RS13675
RR42_RS13690
RR42_RS14100
RR42_RS16365
RR42_RS16375
RR42_RS16800
RR42_RS17660
RR42_RS21765
RR42_RS21790
RR42_RS21865
RR42_RS22390
RR42_RS22710
RR42_RS22740
RR42_RS22810
RR42_RS23420
RR42_RS23690
RR42_RS24195
RR42_RS25125
RR42_RS25495
RR42_RS28045
RR42_RS28985
RR42_RS29305
RR42_RS31190
RR42_RS31670
RR42_RS31820
RR42_RS31835
RR42_RS31900
RR42_RS33070
RR42_RS33710
RR42_RS34680
RR42_RS35910
RR42_RS36415
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,14S)-3-hydroxy-14-methylhexadecanoyl-[acp]→a (2E,14S)-14-methylhexadec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
RR42_RS11820
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,14S)-14-methylhexadec-2-enoyl-[acp] + NADH + H+→a (14S)-14-methylhexadecanoyl-[acp] + NAD+
(EC 1.3.1.9)
RR42_RS03815
RR42_RS10070
RR42_RS10225
RR42_RS13390
RR42_RS25240
RR42_RS31520
RR42_RS31940
RR42_RS36330
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,14S)-14-methylhexadec-2-enoyl-[acp] + NADPH + H+→a (14S)-14-methylhexadecanoyl-[acp] + NADP+
(EC 1.3.1.10; 1.3.1.104)
No genes
Oleoyl-[acyl-carrier-protein] hydrolase:
a (14S)-14-methylhexadecanoyl-[acp] + H2O→(14S)-14-methylhexadecanoate + a soluble [acyl-carrier protein] + H+
(EC 3.1.2.14)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information